Characterization of Nitrate-Reducing and Amino Acid–Using Bacteria Prominent in Nitrotoxin-Enriched Equine Cecal Populations

Horses can be at risk for nitropoisoning by consuming plants containing 3-nitro-1-propionic acid or 3-nitro-1-propanol and to a lesser extent by plants containing nitrate. Populations of equine cecal microbes enriched for enhanced rates of 3-nitro-1-propionic acid (NPA) or nitrate metabolism were cultured for NPA- or nitrate-metabolizing bacteria on basal enrichment medium or tryptose soy agar supplemented with either 5-mM NPA or nitrate and under H₂:CO₂ (20:80) as the energy source. After 72 hours, separated colonies picked from plates, or roll tubes were cultured in fresh broth medium for 72 hours and then identified by 16S rRNA gene sequencing. Isolates from the NPA-enriched populations were identified as Streptococcus lutetiensis (five strains), Escherichia coli (two strains), and Sporanaerobacter acetigens (one strain). Strains isolated from nitrate-enriched populations were identified as Escherichia coli (one strain) and Wolinella succinogenes (three strains). None of these strains degraded NPA. Enriched populations of equine cecal microbes, the isolated pure strains and the type strain of Denitrobacterium detoxificans, a competent NPA-metabolizing microbe, were examined using denaturing gradient gel electrophoresis (DGGE). The DGGE analysis indicated that none of the strains in the enriched population of equine cecal microbes were similar to D. detoxificans. However, we report for the first time the isolation of the anaerobic amino acid–using Sporanaerobacter acetigenes from the equine cecum.     

种植密度对滴灌马铃薯生长、产量的影响

为了了解耕培土滴灌条件下种植密度对马铃薯生长、产量以及水分利用效率的影响.试验共设密度分别为7.28×10⁴ 株/hm²(RS25),6.67×10⁴ 株/hm²(RS35),5.55×10⁴ 株/hm²(CK)3个处理.结果表明:随着种植密度的增加,株高、茎粗、干物质积累量以及商品薯率均有降低的趋势;产量、水分利用效率随种植密度的增加表现出先增大后减少,其中RS35处理产量和水分利用效率均表现最高,分别达到47 325 kg/hm²和12.05 kg/m³;在马铃薯品质方面,种植密度对马铃薯粗蛋白含量的影响不具有统计学意义;淀粉和维生素C随着密度的大幅增加而降低,其中RS25处理的淀粉较CK降低了5.57%,RS25处理的维生素C较CK降低了7.96%,同时RS35与CK处理不具有统计学意义.综上所述,种植密度为6.67×10⁴ 株/hm²的RS35处理马铃薯高产优质,且水分利用效率最高,为黑龙江地区滴灌马铃薯较为适宜的种植密度.     

砂田抑制蒸发功能随覆砂年限的演变规律

【目的】研究压砂覆盖(砂田)保温及抑制蒸发功能随砂田退化程度的演变规律。【方法】基于多年Landsat卫星数据,使用辐射传输方程法反演香山地区砂田地表温度(LST),结合田间地表温度监测,对比砂田与裸地的地表温度变化,分析了砂田抑制土壤蒸发的机理,并探讨了砂田的功效与砂田使用年限的关系。【结果】砂田在LST-NDVI梯形空间中贴近于暖边,其土壤水分比耕地少,接近于干土层。因此,砂田可以隔离辐射与土壤表层,从而减小潜热通量,抑制土壤蒸发。砂田昼夜温差明显比裸地大,且对于西瓜等作物,砂田的有效地表积温也比裸地提高了10%。【结论】砂田退化过程可分为纯砾石阶段、砂土混合阶段和砂土连通阶段,从砂田的保温及抑制蒸发功能来看,砂田的有效使用年限为25～30 a。     

How do timing,duration, and intensity of drought stress affect the agronomic performance of winter rye?

Winter rye (Secale cereale L.) will be especially affected by drought induced yield losses in Central and Eastern Europe in the future because it is predominantly cultivated on low-fertile soils with a poor water-holding capacity. In order to examine the performance of winter rye under different drought conditions, field experiments were carried out during the years 2011, 2012, and 2013 near Braunschweig, Germany. Two sets of genotypes were tested under severe, mild, pre-anthesis, and post-anthesis drought stress in rain-out shelters as well as under rainfed and well-watered conditions. The grain, straw, and total above ground biomass yields, harvest index, grain yield components, leaf area index (LAI), and phenological characteristics were examined, as well as phenotypic correlations between grain yield and further characteristics. Drought induced grain yield reduction ranged from 14 to 57%, while straw yield and harvest index were lesser affected by drought than the grain yield. Under drought conditions, fully ripe was reached up to twelve days earlier than under non water-limited conditions. Pre-anthesis drought mainly reduced spikes m⁻² and kernels spike⁻¹ while drought during grain filling reduced the 1000-kernel weight (TKW) only. The grain yield was positively associated with straw yield, spikes m⁻², and kernels spike⁻¹ under water limited conditions while the TWK was only positively associated with grain yield under drought during grain filling. Consequently, high pre-anthesis biomass as well as high numbers of spikes m⁻² and kernels spike⁻¹ are especially important for obtaining high grain yields under water-limited conditions. Focusing on these traits is, therefore, recommendable for developing drought tolerant rye genotypes.     

