Cloning and sequence analysis of nine novel MYB genes in Taxodiaceae plants

Myeloblastosis(MYB) is one of the largest transcribed factor families in plants. To gain an overall picture of the evolution of MYB genes in relict plants, we cloned nine novel MYB genes in Taxodiaceae plants(Taxodium distichum, Taxodium ascendens, Cryptomeria japonica var. Sinensis, Cryptomeria japonica cv. Araucarioides, Cryptomer Japonica, Metasequoia glyptostroboides, Cunninghamia lanceolata, Taiwania cryptomerioides and Glyptostrobus pensilis). The deduced amino acid sequences for MYBs showed that the nine MYB proteins contained two DNA binding domains. The first domain is from amino acid position 29 to 78, wherein three tryptophanes at 33, 53 and 73 were separated by 19 amino acids, respectively. The second domain is from amino acid position 82 to 127, wherein three tryptophanes at 86, 105 and 124 were separated by 18 amino acids, respectively, whereas the first tryptophane at amino acid position 86 is replaced by a phenylalanine. The characterization of these conserved domains at nine MYBs indicated that they all belong to the R2R3-MYB group. The secondary structure analysis showed that α-helix and β-turn are the major motifs of the predicted secondary structure of MYBs. The three dimensional model of each MYB protein showed that the structure is like clip, making it more flexible and mobile. The similarities between the nine MYB proteins in Taxodiaceae were calculated. The highest identical value of 99% is between CjsMYB, CjMYB and CjaMYB, whereas the lowest value of 82% is between TaMYB and ClMYB. According to the phylogenetic tree, the distances between different genera were relatively large whereas those within genera were relatively small. As expected, accessions of the same genus formed a subgroup before being grouped with other genera.     

Spatial heterogeneity of factors influencing forest fires size in northern Mexico

In Mexico, forest fires are strongly influenced by environmental, topographic, and anthropogenic factors. A government-based database covering the period 2000-2011 was used to analyze the spatial heterogeneity of the factors influencing forest fire size in the state of Durango, Mexico. Ordinary least squares and geographically weighted regression models were fit to identify the main factors as well as their spatial influence on fire size. Results indicate that fire size is greatly affected by distance to roads, distance to towns, precipitation, temperature, and a population gravity index. The geographically weighted model was better than the ordinary least squares model. The improvement of the former is due to the influence of factors that were found to be non-stationary. These results suggest that geographic location determines the influence of a factor on fire size. While the models can be greatly improved with additional information, the study suggests the need to adopt fire management policies to more efficiently reduce the effect of anthropogenic factors. These policies may include more training for landowners who use fire for clearing, closure of roads, application of thinning, prescribed burning, and fire breaks in perimeters adjacent to roads.     

基于全站仪精准量测树木材积方法初探

笔者根据北京林业大学冯仲科教授提出的精准林业的理念,使用全站仪对树木材积进行量测,并将全站仪量测的材积数据与解析法所得砍伐数据进行比较,结果表明,全站仪量测立木材积的精度在91%以上,平均精度在96%左右,完全能满足高精度量测立木材积的要求,建议推广应用。     

广西甘蔗主产区二点螟对防治农药的敏感性测定

[目的]通过抗药性监测,摸清广西主要蔗区二点螟对防治农药的敏感性,以期为广西甘蔗二点螟的合理用药及抗性治理提供参考.[方法]采用点滴法,测定广西主要蔗区二点螟种群对当前主要防治农药克百威、特丁磷和毒死蜱及新推广农药氯虫苯甲酰胺的敏感性.[结果]甘蔗二点螟对不同农药的敏感性由强到弱依次为:氯虫苯甲酰胺＞克百威＞特丁磷＞毒死蜱.不同蔗区二点螟种群对同一种农药的敏感性存在明显差异,克百威以南宁种群的耐药性最强,特丁磷以来宾种群的耐药性最强,毒死蜱以百色种群的耐药性最强,氯虫苯甲酰胺对各地二点螟种群仍保持高度敏感,其致死中量为0.4177～0.6087μg/g.不同蔗区二点螟种群对农药反应的分散程度由大到小依次为:氯虫苯甲酰胺＞克百威＞毒死蜱＞特丁磷.[结论]甘蔗二点螟对克百威、毒死蜱和特丁磷已产生不同程度的耐药性,对氯虫苯甲酰胺仍然保持高度敏感,但二点螟对氯虫苯甲酰胺产生抗药性的风险相对较高.     

