草浆造纸木质素对土壤性质和小白菜生长的影响

草浆造纸废液是影响秸秆造纸发展的重要因素,利用小白菜盆栽试验研究了施用麦秸造纸废液木质素和麦秸对土壤性质及小白菜生长的影响,以期为造纸废弃物的资源化利用提供依据。结果表明,两茬小白菜收获后,铵木素配施N肥使土壤pH值降低;小白菜两茬连作后铵木质素配施的土壤活性有机碳含量及多酚氧化酶活性分别为2.73g·kg-1和1.77mg·g-1·2h-1,显著高于其他处理;除对照和单施氮肥外,两茬盆栽收获后土壤多酚氧化酶活性均高于第一茬试验。两茬小白菜收获后秸秆配施和碱木质素配施氮肥土壤过氧化物酶活性均高于其他处理。铵木质素配施及秸秆配施的小白菜干重相近,显著高于碱木质素配施。研究表明,与秸秆还田相比,施用铵木质素没有对土壤性状和小白菜生长产生不利影响,故铵法草浆木质素可以作为一种潜在的土壤有机改良剂应用。     

Effects of Zinc Deficiency and Drought on Plant Growth and Metabolism of Reactive Oxygen Species in Maize （Zea mays L.）

The combinative effects of applied zinc (Zn) and soil moisture on the plant growth, Zn uptake, and the metabolism of reactive oxygen species (ROS) in maize (Zea mays L.) plants were examined through two pot experiments under greenhouse conditions. Maize variety Zhongdan 9409 was used. In experiment 1, maize plants were grown in cumulic cinnamon soil with five Zn treatments (0, 3.0, 9.0, 27.0, and 81.0 mg Zn kg-1 soil). Three treatments of soil moisture including serious drought, mild drought, and adequate water supply were set at 30-35 %, 40-45 %, and 70-75 % (w/w) of soil saturated water content, respectively. Soil saturated water content was 36% (w/w). The dry matter weights of shoots were enhanced by Zn application and adequate water supply. There was no apparent difference in plant growth among Zn application rates from 3.0 to 81.0 mg Zn kg-1 soil. The increases of plant growth and Zn uptake due to Zn application were found more significant under well-watered condition than under drying condition. In experiment 2, two levels of Zn (0 and 5.0 mg Zn kg-1 soil) and soil moisture regimen (40-45 % and 70-75 % of soil saturated water content, respectively) were set. Zn deficiency or water stress resulted in higher concentrations of O2-· and malondiadehyde in the first fully expanded leaves.Zn deficiency lowered the activity of superoxide dismutase (SOD, EC 1.15.1.1) in leaves. Drought stress increased SOD activity in leaves regardless of Zn supply. The activity of guaiacol peroxidase (POD, EC1.11.1.11) was found to be enhanced by Zn supply only in well-watered leaves. Zinc deficiency or water stress had little effect on the activity of catalase (CAT, EC 1.11.1.6). The higher ROS level in early maize leaves due to water stress seemed not to be alleviated or lowered partially by Zn application. However, Zn fertilizer was recommended to apply to maize plants irrigated or supplied with adequate water, otherwise Zn deficiency would reduce the water use for plant biomass production.     

Effects of different management practices on the soil–water balance and crop yield for improved dryland farming in the Chinese Loess Plateau

