植物内流型钾离子通道的研究进展*

 内流型钾离子通道是植物钾离子吸收的重要途径之一。近年来,已从多种植物或同种植物的不同组织器官中分离得到多个内流型钾离子通道基因（如AKT1, KAT1, SIRK和KST1等）。从内流型钾离子通道基因的分类、结构、生理功能及在植物的应用等4方面综述了关于植物内流型钾离子通道的研究进展。     

不同施肥量对甜荞主要产量指标的影响

通过对定西地区甜荞不同施肥量下株高、分枝数、产量、降水利用效率等指标的综合比较认为,最佳的施肥量为：施纯N 22.5kg/hm2、P2O5 30.46kg/hm2、K2O 65.48kg/hm2,其对应株高73.4cm,分枝数4.4个,产量896.18kg/hm2,降水利用效率5.38kg/(hm2·mm)。     

Agronomy of fibre hemp (Cannabis sativa L.) in Europe

Fibre hemp may yield up to 25 t above ground dry matter per hectare (20 t stem dry matter ha⁻¹) which may contain as much as 12 t ha⁻¹ cellulose, depending on environmental conditions and agronomy. Its performance is affected by the onset of flowering and seed development. Effects of cultivar and management on yield and quality were tested at three contrasting sites in Italy, the Netherlands and the UK in three years, making use of standardised protocols for experimental design and research methodology. Highest yields (up to 22.5 t dry matter ha⁻¹) were obtained in Italy when later cultivars were used. Attainable yields proved slightly lower in the Netherlands and much lower in the UK. The quality of the cellulose was relatively stable over the growing season, but lignification may proceed rapidly some time after flowering. Crop development was very rapid and crops maintained green leaf area for a long time, thus radiation interception was considerable. The radiation use efficiency changed during development. It was lower after flowering (about 1.0 g MJ⁻¹ PAR) than before (about 2.2 g MJ⁻¹ PAR). Growing earlier cultivars to obtain some seed set advanced the reduction in radiation use efficiency. Nitrogen proved to affect yield only slightly. A relatively small amount of fertiliser will be adequate to cover the crop’s needs. Plant density declined during growth in a site-specific manner when it was high initially. Very low plant densities may not show this self-thinning but reduced yield and (especially) quality. Final plant densities were proven to depend more on initial plant stands than expected from literature. This was true at all three contrasting sites and in the different years. Nitrogen and plant density hardly interacted within one site. Results suggest that hemp can yield large quantities of useful cellulose when ecologically adapted cultivars are sown in proper plant densities. The cultivation is environmentally friendly with little harmful accumulation or emission of chemical inputs. More research on ideotyping is required and breeding efforts should be broadened.     

Polymerisation of gluten proteins in developing wheat grain as affected by desiccation

The breadmaking quality of wheat is affected by the composition of gluten proteins and the polymerisation of subunits that are synthesised and accumulated in developing wheat grain. The biological mechanisms and time course of these events during grain development are documented, but not widely confirmed. Therefore, the aim of this study was to monitor the accumulation of gluten protein subunits and the size distribution of protein aggregates during grain development. The effect of desiccation on the polymerisation of gluten proteins and the functional properties of gluten were also studied. The results showed that the size of glutenin polymers remained consistently low until yellow ripeness (YR), while it increased during grain desiccation after YR. Hence, this polymerisation process was presumed to be initiated by desiccation. A similar polymerisation event was also observed when premature grains were dried artificially. The composition of gluten proteins, the ratios of glutenin to gliadin and high molecular weight-glutenin subunits to low molecular weight-glutenin subunits, in premature grain after artificial desiccation showed close association with the size of glutenin polymers in artificially dried grain. Functional properties of gluten in these samples were also associated with polymer size after artificial desiccation.     

