The juvenile growth and carcass traits were evaluated in a 4 × 4 full diallel crossing involving four colored broiler chicken
lines viz., Naked neck (NN), Dwarf, Punjab Broiler-1 (PB-1), and Punjab Broiler-2 to study the performance and crossbreeding
parameters. The data on 2,280 chicks were analyzed using least squares techniques to assess the effect of genetic group, and
the significant traits were further analyzed for crossbreeding parameters. Genetic group had significant influence on the
body weights and carcass traits. The cross of PB-1 × NN recorded significantly (p ≤ 0.05) higher body weight at 6 weeks of age. The data revealed that general combining ability (GCA), specific combining
ability (SCA), maternal ability (MA), sex-linked effect (SE), and purebred effects significantly influenced the growth and
carcass traits in the broiler crosses. The crossbred PB-1 × NN showed positive heterosis for growth as well as carcass traits.
The results suggest that GCA, SCA, SE, and MA are important in the inheritance of the body weights and carcass traits indicating
the presence of both additive and nonadditive genetic variation along with reciprocal effects. Therefore, pure line selection
along with development of specialized sire and dam lines followed by crossing will improve the growth performance and benefit
the poultry farmers. It is concluded that PB-1 as a male line and NN as a female line performed significantly higher, therefore,
may be used for improving the performance of colored broilers. 相似文献
The potential of municipal solid waste compost (MSWC) was evaluated for rice cultivar, IET- 1444, during the wet seasons of 1997-99. Field experiments were conducted at the Agriculture Experimental Farm of Calcutta University, West Bengal, India using MSWC and cow dung manure (CDM), with or without urea (U), and fertilizers (F). Dry matter accumulation was highest with CDM + U, followed, in a descending order, by MSWC + U and F. Panicle length was largest and thousand–grain-weight were highest for F. Application of CDM alone gave 30% higher grain yield over MSWC but the latter, along with urea, increased grain yield by 49% over MSWC alone. Uptake of N by straw and grain was significantly higher with CDM as compared to MSWC. Percent N recovery was higher when fertilizer in the form of urea was applied with either CDM or MSWC. Uptake of heavy metals (Zn, Cu, and Pb) by straw and grain of rice was lower with the application of MSWC alone than with the other treatments except control. Uptake of Cd was below detection limit. There was no significant variation between the physico-chemical properties of soil due to the application of these treatments. Results indicated that MSWC application to rice was effective and safe to be used as an alternative organic supplement. 相似文献
Testosterone, 3,17-dihydroxy-5-pregnen-20-one, 17,20-dihydroxy-4-pregnen-3-one (17,20P) and 5-pregnane-3,17,20-triol were identified as the major metabolites of [3H] 17-hydroxyprogesterone in ovarian incubations of the European catfish Silurus glanis. 17,20P and the reduced triol were present only in ovaries from fish primed with carp hypophysial homogenate (chh) while testosterone yields were significantly higher in controls than in treated fish. 11-Ketotestosterone, 11-hydroxytestosterone and 17,20-dihydroxy-4-pregnen-3-one (17,20P) were identified as the major metabolites of [3H]17-hydroxyprogesterone in in vitro incubations of testes of a spermiating catfish. There was no significant production of conjugates or other water soluble metabolites by either sex. The stimulation of plasma 17,20P, 17,20P and 11-hydroxytestosterone by chh in primed but not control males suggests that the role of these steroids in spermiation should be further examined. 相似文献
Oocyte growth in most oviparous vertebrates including fish is due to the formation of yolk, and eggshell proteins (zona radiata
proteins). Zonagenesis leads to the formation of zona radiata proteins in oocytes, which play an important role during oogenesis,
whereas vitellogenesis leads to the formation of yolk in oocytes through a series of events during which the yolk precursor
protein vitellogenin (Vg) is synthesized and secreted from liver into blood from where it is sequestered into the developing
oocytes and thereafter proteolytically cleaved to form yolk proteins (YPs) and finally deposited in the ooplasm. Much research
has been done in many fish species with respect to the number and nature of Vg and YPs and their probable functions during
fish reproduction. Recent findings of multiplicity of Vg molecules in fishes reject the earlier view of a single-Vg model
and have led scientists to explore the functions of individual Vg and their YP derivatives, lipovitellin, phosvitin, and β′-component.
