猪IL-12p40竞争定量RT-PCR检测方法的建立

根据GenBank中猪IL-12p40的序列设计引物,采用RT-PCR技术扩增377 bp的部分基因片段,并克隆到pGEM-T Easy载体中,构建成含IL-12p40部分基因片段的重组质粒. 利用反向PCR技术构建与扩增377 bp片段共用同一对引物但扩增255 bp片段的竞争重组子. 通过重组质粒与竞争重组子竞争定量PCR方法(qc-PCR),建立了猪IL-12p40的标准竞争曲线和直线回归方程.     

制造企业ERP软件产品选型技术

提出了一种制造企业ERP软件产品选型技术,建立了ERP软件选型的评价过程模型,依据平衡计分卡原理构建了ERP软件选型评价体系,运用标杆原理确定选型的参考数列,根据层次分析法给出了相对权重,在此基础上运用灰色系统原理实现了软件的优先级排序,最后给出了相关实例分析.应用表明,该方法具有全面性、实用性及可操作性的特点.     

电针“委中”对多裂肌损伤模型大鼠钙调蛋白信号通路的影响

目的 探讨多裂肌损伤后,电针"委中"干预对多裂肌损伤过程中钙调蛋白信号通路的影响。方法 SD大鼠随机分为空白组、模型1 d组、模型3 d组、电针1 d组、电针3 d组,每组8只。电针组双侧"委中"电针,于治疗的第1、3天各组同步取材,采用酶联免疫吸附法检测多裂肌、脊髓、海马组织中CaM、CaMKⅡ、iNOS的含量。结果 模型1 d和3 d组,多裂肌、脊髓、海马CaM、CaMKⅡ含量显著高于空白组（P<0.01）;多裂肌、脊髓、海马iNOS含量高于空白组（P<0.05或P<0.01）。电针1 d后,多裂肌、脊髓、海马CaM、CaMKⅡ、iNOS含量显著低于模型1 d组（P<0.05或P<0.01）。电针3 d后,多裂肌、脊髓、海马CaM、CaMKⅡ、iNOS含量低于模型3 d组（P<0.05或P<0.01）。电针3 d后,脊髓、海马CaM、CaMKⅡ及多裂肌CaMKⅡ含量显著低于电针1 d组（P<0.01）,多裂肌CaM、iNOS和脊髓、海马iNOS含量与电针1 d组比较差异无统计学意义（P>0.05）。结论 多裂肌损伤早期,电针"委中"干预可通过抑制CaM、CaMKⅡ的过度激活,减少组织中iNOS的过多产生,减轻组织炎症损伤。     

不同浓度40%溴酰·噻虫嗪SC灌根处理对苹果绵蚜防控效果评价

为明确不同浓度40%溴酰·噻虫嗪SC对苹果绵蚜的田间防效及苹果品质的影响,2018年在云南昭阳区苹果主产区果园,采用以清水灌根为对照,40%溴酰·噻虫嗪SC 500倍液、750倍液、1000倍液在落花期至幼果形成期灌根处理进行防控苹果绵蚜效果评价。结果表明,3个浓度的40%溴酰·噻虫嗪SC溶液均可持续降低苹果绵蚜虫落数,增加虫口减退率,对苹果绵蚜均有较好较长的防效,其中750倍液40%溴酰·噻虫嗪SC对绵蚜防效最好,500倍液和1000倍液的防效次之;品质分析表明,40%溴酰·噻虫嗪SC处理可增加苹果可溶性固形物和可溶性蛋白含量,而可溶性糖、可滴定酸和维生素C含量显著降低。从降低农药施用量及保证苹果品质出发,生产中建议以750倍液、1000倍液40%溴酰·噻虫嗪SC防治苹果绵蚜为佳。     

动物饲料中转基因抗草甘膦大豆GTS40-3-2成分的检测

转基因抗草甘膦除草剂大豆GTS 40-3-2作为目前在最多国家和地区批准种植的转基因大豆,除榨取大豆油外,剩余的豆粕等副产品被用作动物饲料。通过对山西省太谷周边11个动物养殖户的饲料进行转基因标识情况专项调查,采用定性和定量PCR技术对转基因抗草甘膦除草剂大豆GTS 40-3-2含有的Ca MV35S启动子、NOS终止子、Cp4-EPSPS及大豆内标Lectin基因进行检测。结果表明:11份饲料全部含有转基因抗草甘膦除草剂大豆GTS 40-3-2成分,且其含量各不相同,其中蛋鸡饲料GTS 40-3-2转化体的含量最低,但所有饲料均无转基因标识。试验为我国转基因饲料饲用安全性评价体系提供了试验数据支持,同时也对来源于转基因作物的原材料产品的合理应用以及通过转基因饲料喂养动物所产生的农畜产品的安全性评价具有重要的现实和理论意义。     

