黄淮海地区不同种植结构下点蜂缘蝽的发生规律

为明确大豆-玉米带状间作种植模式对点蜂缘蝽Riptortus pedestris发生规律的影响,2023年在山东省大豆单作田中进行诱捕效果试验,筛选诱捕效果最好的诱捕器对黄淮海地区山东、河南和安徽3个省6个市不同种植模式下点蜂缘蝽的发生动态进行监测。结果显示,风叶型诱捕器对点蜂缘蝽的诱捕效果最佳。在大豆单作田内点蜂缘蝽的发生高峰期为7月中旬—8月中旬,此时大豆正值盛花-始荚期。在大豆-玉米带状间作田内点蜂缘蝽的发生动态与大豆单作田的无明显差异,但点蜂缘蝽发生数量及高峰期发生时间可能与杀虫剂的施用时间和次数密切相关。     

Identification,Characteristics and Function of Phosphoglucomutase (PGM) in the Agar Biosynthesis and Carbon Flux in the Agarophyte Gracilariopsis lemaneiformis (Rhodophyta)

Agar is widely applied across the food, pharmaceutical and biotechnology industries, owing to its various bioactive functions. To better understand the agar biosynthesis in commercial seaweed Gracilariopsis lemaneiformis, the activities of four enzymes participating in the agar biosynthesis were detected, and phosphoglucomutase (PGM) was confirmed as highly correlated with agar accumulation. Three genes of PGM (GlPGM1, GlPGM2 and GlPGM3) were identified from the G. lemaneiformis genome. The subcellular localization analysis validated that GlPGM1 was located in the chloroplast and GlPGM3 was not significantly distributed in the organelles. Both the GlPGM1 and GlPGM3 protein levels showed a remarkable consistency with the agar variations, and GlPGM3 may participate in the carbon flux between (iso)floridoside, floridean starch and agar synthesis. After treatment with the PGM inhibitor, the agar and floridean starch contents and the activities of floridean starch synthase were significantly decreased; products identified in the Calvin cycle, the pentose phosphate pathway, the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cycle were depressed; however, lipids, phenolic acids and the intermediate metabolites, fructose-1,6-phosphate were upregulated. These findings reveal the essential role of PGM in regulating the carbon flux between agar and other carbohydrates in G. lemaneiformis, providing a guide for the artificial regulation of agar accumulation.     

坛紫菜自由丝状体对无机碳的利用

Free-living Porphyra haitanensis conchocelis was cultured under continual light,then it was treated with Van,DIDS and SITS,the inhibitors of inorganic carbon utilization,to study the mechanism of inorganic carbon utilization in the conchocelis.The results indicate that Van inhibits the utilization of inorganic carbon most,with a rate of 71.3%,and the rates of DIDS and SITS are relatively lower,42% and 35.5% respectively.The inhibition rate of Az is 25.3%,which indicates the external CA is not an important part of its inorganic carbon uptake.A complementary conclusion is that the main part of inorganic carbon absorption in P.haitanensis conchocelis is through active transport of HCO3^- and CO2.     

浙江沿海三疣梭子蟹的养殖模式

为开发三疣梭子蟹低盐耐受性相关甲基化标记,采用甲基化敏感性高分辨率熔解曲线法(methylation sensitive high resolution melting curve, MS-HRM)进行了三疣梭子蟹低盐耐受性相关甲基化位点的筛选和应用。结果显示,从三疣梭子蟹转录组数据库中共开发了8个甲基化标记,其中6个标记与低盐度耐受性显著相关,在低盐度条件下表现出显著的去甲基化或甲基化,这些标记分别位于V-ATP酶(V-type proton ATPase)、CLC型氯离子通道、琥珀酸脱氢酶、NAD(P)转氢酶(NAD(P)、磷酸乙醇酸性磷酸酶、NADH还原酶基因,为三疣梭子蟹耐低盐新品种的分子标记辅助选育提供候选工具。研究表明,“宁象1号”耐低盐能力显著高于野生群体,且选育群体中位点Pt-M1、Pt-M2的去甲基化率达到100%,可能有利于低盐条件下保持体内Na⁺和Cl⁻的平衡。     