大豆高产栽培技术

<正>一、选地宜选择土壤比较肥沃,保水性好,通气性强,两年未种过大豆的地块。杜绝重茬,避免迎茬,上茬作物施用过阿特拉津的地块最好隔一年种豆。二、精细整地1.整地最好是秋整地。秋季,前茬收获后,用除茬机将茬子打碎,进行秋翻,耕翻深度16~20厘米,接着进行耙地、镇压。耕、耙、压作业后,地表要平整,土壤要细碎,耕层要上松下实,无大土块和暗坷垃。2.施基肥春耙前,亩施腐熟过的有机肥2000公斤、磷酸二铵10     

二代棉铃虫、甜菜夜蛾药剂防治试验报告

<正>一、试验目的2014年6~7月份,西华县二代棉铃虫、甜菜夜蛾大发生。夏玉米(苗)、花生、大豆田虫量多,危害重,群众反映防治难度较大。为了有效指导生产防治,笔者选用了几种市场上推广应用的杀虫剂进行喷药实验,以验证其杀虫效果及应用价值。二、供试作物夏大豆4~6复叶期三、防治对象二代棉铃虫、甜菜夜蛾四、供试药剂1.连战(20%虫酰肼悬浮剂)山东中新科农业生物科技有限公司;2.用5%田歌乳油,山东天润化工有限公司推     

基于1stOpt软件的二元立木材积方程的研究

选取旺业甸实验林场201株落叶松样木胸径、树高、材积精测数据,利用1stOpt优化分析计算软件平台的Levenberg-Marquarat+通用全局优化算法(LM-UGO)拟合二元材积方程,并与前人研究得到较好结果的遗传算法(GA)和加权最小二乘法(WLS)进行比较。结果表明,LM-UGO、GA和WLS均可得到较高精度的二元材积方程,LM-UGO方法能够比GA和WLS法更好地拟合二元材积方程,且具有操作方便、拟合快速而准确、收敛速度快等优点,是拟合二元立木材积方程的有力工具。经F检验,三种方法的F值LM-UGO相似文献   

稿件要求

<正>《现代园林》杂志由中国农业大学观赏园艺与园林系、北京中绿园林科学研究院承办,已被CNKI中国期刊网全文数据库等收录。一、质量要求本刊定位中级技术性期刊,主要刊登具有一定创新性的研究和设计论文。二、内容要求1题目(中英文)25字以内。开门见山,不加副标题,中英文对应。2作者姓名中文,及汉语拼音(姓前名后)。3单位全称(与公章一致),城市邮政编码,中英文对照。     

污水处理工程经济费用模型分析与应用

指出了合理的污水处理厂经济费用估算是确定项目投资的基础,能成为项目资金筹措及制订建设贷款计划的有效依据。基于市政排水工程投资估算指标数据,构建了城镇污水及再生水治理投资建设和运行费用函数的模型。运用线性回归的方法对不同规模的污水处理厂和再生水深度处理的建设费用进行了分析,确定了经济费用估算模型的建设费用函数和运行费用的参数。最终结合实例对提出的费用估算模型的准确性进行了分析。结果表明：建立的污水处理厂经济费用估算模型与实际费用误差较小,满足工程费用的估算要求。     

阜新市辖区土壤速效养分的时空变化及分析

<正>阜新市位于辽宁省西北部,境内有五个辖区:海州区、太平区、新邱区、细河区、清河门区,包括6个乡镇54个行政村,全区大部分被阜新蒙古族自治县包围,总耕地面积120km2。一、土壤碱解氮1.耕层土壤碱解氮含量现状分析全区土壤碱解氮含量的变化范围为46.83~116.18mg/kg,平均为79.21mg/kg。土壤碱解氮分级及面积统计见表1。从表中可以看出,处于中等的三级水平的耕地占耕地总面积22.20%;较缺