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21.
M F Borgerding R D Hicks J E Bodnar D M Riggs E J Nanni G W Fulp W C Hamlin J A Giles 《Journal of the Association of Official Analytical Chemists》1990,73(4):605-609
The design of a new cigarette that heats rather than burns tobacco calls for modifications to the Federal Trade Commission (FTC) method for analytical smoking. These changes include eliminating sample conditioning at 75 degrees F and 60% RH, exercising greater care in lighting cigarettes, and smoking cigarettes to self-extinguishment rather than to a predetermined butt length as a measure of complete consumption. By several gross analytical measures, smoke condensate from the new cigarette differs substantially from that of tobacco-burning cigarettes. This is inferred from the lack of coloration of smoke condensate collected on Cambridge filters. Elemental analysis demonstrates reduced carbon and nitrogen content concurrent with increased hydrogen. Thermogravimetric analysis shows almost quantitative weight loss at Tmax = 220 degrees C. Ultraviolet (UV) spectrophotometric analysis shows greatly reduced levels of tobacco-derived smoke components and qualitative differences in chemical entities being measured. By design, the heat required for smoke formation is supplied by a carbon heat source embedded in the cigarette tip. Tobacco contained in the cigarette is not burned and is exposed to temperature less than 300 degrees C. Thus, it is apparent (1) that smoke from the new cigarette contains little or no "tar" as tar is classically defined, and (2) that the FTC method even as modified to account for cigarette design differences is appropriate only for determination of nicotine and carbon monoxide yielded from this cigarette. 相似文献
22.
Archaeologists often encounter problems in interpreting the genesis of layers and the context in which specific materials occur in excavations. Field visits of geologists, soil scientists or biologists can solve a number of questions, but often, problems remain that only can be studied by microscopic research as, for example, soil micromorphology and palynology. In the past, the employment of these two disciplines, one in earth science and one in biology, generally occurred independently of each other, also in interdisciplinary research and contract research. Consequently, results could not always be correlated, permitting only a restricted integration of data. It was realised that soils, as archives, contain much more information than generally utilised. A skillful employment and balancing of both specialties create a real integrated approach that offers a substantially improved interpretation potential without hardly additional efforts and costs. The results of 7 years of experience were very stimulating and presented here.In this article, the essence of soil micromorphology and palynology applied to archaeology is briefly outlined and illustrated by selected examples of the different research themes covered, showing the possibilities and results of integrated research. Both specialties generate essential information that, when combined and integrated properly, lead to improved understanding of the landscape genesis and human activities in the past. To be able to employ the full interpretive potential of this integrated approach, the research procedures followed by both specialties had to be compared. The key requirements for optimal integration of each other's databases comprised a combined effort to compare the pros and cons during the first four steps of the research procedure: defining research questions, field survey, selection of sampling locations and sampling. This approach enabled optimal correlation of the individual results, made cross-checking and references possible and led to higher levels of interpretation that would not have been possible otherwise. The integrated research elucidated lacunae in the other's discipline, resulting in a distinctly more complete reconstruction of paleoenvironments and past land uses, important research themes of archaeologists for the specialties concerned. 相似文献
23.
Jen JC Chan WM Bosley TM Wan J Carr JR Rüb U Shattuck D Salamon G Kudo LC Ou J Lin DD Salih MA Kansu T Al Dhalaan H Al Zayed Z MacDonald DB Stigsby B Plaitakis A Dretakis EK Gottlob I Pieh C Traboulsi EI Wang Q Wang L Andrews C Yamada K Demer JL Karim S Alger JR Geschwind DH Deller T Sicotte NL Nelson SF Baloh RW Engle EC 《Science (New York, N.Y.)》2004,304(5676):1509-1513
The mechanisms controlling axon guidance are of fundamental importance in understanding brain development. Growing corticospinal and somatosensory axons cross the midline in the medulla to reach their targets and thus form the basis of contralateral motor control and sensory input. The motor and sensory projections appeared uncrossed in patients with horizontal gaze palsy with progressive scoliosis (HGPPS). In patients affected with HGPPS, we identified mutations in the ROBO3 gene, which shares homology with roundabout genes important in axon guidance in developing Drosophila, zebrafish, and mouse. Like its murine homolog Rig1/Robo3, but unlike other Robo proteins, ROBO3 is required for hindbrain axon midline crossing. 相似文献
24.
In vitro studies have indicated that reactive oxygen species (ROS) and the oxidation of signaling molecules are important mediators of signal transduction. We have identified two pathways by which the altered redox chemistry of the clk-1 mutants of Caenorhabditis elegans acts in vivo on germline development. One pathway depends on the oxidation of an analog of vertebrate low density lipoprotein (LDL) and acts on the germline through the Ack-related tyrosine kinase (ARK-1) kinase and inositol trisphosphate (IP3) signaling. The other pathway is the oncogenic ras signaling pathway, whose action on germline as well as vulval development appears to be modulated by cytoplasmic ROS. 相似文献
25.
26.