Gamma‐ray spectrometry as versatile tool in soil science: A critical review

Gamma‐ray spectrometry is an established method in geo‐sciences. This article gives an overview on fundamentals of gamma‐ray spectrometry that are relevant to soil science including basic technical aspects, and discusses influencing factors, inconsistencies, limitations, and open questions related to the method. Gamma‐ray spectrometry relies on counting gamma quanta during radionuclide decay of ⁴⁰K, ²³⁸U, and ²³²Th, but secular equilibrium for the decay series of U and Th must be given as decays of their respective daughter radionuclides are used for determination. Secular equilibrium for U and Th decay series, however, is not always given leading to, e.g., anomalies in U concentration measurements. For soil science, gamma‐ray spectrometry is of specific value since it does not only detect a signal from the landscape surface, but integrates information over a certain volume. Besides, different spatial scales can be covered using either ground‐based or airborne sensing techniques. Together with other remote sensing methods, gamma signatures can provide completive information for understanding land forming processes and soil properties distributions. At first, signals depend on bedrock composition. The signals are in second order altered by weathering processes leading to more interpretation opportunities and challenges. Due to their physico‐chemical properties, radionuclides behave differently in soils and their properties can be distinguished via the resulting signatures. Hence, gamma signatures of soils are specific for local environments. Processes like soil erosion can superimpose gamma signals from in situ weathering. Soil mappings, available K and texture determination, or peat and soil erosion mapping are possible applications being discussed in this review.     

扑·乙乳油防除大豆田杂草田间药效试验

采用田间小区试验方法,进行了质量分数为40%扑·乙乳油播后苗前土壤封闭处理防除大豆田杂草田间药效试验。结果表明:质量分数为40%扑·乙乳油对大豆田杂草有较好的防除效果,对单、双子叶杂草均有较高防效,45d杂草总防效为81 21%～88 34%,鲜重抑制效果为80 29%～88 21%。随使用剂量增加,药效逐渐提高,以400g 667m2防效最好,而且对大豆出苗及生长发育无不良影响,增产幅度为10 85%～14 55%。     

Radiological risk assessment of fish feed and feed raw materials

In this study, natural radioactivity concentrations of fish feed and feed raw materials were assessed. Several feeds and raw materials were provided from the suppliers who dominate the aquaculture sector. ²²⁶Ra, ²³²Th and ⁴⁰K concentrations were determined by using a high‐purity germanium detector. The measured activity concentrations of samples ranged from 3.28 ± 0.27 to 15.90 ± 1.36, from 1.27 ± 0.10 to 12.07 ± 1.21 and from 52.01 ± 2.60 to 1,158.96 ± 54.42 Bq/kg for ²²⁶Ra, ²³²Th and ⁴⁰K respectively. The gamma dose rate and annual effective dose rate of samples were calculated to be in the range of 1.99–50.47 nGy/h and 2.44–61.89 µSv/yr respectively. Since the calculated radiological risk parameters of the samples were lower than the world wide average values, the radiation hazard is insignificant for human handlers.     

会计电算化与ERP系统

国际著名的ERP产品是在90年代初进入中国，其推广应用水平还处在初级阶段。本文讨论了企业创建ERP的意义和基础要求，并对我国大多数企业而言建设ERP系统的条件作了较为全面的分析。ERP适用于大规模和超大规模的企业集团，做到精细化管理和规范化运作，它提供的是一种方法、手段和平台，并不是包治百病的灵丹妙药。对于大多数的中小企业而言，学习和引进ERP的思想，加快企业信息管理的现代化建设，尤其是会计电算化系统的建设，才是提高企业的管理水平、提升企业竞争力的当务之急。     

甘蓝白化种荚基因alb1的遗传与转录组分析

采用石蜡切片技术研究甘蓝白化种荚材料alb1的细胞学特征,结果表明白化种荚细胞形态发育异常,细胞壁畸形、细胞界限不明显。遗传分析结果表明,绿荚∶白荚的分离比为3∶1,白荚性状是由1对隐性基因控制的。利用转录组测序技术分析白化种荚突变体alb1及绿荚芥蓝TO1000的基因表达情况,筛选获得4 530个差异表达基因,其中2 569个基因上调表达,1 961个基因下调表达;这些基因主要涉及核糖体、植物激素信号转导、脂肪酸链伸长等生物学过程,植物激素信号转导对细胞形态建成尤为重要,可能是引起细胞发育畸形,出现白化种荚的重要原因。