绿头野鸭与我国主要地方鸭品种遗传多样性研究

利用17个微卫星标记,采用PCR扩增,8%非变性聚丙烯酰胺凝胶电泳、银染,对绿头野鸭种群和我国9个主要地方鸭品种的遗传多样性进行了检测,统计了各群体的平均有效等位基因(Ne)、平均遗传杂合度(H)、多态信息含量(PIC)、群体间的遗传距离(Ds)和相似系数(I)。采用UPGMA和NJ法构建系统发生树。结果表明:17个微卫星座位在绿头野鸭种群和我国9个主要地方鸭品种中的多态信息含量均为高度多态,所有鸭种的平均PIC、H、Ne分别为0.6506、0.6896、4.1±1.5,基因多态性和遗传多样性相对较高;家鸭各品种间的遗传距离在0.1631~0.6844范围内,绿头野鸭与家鸭品种间的遗传距离分别在0.3096~0.8219之间。根据系统发育树推断中国主要地方鸭品种不是单一起源于绿头野鸭。中国主要地方鸭品种大致可以分为5支。一支为北京鸭、荆江鸭,一支为中山鸭、建昌鸭及高邮鸭,一支为靖西大麻鸭和金定鸭,连城鸭为一支,绍兴鸭为另一支。     

Real-time PCR方法和PCR方法检测虾白斑综合征病毒

由白斑综合征病毒引起的虾白斑综合征是国际兽医局（OIE）规定的必须上报的水生动物二类疫病。本研究首先通过设计新的PCR引物，提高了PCR检测白斑综合征病毒的阳性检出率。为进一步提高白斑综合征病毒检测的灵敏度，本研究采用TaqMan探针技术建立了快速检测白斑综合征病毒的real-time PCR方法，通过与常规PCR方法比较，证实其检测灵敏度显著提高。     

Study on the immunological function of sodium butyrate-induced immature human monocyte-derived dendritic cells

AIM: To investigate the immunological function of sodium butyrate-induced immature dendritic cells in vitro.METHODS: The human monocyte-derived dendritic cells were induced in the presence of human granulocyte macrophage-colony stimulating factor(GM-CSF) and interleukin-4 (IL-4), combined with sodium butyrate. The immunological function of sodium butyrate-induced dendritic cells was detected by the FCM, endocytic activity, T cells stimulatory proliferation capacity, and interleukin-12 (IL-12) production.RESULTS: Sodium butyrate could down-regulate the major histocompatibility complex(MHC) class II and costimulatory molecules of dendritic cells, increase the endocytic activity, induce a stage of T-cell anergey, and inhibit the T helper cell type 1-skewing factor IL-12 production. CONCLUSION: Sodium butyrate inhibits the maturation of dendritic cells and induces production of immature dendritic cells, which may help to explore the machenism of its epigenitic modification.     

家蚕微孢子虫(浙江株)α-微管蛋白基因部分片段的克隆及系统发育分析

基于微孢子虫分类学地位的争议,通过克隆家蚕微孢子虫(Nosema bombycis)的α-Tubulin基因,利用BioEditor将核苷酸序列翻译成氨基酸序列,并从NCBI中收集不同物种的α-Tubulin基因,用CLUSTALX(1.81)、Mega2以及在线生物信息学软件CLUSTALW(1.83)分析序列并构建系统发育树。结果显示:家蚕微孢子虫及其它微孢子虫与真菌聚为一类,且微孢子虫以一个独立群与接合菌(zygomycetes)的噬虫霉属(Entomophaga)、耳霉属(Conidiobolus)关系最近,与子囊菌(ascomycetes)、担子菌(basidiomycetes)、壶菌(chytrids)及其它接合菌互为姐妹群。     

气候变化对陇东冬小麦生态影响特征研究

对黄土高原比较有典型代表性的残塬"董志塬"的变暖及冬小麦生态响应特征进行研究,发现"董志塬"近35 a来年平均增温线性趋势达0.0505℃,增温表现出平均气温、最高气温、最低气温同时上升,且冬春季增温速度最快,秋季次之,夏季增温最平缓;增温对冬小麦的生态影响主要体现在全生育期(线性趋势0.91 d/a)、越冬期(线性趋势0.85 d/a)显著缩短,春季发育期普遍提前(返青期以0.57 d/a的线性趋势提前,拔节期以0.42 d/a的线性趋势提前,成熟期以0.48 d/a的线性趋势提前),而春季各发育期间间隔日数并未出现缩短的趋势.认为气候变暖对当地农业生产有利有弊,气候变暖,尤其冬春气温显著升高将导致越冬期土壤水分损耗增大,春旱加剧,不利于春季农业生产;另一方面冬小麦全生育期缩短,成熟期提前,能有效提高复种指数和土地利用率.     

污水灌溉对冬小麦产量及其品质的影响

通过测桶试验,研究了污水、稀释污水、再生水和清水灌溉条件下,冬小麦籽粒中淀粉、蛋白质、氮、磷、钾含量的变化,以及不同水质灌溉对小麦籽粒产量和籽粒中重金属残留的影响.结果表明:当灌水定额相同时,4种不同水质处理,小麦籽粒中粗蛋白质的含量为污水＞稀释污水＞再生水＞清水,且污水中粗蛋白质的含量要明显高于其他水质处理;低灌水定额时,污水、稀释污水和再生水处理与对照相比分别增产12.71%,9.57%和1.14%;高灌水定额时,污水、稀释污水和再生水处理与对照相比分别增产12.22%、5.05%和2.12%.污水、稀释污水和再生水与清水相比,小麦籽粒中的重金属含量无显著差异,其单项污染指数和综合污染指教远小于1.未形成污染.     

