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关于曲线在一点的基本三棱形的探讨 总被引:1,自引:0,他引:1
以弧长为参数,定义了曲线在一点的弗雷内标架与基本三棱形,推导出其对应量的一般参数方程. 相似文献
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给出了用组合数学中的递推关系H_n=■α_iH_(n-i)(其中α_1,α_2,…,α_r为常数)计算具有二阶及二阶以上常系数递推关系的n阶行列式的计算方法.通过实例给出此方法的解题步骤,并验证了其可行性. 相似文献
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ZHANG Shengying WU Xiaochun XING Xiaoyong LIU Jia YUE Yahui ZHANG Yangyang HE Jian WEN Fengqin BAO Shijun 《中国畜牧兽医》2007,47(10):3149-3157
The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis. 相似文献
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文章介绍了黑加仑嫩枝扦插苗生根过程中三个时期的水分管理,可供生产实践参考。 相似文献
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