牛miR-144靶基因预测与组织表达分析

为研究miR-144的靶基因与miR-144在牛不同组织中的表达规律,预测其对肌肉生长发育的调节机制,对miR-144在各物种间的保守性、潜在靶基因及其富集通路进行分析,通过qRT-PCR方法检测牛不同组织中miR-144的表达.结果表明,miR-144成熟序列在各物种间保守性较高;富集分析发现,靶基因显著富集于血管平...     

颗粒体积浓度对半开式叶轮离心泵泄漏涡和磨损的影响

固液两相流条件下半开式叶轮离心泵中颗粒冲击、泄漏涡发展和颗粒轨迹之间存在紧密交互作用,导致过流部件的磨损行为复杂多变。该研究结合双向耦合欧拉-拉格朗日方法和颗粒磨损Finnie模型,对不同颗粒体积浓度下半开式叶轮离心泵固液两相流场进行求解,分析了颗粒体积浓度对泄漏涡结构特征、颗粒运移轨迹和磨损特性的影响,揭示了颗粒体积浓度、叶顶间隙泄漏涡和过流部件表面磨损规律的关联机制。结果表明：随着颗粒体积浓度的增加,颗粒的频繁撞击加剧了叶片压力面进水边和后盖板磨损程度,叶片吸力面出水边的磨损范围向进水边方向延伸;颗粒体积浓度小于1%时,颗粒的轴向运动和叶顶间隙泄漏涡的阻碍作用导致颗粒易与叶片前缘靠近叶根处和吸力面出水边靠近叶顶的区域发生撞击,诱发严重磨损,且呈现点状磨损;当颗粒体积浓度大于3%时,叶轮后盖板的整体磨损强度大于叶片,颗粒体积浓度的增加造成流入叶顶间隙层的颗粒数增加,颗粒对叶顶间隙泄漏涡的冲击导致涡流的破碎、分离、再融合,加剧不稳定流动,泵的扬程和效率均明显下降。该研究可为固液两相半开式叶轮离心泵优化设计和安全稳定运行提供理论参考。     

1990-2020年天津市植被覆盖度时空演变特征及影响因素分析

植被覆盖度(FVC)是区域生态环境质量的重要参数,揭示长时序FVC时空分异特征及识别主导影响因子可为评估地表植被状况提供科学参考。以天津市为例,基于Google Earth Engine(GEE)云平台的1990—2020年Landsat遥感影像,结合像元二分模型计算FVC。运用一元线性回归、F检验进行FVC时空演变趋势分析,利用变异系数、Hurst指数分析FVC波动情况及未来变化趋势。在此基础上,应用随机森林(RF)模型的特征重要性排序、偏依赖图技术量化FVC与同期的气候因素(年均气温、年降水量)和人类活动因素(土地利用、人口密度)的非线性响应关系,并进一步分析多因素耦合对FVC空间分布的解释力度。结果表明：(1)在时间上,1990—2020年天津市FVC呈显著下降趋势,变化率为0.001 5(p<0.05),其中1990—2012年FVC显著降低,2012年后变化趋势不显著(p>0.05)。不同等级FVC时序差异明显,低、中低和中植被覆盖比例均呈现显著增加趋势(p<0.05),而中高和高植被覆盖比例一致表现为显著降低(p<0.05)。(2)在空间上,1990...     

Seroepidemiological survey of distemper virus infection in the Caspian Sea and in Lake Baikal

Forty Caspian seals were surveyed seroepidemiologically between 1993 and 1998 around the times of mass mortality that occurred in 1997 in the Caspian Sea and seven Baikal seals were also surveyed in 1998. Virus neutralizing tests and ELISA clearly suggested that distemper virus epidemic was caused in Caspian seals before the spring of 1997 and that CDV infection continued to occur in Lake Baikal in recent years.     

HPLC-PDA法测定兽用中药液体制剂中非法添加的四种解热镇痛类药物

实验建立了兽用中药液体制剂中非法添加对乙酰氨基酚、安乃近、氨基比林、安替比林四种解热镇痛类药物的高效液相色谱-二极管阵列(HPLC-PDA)检测方法。方法采用CAPCELL PAK MGⅡS5 C18(4.6 mm×150 mm,5μm)色谱柱,柱温30℃,流动相为磷酸盐缓冲液和乙腈(84:16),流速1.0 mL/min,进样体积10μL,采集波长范围为190~400 nm,检测波长229 nm。结果显示,对乙酰氨基酚、安乃近、安替比林、氨基比林在浓度分别在1.17~117.28、2.03~202.92、5.58~111.60、2.42~120.80μg/mL范围内线性良好,相关系数R 2分别为0.9986、0.9999、0.9980、0.9993,平均加标回收率(n=5)分别为93.15%~104.25%、93.79%~104.62%、96.96%~99.17%、94.66%~99.83%,RSD分别为0.46%~2.29%、0.30%~3.15%、0.12%~0.94%、0.37%~1.42%(n=5)。该方法便捷,快速,准确,可用于兽用中药液体制剂中非法添加四类解热镇痛类药物的筛查检测。     

