Soil inoculation method determines the strength of plant–soil interactions

There is increasing evidence that interactions between plants and biotic components of the soil influence plant productivity and plant community composition. Many plant–soil feedback experiments start from inoculating relatively small amounts of natural soil to sterilized bulk soil. These soil inocula may include a variety of size classes of soil biota, each having a different role in the observed soil feedback effects. In order to examine what may be the effect of various size classes of soil biota we compared inoculation with natural field soil sieved through a 1 mm mesh, a soil suspension also sieved through a 1 mm mesh, and a microbial suspension sieved through a 20 μm mesh. We tested these effects for different populations of the same plant species and for different soil origins.Plant biomass was greatest in pots inoculated with the microbial suspension and smallest in pots inoculated with sieved soil, both in the first and second growth phase, and there was no significant population or soil origin effect. Plant-feeding nematodes were almost exclusively found in the sieved soil treatment. We show that processing the soil to obtain a microbial suspension reduces the strength of the soil effect in both the first and second growth phase. We also show that the results obtained with inoculating sieved soil and with a soil suspension are not comparable. In conclusion, when designing plant–soil feedback experiments, it is crucial to consider that soil inoculum preparation can strongly influence the observed soil effect.     

钙对盐胁迫下水稻幼苗抗氧化酶活性和膜脂过氧化作用的影响

研究了外源Ca2 对盐胁迫下耐盐性不同的两个水稻品种(武育粳3号和IR36)几种抗氧化酶活性及膜脂过氧化的影响。结果表明:适量的Ca2 供应能有效提高水稻叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)以及抗坏血酸过氧化物酶(APX)的活性,使之维持在较高的水平上,并降低了丙二醛(MDA)含量和细胞膜透性。此外,外源Ca2 还增加了抗坏血酸(AsA)的含量,增强了水稻幼苗的根系活力。这表明适量的外源Ca2 供应提高水稻耐盐性的原因之一,在于其增强了植株的活性氧清除能力以及对细胞膜的稳定作用。     

Community composition of ammonia-oxidizing bacteria in the rhizosphere of intercropped wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.), maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.), and faba bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.)

Cereal/cereal and cereal/legume intercropping systems are popular in the north, northwest, and southwest of China and often result in yield increases compared to monocropping. Rhizosphere interactions may play a significant role in the yield increases, particularly with respect to nutrient availability. The aim of this study was to investigate the effects of intercropping on N availability and community composition of ammonia-oxidizing bacteria in the rhizosphere of wheat, maize, and faba bean at different growth stages. Denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA genes was used to analyze the community composition of bacterial ammonia oxidizers belonging to β-proteobacteria. The results showed that intercropping with faba bean significantly increased nitrate concentrations in the rhizosphere of wheat and maize at the second sampling time (20 June) compared to monocropping or intercropping between maize and wheat. Intercropping significantly affected the community composition of ammonia-oxidizing bacteria in the rhizosphere compared to monocropping, and the effects were most pronounced in the maize/faba bean and wheat/maize intercropping systems when faba bean and wheat were at anthesis and maize was in seedling stage. In wheat/faba bean intercropping, the effects of intercropping on community composition of ammonia-oxidizing bacteria were less pronounced at the seedling stage of the two species but were significant at anthesis.     

长期施肥对红壤双季稻冬闲田春季杂草群落的影响

利用始于1982年的红壤双季稻田长期施肥定位试验,于2012年采用田间调查法研究了在无机肥(化肥NPK)与有机肥(M)氮磷钾养分等量条件下,长期施用有机肥、无机化肥和有机肥无机肥配施模式下红壤双季稻冬闲田春季杂草生物量及群落密度和群落多样性的变化。结果表明:有机肥无机肥配施改变了优势杂草种类,增加了杂草总密度和总生物量。化肥氮钾配施有机肥(NKM)处理的杂草种类数量最多,化肥氮磷配施有机肥(NPM)处理的最少。施肥处理中,M处理的杂草群落多样性指数、均匀度指数和优势度指数均最高,分别为1.118 6、0.732 3和0.629 7;其杂草总密度最低,为297.0株?0.25m?2。NPK处理的杂草总生物量最低,为58.0 g?0.25m?2。NPM处理的杂草群落多样性指数值、均匀度及优势度指数值均显著低于其他处理;其杂草总密度和杂草总生物量均最高,分别为539.7株?0.25m?2和109.5 g?0.25m?2;其优势杂草日本看麦娘的密度为428.0株?0.25m?2,相对密度为79.31%,明显高于其他处理优势杂草的密度和相对密度。春季杂草总干物质量与土壤碱解氮正相关(相关系数为0.703),与土壤p H负相关(相关系数为0.697),相关性不显著;与土壤有效磷显著正相关(相关系数为0.758*)。长期不同施肥模式下红壤稻田磷素是导致田间春季杂草群落特征变化的主要因素,通过改变土壤有效磷和碱解氮含量及土壤p H,能有效调控红壤双季稻冬闲田春季杂草生物量及群落密度和生物多样性。     