Long-term management impacts on soil C,N and physical fertility: Part III: Tamworth crop rotation experiment

Degradation of soil structure can lead to increased risk of run-off and soil erosion, and therefore, it is necessary to implement management practices that are more sustainable and will enhance and rehabilitate soils while increasing food production. The impact of small-grain rotations grown with legumes, fallow and continuously on total C (C_T), labile C (C_L), non-labile C (C_NL), total N (N_T), aggregation expressed as mean weight diameter (MWD) and infiltration determined as unsaturated hydraulic conductivity (K_unsat) were examined in a long-term rotation trial established in 1966 on a Black Earth (Pellic Vertisol) and a Red Clay (Chromic Vertisol) soil near Tamworth, in New South Wales, Australia. The results were compared with an adjacent uncropped pasture on each soil type. Cropping reduced all C fractions, N_T, MWD and K_unsat on both soils, which were further degraded when long fallowing was included in the rotation. C_L decreased by 70% with long fallow in the Red Clay and by 78% in the Black Earth compared with the adjacent pasture, while MWD decreased by 61% in the Red Clay and 91% in the Black Earth. Rotation of cereals with legumes resulted in smaller decreases in C fractions, N_T, MWD and K_unsat when compared with pasture. Rotation with lucerne (Medicago sativa) resulted in 41% higher C_L, 45% higher MWD and 87% higher K_unsat (10 mm tension) than long fallow on the Red Clay soil and 65, 126 and 43% higher on the Black Earth soil. There were strong positive correlations of soil C fractions and N_T with MWD for both soil types. Similar significant relationships were found for all C fractions and N_T with K_unsat (10 mm tension) for the Red Clay soil, but not for the Black Earth. Rotations with forage legumes can limit declines in C fractions, N_T, MWD and K_unsat when cropping these soils and has potential to increase soil sustainability.     

Population dynamics of arthropods in a sunn-hemp zucchini interplanting system

Zucchini, Cucurbita pepo L., is often colonized by economically important insect pests such as the striped (Acalymma vittatum Fab.) and spotted (Diabrotica undecimpunctata howardi Barber) cucumber beetles. To evaluate the impact of an interplanted cover crop on arthropods associated with zucchini, field experiments were conducted in which sunn hemp (Crotalaria juncea L.) was interplanted with zucchini as a living mulch and compared with zucchini monoculture (bare-ground) during 2009, 2010 and 2011 growing seasons. The experiment consisted also of two types of fertilizer usage including the application of synthetic or organic fertilizer in the form of chicken manure. Foliar counts of arthropods conducted on zucchini plants showed significantly lower numbers of the striped cucumber beetle in sunn hemp interplanted plots compared to bare-ground treatment plots. Also, fewer spotted cucumber beetles were found on zucchini plants in sunn hemp plots. Aphid abundances were variable during the study and significantly lower in sunn hemp treatment plots at one study site in 2009. Among predators, spiders were significantly more abundant in sunn hemp treatment plots during 2009. Fertilizer type did not have a significant effect on arthropod numbers on zucchini plants. Potential causes of arthropod population differences among the two treatments are discussed.     

Salicylate and biomass yield,and leaf phenolics of dark-leaved willow (Salix myrsinifolia Salisb.) clones under different cultivation methods after the second cultivation cycle