Roberson ED Scearce-Levie K Palop JJ Yan F Cheng IH Wu T Gerstein H Yu GQ Mucke L 《Science (New York, N.Y.)》2007,316(5825):750-754
Many potential treatments for Alzheimer's disease target amyloid-beta peptides (Abeta), which are widely presumed to cause the disease. The microtubule-associated protein tau is also involved in the disease, but it is unclear whether treatments aimed at tau could block Abeta-induced cognitive impairments. Here, we found that reducing endogenous tau levels prevented behavioral deficits in transgenic mice expressing human amyloid precursor protein, without altering their high Abeta levels. Tau reduction also protected both transgenic and nontransgenic mice against excitotoxicity. Thus, tau reduction can block Abeta- and excitotoxin-induced neuronal dysfunction and may represent an effective strategy for treating Alzheimer's disease and related conditions. 相似文献
27.
Unlike mercury, which has an extensive +1 oxidation state chemistry, zinc usually adopts the +2 oxidation state. Decamethyldizincocene, Zn2(eta5-C5Me5)2, an organometallic compound of Zn(I) formally derived from the dimetallic [Zn-Zn]2+ unit, has been isolated from the low-temperature (-10 degrees C) reaction of Zn(C5Me5)2 and Zn(C2H5)2 in diethyl ether. X-ray studies show that it contains two eclipsed Zn(eta5-C5Me5) fragments with a Zn-Zn distance (+/- standard deviation) of 2.305(+/-3) angstroms, indicative of a metal-metal bonding interaction. 相似文献
28.
The reproductive organs of some plants self-heat, release scent, and attract pollinators. The relations among these processes are not well understood, especially in the more ancient, nonflowering gymnosperm lineages. We describe the influence of plant volatiles in an obligate pollination mutualism between an Australian Macrozamia cycad (a gymnosperm with male and female individuals) and its specialist thrips pollinator, Cycadothrips chadwicki. Pollen-laden thrips leave male cycad cones en masse during the daily thermogenic phase, when cone temperatures and volatile emissions increase dramatically and thrips are repelled. As thermogenesis declines, total volatile emissions diminish and cones attract thrips, resulting in pollination of female cones. Behavioral and electrophysiological tests on thrips reveal that variations in b-myrcene and ocimene emissions by male and female cones are sufficient to explain the observed sequential thrips' repellence (push) and attraction (pull). These dynamic interactions represent complex adaptations that enhance the likelihood of pollination and may reflect an intermediate state in the evolution of biotic pollination. 相似文献
29.
J. Jesús Casas Juan S. Sánchez-Oliver Ana Sanz Miriam Furné Cristina Trenzado Melchor Juan Mariano Paracuellos María D. Suárez Francisca Fuentes Irene Gallego Carlos Gil José J. Ramos-Miras 《Biological conservation》2011,(1):253-262
We studied the relative value of natural habitats, river and coastal wetlands, and artificial habitats, irrigation canal and ponds, for the conservation of an endangered fish, Iberian toothcarp, in its southernmost area of distribution, characterised by agricultural intensification. Our results show that the bulk of the population of the Iberian toothcarp is concentrated in irrigation ponds. Natural habitats sustained null or impoverished subpopulations, and individuals showed signs of low metabolic activity. This coincided with the relatively high habitat quality observed in ponds, particularly those with submerged aquatic vegetation, in contrast with the chronic eutrophication of the coastal lagoons. In spite of a generalised aggressive management in the irrigation system, featured by periodic vegetation clearance, desiccation and biocide treatment, the subpopulation of the Iberian toothcarp thrives in it probably thanks to adequate water quality and to an active path dynamics maintained by connectivity through the canal. Agro-environmental measures are discussed for the improvement of this species conservation in natural and artificial habitats. 相似文献
30.
Barbosa J Freitas A Moura S Mourão JL Noronha da Silveira MI Ramos F 《Journal of agricultural and food chemistry》2011,59(22):11927-11934
Nitrofurans were broadly used as an extremely effective veterinary antibiotic especially in pig and poultry production farms. Because of fears of the carcinogenic effects on humans, the nitrofurans were banned from use in livestock production in many countries, including the European Union. The present study examines the accumulation, distribution, and depletion of furaltadone and nifursol and of their tissue-bound metabolites [3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) and 3,5-dinitro-salicylic acid hydrazine (DNSAH), respectively, in poultry edible tissues (muscle, liver, and gizzards) following administration to chickens of therapeutic and subtherapeutic concentrations of both compounds. Nitrofurans determination was performed by high-performance liquid chromatography-diode array detection and liquid chromatography-tandem mass spectrometry, respectively, for feeds and for poultry tissues. Furaltadone and nifursol, in very low concentrations, were found in samples of muscle, liver, and chicken's gizzard collected from slaughtered animals after 5 weeks of treatment and no withdrawal time period. When a withdrawal time period of 3 weeks was respected, no detectable nitrofuran parent compounds was observed in all of the studied matrices. For AMOZ, concentrations of 270 μg/kg in meat, 80 μg/kg in liver, and 331 μg/kg in gizzard were determined after administration of a medicated feed with furaltadone (132 mg/kg), 3 weeks after withdrawal of treatment. For DNSAH, the concentration values obtained are much lower than those observed for AMOZ. For meat, liver, and gizzard, DNSAH concentrations of 2.5, 6.4, and 10.3 μg/kg, respectively, were determined, after administration of a medicated feed with nifursol (98 mg/kg), 3 weeks after withdrawal of treatment. The gizzard could be considered a selected matrix for nitrofuran residues evaluation in poultry, due to its capacity of retaining either nitrofuran parent compounds or metabolites in higher concentrations, regardless of the administered dose or of the respected withdrawal time period. 相似文献