Field experiments were carried out to study the effects of different soil management practices on the water balance, precipitation use efficiency (PUE), and crop yield (i.e. winter wheat and peanut) on a loess soil near Luoyang (east edge of the Chinese Loess Plateau, Henan Province, China). Field plots were set up in 1999 including following soil management practices: subsoiling with mulch (SS), no-till with mulch (NT), reduced tillage (RT), two crops per year (i.e. winter wheat and peanut, TC), and a conventional tillage control (CT). The field plots were equipped to monitor all components of the soil–water balance except evapotranspiration, which was computed by solving the water balance equation. The results showed that although soil management had smaller influence on the magnitude of the water balance components than did precipitation variations, small influences of the applied soil management practices on water conservation during the fallow period can greatly affect winter wheat yield. SS increased consistently precipitation storage efficiency (PSE) and PUE over the 5 years compared to CT except during the wettest year. NT also had a noticeable effect on postharvest water storage during the fallow period; however, the influence on yield of NT depended on the amount of precipitation. TC lowered the winter wheat yield mainly due to the unfavorable soil moisture conditions after growing peanut in summer; however, the harvested peanut gained an extra profit for the local farmer. No matter which kind of soil management practices was adapted, PSE never exceeded 41.6%, which was primarily attributed to high evapotranspiration. From data of five consecutive agricultural years between 2000 and 2005, it could be concluded that SS resulted in the highest PSE, PUE and crop yield. TC also showed promising results considering the economic value of the second crop. NT performed slightly less as SS. CT gave intermediate results, whereas RT was the worst alternative.     

培养基质对丛枝菌根(AM)真菌生长发育的影响

温室条件下，以盆栽培养方法研究不同培养基质对丛枝菌根(Arbuscular Mycorrhizae,AM)真菌Glomus mosseae生长发育的影响。结果表明：不同理化性质的基质对菌根共生体生长发育的影响不同，综合考虑菌根长度、根外菌丝量及孢子数3项指标,以沙土混合物(体积比3∶1)对G. mosseae菌剂的生长发育最为有利。宿主植物菌根长度及根中的可溶性糖浓度与根外孢子数有正相关关系，而宿主植物中磷浓度与菌根真菌的生长发育也有类似的关系。说明培养基质的养分状况、水分状况、通气状况等诸多因素都会影响菌根共生体的建立和发展。宿主植物的菌根长度、根中可溶性糖浓度以及宿主植物磷浓度对菌根真菌的生长发育有显著影响。因此，工厂化AM菌剂生产中，应以沙土混合物(体积比3∶1)为生产G. mosseae菌剂的培养基质。     

耕作方式转变和秸秆还田对土壤活性有机碳的影响

深松是解决长期旋免耕后耕层浅薄化、亚表层(15~30 cm)容重增加等问题的有效方法之一,长期旋免耕后进行深松显著影响土壤有机碳及其组分的周转。为对比转变耕作方式对土壤活性有机碳(LOC)及碳库管理指数的影响,该研究基于连续6 a的旋耕转变为深松和免耕转变为深松定位试验,对比了2012-2014年长期旋免耕农田进行深松对农田土壤活性有机碳及碳库管理指数的影响。研究结果表明,耕作方式转变和秸秆还田均对土壤LOC含量、活性有机碳与有机碳的比例(LOC/SOC)和碳库管理指数产生显著影响。相对于原旋耕秸秆还田处理(RTS),虽然旋耕-深松秸秆还田处理(RTS-STS)提高了0~30 cm土层的LOC含量,但其土壤中LOC/SOC比例和碳库管理指数显著下降。而免耕-深松秸秆还田(NTS-STS)处理和耕作方式未转变的免耕秸秆还田处理(NTS)在0~10 cm土层其LOC含量无显著性差异,但NTS-STS处理显著提高LOC/SOC比例。耕作方式转变导致RTS-STS处理碳库管理指数随着土层的加深而逐渐降低,而NTS-STS处理则呈逐渐升高趋势。耕作、秸秆、年份、耕作与秸秆、耕作与年份及3者交互作用是导致耕作方式转变后各处理0~30 cm的LOC含量变化的主要作用力(P0.05)。秸秆还田条件下,将长期旋耕处理转变为深松可显著降低土壤SOC中的LOC比例,降低碳库管理指数,促进土壤碳库的稳定性;而长期免耕处理转变为深松能够显著提高土壤下层(10~30 cm)的土壤碳库活性。     

