‘小偃60’在不同水氮条件下的用水特性

以过筛土壤为培养基础,以圆形桶为容器,设置了3个水分梯度(W0、W1、W2,分别为田间持水量的50%、65%、80%)、4个氮素梯度(N0、N1、N2、N3,施氮量分别为每桶0 g·kg?1、0.10 g·kg?1、0.20 g·kg?1、0.29 g·kg?1),探究冬小麦新品系‘小偃60’干物质积累与分配、水分利用效率、光合参数的变化规律,为其推广及高产高效栽培提供依据。结果表明,水、氮均对冬小麦生物量及产量、叶片叶绿素含量、叶水势、叶片净光合速率等有显著影响,且水对籽粒产量的主效应大于氮肥。水分和氮肥适宜处理(W2N2)比水分和氮肥轻度亏缺处理(W1N1)增产66.03%,W1N1比水分和氮肥严重亏缺(W0N0)增产153.30%。在相同水分处理下,冬小麦叶绿素SPAD值随着施氮量的升高而升高,而在相同施氮量条件下,水分严重亏缺处理(W0)和水分适宜处理(W2)的冬小麦叶绿素SPAD值均低于水分轻度亏缺处理(W1)。水分对叶片水势为正效应,表现为随着土壤含水量的增加显著升高到不明显升高趋势。光合速率(Pn)、蒸腾速率(Tr)、瞬时水分利用效率(WUEi)均随着施氮量的增加而升高,Pn、Tr随土壤水分提高而上升,WUEi却随土壤水分提高而下降。在低氮(N1)或者不施氮肥(N0)条件下,产量水分利用效率(WUEy)随水分的增加表现先升高而后变化不明显的变化趋势;在适宜氮肥(N2)和高氮(N3)条件下,WUEy随着水分的增加而升高。通过对试验结果的分析得出如下结论:1合理的水氮管理使‘小偃60’的籽粒产量及水分利用效率(WUE)维持在较高水平,过多水肥均引起产量及WUE下降;2不同水氮下籽粒产量、水分利用效率均与叶片净光合速率显著正相关;3‘小偃60’在河北环渤海地区更适合在低水中肥条件下种植。     

盐胁迫对刺槐混栽苗光合特性的影响

以刺槐、榆树、臭椿、绒毛白蜡、杨树为试材,利用不同浓度盐溶液模拟盐胁迫条件,研究了盐胁迫对刺槐混栽苗光合特性的影响。结果表明：低盐胁迫下刺槐混栽苗的Pn、Ci、Cond同步下降,且相关性较高;而随着胁迫的增强,三者又有所增高,在高盐胁迫下（200mmol/L）又开始下降,但盐胁迫对不同混栽苗光合作用的伤害作用不同。     

Light-stimulated 14CO2 uptake and acetylene reduction by bacteriochlorophyll containing stem nodule isolate BTAi 1

Summary Bradyrhizobial strain BTAi 1 nodulates both stems and roots of Aeschynomene spp. Previous work has shown that it contains bacteriochlorophyll a and forms photosynthetic reaction centers, and has provided indirect evidence of photosynthesis by bacteroids within stem nodules. Here we report physiological and biochemical characteristics of BTAi 1 ex planta, which also suggest the presence of photosynthetic activity. Light-stimulated uptake of ¹⁴CO₂ by BTAi 1 was detected at all stages of growth. Inhibitors of photosynthesis, 1,10-orthophenanthroline and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and the uncoupler NH₄Cl, immediately suppressed light-driven ¹⁴CO₂ uptake and increased O₂ uptake. BTAi 1 is strictly aerobic and was unable to grow without organic C even in the light; also, it was unable to grow chemoautotrophically in an atmosphere enriched with H₂ and CO₂. In micro-aerobic conditions, strain BTAi 1 expressed acetylene reducing activity ex planta in an N-free medium. The highest rates of light-stimulated ¹⁴CO₂ uptake and acetylene-reducing activity occurred during the exponential and early stationary phases of growth. Acetylene-reducing rates at a low glucose concentration were increased following a light-dark cycle in comparison with continuous dark conditions.     

