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51.
Sato José Paulo Hiroji Gava Danielle Schaefer Rejane Cantão Maurício Egídio Ciacci-Zanella Janice Reis de Barcellos David Emilio Santos Neves 《Tropical animal health and production》2017,49(5):1071-1075
Tropical Animal Health and Production - Porcine circovirus type 2 (PCV2) has been identified in pig population in Brazil since 2000, but scarce studies involving wild boars with PCV2 infection are... 相似文献
52.
Isidre Llorente Albert Vilardell Pere Vilardell Elisabetta Pattori Riccardo Bugiani Vittorio Rossi Emilio Montesinos 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(1):127-141
Stemphylium vesicarium, the causal agent of brown spot of pear, overwinters in the leaf residues of pear and herbaceous plants of the orchard floor.
Pseudothecia of the teleomorph, Pleospora allii, are formed on these residues where they produce ascospores. New methods were tested aimed at reducing this overwintering
inoculum and increasing the efficacy of control of brown spot of pear. Sanitation methods were evaluated in nine trials in
Girona (Spain) and Ferrara (Italy) over a 4-year period. The sanitation methods were leaf litter removal in December to February,
and application of biological control agents (commercial formulates of Trichoderma spp.) to the orchard ground cover from February to May. Fungicides were also applied to the trees during the pear-growing
season, scheduled according to the BSPcast model. The different methods were tested as stand-alone applications or in combination.
All methods consistently reduced the disease incidence at harvest on fruit with an efficacy between 30 to 60% for leaf litter
removal and more than 60% for the combination of leaf litter removal and biological control. Efficacy of sanitation alone
(leaf litter removal and biological control) in reducing the brown spot level on fruit was similar in most of the trials to
the efficacy obtained when fungicides were applied alone. However, integration of sanitation methods and fungicides did not
improve the efficacy of disease control over the level provided by fungicides alone. 相似文献
53.
54.
Andrew Park Michiel van Breugel Mark S. Ashton Mark Wishnie Emilio Mariscal José Deago Diogenes Ibarra Norma Cedeño Jefferson S. Hall 《Forest Ecology and Management》2010
Native Neotropical trees are being increasingly planted for restoration purposes and timber production, but we lack species-specific data on growth responses to different regional climates and local environmental variation. We used regression trees and variance components to quantify the effects of within- and among-site environmental variation on the basal area (BA) of 21 Neotropical and two exotic tree species at three selection trials in the Republic of Panama. Sites represented distinct regional climates in which annual rainfall varied from 1100 to 2226 mm, with dry seasons of 4.1–6.7 months. Local environmental variables included measures of slope steepness and position, soil texture, soil color, and indicators of soil condition, such as subsoil rockiness. 相似文献
55.
56.
Silva V Genta G Möller MN Masner M Thomson L Romero N Radi R Fernandes DC Laurindo FR Heinzen H Fierro W Denicola A 《Journal of agricultural and food chemistry》2011,59(12):6430-6437
The antioxidant capacity of propolis from the southern region of Uruguay was evaluated using in vitro as well as cellular assays. Free radical scavenging capacity was assessed by ORAC, obtaining values significantly higher than those of other natural products (8000 μmol Trolox equiv/g propolis). ORAC values correlated well with total polyphenol content (determined by Folin-Ciocalteu method) and UV absorption. Total polyphenol content (150 mg gallic acid equiv/g propolis) and flavonoids (45 mg quercetin equiv/g propolis) were similar to values reported for southern Brazilian (group 3) and Argentinean propolis. Flavonoid composition determined by RP-HPLC indicates a strong poplar-tree origin. Samples high in polyphenols efficiently inhibit low-density lipoprotein lipoperoxidation and tyrosine nitration. In addition, Uruguayan propolis was found to induce the expression of endothelial nitric oxide synthase and inhibit endothelial NADPH oxidase, suggesting a potential cardiovascular benefit by increasing nitric oxide bioavailability in the endothelium. 相似文献
57.