Arbuscular mycorrhizal fungi optimize the acquisition and translocation of Cd and P by cucumber (<Emphasis Type="Italic">Cucumis sativus</Emphasis> L.) plant cultivated on a Cd-contaminated soil

Purpose

Fruiting vegetables are generally considered to be safer than other vegetables for planting on cadmium (Cd)-contaminated farms. However, the risk of transferring Cd that has accumulated in the stems and leaves of fruiting vegetables is a major issue encountered with the usage of such non-edible parts. The objective of this study was to resolve the contribution of arbuscular mycorrhizal (AM) fungi to the production of low-Cd fruiting vegetables (focusing on the non-edible parts) on Cd-contaminated fields.

Materials and methods

An 8-week pot experiment was conducted to investigate the acquisition and translocation of Cd by cucumber (Cucumis sativus L.) plants on an unsterilized Cd-contaminated (1.6 mg kg^?1) soil in response to inoculation with the AM fungus, Funneliformis caledonium (Fc) or Glomus versiforme (Gv). Mycorrhizal colonization rates of cucumber roots were assessed. Dry biomass and Cd and phosphorus (P) concentrations in the cucumber shoots and roots were all measured. Soil pH, EC, total Cd, phytoavailable (DTPA-extractable) Cd, available P, and acid phosphatase activity were also tested.

Results and discussion

Both Fc and Gv significantly increased (P?<?0.05) root mycorrhizal colonization rates and P acquisition efficiencies, and thus the total P acquisition and biomass of cucumber plants, whereas only Fc significantly increased (P?<?0.05) soil acid phosphatase activity and the available P concentration. Both Fc and Gv significantly increased (P?<?0.05) root to shoot P translocation factors, inducing significantly higher (P?<?0.05) shoot P concentrations and shoot/root biomass ratios. In contrast, both Fc and Gv significantly decreased (P?<?0.05) root and shoot Cd concentrations, resulting in significantly increased (P?<?0.05) P/Cd concentration ratios, whereas only Gv significantly decreased (P?<?0.05) the root Cd acquisition efficiency and increased (P?<?0.05) the root to shoot Cd translocation factor. Additionally, AM fungi also tended to decrease soil total and phytoavailable Cd concentrations by elevating plant total Cd acquisition and soil pH, respectively.

Conclusions

Inoculation with AM fungi increased the P acquisition and biomass of cucumber plants, but decreased plant Cd concentrations by reducing the root Cd acquisition efficiency, and resulted in a tendency toward decreases in soil phytoavailable and total Cd concentrations via increases in soil pH and total Cd acquisition by cucumber plants, respectively. These results demonstrate the potential application of AM fungi for the production of fruiting vegetables with non-edible parts that contain low Cd levels on Cd-contaminated soils.

     

方正县土壤硒的分布特征及其与土壤性质的关系

方正县是黑龙江省水稻主产区,为研究方正县土壤全硒(Se)的含量、分布特征及其与土壤性质的关系,在哈尔滨市所属方正县境内采集127个0~20 cm耕层土壤样品,分析了方正县土壤全硒含量及其影响因素。结果表明,方正县土壤全硒含量变幅为0.030~0.496 mg kg-1,算术平均值为0.228 mg kg-1略低于全国土壤平均硒含量,总体上处于中等、边缘及缺乏水平。在不同类型土壤中,泥炭土硒含量相对较高(0.267 mg kg-1)而沼泽土硒含量相对较低(0.153 mg kg-1)。不同成土母质中,以坡积物为母质发育的土壤硒含量较高(0.276 mg kg-1)而以冲洪积物为母质发育的土壤硒含量较低(0.207 mg kg-1)。在土地利用方面,稻田土壤全硒含量略高于旱地土壤。相关分析表明:土壤有机碳(SOC)与土壤粒径分布(PSD)是影响研究区表土层土壤硒含量的主要因素。     