不同砧木对普通型和短枝型 '富士'苹果树体生长生理特性和果实品质的影响

[目的]探究不同砧木对普通型和短枝型"富士"苹果树生长和结果的影响,为千阳区域筛选适宜的砧穗组合给予借鉴.[方法]以'长富2号'和'礼泉短富'搭配不同栽培类型的砧木为研究对象,测定其树体生长势、果实品质以及产量等生理指标,同时借助模糊数学MF(隶属函数)法全面评估4个品种的果实品质.[结果]在千阳区域的矮化高密度种植环...     

结球甘蓝抽薹开花时间性状的 QTL 定位及分析

在已构建的结球甘蓝AFLP、SSR和SRAP标记高密度遗传图谱的基础上,运用MapQTL 4．0软件对结球甘蓝F2群体抽薹、开花时间两性状分别进行QTL定位和分析。最终检测到2个控制甘蓝抽薹时间性状的QTL（ qbt-3-2、qbt-9-1）,以及一个同开花时间性状相关的QTL（qft-9-1）,分别位于连锁群LG3与LG9上,这3个QTL均为增效位点,共解释甘蓝抽薹、开花时间性状变异的17．5％,22．7％;同时得到与QTLs共分离的2个分子标记E46 M52-5、me26-em13-1,均可作为辅助选育甘蓝耐抽薹品种的标记使用,这将为结球甘蓝耐抽薹品种的选育提供理论依据。     

汞胁迫对大豆幼苗SOD和CAT活性的影响

采用营养液培养方法,以不同Hg2+浓度处理大豆,测定大豆种子萌发情况及其幼苗中超氧化歧化酶(SOD)和过氧化氢酶(CAT)活性.结果表明:在不同Hg2+浓度范围(0 ～200.0 mg/L)内,随着Hg2+浓度不断升高,大豆种子发芽率逐渐下降,在不含Hg2+溶液内,大豆种子发芽率最高达到100％,Hg2+浓度在200.0 mg/L时大豆种子萌发率为0;在Hg2+浓度胁迫下,低Hg2+浓度胁迫促使植株生长率上升,而随着Hg2+浓度升高,大豆植株的生长率逐渐下降,大豆植株生长受到抑制;当胁迫浓度增加,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)呈先上升后下降的趋势;Hg2+对大豆幼苗生长抑制程度与处理浓度呈正相关性.     

Performance of Polyepoxysuccinic Acid(PESA) in the Biological Treatment of High Hardness Wastewater

The settling property, COD removal rate, Ca 2+ retention rate, variations of biofacies and the effect of sludge reduction of the high hardness wastewater of the activated sludge process by adding green and safe scale inhibitor PESA (Polyepoxysuccinic Acid) during the experiment have been studied for the COD was 100～300 mg/L, calcium concentration was 110～280 mg/L of the high hardness of wastewater. The results show that MLSS was less than the ordinary activated sludge reactor, the content of the inorganic substrate in activated sludge was significantly reduced and resulted in the decrease of calcium activated sludge putout, the reduction reached to 41.33%, The purpose of calcium wastewater treatment sludge reduction was achieved.The COD removal rate of PESA activated sludge reactor reached above 90%, the Ca 2+ retention rate reached above 80%. PESA inhibits the sedimentation of a part of calcium salt in activated sludge surface, bioactivity was kept well, and in this concentration range, indicative microbial were able to grow and reproduce. The ability to resist impact load has been enhanced in high hardness water, sludge age extension.     

Viscoelastic Model Analysis of In-situ Pressuremter Creep Test in Permafrost

In order to investigate the creep behavior of warm and ice-rich frozen soils, a number of pressuremeter tests were carried out in permafrost regions on the Qinghai-Tibetan Plateau with Menard pressuremeter. Then, the regression analysis results of the creep curves generated by Merchant model and relevant parameters were obtained. The test results indicate that the temperature plays a more important role than the water content in affecting the mechanical behavior of warm frozen soils. As pressure increases, the proportion of instantaneous strain in the total strain under each pressure decreases gradually. The regression results show that the instantaneous shear modulus increases linearly with decreasing temperature, but the delayed shear modulus as well as the viscous coefficient present a power function trend. Their peak values appear at the water content of 46%, which is rather different from the test result on lower temperature frozen soils.