Expression relevance of GATA-1 and miR-451a in erythroid differentiation of K562 cells under hypoxia

AIM To investigate the expression relevance of GATA binding protein-1 (GATA-1) and microR?NA-451a (miR-451a) in erythroid differentiation of human chronic myeloid leukemia K562 cells under hypoxia. METHODS The K562 cells were divided into 2 groups: normoxia group and hypoxia (1% O₂) group, and 40 μmol/L hemin chloride was used to induce K562 cell differentiation for 48 and 72 h. The mRNA expression of γ-globin was detected by RT-qPCR, hemoglobin production was observed by benzidine staining, and flow cytometry was used to detect CD235a expression for verifying erythroid differentiation model. The protein expression of GATA-1 during K562 cell differentiation under normoxia and hypoxia was determined by Western blot. RT-qPCR was used to detect the mRNA expression of GATA-1 and the expression level of miR-451a, and their correlation was analysis. The K562 cells were infected by lentivirus for over-expression or knock-down of GATA-1. Meanwhile, the morphological changes of the cells in the above groups were analyzed by Wright-Giemsa staining method to clarify the erythroid differentiation of K562 cells. The expression miR-451a was detected by RT-qPCR after GATA-1 over-expression or knock-down. REULTS: Under normoxia and hypoxia conditions, the expression levels of γ?-globin and CD235a and the positive rate of benzidine staining at 48 and 72 h were significantly higher than those at 0 h (P<0.05).At 72 h, the expression levels of γ?-globin and CD235a and the benzidine staining positive rate in hypoxia group were significantly higher than normoxia group (P<0.05). The expression of GATA-1 mRNA and miR-451a under hypoxia showed an upward trend during the erythroid differentiation of K562 cells, and was significantly higher than that in normoxia group at 72 h (P<0.05). Correlation analysis showed that the mRNA expression of GATA-1 was positively correlated with miR-451a expression under hypoxia (P<0.01). After over-expression of GATA-1 under hypoxia, the expression of γ-globin and CD235a, the positive rate of benzidine staining, and the cell counts of size augmentation, nuclear deflection and nuclear shrinkage at 72 h were significantly higher than those in negative control group (P<0.05). After knock-down of GATA-1 under hypoxia, the expression of γ-globin and CD235a, the benzidine staining positive rate, and the cell counts of size augmentation, nuclear deflection and nuclear shrinkage at 72 h were significantly lower than those in negative control group (P<0.05). Compared with negative control group under hypoxia, the expression of miR-451a was significantly increased after GATA-1 over-expression (P<0.05), while the expression of miR-451a was significantly decreased after GATA-1 knock-down (P<0.05). CONCLUSION Hypoxia increases the expression of GATA-1 and then up-regulates miR-451a to promote erythroid differentiation of K562 cells.     

基于层次分析模型的西宁地区雷电灾害风险区划

分析2008—2017年西宁地区雷电灾害历史数据,2008—2017年闪电定位数据和2017年《西宁市统计年鉴》数据;选取闪电强度、闪电密度、人工雷暴日数、土壤电导率、河网密度、人口密度、GDP密度、生命易损指数、经济易损指数、防护能力指数等10个因子作为西宁地区雷电灾害风险评估指标,结合层次分析法采用致灾因子危险性、承灾体暴露度以及承灾体脆弱性反映西宁地区雷电灾害风险,实现了西宁地区雷电灾害风险区划。结果表明：雷电灾害高风险区出现于大通县东北部、西北部的宝库乡,湟中县与西宁市交界处由北至南的带状地区;中风险区主要出现于西宁主城区、湟中县大部分乡镇、大通县东北部乡镇;低风险区分布分散,主要出现在湟源县西南部乡镇、大通县中部乡镇。     

Comparison of Blood Physiological and Biological Indexes of the Polled Yak and Horned Yak

The blood physiological and biological indexes were determined in both polled and horned yaks, to investigate the differences of body condition and metabolism between the two breeds, and provide a comprehensive understanding for new yak breeds. Thirty physiological and biochemical indexes of the polled and horned yaks were measured, and all these data were analyzed by independent-samples t test to compare the same index between the two groups. The results showed that there were extremely significant differences (P<0.01) in 11 indexes,5 indexes reached significant differences (P<0.05) and no significant differences (P>0.05) in 14 indexes between the polled and horned yaks. The results suggested that the polled yak was easier to adapt to the plateau hypoxia and there were many differences in adaptability of plateau hypoxia, kidney metabolism, liver metabolism and the growth and development of the bone between the polled and horned yak. This study could provide scientific basis for better protection and utilization of yak and the selection of the new yak breeds.     