盐阳离子类型对红壤裂缝开闭规律的影响

为了探究灌溉水中盐离子浓度及类型对土壤裂缝变化过程的影响,为南方灌溉用水安全提供数据理论支持,采用蒸馏水配置15,10,5和2.5 g/L等4个质量浓度梯度的NaCl,KCl,CaCl₂和MgCl₂等4种类型溶液,分别浸泡南方亚热带地区的典型红壤,并且采用恒温蒸发模拟干燥过程和喷湿法模拟增湿过程,结合数字图像技术提取土壤表层开裂闭合过程中裂缝主要几何参数,分析盐阳离子类型对红壤裂缝发育过程特征的影响.结果表明:红壤干燥过程中,裂缝在土壤质量含水率由30%降至10%时增长最快;在质量含水率低于10%后达到最大值并保持稳定不变.红壤的裂缝面积密度与4种盐阳离子浓度呈极显著正相关关系,裂缝长度密度与K⁺及Mg²⁺呈极显著负相关,与Ca²⁺呈显著负相关关系.4种盐阳离子对红壤开裂面积促进程度深浅顺序为Na⁺,K⁺,Ca²⁺,Mg²⁺;4种不同离子浓度的处理中,NaCl质量浓度为10 g/L,而KCl,CaCl₂和MgCl₂质量浓度为15 g/L的溶液对红壤开裂促进效果最好,其裂缝面积相比于蒸馏水处理分别增大了155.0%,160.1%,109.4%和39.2%.红壤的开裂含水率低于闭合含水率,4种盐阳离子浸泡后会使红壤的开裂含水率和闭合含水率均升高.     

基于机器视觉的育肥猪分群系统设计与试验

为控制育肥猪出栏时的体质量差异,该研究开发了一套基于机器视觉技术的育肥猪分群系统,该系统通过机器视觉技术和卷积神经网络模型代替传统地磅对猪只体质量进行估测,可有效避免粪污对设备精度的影响及腐蚀;以前一天全部猪只体质量数据从小到大排列的第30%个数据作为当日的分群基准质量,将大于等于基准质量的视为长势较快的猪只,小于基准质量的视为长势较慢的猪只,每次采食按照猪只长势快慢分为2群进行饲喂;该系统依托于LabVIEW软件开发平台和物联网系统构建,平均每头猪只通过系统时间为6.2 s。为验证该系统的实际应用效果开展了为期30 d的现场试验,将饲喂于装有分群系统猪栏中的120头长白育肥猪作为试验组,由分群系统按猪只长势快慢分群饲喂;将饲喂于传统猪栏中的120头长白育肥猪作为对照组,按照传统人工调栏的方式进行饲喂。试验开始时试验组和对照组猪只平均体质量分别为32.21、31.76 kg,标准差分为别2.61和2.49 kg;结束时试验组和对照组猪只平均体质量分别为57.68、57.41 kg,标准差分为别5.26和5.51 kg,总料肉比分别为2.31和2.34,期间试验组猪只体质量的标准差小于对照组,但是2组猪只平均体质量、标准差、总料肉比均不存在显著差异,表明采用该系统对猪只进行分群饲喂控制猪只体质量差异效果等同于人工调栏,同时可以节省人力成本,缓解农业劳动力短缺的压力。该研究也可为母猪饲喂站、种猪测定站等智能化养猪设备的研发提供参考。     

基于氧同位素的玉米农田蒸散发估算和区分

农田蒸散发(evapotranspiration,ET)的估算和区分是土壤-植物-大气连续体中的重要研究内容,是农业水资源高效利用的重要基础。该研究分析了土壤水、蒸发水汽、蒸腾水汽和大气背景混合水汽氧同位素组成分布特征,并采用2种同位素的方法对玉米农田蒸散发进行估算和区分:1)结合Keeling plot和Craig-Gordon模型的同位素方法(Iso-CG);2)基于土壤水同位素守恒和水量平衡的方法(Iso-WB)。结果表明,在玉米生育期内Iso-WB方法与Iso-CG方法所计算的玉米蒸腾比例分别为0.64~0.91和0.52~0.91,平均值分别为0.80和0.78。玉米蒸散发总量在前期、中期和后期均值分别为3.95、5.30和4.98 mm/d。通过比较参数并与前人研究结果对比分析,表明采用Iso-CG方法估算区分ET相对精确,采用Iso-WB方法计算蒸散发要求的测量精度相对较高,计算误差较大。该研究成果不仅为玉米农田制定灌溉制度及提高用水效率提供了理论依据,而且对深入探索氧同位素水文学领域具有重要意义。     

规模化奶牛场经营管理研究

进入21世纪以来,中国奶业步入了快速发展期,规模化奶牛场经营管理的科学研究已经成为每个奶牛场经营管理者的必修课程和首要任务。本文对规模化奶牛场经营管理效能的相关影响因子进行了系统的综述,分析了规模化奶牛场经营管理的国内外研究现状、取得的成果以及存在的不足,并对未来我国规模化奶牛场经营管理的研究方向进行了展望。     

挠力河流域水基系统健康评价及指标体系研究

水生态环境遭到不同程度的破坏,流域健康状况越来越受到重视。水基系统的提出为流域健康评价提供了新的理论框架。现以挠力河流域为研究基点,根据其国家农产品主产区的特点,在水基系统的理论框架下,研究了适合该流域的健康评价等级标准,并依据指标选取5项原则、流域的自然经济状况构建了挠力河流域的水基系统健康评价指标体系。筛选了以农业生产和可持续发展为主导的评价指标集,分析了特定指标对该研究区域的适用性。所建立水基系统健康评价体系和指标集可为挠力流域的可持续发展提供技术支持。