我国灌区农业水资源可持续利用问题探讨

本文分析我国灌区农业水资源利用存在的主要问题是水资源短缺与水资源浪费共存、现行体制和政策难以促进有效的节水机制形成、灌溉工程老化、设施薄弱、水资源不合理利用导致生态环境恶化；论述了水价政策对灌区农业水资源可持续利用的影响，提出改革水价以促进灌区节水农业发展的思路；探讨了我国节水农业发展现状与存在的主要问题是节水农业技术不普及，理论认识上存在偏差，注重单项技术，缺乏深入的节水技术综合集成，节水农业创新水平不高，农业节水投资力度不足；提出了我国灌区农业水资源可持续利用的出路是树立可持续利用观念、大力发展节水农业，改革现有管理体制，建立高效有序的水资源管理模式，拉动经济杠杆，建立节水灌溉经济激励机制，建立用户参与管理决策的民主管理机制，建立科学的水价体系，促进地表、地下及降水联调，加强宣传和教育，充分认识水资源危机的紧迫性，为水价改革奠定舆论基础。     

棉铃虫齿唇姬蜂触角感器的扫描电镜观察

棉铃虫齿唇姬蜂雌雄蜂触角上感器的扫描电镜观察结果显示，共发现毛形感器、板形感器、钟形感器、锥形感器、Bohm氏鬃毛和刺形感器6类感器，分别对这6类感器的类型与形态进行了详细描述，并对各种感器的功能进行了深入的分析和讨论。     

切轴流式双滚筒大豆种子脱粒机设计与试验

为解决大豆种子脱粒损伤率高和脱净率低的矛盾,提出了钉齿式副滚筒切流预脱、弓齿与钉齿相间组合排列的主滚筒轴流脱粒、切轴流式双滚筒组合脱粒方案,进行了脱粒关键部件结构与参数设计,采用直径较小而短的副滚筒完成大豆植株的打击、抓取和拖带等切流预脱,主脱粒滚筒与副滚筒同向等速且轴向长度和直径均较大,由弓齿与钉齿组合而成,进行大豆的轴流脱粒;设计了样机并进行了脱粒性能试验。采用二次回归正交旋转中心组合优化试验方法,分别建立大豆脱粒损伤率、未脱净率与喂入量、主滚筒转速和主滚筒脱粒间隙关系的回归数学模型,利用Design-Expert 8.0软件对该模型进行优化求解得到最佳参数组合,试验结果表明:在大豆籽粒含水率为17%~19%、秸秆含水率为12%~15%、大豆草谷比1.275条件下,当喂入量为0.44 kg/s、主滚筒转速为489 r/min、主滚筒脱粒间隙为25.06 mm时,大豆脱粒损伤率为1.18%、未脱净率为0.65%;与传统大豆脱粒机相比可使脱粒损伤率和未脱净率分别降低0.22个百分点和0.38个百分点。     

Repressive effect of miRNA-363 via SOX4 on human osteosarcoma cell growth and apoptosis

AIM: To detect the expression of miRNA-363 and SOX4 in osteosarcoma tissues and to investigate the effect of miRNA-363 on the viability and apoptosis of human osteosarcoma cell line MG-63.METHODS: Real-time PCR was used to detect the expression level and the relationship of miRNA-363 and SOX4 mRNA in the osteosarcoma tissues and the corresponding paratumorous tissues collected from 63 patients. The expression levels of miRNA-363 and SOX4 in osteosarcoma cell line MG-63 after transfected with miRNA-363 mimics were measured. The cell viability was measured by CCK-8 assay. Flow cytometry was used to monitor the changes of cell cycle and apoptosis. The changes of SOX4 and miRNA-363 expression levels in the MG-63 cells after transfection with SOX4 siRNA or pcDNA/SOX4 was detect by real-time PCR.RESULTS: The expressed level of miRNA-363 was lower, and the expression level of SOX4 was higher in the osteosarcoma tissues than those in the adjacent normal tissues. A significantly negative correlation between the expression levels of miRNA-363 and SOX4 was observed. The expression of miRNA-363 in the MG-63 cells after transfection with miRNA-363 mimics was significantly up-regulated, while the expression of SOX4 in the MG-63 cells was significantly down-regulated, with significant difference as compared with the cells transfected with miRNA-NC and control cells. The viability of MG-63 cells was inhibited, the cell cycle was arrested in G₀/G₁ phase, and the cell apoptosis was increased by transfection with miRNA-363 mimics. The relative protein expression levels of SOX4 in SOX4 siRNA group and pcNDA/SOX4 group were significantly different from those in negative control group, but the relative expression levels of miRNA-363 had no significant difference. Over-expression of SOX4 restored the viability of the MG-63 cells reduced by miR-363.CONCLUSION: The expression level of miRNA-363 is low in human osteosarcoma tissue. miRNA-363 may inhibits the viability of osteosarcoma cell line MG-63 and promotes cell apoptosis in vitro via inhibiting the SOX4 expression.