Two new diterpene derivatives from Euphorbia lunulata Bge and their anti-proliferative activities

A new ent-abietane-type diterpene lactone (1) and a new jatrophane-type diterpenoid (2), together with twelve known compounds including three diterpenes (3–5), five triterpenes (6–10) and four sterides (11–14) were isolated from the ethanol extract of the whole plant of Euphorbia lunulata Bge. The structure of compounds 1 and 2 was elucidated on the basis of 1D and 2D NMR spectra and the HR-ESI-MS data. The structure of compound 2 was further analyzed by an X-ray crystallographic study. The in vitro anti-proliferative activities against MCF-7 and NCI-H460 cell lines for compounds 1–5 (diterpene) were evaluated. The results showed marked activity for compound 1 against the two cell lines with the IC₅₀ values 19.5 (NCI-H460) and 18.6 (MCF-7) μM, while for cis-platinum (a positive cytotoxic control agent) 29.7 (NCI-H460) and 27.7 (MCF-7) μM. Compounds 2–5 exhibited moderate cytotoxic activities for the two cell lines with the IC₅₀ values ranging from 32.1 to 58.2 μM.     

Land surface temperature retrieval for arid regions based on Landsat-8 TIRS data:a case study in Shihezi,Northwest China

Scientific interest in geophysical information about land surface temperature （LST） is ever increasing, as such information provides a base for a large number of applications, including environmental and agricultural monitoring. Therefore, the research of LST retrieval has become a hot topic. Recent availability of Landsat-8 satel- lite imagery provides a new data source for LST retrieval. Hence, exploring an adaptive method with reliable ac- curacy seems to be essential. In this study, basing on features of Landsat-8 TIRS thermal infrared channels, we re-calculated parameters in the atmospheric transmittance empirical models of the existing split-window algorithm, and estimated the ground emissivity with the help of the land cover classification map of the study area. Further- more, a split-window algorithm was rebuilt by virtual of the estimation model of the updated atmospheric transmit- tance and the ground emissivity, and then a remote sensing retrieval for the LST of Shihezi city in Xinjiang Uygur autonomous region of Northwest China was conducted on the basis of this modified algorithm. Finally, precision validation of the new model was implemented by using the MODIS LST products. The results showed that the LST retrieval from Landsat-8 TIRS data based on our algorithm has a higher credibility, and the retrieved LST is more consistent with the MODIS LST products. This indicated that the modified algorithm is suitable for retrieving LST with competitive accuracy. With higher resolutions, Landsat-8 TIRS data may provide more accurate observation for LST retrieval.     

1988-2018年哈密绿洲植被覆盖度时空变化及其驱动力

[目的] 对近30 a来哈密绿洲植被覆盖度时空变化及驱动力进行分析，为该地区绿洲持续健康发展提供理论借鉴。[方法] 以1988，1998，2008及2018年4期影像为基础数据，利用像元二分模型计算了基于AFRI_SWIR2指数提取的植被覆盖度，利用动态度指数与植被覆盖度转移矩阵分析哈密绿洲植被覆盖度动态变化；再通过地理探测器对8个影响因子进行探测，探寻哈密绿洲植被覆盖度的驱动因子。[结果] 近30 a来哈密绿洲面积从1988年的214 km²增长到2018年的632.1 km²，增幅达195%，但绿洲内植被覆盖度等级却较低，同时哈密绿洲面积主要扩展的区域在西北部和东南部区域；哈密绿洲的发展阶段可分为3个阶段：1988-1998年为低强度稳定期，1998-2008年为极度扩张期，2008-2018年为高强度稳定期。[结论] 土地利用类型变化是造成哈密绿洲植被覆盖度变化的直接原因，同时在近30 a内，人为因素对哈密绿洲植被覆盖状况的影响远高于自然因素。     

巴音布鲁克新疆假龙胆适生地植物群落物种多样性及其环境解释

为研究新疆巴音布鲁克地区不同土壤层次环境因子对新疆假龙胆适生地植物群落物种多样性的影响,为新疆假龙胆物种多样性保护提供理论依据。采用野外样地调查的方法,在新疆假龙胆适生海拔范围内共设置了16个样地进行植物多样性统计分析,共调查到植物72种,隶属22科48属,以豆科和禾本科植物为主。根据样地内各物种重要值的大小将所有采样点的植物群落划分为5类。通过单因素方差分析和RDA分析,对植物群落多样性指数与土壤环境因子间的关系进行了分析,结果表明:物种多样性指数与土壤环境因子之间具有显著的相关关系,其中土壤全磷、全氮、总盐是影响物种多样性的主导因素。对植物群落多样性指数与土壤不同层次理化指标的进一步分析表明:影响植物群落物种多样性极显著的环境因子是0~20cm土层的全磷,其次分别是0~10cm土层的全钾和30~50cm土层的总盐和pH值。     

奎屯城市绿化落叶彩叶植物应用调查与分析

对奎屯市园林常见落叶彩叶植物种类及应用的调查研究结果表明,目前奎屯市常见落叶彩叶植物共有28种,隶属17科。文内具体介绍了落叶彩叶植物在奎屯市园林绿化中的配置方式,并就解决落叶彩叶植物应用中存在的问题提出了建议。     

Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil

Ammonia oxidation is a critical step in the soil nitrogen (N) cycle and can be affected by the application of mineral fertilizers or organic manure. However, little is known about the rhizosphere effect on the function and structure of ammonia-oxidizing bacterial (AOB) and archaeal (AOA) communities, the most important organisms responsible for ammonia oxidation in agricultural ecosystems. Here, the potential nitrification activity (PNA), population size and composition of AOB and AOA communities in both the rhizosphere and bulk soil from a long-term (31-year) fertilizer field experiment conducted during two seasons (wheat and maize) were investigated using the shaken slurry method, quantitative real-time polymerase chain reaction and denaturing gradient gel electrophoresis. N fertilization greatly enhanced PNA and AOB abundance, while manure application increased AOA abundance. The community structure of AOB exhibited more obvious shifts than that of AOA after long-term fertilization, resulting in more abundant AOB phylotypes similar to Nitrosospira clusters 3 and 4 in the N-fertilized treatments. Moreover, PNA was closely correlated with the abundance and community structure of AOB rather than that of AOA among soils during both seasons, indicating that AOB play an active role in ammonia oxidation. Conversely, the PNA and population sizes of AOB and AOA were typically higher in the rhizosphere than the bulk soil, implying a significant rhizosphere effect on ammonia oxidation. Cluster and redundancy analyses further showed that this rhizosphere effect played a more important role in shaping AOA community structure than long-term fertilization. Overall, the results indicate that AOB rather than AOA functionally dominate ammonia oxidation in the calcareous fluvo-aquic soil, and that rhizosphere effect and fertilization regime play different roles in the activity and community structures of AOB and AOA.     

Development of a vision-based automatic vaccine injection system for flatfish

Traditionally, flatfish vaccination has been performed manually a laborious and time-consuming, procedure with low accuracy. The handling requirement also makes it prone to contamination. With a view to eliminating these drawbacks, we designed an automatic vaccine system in which the injection is delivered by a Cartesian coordinate robot (also called a linear robot) guided by a vision system. The automatic vaccine injection system is driven by an injection site location algorithm that uses a template-matching technique. The proposed algorithm was designed to derive the time and possible angles of injection by comparing a search area with a template. The algorithm is able to vaccinate various sizes of flatfish, even when they are loaded at different angles. We validated the performance of the proposed algorithm by analyzing the injection error according to randomly generated loading angles. The proposed algorithm allowed an injection rate of 2000 per hour on average. Vaccination of flatfish with a body length of up to 500 mm was possible, even when the orientation of the fish was random. The injection errors in various sizes of flatfish were very small, ranging from 0 to 0.6 mm.     

Combined treatment of aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) reduces melting-flesh peach fruit softening

The effects of postharvest application of aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) on ethylene production and fruit quality, and thus on transportation and shelf-life, were evaluated in melting-flesh peaches. AVG (150 mg L⁻¹) significantly reduced ethylene production, and the effect was enhanced in combination with 1-MCP (1 μL L⁻¹). However, fruit treated with AVG alone softened to untreated control levels 2 d after harvest (DAH). Treatment with 1-MCP significantly reduced the rate of softening until 2 DAH, but the fruit rapidly softened thereafter, and reached untreated control levels by 4 DAH. A combination of AVG and 1-MCP significantly reduced fruit tissue softening throughout ripening. The effect of each chemical on flesh firmness indicated that 1-MCP affected fruit response in the early stages of ripening up to 4 DAH, and AVG significantly reduced softening in the latter stages from 4 to 9 DAH. Peaches treated with AVG and 1-MCP retained their ground color during ripening, but the effect of each chemical on color is unclear. The present study indicates that combined treatment with AVG and 1-MCP significantly delays the ripening of melting-flesh peaches.     

Implication of limonene and linalyl acetate in cytotoxicity induced by bergamot essential oil in human neuroblastoma cells

Bergamot (Citrus bergamia, Risso et Poiteau) essential oil (BEO) is a widely used plant extract showing anxiolytic, analgesic and neuroprotective effects in rodents; also, BEO activates multiple death pathways in cancer cells. Despite detailed knowledge of its chemical composition, the constituent/s responsible for these pharmacological activities remain largely unknown. Aim of the present study was to identify the components of BEO implicated in cell death. To this end, limonene, linalyl acetate, linalool, γ-terpinene, β-pinene and bergapten were individually tested in human SH-SY5Y neuroblastoma cultures at concentrations comparable with those found in cytotoxic dilutions of BEO. None of the tested compounds elicited cell death. However, significant cytotoxicity was observed when cells were cotreated with limonene and linalyl acetate whereas no other associations were effective. Only cotreatment, but not the single exposure to limonene and linalyl acetate, replicated distinctive morphological and biochemical changes induced by BEO, including caspase-3 activation, PARP cleavage, DNA fragmentation, cell shrinkage, cytoskeletal alterations, together with necrotic and apoptotic cell death. Collectively, our findings suggest a major role for a combined action of these monoterpenes in cancer cell death induced by BEO.     

玫瑰花红色素及其稳定性研究

对玫瑰花色素作为新型天然食用色素的可行性进行了初步研究。通过纸色谱法、紫外分光光度法、酸碱反应、氯化亚铁显色反应、盐酸镁粉反应、中性醋酸铅沉淀反应等。初步鉴定了化学成份。对光、温度、ＰＨ值、氧化介质、还原价质、蔗糖、食盐、铝制品、铁制品、铜制品、锡制品等对比色素的影响，进行了初步探讨，结果表明，其主要成份为黄酮类化合物；对光、热耐受性好；适用ＰＨ值范围宽；耐氧化性较差，耐还原性较好；Ｆｅ＾３＋和Ｓ