Turbiscan Lab分散稳定性分析仪研究异丙甲草胺水乳剂物理稳定性

为获得不同因素对异丙甲草胺水乳剂物理稳定性的影响规律,同时验证Turbiscan Lab分散稳定性分析仪预测和评价水乳剂物理稳定性的可行性,利用Turbiscan Lab分散稳定性分析仪,结合加速试验[(54±2)℃,14d]结果,研究了乳化剂用量、体系pH和电解质离子等对磷酸酯盐稳定的异丙甲草胺水乳剂物理稳定性的影响。结果表明:乳化剂三苯乙烯基苯酚聚氧乙烯醚磷酸酯三乙醇胺盐(SCP)用量为6.0%(质量分数)时,水乳剂稳定性最好;过酸或过碱均会破坏水乳剂的稳定性,pH=9时水乳剂可获得较好的稳定性;Mg2+压缩油水界面双电层,导致水乳剂稳定性下降,且Mg2+添加浓度越大,水乳剂越不稳定。Turbiscan Lab分散稳定性分析仪以多重光散射为技术核心,在不破坏样品的前提下,能够准确测定乳状液中浮油、沉淀和液滴粒径大小的变化情况,与加速试验结果吻合度高,不仅能够缩短试验周期,而且可以分析不稳定现象发生的机理。     

Role of calreticulin-induced mitochondrial damage in high glucose-induced apoptosis of myocardial cells

AIM: To observe the effect of high glucose on the protein expression of calreticulin (CRT) and its association with cell apoptosis and mitochondrial dysfunction in the cardiomyocytes. METHODS: AC-16 cardiomyocytes were randomly divided into normal glucose group, high glucose group, high glucose+ CRT siRNA group and isotonic control group. The cell apoptotic rate, reactive oxygen species (ROS), mitochondrial membrane potential level, respiratory enzyme activity, and protein expression of CRT were observed. RESULTS: Compared with the cardiomyocytes in normal glucose group, the apoptotic rate and ROS production of cardiomyocytes increased in high glucose group, accompanying with the decreases in the mitochondrial membrane potential level and enzyme activitiy of the respiratory chain. The protein expression of CRT was significantly increased in high glucose group. However, compared with high glucose group, high glucose+ CRT siRNA decreased the expression of CRT and attenuated the damage of mitochondria, but CRT siRNA did not reduce the ROS level in cardiomyocytes. CONCLUSION: High glucose brings about CRT over-expression to induce mitochondrial injury, thus increasing myocardial apoptosis.     

Proteomic study of myocardial mitochondria with ischemia/reperfusion injury and pinacidil postconditioning in isolated rat hearts

AIM: To investigate the protective effect of pinacidil postconditioning on rat myocardium suffering ischemia/reperfusion injury by mitochondrial proteomics. METHODS: Langendorff apparatus was used to establish the model of myocardial ischemia/reperfusion injury. Sprague-Dawley rats were randomly divided into 2 groups: pinacidil postconditioning group (Pina group) and ischemia/reperfusion injury group (I/R group). After 20 min of perfusion with K-H solution, the perfusion was suspended for 40-min (global ischemia) follow by 60 min of reperfusion in I/R group. In Pina group at the end of 40 min global ischemia, the isolated hearts were perfused with K-H solution containing pinacidil (50 μmol/L) for 2 min followed 58-min perfusion with regular K-H solution. Total proteins extracted from the mitochondria were applied to the two-dimensional gel electrophoresis (2-DE). The differentially expressed protein spots over 2 times were evaluated by a software. Then they were subjected to in-gel digestion, and analyzed by spectrometry. RESULTS: The expression levels of NDUFA10, NDUFS2 and NDUFV2 were elevated but those of IDHA and ECH1 were decreased in Pina group compared with I/R group. Interestingly, 2 spots in the 2-DE map were identified as ATPase subunit δ. The expression levels of one spot was elevated, while the other was decreased. CONCLUSION: Pinacidil postconditioning may decrease the degree of increased expression levels of NDUFA10, NDUFS2 and NDUFV2, promote the expression of IDHA and ECH1, and induce the phosphorylation of ATPase subunit δ, which may be related to the protective mechanism of pinacidil postconditioning.     