UV-B辐射胁迫对水稻叶绿素荧光动力学的影响

研究结果表明，增强的紫外线－B（UV－B，280－320nm）辐射会导致水稻植株矮化4.71%-16.59%、叶面积变小、干物质生产量减少11.79%-60.57%。究其原因主要是叶片光合作用色素叶绿素a、叶绿素b和类胡萝卜素含量的降低和叶绿素a荧光动力学参数的改变，光合系统Ⅱ受到破坏，光合作用能力下降、生长发育受阻。     

Effects of substrate sterilization,fungicide treatment,and mycorrhization helper bacteria on ectomycorrhizal formation of pedunculate oak (Quercus robur) inoculated with Laccaria laccata in two peat bare-root nurseries

Summary Pedunculate oak seedlings (Quercus robur) inoculated with the ectomycorrhizal fungus Laccaria lacata were grown for 1 year on fertilized sphagnum peat in two nurseries. Three factors affecting microbial populations in the substrate were studied, fungicide treatment of the seeds, peat disinfection before sowing (methyl bromide or steam pasteurization), and inoculation with mycorrhization helper bacteria. Treatment of acorns with Iprodione had no depressive effect on mycorrhiza formation. Both disinfection techniques were equivalent, stimulating or depressing mycorrhiza formation depending on the initial microflora in the peat. The introduction of two previously selected mycorrhization helper bacteria (one Pseudomonas fluorescens and one unidentified fluorescent pseudomonad), isolated from L. laccata sporocarps associated with Douglas fir—L. laccata ectomycorrhizas in other nurseries, significantly increased the mycorrhizal rate from 30 to 53% of the short roots. The implications of these results for the controlled mycorrhization of planting stocks and the specificity of mycorrhization helper bacteria are discussed.     

Spatial changes of soil fungal and bacterial biomass from a sub-alpine coniferous forest to grassland in a humid, sub-tropical region

 Fungal and bacterial biomass were determined across a gradient from a forest to grassland in a sub-alpine region in central Taiwan. The respiration-inhibition and ergosterol methods for the evaluation of the microbial biomass were compared. Soil fungal and bacterial biomass both significantly decreased (P<0.05) with the shift of vegetation from forest to grassland. Fungal and bacterial respiration rates (evolved CO₂) were, respectively, 89.1 μl CO₂ g^–1 soil h^–1 and 55.1 μl CO₂ g^–1 soil h^–1 in the forest and 36.7 μl CO₂ g^–1 soil h^–1 and 35.7 μl CO₂ g^–1 soil h^–1 in the grassland surface soils (0–10 cm). The fungal ergosterol content in the surface soil decreased from the forest zone (108 μg g^–1) to the grassland zone (15.9 μg g^–1). A good correlation (R ²=0.90) was exhibited between the soil fungal ergosterol content and soil fungal CO₂ production (respiration) for all sampling sites. For the forest and grassland soil profiles, microbial biomass (respiration and ergosterol) declined dramatically with depth, ten- to 100-fold from the surface organic horizon to the deepest mineral horizon. With respect to fungal to bacterial ratios for the surface soil (0–10 cm), the forest zone had a significantly (P<0.05) higher ratio (1.65) than the grassland zone (1.05). However, there was no fungal to bacterial ratio trend from the surface horizon to the deeper mineral horizons of the soil profiles. Received: 30 March 2000     