不同施氮水平下水稻的养分吸收、转运及土壤氮素平衡

【目的】为解决东北地区水稻合理施用氮肥问题,系统研究了不同施氮水平条件下,东北水稻产量及构成因素、养分吸收、转运、氮肥利用效率及土壤氮素平衡的变化,并探讨各养分间及其与产量间的关系,为东北地区水稻合理施氮提供理论基础。【方法】于2012~2013年在吉林省松原市前郭县红光农场,选用当地主栽水稻品种富优135和吉粳511为材料,设置施N 0、60、120、180和240 kg/hm25个水平。于水稻返青期、分蘖期、抽穗期、灌浆期及成熟期采集植株样本,分为茎鞘、叶片和籽粒三部分,测定氮、磷、钾含量,计算水稻主要生育期植株养分吸收、转运、氮素利用特性的相关参数及各养分吸收、转运与产量间的关系。水稻移栽前和收获后采集0—100 cm土壤样品,每20 cm为一层(共5层),测定铵态氮、硝态氮含量,并根据各层土壤容重计算0—100 cm土体无机氮积累量,分析土壤氮素平衡状况。【结果】施氮量60~180 kg/hm2范围内,水稻产量随着施氮水平的提高而增加,氮肥用量超过180 kg/hm2水稻产量下降。结合当年水稻和肥料价格,根据水稻产量(y)和施氮量(x)拟合方程,得出最高产量氮肥用量分别为212.8 kg/hm2和220.6 kg/hm2,施氮范围在202.2~231.6 kg/hm2之间,最佳经济产量氮肥用量分别为203.0和209.1 kg/hm2,施氮范围在192.9~219.6 kg/hm2之间。施用氮肥可显著提高水稻主要生育期氮、磷、钾吸收量,且能提高水稻抽穗期氮、磷、钾养分向籽粒的转运,施氮量180 kg/hm2处理抽穗期各养分累积量与籽粒转运量呈正比,当氮肥用量超过180 kg/hm2后,氮、磷、钾养分向籽粒转运出现负效应。氮素农学利用率和偏生产力随着施氮水平的提高而显著下降,氮肥当季回收率以施氮量180 kg/hm2处理最高。相关分析表明,水稻主要生育期氮、磷、钾的吸收、转运与产量间均存在显著或极显著的正相关性,其中灌浆期氮、磷、钾的吸收状况与产量间的相关系数最大。施用氮肥可显著提高收获后0—100 cm土壤中残留无机氮(Nmin),氮素表观损失量随施氮水平的提高而增加。【结论】适宜的氮肥用量可显著提高水稻产量,各生育时期养分吸收总量,提高水稻生育后期秸秆中氮、磷、钾向籽粒的转运量,并能降低土壤氮素表观损失量。综合考虑提高水稻产量、效益、氮肥当季回收率及维持土壤氮素平衡等因素,在本试验条件下,施氮范围在192.9~219.6 kg/hm2。     

Effects of mycorrhizal inoculation of upland rice on uptake kinetics of arsenate and arsenite

We assessed the effect of mycorrhizal inoculation on short‐term uptake kinetics of arsenate and arsenite by excised roots of upland rice (Oryza sativa L. cv. Zhonghan 221). A concentration of 0.01–0.05 mM arsenic (As) differentially affected the influx rates of both arsenate and arsenite into rice roots non‐inoculated or inoculated with Glomus mosseae and G. versiforme. While V_max for arsenate uptake by non‐mycorrhizal roots was 1.02 µmol g^?1 fresh weight h^?1, it was reduced by a factor of 2.4 for mycorrhizal roots (about 0.42 µmol g^?1 fresh weight h^?1) in the high‐affinity uptake system. However, at high concentrations of 0.5–2.5 mM As only G. versiforme was able to reduce As influx. The results show that mycorrhizal effects on As uptake of upland rice are both concentration and species‐specific.