黑龙江省主要阔叶树种绿枝扦插试验

在带岭林科所落叶松种子园大棚内进行了10个树种、3个采条时期、3种插穗处理和15种基质1350个组合的绿枝扦插试验。结果表明，同一树种的不同采条时期、不同插穗处理、不同基质和不同树种的同一采条时期、同一插穗处理、同一基质的生根效果不同，并有明显的交互作用。各树种最佳组合的生根率：刺五加80％、五味子70％、紫椴40％、花楸30％、水曲柳20％、黄菠萝20％、山槐20％、山梨10％、春榆10％、胡桃楸0。     

电磁屏蔽功能胶合板初探

为了赋予木基复合材料电磁屏蔽功能,在脲醛树脂胶中加入碳、石墨等导电单元,制备3层结构的杨木胶合板,研究不同导电单元对胶合板电磁屏蔽效能的影响.结果表明:导电单元的粒度越小,胶合板的电磁屏蔽效能越好,最大可达22 dB,但相关机理和量化关系还有待于深入研究.     

用意杨压缩木替代传统木梳材料的研究

我国木梳素以小叶黄杨、楠木、枣木、梨木等树种为制梳材料。由于人口膨胀,木梳需求量巨增,制梳原材料渐已匮乏,绵延1 500多年的传统木梳手工艺文化正面临消亡的威胁。笔者选择速生树种——意杨为制梳材料,按木梳实用功能和产业经济的实际需求,对意杨压缩木进行一般物理特性试验,对其木梳制品进行实用和成本分析,最终确定50％左右的意杨压缩木完全能替代木梳传统原材料。     

杉木人工林地力衰退研究概述

介绍了我国杉木人工林地力衰退的现状，从森林土壤条件、杉木人工林的营养循环特点和人为经营措施等方面分析了导致杉木人工林地力衰退的原因，提出营建人工复层林、林地施肥、适当延长轮伐期，加强植被管理和进行定位观测等措施防止地力衰退。     

成年金丝小枣树移植及管理技术

为提高经济效益,减少损失,对金丝小枣成年大树移植技术进行了研究。论述了移植前的各项准备工作,移栽技术及移栽后的各项管理措施。     

柿炭疽菌的室内药效试验

通过在凹玻片上测试10种不同杀菌剂对柿炭疽菌分生孢子萌发、芽管伸长、附着孢形成的影响，对诱发凹玻片上菌丝孢子产生和在PDA上菌丝生长抑制作用的研究，认为不同的杀菌剂对不同菌态的炭疽菌药效不同。药剂筛选结果表明百菌清、退菌特、多菌灵3种杀菌剂药效最好，但它们对病原菌的作用机制不同。     

秃杉不同苗龄造林效果探讨

笛杉是我国特有的珍稀树种，但由于其１年生苗较小，各夺在引种中一般都采用２年生苗造林，效果不十分理论，经试验采用苗高１５cm以上，地径０．２５cm以上的１年生苗造林，只要造林时认真、细致，第６年后就与２年生苗造林生长效果相仿。１年生苗造林具有成活率高、育苗和造林成本低的特点值得在生产中推广。     

油茶织蛾生物学特性研究

Casmara patrona is one of the main borer pests damaging Camellia oleifera in China. Few reports about this insect were published. The life history, biological characteristics and natural enemies of C. patrona were investigated through field survey and laboratory observation in this study. The results showed that C. patrona had one generation in one year or two years, and overwintered as 3rd-5th instar larva in Zhejiang Province. About 88 percent of larvae began to pupate in early May, and the rest kept feeding till the next year. The adults could be observed from early June to early July, and the peak of emergence occurred in late June. The adults began to oviposit in late June and terminated in early July. The eggs started hatching in early July, and then the larvae bored into the branch to feed. The entrance holes of the larvae distributed predominately in the middle of the eastern and southern part of C. oleifera tree. The larva had five instars, and there were 10-50 days when the larva did not feed between consecutive instars.     

RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells

Inhibins are members of the TGFβ superfamily and act as suppressors of follicle stimulating hormone (FSH) secretion from pituitary glands via a negative feedback mechanism to regulate folliculogenesis. In this study, the INHBB gene was knocked down by three RNAi-Ready pSIREN-RetroQ-ZsGreen vector- mediated recombinant plasmids to explore the effects of INHBB silencing on granulosa cell (GC) cell cycle, apoptosis and steroid production in vitro. Quantitative real-time polymerase chain reaction, Western blot, flow cytometry and ELISA were performed to evaluate the role of INHBB in the mouse GC cell cycle, apoptosis and steroid production in vitro. The results showed that the relative mRNA and protein expression of INHBB in mouse GCs can be significantly reduced by RNAi with pshRNA-B1, pshRNA-B2 and pshRNA-B3 plasmids, with pshRNA-B3 having the best knockdown efficiency. Downregulation of the expression of INHBB significantly arrests cells in the G1 phase of the cell cycle and increases the apoptosis rate in GCs. This was further confirmed by downregulation of the protein expressions of Cyclin D1, Cyclin E and Bcl2, while the protein expression of Bax was upregulated. In addition, specific downregulation of INHBB markedly decreased the concentration of estradiol and progesterone, which was further validated by the decrease in the mRNA levels of CYP19A1and CYP11A1. These findings suggest that inhibin βB is important in the regulation of apoptosis and cell cycle progression in granulosa cells. Furthermore, the inhibin βB subunit has a role in the regulation of steroid hormone biosynthesis. Evidence is accumulating to support the concept that inhibin βB is physiologically essential for early folliculogenesis in the mouse.     

The Expression of Long Noncoding RNA (H19) in Mouse Skeletal Muscle Development

This experiment was intend to study the changes of long non-coding RNA (H19) expression levels in skeletal muscle development and regeneration,and lay the foundation of its mechanism reach in skeletal muscle development.C2C12 cell line and ICR mice were used as experimental material,bioinformatics assay was used to exploit its non-coding character and low conservatism in different species,and the expressions of H19 in C2C12 cell differentiation,skeletal muscle development and the phase of muscle regeneration were detected by quantitative Real-time PCR (qRT-PCR).The results showed that,H19 expression levels in postnatal mouse skeletal muscle decreased with increasing age;during C2C12 cell differentiation,H19 mRNA increased gradually,and then maintained a high level;The expression of H19 was maintained at a high level through days 4 to 6 after injury.In consideration of its express character in C2C12 cell differentiation and skeletal muscle damage repair model,H19 may play an important role in promoting myogenesis and skeletal muscle regeneration.