氮肥运筹和栽培方式对杂交籼稻II优498结实期群体光合特性的影响

以杂交籼稻II优498为材料, 在温江和汉源两种生态条件下, 研究了不同氮肥运筹方式对宽窄行、三角形、扩行减株稀植和抛秧4种栽培方式水稻结实期群体光合生产的影响。结果表明, 随穗肥施用比例的增加, 结实期冠层透光特性得到改善, 抽穗期茎鞘干重、高效叶面积率和有效叶面积率等群体质量指标得到提高, 抽穗后叶面积指数(LAI)和呼吸占群体光合的相对比例(CR/TCAP)有所降低, 而群体光合速率(CAP)在两地的变化趋势并不一致, 温光条件较差的温江点表现为先升后降, 而温光条件较好的汉源点则呈下降趋势。当氮肥运筹为6∶3∶1时, 宽窄行和三角形栽培有利于改善冠层透光特性和群体质量, CAP升高的同时结实中后期CR/TCAP增幅减小, 群体光合生产能力得到提高;当氮肥运筹比例为5∶2∶3时, 扩行减株稀植栽培透光性能和群体质量的增幅最大, CAP升高的同时CR/TCAP并未增加甚至有所减少, 群体光合生产能力高于其他栽培方式;当氮肥运筹比例为4∶1∶5时, 抛秧栽培在温江点有利于改善群体质量和增加光能截获, 结实中后期CAP显著提高的同时CR/TCAP降低, 群体光合生产优势明显, 但温光条件改善后此优势并无体现。因此, 提高水稻群体光合生产能力需结合当地温光条件并针对栽培方式采用适宜的氮肥管理措施。     

玉米和高粱用于碳同化和光呼吸的电子效率估算

为了探讨C₄植物碳同化和光呼吸的电子效率,运用Li-6400光合仪同时测定玉米和高粱在30℃和380 μmol CO₂ mol^-1下叶片的气体交换和叶绿素荧光,结果表明,直角双曲线修正模型可较好地拟合所测的光响应曲线和快速光曲线,其拟合值与实测值较为一致。在此基础上算得玉米和高粱在光呼吸条件下参与碳同化的电子流分别为198.60 μmol m^-2 s^-1和178.00 μmol m^-2 s^-1,所占比率分别为75.34%和74.81%;参与光呼吸的电子流分别为7.04 μmol m^-2 s^-1和7.84 μmol m^-2 s^-1,所占比率分别为2.67%和3.29%。而根据Valentini和Epron的方法算得玉米和高粱碳同化的电子流分别为210.45 μmol m^-2 s^-1和188.54 μmol m^-2 s^-1,所占比例分别为82.68%和79.24%;参与光呼吸的电子流则分别为45.67 μmol m^-2 s^-1和49.40 μmol m^-2 s^-1,所占比率分别为17.32%和20.76%。以前法研究表明,玉米和高粱在光呼吸条件下,来自PSII的电子除流向光呼吸和碳还原外,还存在其他消耗电子的途径,证明其他消耗电子的途径并不能被忽略或其他途径所消耗电子的量并不是常数。后法过高地估算了玉米和高粱叶片中来自PSII的电子用于光呼吸的消耗量。两法的结果相差6倍左右。这对重新评估光呼吸在植物的光保护中所起的作用提供了理论依据。     

田间条件下转玉米C4型PEPC基因小麦的光合生理特性

为了检验转PEPC基因小麦是否具有C₄光合生理特性,以转PEPC基因小麦和对照周麦19为试验材料,分别于抽穗期、开花期、花后第7天和花后第15天测定其单株旗叶的气体交换参数日变化,对净光合速率日变化进行单位日光合总量分析, 并且对净光合速率日变化与单株气孔导度、胞间CO₂浓度、蒸腾速率日变化的相关性进行了分析;在花后第15天测定其单株叶绿素荧光参数,并在成熟期调查了单株产量性状。与对照相比,转基因株系在4个测定时期的旗叶净光合速率明显提高,尤其在花后第15天,单位日光合总量较对照提高29.1%和23.3%,气孔导度和蒸腾速率均明显增加,胞间CO₂浓度降低;花后第15天12:00与8:00相比,转PEPC基因小麦的F_v/F_m、q_p、NPQ、Φ_PSII的变幅均小于对照;单茎重、千粒重、单穗重和收获指数较对照显著增加。以上结果表明,转PEPC基因小麦材料在田间条件下光合特性明显优于对照,且具有提高小麦产量水平的潜力。