In order to evaluate the protection conferred by an experimental inactivated vaccine against infectious coryza, three challenge trials were undertaken using 112 1-day-old broilers. The vaccine "Hepa Inmuno NC" included bacterial antigens of Avibacterium paragallinarum (serogroups A, B, variant B, and C) as well as antigens of Newcastle virus and hepatitis virus. Fifty-six broiler chicks were vaccinated at the first day of life at the hatchery while another 56 chicks were left unvaccinated. Three infection trials were conducted simultaneously using each of the three serogroups A, B, or C of Av. paragallinarum. In each trial, 17 vaccinated and 17 unvaccinated broilers were used. Challenge was performed at day 31 of life by injection, into the left infraorbital sinus, of approximately 1 x 10(5) colony forming units of the corresponding Av. paragallinarum strain. Clinical signs were recorded on day 2 postchallenge. All broilers were euthanatized and both infraorbital sinuses were bacteriologically examined for the presence of Av. paragallinarum on day 5 postchallenge. In comparison with the unvaccinated broilers, the vaccine significantly reduced the number of broilers with clinical signs after challenge with serogroup B, and significantly fewer vaccinated broilers were positive for the presence of Av. paragallinarum after challenge with serogroup C. On the other hand, no significant protection was observed when broilers were challenged with Av. paragallinarum from serogroup A. Despite the high infection rates in vaccinated chicks after an experimental infection with Av. paragallinarum, it was possible to reduce colonization of Av. paragallinarum (serogroup B) and clinical signs (serogroup C) in broiler chicks by vaccination at the first day of life. Further cross-protection trials should be done, including other Av. paragallinarum strains in the vaccine, especially those from serogroup A. 相似文献
58.
Dongmo Albert Nanfack Nguefack Julienne Dongmo Joseph Blaise Lekagne Fouelefack François Romain Azah Rene Udom Nkengfack Ephrem Augustin Stefani Emilio 《植物病害和植物保护杂志》2021,128(3):703-713
Journal of Plant Diseases and Protection - The high cost of chemical pesticides and their negative impact on the environment prompted the search for natural pesticides from plants. The objective of... 相似文献
59.
Aparicio-Fernández X Reynoso-Camacho R Castaño-Tostado E García-Gasca T González de Mejía E Guzmán-Maldonado SH Elizondo G Yousef GG Lila MA Loarca-Pina G 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(1):35-40
Jamapa bean is a black Phaseolus vulgaris variety rich in condensed tannins, anthocyanins and flavonols with interesting biological activities. The objective of this
work was to evaluate the antiradical capacity (ARC) of a Jamapa bean methanolic extract (BME) and some of the proanthocyanidin-rich
fractions derived from it, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The effect of the BME on some proteins involved
in apoptosis on HeLa cells was also evaluated. A strong correlation between proanthocyanidin concentration in BME and antiradical
capacity was found, suggesting that these compounds contribute significantly to antiradical activity. BME was a better radical
scavenger than butylated hydroxytoluene (45.6 and 33.9% ARC at 400 μM, respectively). Two proanthocyanidin-rich fractions
obtained after a preliminary separation of the BME using Toyopearl (TP4 and TP6) exhibited a higher antiradical activity than
the parent extract. The treatment of HeLa cells with 35 μg BME/ml/24 h increased the expression of Bax and Caspase-3, pro-apoptotic
proteins (6.13 and 1.2 times for Caspase-3 and Bax, respectively). The mechanism of action of some proteins involved in apoptosis
was also evaluated, and the results suggest that black Jamapa bean could be an important source of polyphenolic compounds
with potential biological use as antioxidant and anticancer agents. 相似文献
60.
Llop P Donat V Rodríguez M Cabrefiga J Ruz L Palomo JL Montesinos E López MM 《Phytopathology》2006,96(8):900-907
ABSTRACT An atypical strain of Erwinia amylovora was isolated near an outbreak of fire blight at a nursery in Spain in 1996. It was obtained from a Crataegus plant showing typical symptoms and was identified as E. amy-lovora by biochemical tests and enrichment-enzyme-linked immuno-sorbent assay, but not by polymerase chain reaction using primers based on the pEA29 sequence. Nevertheless, with primers from chromosomal regions, the isolate gave the expected amplification band. This strain carries one plasmid of approximately 70 kb, with no homology with the 29-kb plasmid common to all pathogenic strains, or with a large plasmid present in some E. amylovora strains. Growth of the strain in minimal medium without thiamine was slower compared with cultures in the same medium with thiamine, a characteristic typical of strains cured of the 29-kb plasmid. Nevertheless, aggressiveness assays on pear, apple, and Pyracantha plants and in immature pear fruit showed that this strain exhibited a virulence level similar to other strains containing pEA29. To the best of our knowledge, this is the first report of the isolation from naturally infected plant material of a pathogenic strain of E. amylovora without pEA29, but with a plasmid of approximately 70 kb not previously described. 相似文献