Willow based herbal products containing salicin and its derivatives can be used to alleviate aches and fevers. To guarantee the high quality of the willow drugs and moderate low cultivation and transportation costs, the willows with high salicylate concentrations are recommended for herbal production. Dark-leaved willow (Salix myrsinifolia Salisb.) with its high salicylate content is a promising source of herbal drugs. In the present study, seven dark-leaved willow clones were cultivated under short-rotation system with a two-year cultivation cycle. The yields of salicylates and biomass, and the concentrations of foliar phenolics were determined after the second cultivation cycle. Willow clones were treated by different cultivation methods, i.e. combinations of mulching (black polythene vs. cutter chip) and fertilisation (0, 25, 150 kg ha^?1, N), with the objective of evaluating the most productive clone and cultivation method for herbal willow production. Furthermore, we compared our results to those obtained after the first cultivation cycle. Results showed that the yields of salicylates and biomass, and the concentrations of all foliar phenolic metabolites varied significantly amongst the clones. Biomass yields and salicylate yield were increased by polythene mulching, but the level of response varied between clones. Fertilisation (only the highest rate) caused an increase in yields, but the effect of fertilisation was smaller than that of mulching. Salicylate yield of most clones was higher than that had been during the previous rotation. Phenolic analyses showed that the concentrations of chlorogenic acid, total p-OH-cinnamic acids and soluble condensed tannins were higher in the willows grown in polythene mulch plots than in willows grown on cutter chip mulched plots. Furthermore, the concentrations of diglucoside of SaOH, salicin, disalicortin 1, disalicortin 2, chlorogenic acid, total chlorogenic acids and total flavonoids were higher in fertilized than in unfertilized willows. Salicortin, which was the most abundant salicylate, and total salicylates were not affected by fertilisation and mulching. The effects of fertilisation and mulching on several phenolics were clone-dependent. In conclusion, after two cultivation cycles, the cultivation methods were not effective in increasing the foliar total salicylate concentration, but rather increased the salicylate yield by enhancing the biomass of the synthesising tissues. Since biomass production was increased by the second cultivation cycle, the cultivation of herbal willows under a two-year cycle is recommended. The highly variable responses of the clones to treatments indicate that profitable willow cultivation for herbal industry is based on the right clone selection.     

Effect of Brassicaceae seed meals with different glucosinolate profiles on Rhizoctonia root rot in wheat

The soil-borne pathogen Rhizoctonia solani AG 8 causes major yield losses in wheat (Triticum aestivum. L) production worldwide. Plant tissues of Brassicaceae species contain glucosinolates that are hydrolyzed in the presence of the enzyme myrosinase into products with pesticidal properties. Growth chamber studies were conducted to determine the effect of the Brassicaceae seed meals (SMs) from Brassica juncea, Brassica napus and Sinapis alba on the suppression of the R. solani AG 8 infection of winter wheat. Pasteurized sandy soils were amended with intact and denatured SMs of rape seed and mustard at a rate of 0.5% by soil weight. Regardless of the glucosinolate type and content, all intact and denatured Brassicaceae significantly reduced the infection of winter wheat seedlings by R. solani AG 8 compared to the un-amended control. However, soils amended with S. alba SMs had the lowest severity of Rhizoctonia root rot relative to other amended soils. Phytotoxicity arising from the use of Brassicaceae SMs was observed particularly in soils amended with high glucosinolate-containing SMs. These studies demonstrate that Brassicaceae SMs can be used to manage disease caused by R. solani AG-8. However, future studies will need to focus on strategies for diminishing the crop growth-reducing effects associated with Brassicaceae SM amendment to fully maximize these fungicidal benefits.     

Using the Gluten Peak Tester as a tool to measure physical properties of gluten

The functional properties of wheat are largely dictated by composition and interactions of the gluten proteins. All flours contain gliadin and glutenin, but produce baked products of varying quality, which provides evidence that gluten proteins from different wheats possess different properties. A common method to study differences in gluten properties, which is utilized in this study, is fractionation/reconstitution experiments to understand how various gliadin to glutenin ratios and how fractions from different wheat sources affect gluten aggregation properties. Gliadin and glutenin from a vital wheat gluten were fractionated with 70% ethanol and reconstituted at various gliadin to glutenin ratios. Gliadin and glutenin from a Canadian eastern soft, eastern hard and western hard wheat (14% moisture) were fractionated and substituted between flours at the native gliadin to glutenin ratio. Gluten combinations were evaluated with a Gluten Peak Tester at constant temperature and mixing. Varying gliadin to glutenin ratio showed that 50:50 is optimal for fast gluten aggregation while amount of glutenin dictates strength. Substitution experiments showed that replacing good quality gluten fractions with those from a lower quality wheat decreases gluten quality, and vice versa. Data also showed that cultivar specific differences in gliadin and glutenin are more important in dictating gluten strength (torque), while gliadin to glutenin ratio dictates aggregation time (PMT) independent of the source of fractions. The study demonstrated the ability of the improved method to evaluate gluten aggregation by controlling for all variables except the one being tested. The data also revealed information about gluten aggregation properties never before seen.