覆膜滴灌条件下棉花根层土壤盐分时间稳定性研究

为揭示膜下滴灌棉田主根区土壤盐分的时间变异特性,基于2013—2014年田间实测数据,采用变异系数、平均相对偏差和标准差等方法研究了不同土层土壤盐分的时间稳定性,并进一步确定了可以反映各土层土壤平均含盐量的代表性测点。结果表明,研究区域0~40 cm土层土壤盐分随时间序列的变异性只有少数几个测点属于强变异,其余均属于中等变异;在棉花主根层(40 cm)内,土壤盐分的时间稳定性随土层深度增加呈现先增强后略微减弱趋势,在30 cm土层深度稳定性最强,平均相对偏差浮动范围最小、且其平均标准差最小;反映各土层平均含盐量的代表测点分布较为集中,可选取代表地块对区域土壤含盐量进行估算(决定系数R2为0.791 2~0.917 1)。棉田主根层土壤盐分时间稳定性研究有利于指导田间灌溉;选取少量且具有代表性的测点可为区域合理布设土壤盐分监测点提供理论依据。     

回龙电站尾水洞内衬钢板加固抗外压稳定分析

隧洞内衬钢板灌胶加固技术是在钢筋混凝土隧洞内壁通过植锚栓的方式安装薄钢板,而后在钢板与混凝土接触面之间灌注结构胶,使钢板与混凝土共同受力,满足钢筋混凝土隧洞结构加固的需要。在灌注结构胶的过程中,由于外部灌注压力的作用,薄钢板抗外压失稳问题将受到特别的关注。通过具体工程实例,详细介绍了薄钢板抗外压稳定计算的几种方法。     

具时滞能源价格模型的动力学性质

以滞量τ为分支参数,研究了具时滞的能源价格模型的动力学行为,这些行为包括：系统在平衡点附近的稳定性，局部Hopf分支的存在性，发生条件Hopf分支的方向，分支周期解的稳定性以及分支随参数变化其周期解的周期变化.最后通过数值模拟验证了理论分析结果，并用分支理论解释了能源价格模型产生且维持周期振荡的原因.     

Establishment and Analysis on Material Flow Model in Argo-animal Husbandry Ecosystem

This paper analyzed the material flow situation in argo-animal husbandry ecosystem by compartment model. This model was an important mean for investigating the whole structural characteristics in ecosystem. Based on this analysis, characteristics of material cycle and integrity in the system were mastered. As an example of natural conditions in Yonghe Village, Shuangcheng Township, Shuangcheng Municipal, Heilongjang Province, the system of linear differential equations in system was established by extracting each compartment and investigating material flow and stability of this model was proved by Lyapunov linear theory. The result showed that this system could not be interfered by initial value in the state of present, input and output.     

一类半线性变系数抛物型方程的二阶差分格式

本文对一类半线性变系数抛物型方程初边值问题建立了一个二阶差分格式,证明了差分格式解的存在唯一性、关于初值的无条件稳定性和在L∞范数下阶数为O(2τ+h2)的收敛性,最后给出的数值算例验证了理论结果。     

