我国体育经纪组织发展困境及破解路径分析

以文献研究、逻辑分析为主要研究方法，分析了当前我国体育经纪组织发展所面临的困境，指出了我国体育经纪组织品牌化发展的必要性。从五个方面提出了我国体育经纪组织品牌化发展的思路：增强体育经纪组织品牌意识、实施体育经纪组织品牌战略、设计体育经纪组织品牌要素、塑造体育经纪组织品牌文化、打造体育经纪组织知名品牌。     

某增压器的整机动不平衡量偏大的工艺改进

通过对某四缸柴油机用增压器的整机动平衡通过率低的原因分析,找到影响整机动平衡的主要原因,通过工艺参数的调整,使整机动平衡达到设计要求.     

Repression of SFRP5 promoter activity by hepatitis B virus X protein

AIM: To investigate the molecular mechanism that hepatitis B virus X protein (HBx) inhibits the promoter activity of secreted frizzled-related protein 5 (SFRP5). METHODS: Serial truncated fragments of SFRP5 promoter were amplified and cloned into luciferase reporter vector pGL3-Basic. LO2 cells were transfected with recombinants, and then infected with the adenoviral vector expressing HBx protein (Ad-HBx) or the analogous adenovirus expressing green fluorescent protein (Ad-GFP) for control. The infection efficiency was examined under a fluorescence microscope and the activity of SFRP5 promoter was measured by luciferase assay. RESULTS: Truncated fragments of SFRP5 promoter were successfully constructed. Compared with the pGL3-Basic control, the luciferase activity was 6.32±0.04 of the -478 bp~+47 bp fragment, 5.79±0.32 of the -811 bp~+47 bp fragment, 3.59±0.34 of the -1 235 bp~+47 bp fragment, 3.86±0.39 of the -1 677 bp~+47 bp fragment and 3.26±0.42 of the -2 072 bp~+47 bp fragment, respectively. Overexpression of HBx inhibited the activity of SFRP5 promoter. The average inhibitory rates were 44% for -478 bp~+47b, 46% for -811 bp~+47 bp, 28% for -1 235 bp~+47 bp, 24% for -1 677 bp~+47 bp and 40% for -2 072 bp~+47 bp, respectively. CONCLUSION: HBx suppresses the activity of SFRP5 promoter, leading to down-regulation of SFRP5 expression.     

云计算在中小企业信息化中的应用研究

云计算是继网格计算后又一项正在兴起中的技术,它的出现使"互联网上众多的计算机成为一台虚拟超级计算机"的梦想逐渐变成了现实。概述了云计算的概念与原理,阐明了云计算在中小企业中实施的技术与优势,提出了云计算在中小企业实施中存在的问题以及解决问题的策略。     

信阳市苗木生产中存在的问题与对策

信阳市苗木生产发展迅速,但仍存在着品种单一、结构不合理、缺少品牌优势、科技含量低等问题。应采取统一规划、合理种植、因地制宜等技术措施,促进信阳市苗木生产的可持续发展。     

关于温州市推行水稻机械栽插的思考

文章首先分析了浙江省温州市机插的现状,指出加快突破插秧机械化技术瓶颈,已成为推进水稻生产全程机械化,实现农业现代化的重要主攻方向,要制订切实可行的水稻插秧机械化推进计划;从节省秧田、节水肥药、节本省工、高产稳产、避灾增效、五个方面阐述了机插的优势,从技术上可行、政策上有利、推广有依托、多数农民观念转变四个方面阐述了机插有利条件;最后提出推进水稻机插的对策与措施。     

单边支重轮半体切边-冲孔复合模设计

介绍了在曲柄压力机上切边冲孔的复合模结构设计、工作原理及装配和调试要求等.     

Design Method of Moment-strengthening on Tension Side When Load Un removed

Based on the linear strain distribution assumption, strain development of tensile bars and ultimate moment capacity of strengthening section, which strengthened on tension side with service load un removed, is investigated. It is showed that the higher the service load bending moment to original moment capacity ratio M 1k /M u1 is, the smaller the tensile strain of strengthening re barsis, and the more the amount of strengthening re barsis, the smaller the tensile strain of original re barsis. It also showed that,When original re bar area is relatively small and strengthening re bar area is adequate, both original and strengthening re bars may get yielded and a great increment in section moment capacity is achieved, even though the ration M 1k M u1 has a relatively high value.     

退耕还林还草中建立生态系统与经济系统“弹性资源”初论

退耕还林还草中．建设生态系统与经济系统“弹性资源”,对于良性生态经济系统调控机制的形成具有重要的作用。通过对其概念的界定．得出了生态系统与经济系统“弹性资源”的3个重要特征：(1)具有多种可供利用的途径．可满足不同阶段生态环境建设和商品生产发展的需求;(2)具有稳定的生态经济系统结构和良好的自我调控机制;(3)满足生态目标、经济目标和社会目标的有机统一。以县南沟示范区为例研究了其弹性资源建设标准和进展。     

山羊卵母细胞无血清体外成熟培养

为研究无血清培养系统对山羊卵母细胞体外成熟及孤雌激活胚胎早期发育力的影响,分别以0.3%牛血清蛋白(BSA)(mV)、1%聚乙烯醇(PVA)(mV)和1%胰岛素转铁蛋白亚硒酸钠(ITS)(VV)代替成熟培养液中胎牛血清(FBS),以添加5?S的OM为对照组,对来源于屠宰场的卵巢卵母细胞复合体(COCs)进行体外培养。成熟率PVA添加组(50.00%)显著低于FBS组(83.23%)(P<0.01)、BSA组(71.19%)和ITS组(76.79%),与FBS组无显著差异(P>0.05);成熟卵母细胞用离子霉素联合6-DMAP激活,在SOFaa中进行孤雌胚培养,BSA、PVA、ITS及FBS各组的卵裂率依次为61.90%、46.97%、79.07%和83.58%,囊胚率分别为25.00%、19.70%、55.81%和60.45%。结果表明:在OM基础培养液中添加BSA或PVA,卵母细胞的成熟率明显低于添加FBS,且卵裂率和囊胚发育率也低于对照组(P<0.05或P<0.01);添加ITS,卵母细胞成熟率虽低于对照组,但孤雌胚卵裂率和囊胚发育率与对照组接近(P>0.05)。表明以ITS代替FBS,可用于山羊卵母细胞体外成熟培养。