硅钼蓝分光光度法测定进口玉米中的二氧化硅

介绍了用硅钼蓝分光光度法测定进口玉米中二氧化硅的含量,选择了最佳检测条件,进行检出限、精密度、加标回收率等方法的研究。本方法的检出限是0.033μg/mL,精密度0.70﹪,样品加标回收率93.61﹪。采用本方法测定粮食中的二氧化硅具有灵敏度高、准确、操作简便等优点。     

藏系绵羊钼诱导铜缺乏的研究

自2000年以来,青海省乌兰地区藏绵羊出现以异食、消瘦和贫血为特征的疾病。经分析该地区土壤、牧草和动物的矿物质元素含量,发现在患病区,土壤钼含量为（4.63±1.51）μg/g,牧草为（4.89±1.79）μg/g,极显著高于正常地区。患病动物血液平均铜含量为（0.23±0.07）μg/g,被毛铜平均含量为（3.27±0.71）μg/g,而正常区的动物为（0.97±0.23）μg/g和（6.78±1.57）μg/g,差异极显著;患病动物血液铜最高值为0.53μg/g,血液铜最低值为0.031μg/g。而正常地区藏绵羊血液铜含量最高值为1.33μg/g,血液铜最低值为0.83μg/g。同时患病动物血清铜蓝蛋白显著低于正常动物,并且有小红细胞贫血。结果表明,藏绵羊以异食、消瘦和贫血为特征的营养代谢疾病为钼诱导的次级铜缺乏。     

席夫碱合钼配合物作用大肠杆菌的微量热研究

用LKB-2277生物活性检测系统测定了两种席夫碱合钼(Ⅵ)配合物[MoO2(STZ)2]和[MoO2(VTZ)2]在37℃时对大肠杆菌作用的产热曲线;根据产热曲线求算了在席夫碱合钼(Ⅵ)配合物作用下,大肠杆菌生长代谢的速率常数k,抑制率I,传代时间G和半抑制浓度IC50等热力学参数.结果表明,席夫碱合钼(Ⅵ)配合物对大肠杆菌有抑制作用,[MoO2(STZ)2](IC50=72.22 mg·L-1)对大肠杆菌的抑制作用优于[MoO2(VTZ)2](IC50=143.57mg·L-1).     

小麦施钼效果与土壤氮、钼水平的关系研究

取湖北省几个主要麦产区土壤盆栽，研究小麦施钼效果与土壤Ｎ、Ｍｏ水平的关系。结果表明：小麦施钼效果与施Ｎ水平和土壤钼值密切相关，而与土壤有效钼含量相关不显著。在高Ｎ水平下施钼小麦增产显著；低Ｎ下施钼增产不稳定。用钼值指导小麦施钼明显优于传统的有效钼法。由此提出施Ｎ水平和土壤钼值是小麦施钼的两个重要有效条件。     

日粮中不同钼铜水平对肉牛血液生化指标的影响

本研究旨在探讨日粮中不同钼铜水平对肉牛血液生化指标的影响。选用性别一致、年龄、体重相近(340±10)kg的3头健康未去势公牛作为试验动物。试验采用3×3拉丁方设计,分2期进行,第一期日粮钼的水平为0.27 mg/kg DM,第二期钼的水平为5.27 mg/kg DM。每期日粮铜的添加量分别为:10 mg/kg(低铜组),25 mg/kg(中铜组),50 mg/kg(高铜组)3个水平。颈静脉采血测定血铜、血清铜蓝蛋白(ceruloplasmin,Cp)和血浆中过氧化物歧化酶(superoxide dismutase,SOD)的活性。试验结果表明:在同一钼水平下,随铜水平的增加,牛体内血铜含量、血浆中SOD活性和血清Cp的活性逐渐增加。其中钼水平为0.27 mg/kg时,这种变化不显著(P>0.05)。钼水平为5.27 mg/kg时,添加10 mg/kg铜,血铜含量和血浆中SOD活性显著小于其他2个铜水平(P<0.05),Cp活性小于其他2个铜水平,且差异极显著(P<0.01)。在同一铜水平下,低钼组2种酶的活性普遍高于高钼组。日粮钼水平为0.27 mg/kg时,添加10 mg/kg铜可满足机体需要;日粮钼水平为5.27 mg/kg时,添加25 mg/kg铜较适宜:牛在缺铜时,与血浆SOD的活性比较,血清Cp更宜作为评价铜营养缺乏的指标;铜满足机体需要后,血铜、血清Cp和血浆SOD均对铜和钼的变化不敏感。血铜和血清铜蓝蛋白与日粮铜水平呈强相关。     