青海省农作物品种选育及品种志编撰

概述了改革开放30年来,青海省农作物育种工作的主要成就,育种目标和育种技术的演变特征;详细叙述了《青海省农作物品种志》(1983-2005)编撰的技术路线及图像处理、电子系统开发、资料采集等关键技术和思路,为更好了解和使用《青海省农作物品种志》提供参考。     

青海省高家湾滑坡的形成条件分析及稳定性评价

[目的]评价青海省高家湾滑坡稳定性,为其灾害防治提供一定指导。[方法]在对高家湾滑坡基本特征分析基础上,先开展其形成条件研究;其次,利用传递系数法和瑞典圆弧法实现其稳定性现状评价,并进一步采用长短时记忆神经网络构建其变形预测模型,实现其稳定性预测评价。结合稳定现状评价结果,实现其稳定性的综合研究。[结果]高家湾滑坡具有滑坡形成的基本条件,且具有复合多期巨型规模特征,对其防治应具有综合性;同时,滑坡现状处于稳定状态,但局部次级滑坡存在失稳可能,且在不利工况条件下,稳定性将会进一步减弱;另外,据变形预测研究结果,得出其平均相对误差均小于2%,进而验证了本文优化LSTM模型的有效性,且通过外推预测,得出高家湾滑坡变形仍将继续增加,且无收敛迹象。[结论]高家湾滑坡稳定性还会进一步减弱,趋于不利方向发展,应尽快采取必要措施切实保证滑坡稳定。     

青海孟达国家级自然保护区野生种子植物区系分析

对青海孟达国家级自然保护区野生种子植物进行了野外实地调查,同时,对相关文献进行了查询和分析,结果表明,孟达国家级自然保护区野生种子植物共计有79科325属664种。其中,温带分布的科占据优势,其次为世界分布和热带分布。属的分布上,温带分布占据绝对优势,世界分布、热带分布、东亚分布和中国特有分布为辅,并有极少其他分布类型。同时,与青海植物区系进行比较,显示两者存在明显差异。系统的分析结果显示：该地区有明显的边缘分布特征;植物的垂直分布呈现一定的规律性;该地区植物资源丰富,国家珍稀物种及有经济价值、药用价值的植物众多,观赏植物更为广布。     

中药羌活的价值研究

本文主要对中药羌活的化学成分、药理研究及临床应用等方面的研究概况进行了综述,并对其研究方向进行了展望,为中药羌活资源的进一步开发利用提供参考和依据。     

四翅滨藜生物脱盐调查初报

通过检测,四翅滨藜林下土壤60cm土层内,每立方米土壤含盐量比对照减少2.81kg,其植物灰分中,全盐量的比重高达43.8%。这一结果表明,四翅滨藜能够吸纳土壤中的盐分,改良盐碱地作用明显。     

ICP-MS法测定沙棘果油中13种微量元素含量

沙棘果油样品经微波消解处理,采用电感耦合等离子体质谱法测定所得样品溶液中铁、硒、锌、钾、钠、钙、镁、铜、锰、铅、砷、汞、镉等13种微量元素的含量。使用动态反应池技术消除了多原子离子对部分被测元素的干扰。方法快速准确、灵敏度高,加标回收率在96.1%～101%之间,相对标准偏差小于3%。     

重金属Cr6+、Mn2+污染对小麦幼苗生理生化特性及生长的影响研究

对不同浓度Cr6+、Mn2+处理的小麦种子萌发、小麦叶片叶绿素组成含量及SOD、POD酶活性的变化进行了研究。结果表明,低浓度处理(Cr6+浓度≤10 mg/L、Mn2+浓度≤8 mg/L)能够促进小麦种子萌发和生长,提高叶绿素含量及SOD、POD酶活性;但随着处理浓度的升高,Cr6+、Mn2+对小麦的抑制作用逐渐增强,毒害作用明显。