日光温室彩椒双干循环整枝栽培技术

采用双干循环整枝技术充分发挥了彩椒潜伏芽结果的能力,在其他管理措施相同的条件下每667 m2产量达12 000 kg以上,比常规双干整枝增产30%左右。     

Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System

To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention.     

柞蚕新品种永青的选育

柞蚕新品种永青是以“青六号”为基础材料,利用系统选育手段,采用分区饲养、系统分离、定向培育的方法,同时兼顾各项经济指标的综合平衡,通过9年18代的系统选育,育成的具有青黄蚕血统的柞蚕新品种。选育结果：全茧量12.47g,千粒茧重11.99kg,虫蛹统一生命率94.81%;与吉柞88-2、方山黄杂交,增产幅度达15.5%以上。     

基于改进入侵杂草算法优化的PID生物质废弃物发酵热能控制模式

生物质废弃物发酵系统是一个大时滞系统，针对生物质发酵系统中的热能控制存在波动大、振荡多和超调大的问题，提出基于改进入侵杂草算法优化的PID生物质废弃物发酵热能控制方法，运用改进入侵杂草算法对PID控制器的参数进行在线调整。算例仿真表明，所提方法能够快速、稳定且高精度地实现对生物质废弃物发酵系统的热能控制，为沼气制取提供了稳定的热能环境，实现了沼气的稳定供应。      

Effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and antioxidant capacity

This study investigated the effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and renal antioxidant capacity. Eighteen ewes pregnant were randomly divided into control group (CG, ad libitum, 0.67 MJ ME·BW^−0.75·day⁻¹, n = 6), restricted group 1 (RG1, 0.18 MJ ME·BW^−0.75·day⁻¹, n = 6), and restricted group 2 (RG2, 0.33 MJ ME·BW^−0.75·day⁻¹, n = 6). At 140 days, the fetal blood, allantoic fluid and kidney tissue were collected to determinate fetal renal function and renal antioxidant capacity. The results showed that the fetal weight, kidney weight, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), aquaporin-2 (AQP-2) and aquaporin-3 (AQP-3), and total antioxidant capacity (T-AOC) in RG1 group were decreased compared with the CG (P < 0.05), but the contents of β2-Microglobulin (β 2-MG), cystatin C (Cys-C), filtered sodium excretion fraction (FENa), malondialdehyde (MDA), and hydroxyl radical (OH) in RG1 group were increased (P < 0.05). The impaired ovine fetal renal growth, antioxidant imbalance and dysfunction of glomerulus ultrafiltration, and the renal tubules reabsorption were induced by maternal malnutrition during late pregnancy.     

制度有效性检验:基于实验经济学的一个综述

长期以来，制度有效性的检验问题一直制约着制度经济学的发展，而近期实验经济学中大量的机制设计实验为其提供了一种新的检验手段。通过实验经济学中公共物品自愿捐赠机制对有关正式制度与非正式制度的比较研究，同时梳理相应文献，其文献大致可分为两类：外生制度静态比较与内生制度研究。外生制度的静态比较是指在可控制条件下检验不同制度的有效性，着眼于比较不同制度下的贡献度以及社会福利等问题；而内生制度则扩展了有效性检验，人们可以通过选择不同的制度或治理结构来约束其行为，进而检验制度的稳定性。实验方法弥补了主流经验研究中不能对制度进行有效性检验这一不足，为研究制度经济学提供了一条新的思路。     

笃斯越橘组织培养增殖生长结果分析

文章根据培养基类型、细胞分裂素浓度及生长素浓度的不同采用L9(3)4正交设计对笃丝越橘组织培养的增殖生长进行试验。     

农机与农艺结合中存在的问题及对策

农机与农艺紧密结合,对于农业经济发展具有重要意义,是未来农业发展的必然趋势。阐述农机与农艺结合的重要性,分析农机与农艺结合过程中存在的问题,提出加快农机与农艺结合的有效措施,以期为现代农业的发展提供参考。     

小麦族的基因组显性及其育种学意义

小麦族包含大量由不同基因组组成的异源多倍体物种。同一个异源多倍体物种的不同基因组可能对表型性状产生非对称性贡献,例如小麦属多倍体物种的形态分类特性,更像其A基因组供体物种,这种现象称为A基因组显性。由于基因组显性,小麦族形成了以A、D、U、St为轴心(显性)基因组的异源多倍体物种簇。异源多倍体物种的基因组显性可能与其进化适应优势的形成有关。在育种方面,基因组显性影响多倍体新作物开发及小麦-外源染色体易位设计。     

凤庆茶叶种植与混林茶园生态系统研究

中国云南是世界茶树的原产地,而云南省凤庆县又是滇红的诞生地,具有悠久的茶树栽培种植历史,其混林茶园生态系统也具有较高的研究价值。本文通过对其茶叶种植史和混林茶园生态系统进行梳理和研究,厘清凤庆县茶叶种植的历史发展脉络,挖掘凤庆混林茶园生态系统的优势及其应用价值。