金花菜新品种‘淮扬金花菜’

‘淮扬金花菜'是通过对江苏扬州、镇江等地种质资源调查并搜集整理,经多年筛选出的新品种,具有蛋白质含量高、品质好、产量高、再生性好、抗逆性强,综合性状优良的特点。粗蛋白含量29.41%,粗灰分含量7.94%,中性洗涤纤维含量21.71%,酸性洗涤纤维含量10.29%,适宜多次刈割,鲜草产量30 000 kg·hm~(-2)以上,适宜在长江中下游地区保护地或露地秋季种植。     

蒋卫杰博士：聚焦生产一线（九） 赤峰松山区日光温室早春茬菜豆高产高效 栽培技术

赤峰松山区现有日光温室菜豆生产面积1 067 hm²（1.6 万亩）,集中在松山区大庙镇
公主岭设施蔬菜园区,该园区经济效益高而远近闻名。其中早春茬菜豆上市时间处于北方蔬
菜生产淡季,每667 m² 收入4 万元以上,获得了较高的生产效益。     

柽柳红叶观赏新品种‘鲁柽3号’的选育

‘鲁柽3号’是通过选择育种方法选育出的红叶观赏新品种。该品种具有株型紧凑,当年生枝褐色有光泽,秋季叶色红至深红,观赏性强的特点,且耐重盐碱,是适宜北方盐碱地区种植的一个新品种。     

广西野生鳞盖红菇原种培养研究

为获得野生鳞盖红菇原种,利用通过组织分离获得的母种,根据母种分离培养经验并利用各种食用菌原种培养料搭配成多种配方培养红菇原种。结果表明:花生饼对原种培养有较大促进作用;麦子较适合作为培养原种主原料;配方棉籽壳15%、麦麸10%、麦子57%、玉米粉8%、花生饼8%、蔗糖0.8%、石膏1%、硫酸镁0.2%能长出较合格原种。     

五种杀螨剂对黄瓜茶黄螨的防治效果

以黄瓜上的茶黄螨为试材,采用田间试验的方法,研究了5种杀螨剂对大棚黄瓜茶黄螨的防治效果。结果表明:1.8%阿维菌素乳油3 000倍液的防治效果最好,1~14d防效均达93%以上;2.5%甲氨基阿维菌素苯甲酸盐微乳剂5 000倍液,具有较理想的防治效果,1~14d防效达90.60%~99.27%;43%联苯肼酯悬浮剂3 000倍液,药后1d的防治效果不太理想,防效只有73.26%,可是随着施药时间的延长,防治效果逐渐提高,药后7d和14d的防治效果达95%以上;240g/L螺螨酯悬浮剂4 000倍液和110g/L乙螨唑悬浮剂5 000倍液的防治效果相对较差;综合考虑,在保护地防治蔬菜上的茶黄螨,推荐使用1.8%阿维菌素乳油3 000倍液、2.5%甲氨基阿维菌素苯甲酸盐微乳剂5 000倍液,建议在茶黄螨发生初期使用43%联苯肼酯悬浮剂3 000倍液。     

1973-2000年黄河流域棉花品种改良与应用状况分析

利用1973-2000年国家黄河流域棉花区域试验资料及同期该棉区棉花品种推广的统计数据,总结了棉花品种改良的主要成绩,分析了棉花品种不同性状的育种效率.结果表明产量性状的遗传改良效率逐年下降,早熟性、抗逆性和部分纤维品质指标的改良效率有所提高.探索性提出了提高育种效率的途径和方法.分析了棉花良种普及率及自育品种变化情况,针对目前美国转基因棉花在该区生产中优势,加强国产品种的推广已成当务之急.     

赤峰地区玉米主推品种基础种质的系谱分析

作者利用生态育种的观点,通过对赤峰地区历史上玉米主推品种亲本自交系来源进行系谱分析,从中找出适应本生态区域生态条件下相适应的基础种质,为赤峰玉米育种从基础材料上找到一条简便可行的途径,同时也为赤峰玉米育种制定具体目标时提供了许多信息技术参数.