徐州半翅目异翅亚目昆虫种类及危害调查

采用踏查与标准地调查相结合的方法,系统调查了徐州半翅目异翅亚目昆虫的种类、发生与危害情况。结果表明,徐州异翅亚目昆虫共有10科34种,较历史记载的种类(5科20种)有显著增加,其中,蝽科种类最多(13种),占调查总种数的38.2%,较历史记载新增5种;缘蝽科次之(7种),占20.6%,新增5种;网蝽科和猎蝽科分别新增1种和5种。重要林木害虫有麻皮蝽、茶翅蝽、稻棘缘蝽、点蜂缘蝽、悬铃木方翅网蝽、梨冠网蝽和小板网蝽等,其中3种网蝽均为中度以上发生,特别是悬铃木方翅网蝽为重度发生,其风险性R值为1.64。蝽科的益蝽、蠋蝽、蓝蝽和猎蝽科的黑红赤猎蝽、短斑普猎蝽、暴猎蝽、黑叉盾猎蝽和黄纹盗猎蝽均为捕食性天敌昆虫。     

促生内生真菌对环草石斛幼苗生理代谢的影响

研究了促生内生真菌S12、S50对环草石斛幼苗生物量、光合作用特征、酶活力、蛋白质和脯氨酸含量的影响.结果表明:菌株S12和S50对环草石斛的光合速率、蛋白质含量和生物量有显著提高,并在短时间内迅速提高环草石斛的叶绿素含量、Chla/Chlb值、脯氨酸含量以及SOD、POD和PAL活力.但随着时间的延长,其作用效果逐渐减弱并回到对照水平.     

牛催乳素受体基因遗传多态性及其与部分精液品质性状的关联分析

采用PCR-SSCP方法对种公牛群体的催乳素受体基因(PRLR)所有外显子及5′UTR多态性进行检测。结果表明:外显子1及外显子8分别检测到了2种等位基因A、B和C、D,其中A、C为群体中优势基因,其他外显子及5′UTR没有遗传多态性;相关性分析表明,第1外显子位点AA型鲜精顶体完整率显著高于AB型(P=0.012),第8外显子位点CD型冻精活力显著高于CC型和DD型(P=0.004)。初步推断PRLR为影响种公牛繁殖性状的一个候选基因。     

鸡胚成纤维细胞原代培养及应用

原代培养是指在体外模拟体内生理环境,使从体内取出的组织细胞生存、生长和传代,并维持原有组织细胞的结构和功能特性。鸡胚成纤维细胞具有相对容易获得、增殖能力强、适应性强、良好的耐受性、性状比较稳定等特点,使得其被广泛地应用于分子和细胞生物学研究的许多方面。论文综述了近年来有关鸡胚成纤维细胞的培养技术,如鸡胚的取材、细胞的分离、纯化和培养,同时介绍了应用进展和未来研究方向。     

核桃品种对黑斑病抗性的研究

作者用标准差法研究了20个核桃品种(系)对黑斑病的抗性,结果表明,供试品种(系)均可感病,但品种(系)间的抗性存在显著差异,其中‘陇32’和‘西林2’等2个品种(系)为高抗品种,‘陇11’‘陇15’‘维纳’‘扎343’‘辽3’等5个品种(系)为中抗品种,‘中林1’、A02、‘元丰’‘丰辉’‘西洛3’等5个品种为抗病品种,‘中林5’‘阿7’‘鲁光’‘辽1’‘辽4’‘西扶2’等6个品种为感病品种,‘香玲’和‘中林3’等2个品种为高感品种。     

Effects of up-regulation of c-myc expression on U937 cell line

AIM:To establish a human monocytic　leukemia cell line U937 stably expressing c-myc gene and to investigate the biological characteristics of this cell line. METHODS:The recombinant plasmid MSCV-c-myc-IRES-GFP (MMIG) was constructed. MMIG and MSCV-IRES-GFP (MIG) were used to package the viruses for infecting U937 cells. Fluorescence-activated cell sorter (FACS) was used for sorting U937/GFP and U937/MYC cells. The GFP-positive cells were detected by fluorescence microscopy and FACS. The protein expression of c-Myc, survivin, X-linked inhibitor of apoptosis protein (XIAP) and Bcl-2 was detected by Western blotting. Cell proliferation was evaluated by MTT assay. Propidium iodide (PI) staining was used to determine the cell cycle distribution. Self-renewal ability was observed by colony- forming assay. RESULTS:The GFP expression in the cells infected with MIG or MMIG virus was observed under fluorescence microscope. The green fluorescent rate of the cells infected with MIG was 26.0%, while that of the cells infected with MMIG was 27.7%. The protein expression of c-Myc in MMIG-infected U937 cells was higher than that in MIG-infected cells. After sorting, the green fluorescent rates of U937/GFP and U937/MYC cells reached 98.7% and 93.7%, respectively. The protein expression of c-Myc in U937/MYC cells was higher than that in U937/GFP cells. In addition, survivin, a downstream protein of c-Myc, was up-regulated, while the protein expression of XIAP and Bcl-2 remained unchanged. Cell cycle analysis showed that the percentage of the cells in S phase increased in U937/MYC cells. Moreover, the proliferation and colony-forming ability of U937/MYC cells were also enhanced. CONCLUSION: U937/MYC cell line stably expressing c-myc gene was successfully established. c-Myc may increase cell viability via enhancing the expression of anti-apoptotic protein survivin, the cell cycle transition and the self-renewal ability.     

日粮中添加不同植物油对奶牛瘤胃内环境的影响

以3头年龄相同、平均体重为(650±20)kg、泌乳期相同的装有永久性瘤胃瘘管的荷斯坦奶牛为试验动物,采用3×3拉丁方设计,研究探讨添加葵花油、胡麻油和菜籽油的日粮对奶牛瘤胃内纤毛虫数量、pH值、NH3-N浓度动态变化的影响。研究结果表明,日粮中添加不同的植物油(日粮脂肪含量均为6.5%)对奶牛瘤胃内纤毛虫数量、pH值、NH3-N浓度动态变化的影响差异均不显著(p>0.05)。     

6个紫肉甘薯新品系比较试验初报

以金山57和Ayamurasiky为对照,通过比较试验,初步分析了福建省泉州农业科学研究所新近选育的6个紫肉甘薯品种(系)的鲜薯产量、薯块烘干率、食味品质和花青素含量等主要经济性状,并评述了其在生产或育种上的利用价值。这些品系的育成,丰富了紫肉甘薯育种材料;同时提出以紫肉甘薯品种或中间材料为亲本,进行计划集团杂交或放任授粉,扩大基因背景和杂种后代的选择群体,是选育紫肉甘薯品种的有效途径之一。     

"泉薯系列"甘薯新品种选育研究

阐述了泉州市农业科学研究所"九五"以来甘薯新品种选育研究概况及进展.并介绍了泉薯647、泉薯23、泉薯11、泉薯52及叶菜型品种泉薯830的特征特性.     

苎麻开花结籽对其生长发育及产量、品质影响的研究

本研究通过大田试验和统计分析相结合的方法，探讨了苎麻开花结籽对其生长发育及产量和品质的影响，旨在为苎麻控花剂的研究和使用的必要性提供依据。结果表明：雌雄都不去和去雄、去雌、雌雄均去等四个处理在株高、茎粗、皮厚、鲜皮重、原麻产量、鲜皮出麻率、纤维支数等方面均不存在显著性差异，证明开花结籽对苎麻生长和发育、产量及品质没有显著影响。