农用化学品及废弃物对土壤环境与食物安全的影响

综述了农用化学品和废弃物(化肥、农药、酸沉降、污水污泥、畜禽粪便和“三废”等)利用现状及其对土壤环境污染与食物安全的影响。     

土壤酸度对大豆、油菜生长和产量的影响

 两个盆栽试验结果显示，施用石灰石粉和钙镁磷肥能显著促进大豆生长和根瘤形成，并能提高产量，施用石灰增加了大豆植株Ca含量，但减少其K含量；施用磷肥增加植株的P含量，但减少植株的Ca的含量，大豆的根瘤数和籽粒产量与土壤pH、交换性Ca和Al及铝饱和度之间呈显著相关。10年的石灰石粉长期定位试验结果表明，在施用钙镁磷肥的条件下，施用3750kg/hm#+2石灰石粉即可使油菜获得高产，但适当过量施用石灰并未使油菜减产。要使红壤油菜获得最佳产量，必须使土壤pH值提高到5.9以上,而交换性铝降至1.0cmol(+)/kg以下。     

Burrow systems made by Aporrectodea nocturna and Allolobophora chlorotica in artificial cores: morphological differences and effects of interspecific interactions

Burrow systems of earthworms contained in artificial cores were analysed through X-ray computed tomography and 3D skeleton reconstructions. Gas diffusion experiments were carried out on these cores to characterize soil transfer properties associated with the different burrow systems. Three types of cores were studied: cores in which Aporrectodea nocturna, an anecic earthworm was introduced (treatment 1), cores in which Allolobophora chlorotica, an endogeic earthworm was introduced (treatment 2) and cores that contained both species (treatment 3). Comparisons of the characteristics of the burrow systems of treatment 1 and 2 show important differences: the burrow system of A. nocturna comprises fewer burrows, which are longer, less branched, more vertical and have a lower sinuosity. The burrow system of A. chlorotica is characterized by lower continuity, which however did not result in a lower soil diffusivity. To study the burrow systems made by the two species in the same core (treatment 3), a separation that was based on differences in pore diameter between the two species and that takes into account the burrow orientation was designed. This separation was proven to be efficient since it resulted in low percentages of errors (around 10%) when applied to the burrow systems of treatments 1 and 2. Comparison of the burrow systems from treatments 1 and 3 demonstrated that the burrow system of A. nocturna was influenced by the presence of A. chlorotica: in treatment 3, A. nocturna made burrows that were smaller, more vertical and less branched. However, these interactions have to be confirmed under natural conditions.     

Distribution of transformed organic matter in structural units of loamy sandy soddy-podzolic soil

The effect of land use types and fertilizing systems on the structural and aggregate composition of loamy sandy soddy-podzolic soil and the quantitative parameters of soil organic matter has been studied. The contribution of soil aggregates 2–1 mm in size to the total C_org reserve in the humus horizon is higher than the contributions of other aggregates by 1.3–4.2 times. Reliable correlations have been revealed between the contents of total (C_org), labile (C_lab), and active (C₀) organic matter in the soil. The proportion of C₀ is 44–70% of C_lab extractable by neutral sodium pyrophosphate solution. The contributions of each of the 2–1, 0.5–0.25, and <0.25 mm fractions to the total C₀ reserve are 14–21%; the contributions of each of the other fractions are 4–12%. The chemically labile and biologically active components of humic substances reflect the quality changes of soil organic matter under agrogenic impacts. A conceptual scheme has been proposed for the subdivision of soil organic matter into the active, slow (intermediate), and passive pools. In the humus horizon of loamy sandy soddy-podzolic soil, the active, slow, and passive pools contain 6–11, 34–65, and 26–94% of the total C_org, respectively.     

肥料运筹对砂姜黑土甘薯生长和产量的影响

氮肥氮肥易引起甘薯徒长而使产量下降,生产上农民一般采用较低施氮量确保甘薯稳产,但也影响了甘薯产量潜力的发挥。笔者通过田间试验研究了施肥量和钾肥品种运筹对砂姜黑土甘薯生长和产量的影响,为甘薯高产创建提供施肥依据。试验表明：施肥促进甘薯地上部生长大于促进块根的生长,且施肥量越大,根冠比下降越多。全部用硫酸钾高量施肥1导致甘薯地上部徒长,与习惯施肥相比,甘薯减产3.7%;钾肥一半用氯化钾的高量施肥2处理能使甘薯地上部和地下部协调生长,甘薯比习惯施肥增产6.2%,比高量施肥1增产10.4%,达显著差异水平。将习惯施肥氮肥用量124.5 kg/hm2提高到225 kg/hm2时,氯化钾是“忌氯”作物甘薯产量进一步提高关键,与单施硫酸钾相比,氯化钾与硫酸钾配施显著提高了甘薯N、P、K养分利用效率。试验表明砂姜黑土区甘薯不施含氯钾肥的习惯施肥不利于甘薯的高产,甘薯钾肥品种运筹值得进一步深入研究。