Copper,Zinc and Molybdenum in Livers of Norwegian Cattle At Slaughter

The concentrations of copper, zinc and molybdenum were measured in samples of cattle liver from 10 slaughter-houses in Norway. A total of 335 samples were analysed. A clear accumulation of copper with age was found, the average copper level in the younger animals (≦ 3 years, n = 194) being 30 µg Cu/g liver wet weight, and in the older ones (> 3 years, n = 141) 59 µg Gu/g. The range in the copper values found was considerable, though significant differences between some of the districts were recorded. Copper concentrations were classified as low (≦10 µg Gu/g) in 9.6 % of the samples. Zinc showed no accumulation with age, nor were there any differences in zinc levels found in animals from different districts, the average level being 32 µg Zn/g liver wet weight. The picture was the same for molybdenum, no differences between age groups or districts being found. The average level was 1.0 µg Mo/g liver. There was no significant correlation between levels of copper, zinc or molybdenum.The supply of copper and zinc to cattle in Norway seems close to sufficient, but copper- and zinc-fortified mineral supplementation of cattle feed is still to be recommended. There seems to be no need for molybdenum supplementation in cattle.     

The Concentrations of Copper,Zinc and Molyrdenum in Swine Liver and the Relationship to the Distrirution of Solurle Copper- and Zinc-Rinding Proteins

The concentrations of copper, zinc and molybdenum were measured in liver samples from 21 normal slaughter pigs (average age about 6 months) and in 36 sows (average age about 2 years). The following mean values were found: Slaughter pigs: 15 ± 8 µg Cu/g, 45 ± 7 μg Zm/g and 1.0 ± 0.2 μg Mo/g wet weight; sows: 46 ± 70 μg Cu/g, 70 ± 26 μg Zn/g and 1.3 ± 0.3 μg Mo/g wet weight. The concentrations of all 3 elements were significantly higher in the sows than in the young pigs. There was no correlation between the concentrations of copper, zinc or molybdenum. The recorded copper levels in the slaughter pigs were in accordance with the levels of non-supplemented pigs given in the literature. The soluble hepatic copper- and zinc-binding proteins were separated into 3 different fractions by gel filtration. With increasing copper and zinc levels in the liver, a higher relative amount of these elements were found in the low molecular weight fraction.     

电感耦合等离子体光谱法测定豆科作物中钼含量

豆科作物样品经干灰化法处理,用ICP-AES法测定试液中痕量钼。试验中着重研究了共存元素对钼的干扰及消除。钼的样品检测限为0.82　μg.g-1,相对标准偏差RSD 2.1%(n=8),加标回收率为93.0%～98.6%。该方法已应用于百余个豆科作物的叶、茎、根、种子、植株样品的分析。     

钼硼对大豆氮磷钾吸收及产量的影响

采用田间小区试验,研究了施钼或硼对浙春３号大豆农艺性状,产量及氮磷钾吸收的影响,结果表明,施钼或硼明显促进了大豆干物质积累量的增长和对氮磷钾的吸收,促进了大豆株高和底荚高的增长,增加了大豆的百粒重,总荚数和单株籽粒重,提高了大豆产量。大豆植株Ｎ和Ｐ２Ｏ５含量粒期之前呈明显下降的趋势,鼓粒期之后有所回升;Ｋ２Ｏ５含量的波动幅度较大,大豆产量与底荚高和百粒重呈极显著正相关,与经济系数呈负相关,与株高、