重组大肠杆菌不耐热肠毒素的表达及对小鼠胚胎稳定性的影响

为研究大肠杆菌不耐热肠毒素（LT）对小鼠胚胎稳定性的影响,运用大肠杆菌表达系统制备了具有生物活性的重组大肠杆菌LT,用重组LT通过腹腔注射处理妊娠小鼠,分析了LT对小鼠胚胎稳定性的影响。首先采用PCR方法从产毒大肠杆菌菌株44815基因组中扩增LT的A、B亚基基因,将其插入到pET-20b（＋）原核表达载体pelB信号肽的下游,分别构建成LTA和LTB分泌型表达载体;将载体分别转化大肠杆菌BL21（DE3）pLysS,在IPTG的诱导下进行表达,利用Ni-NTA琼脂糖凝胶从细菌周质释放液中提取和纯化重组LTA和LTB蛋白;利用细胞毒性试验检测重组蛋白的生物活性。然后用制备的重组LT注射妊娠6d的小鼠,连续注射3d后,统计小鼠的胚胎存活率。同时用ELISA方法检测小鼠血清中与胚胎稳定性密切相关的Th1型细胞因子（IFN-γ、IL-2）和Th2型细胞因子（IL-4、IL-10）及IL-β的表达水平。结果表明,采用分泌性表达策略实现了重组LT在大肠杆菌中的高效分泌表达,LTA和LTB的表达量分别达68、62mg/L。表达的重组LT具有明显的细胞毒性作用;用LT处理妊娠小鼠,胚胎的存活率为32%,明显低于对照组;小鼠血清中Th1型细胞因子IFN-γ和IL-2的含量明显高于对照组（P〈0.01）,分别为对照组的2、3倍。同时细胞因子IL-1β为对照组的2倍（P〈0.01）,而稳定胚胎发育的Th2型细胞因子IL-4、IL-10没有明显变化（P〉0.05）。据此推测,LT可能与大肠杆菌性肠炎引起妊娠家畜流产有关,LT对胚胎稳定性的影响除与LT的直接毒性有关外,可能还与LT介导的免疫调节异常有关。     

Lethal Effects of Elevated pH and Ammonia on Early Life Stages of Hybrid Striped Bass

Hybrid striped bass larvae (striped bass, Morone saxatilis, ♀ x white bass, M. chrysops, ♂) are often stocked into fertile culture ponds. High rates of photosynthesis may result in elevated pH which can be lethal to fry and can affect the toxicity of ammonia. Six-hour static toxicity tests were performed on hybrid larvae of various ages to determine the toxicity of elevated pH and the effects of elevated pH on ammonia toxicity. Six-hour mortality threshold pH's with no measurable ammonia were estimated for various age larvae and found to be between pH's 9.8 and 10.2 for D2 (Dl is day of hatch), between 9.0 and 9.4 for D4, between 8.8 and 9.2 for D13, and between 9.2 and 9.4 for D20 fish. The addition of 0.7% NaCl had no effect on the toxicity of elevated pH to D2 or D4 fish but caused a significant reduction in the mortality of Dl3 and D20 fish exposed to high pH. The tolerance to elevated pH decreased as ammonia concentration increased. Sub-lethal un-ionized ammonia concentrations increased the toxicity of elevated pH, suggesting an interaction of pH and un-ionized ammonia toxicity.     

不同采伐强度对闽西常绿阔叶林林分结构稳定性的影响

应用多样性分析方法和改进后的 M.Godron 稳定性测定方法研究常绿阔叶林5种不同强度采伐(弱度9.8%、中度24.5%,强度46.8%和极强度72.3%和皆伐)作业10 a后林分结构稳定性.结果表明,择伐林地林分处于稳定状态,随着择伐强度的增强,林分的稳定性开始逐渐降低.皆伐林地林分处于不稳定状态.对于常绿阔叶林的科学经营,从稳定性出发,应尽量采取择伐方式,尤其是中低强度的择伐方式.     

Study on the Stability of Blue-tongue Virus after Long Time Storage in Cold Room

In order to understand the stability of blue-tongue virus (BTV)storage in cold room for long time, 18 tissue samples which were kept in 4℃ for 10 to 15 years were test by Real-time PCR, and 10 blood samples which were kept in 4℃ for 15 years were subjected to virus isolation process, then was proved by monoclonal antibody staining,research results showed all old samples were positive in Real-time PCR, further more, 5 live virus were isolated from 10 old blood samples. The results suggested the stability of BTV was higher than we thought before.     

一个捕食者和食饵均具有阶段结构的捕食模型的稳定性

讨论一个捕食者和食饵均具有阶段结构的捕食模型的稳定性．通过分析特征方程，运用Hurwitz判定定理，讨论了该模型非负平衡点的局部稳定性；通过构造适当的Lyapunov泛函，运用LaSall不变集原理，讨论了该模型的非负平衡点的全局稳定性，从而得到了该生态系统永久持续生存与灭绝的充分条件