饲料中维生素A对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢酶活性及基因表达的影响

为研究饲料中维生素A(VA)对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢相关酶活性及基因表达的影响,实验选取360尾初始体质量为(6.10±0.10) g的青鱼幼鱼,随机分配至3个实验组中,每个实验组设置3个平行。采用单因素实验设计,以无维酪蛋白和明胶为蛋白源、菜籽油为脂肪源、糊精为糖源,同时添加矿物质混合物和维生素混合物(无VA添加)配制成3组实验饲料,分别以饲料1 (Diet1)、饲料2 (Diet2)和饲料3 (Diet3)表示。在饲料1、饲料2和饲料3中分别添加0、2 200和20 000 IU/kg VA醋酸酯(500 000IU/g),经高效液相色谱法(Agilent-1100, Agilent,美国)检测后实验饲料中VA的实际含量分别为178.2、2 058.9和18 436.2 IU/kg,养殖周期为8周。结果显示:饲料中VA缺乏会显著降低青鱼幼鱼的增重率(WGR)和特定生长率(SGR);VA缺乏会显著降低血清血糖(GLU)、甘油三酯(TG)和低密度脂蛋白(LDL)浓度,增加总胆固醇(TCH)浓度。饲料中添加2 058.9IU/kg VA能显著提高肝脏己糖激酶(HK)、磷...     

Comparison of the influences of vegetation stem parameters on hydraulic variables and sediment transport capacity

The hydraulic variables (Hv) and sediment transport capacity (Tc) of overland flow have changed immensely due to large-scale revegetation. However, research comparing the influences of stem parameters (diameter, cover, and arrangement) on Hv and Tc is limited. The objectives of this study were to explore and compare the influences of stem parameters on Hv and Tc. Data on three treatment groups with varying stem diameters, covers, arrangements, flow discharges and slopes were collected in this study. With increasing stem diameter, Hv and Tc increased; Hv included the Reynolds and Froude numbers, flow velocity (v), shear stress (τ), stream power (Ω), and unit stream power (ω). However, the trend of the Darcy-Weisbach friction coefficient (f) was opposite. Hv and Tc were significantly influenced by stem diameter and cover. The effect of stem diameter on Hv and Tc was greater than the effects of cover and arrangement. Stem cover as a variable could not be used to adequately estimate the Tc when there were various stem diameters. A new exponential equation involving stem cover and stem diameter was demonstrated to be an appropriate predictor of Tc. Stem diameter and arrangement had no obvious influence on the relationship between Tc and v, and v as an indicator could explain the effect of stem parameters on Hv and Tc. This result could illustrate why the variables, including v, were able to predict Tc under vegetation stem and litter cover.     

竹资源在发展生态旅游中的文化价值

生态旅游作为旅游开发的一大热点,在开发中面临着如何丰富、深化其内涵的问题.我国是一个竹资源丰富的国家,该文就竹资源在发展生态旅游中的价值从竹子的观赏造景功能、文化承载功能、旅游商品开发价值等方面进行了探讨.     

钢-竹组合楼板受弯试验及变形分析

为了探讨界面滑移效应对钢-竹组合楼板变形的影响,进行6块组合楼板受弯性能试验.试件共分为2组,一组为冷弯薄壁型钢与竹胶板界面仅周结构胶复合,另一组则在胶粘基础上用自攻螺钉强化,对组合楼板破坏形态、承载能力、跨中变形、界面相对滑移等进行研究.分析表明：第一组试件考虑滑移效应的跨中变形计算值与实测值吻合较好,因考虑了跨中附加挠度的影响,理论计算结果与换算截面法相比,其精度大约提高5％,更符合实际情况;第二组由于自攻螺钉的强化作用,整体性能提高很多,材料进入塑形阶段,所建理论模型不符合试验情况,有待进一步研究.     

四明山国家森林公园游客游憩动机

【目的】研究四明山国家森林公园游客游憩动机类型及差异,为四明山国家森林公园旅游产品升级转型提供科学依据。【方法】采用问卷调查法对四明山国家森林公园游客游憩动机进行实地调研,利用SPSS22.0软件对问卷调查结果开展描述统计分析、探索性因子分析、均值计算、独立样本t检验和单因素方差分析,使用AMOS21.0软件进行验证性因子分析。【结果】四明山国家森林公园游客的5个游憩动机均值表现为健康放松4.37>社会交往4.03>学习教育3.96>自我实现3.86>逃避压力3.79;不同学历游客在逃避压力动机上存在显著差异(P<0.05),不同职业游客在学习教育、自我实现以及逃避压力动机上均存在显著差异(P<0.05),不同家庭年收入的游客在学习教育、社会交往及健康放松动机上存在显著差异(P<0.05)。【结论】健康放松是四明山国家森林公园游客体验森林游憩活动的首要驱动因素,其次为社会交往、学习教育和自我实现,而逃避压力位居最后。在维护森林生态安全前提下,建议四明山国家森林公园开发森林康养、森林研学、森林度假、森林养老和森林体育等旅游产品。