Two distinct types of Vg or Vg genes, containing or encoding the three YPs, have been detected in many teleosts. A third unusual,
incomplete, phosvitin-poor Vg has been described recently in many fishes. In comparison to much of the information on vitellogenesis
in many fishes very little is known for Indian fishes. In India research has been done in a few species such as the catfish,
Heteropneustes fossilis and Clarias batrachus, the murrel, Channa punctatus and the Indian major carps, Labeo rohita and Cirrhinus mrigala. Immunological and biochemical analyses suggest the occurrence of multiple forms of Vg and their YP derivatives. The synthesis
and incorporation of Vg are regulated by gonadotropin (GTH) and estradiol-17β (E2). A differential role between estrone (E1) and estriol (E3) has been demonstrated for Vg synthesis. Enzyme-linked immunosorbent assays (ELISAs) for Vg have been developed to measure
plasma Vg. Finally the different roles of Vg1 (HAI) and Vg2 (HAII) on vitellogenesis have been demonstrated. However, more
research remains to be carried out in other fish species with respect to the number and nature of Vg and YPs and their genes
in order to describe their reproductive functions. 相似文献
The environment is currently polluted by thousands of chemicals or xenobiotics introduced into the environment by man to meet
the demands of the modern era. Every day we encounter this negative side of human civilization, but have done little to lessen
the rate of pollution. Although the entire biosphere is polluted it is water resources that are the most polluted because
water is the ultimate sink for many contaminants. Thus, fish are the most vulnerable of all animal species. They are helpless
because they cannot avoid the polluted habitat and face this contamination by default. Nevertheless, fish are found to survive
under extreme conditions when their natural habitat has been compromised to a great extent. However, fish are highly sensitive
to small environmental changes and their populations gradually dwindle if pollution continues unabated. However, we know that
there are instances when water is cleaned and the rate of repopulation by different fish species has gained momentum, restoring
the ecological balance. Thus, fish are considered reliable bioindicators of water pollution and fish ecotoxicology has received
much attention in recent years, and fish toxicology has been able to defend a significant position in the arena of xenobiotics
research over the years. This review deals with some of the major intoxication and detoxication signals manifested by fish
exposed to arsenic (As), which is presently one of the most worrying metalloids in water pollution. 相似文献
We have examined the genes of the endothelin system that are targets for regulation by prostaglandin F2alpha (PGF2alpha). The effects of a luteolytic dose of PGF2alpha ) on the mRNA encoding endothelin converting enzyme-1 (ECE-1), pre-pro endothelin-1 (pp ET-1) and the ET receptors ETA, ETB, in bovine corpus luteum (CL) during the early (days 1 and 4), mid (day 10) or late (day 17) luteal phases were examined. The effect of the PGF(2alpha) treatment on ECE-1 protein, Big ET-1 and the biologically active mature ET-1 peptide were also examined. Most importantly, the direct ECE-1 activity was determined. Before day 10 of the cycle, in a PGF2alpha-independent manner, the amounts of mRNA encoding ET-1, ECE-1, ETA, and ETB were increased steadily from day 1. After day 10 of the cycle, expression of mRNA encoding pp ET-1 and ETA acquired responsiveness to exogenous PGF2alpha and both genes were up-regulated by the PGF2alpha treatment. This effect of PGF2alpha was also detected for the proteins corresponding to the mature ET-1. The enzymatic activity of ECE-1 remained unchanged throughout the lifespan of the CL in spite of the detected changes in mRNA and protein. The results suggest that the luteal endothelin system is regulated in a PGF2alpha-independent and -dependent manner. Importantly, an alteration in luteal ET-1 availability is most likely achieved by modulating the expression of mRNA encoding pp ET-1 and not by the amount or activity of ECE-1. This interpretation is supported by the observation that the activity of ECE-1 remained unchanged throughout the ovarian cycle. The combined effects of greater ET-1 availability and gene expression encoding the ETA receptor in the late luteal phase could render the CL, at this developmental stage, more sensitive or responsive to ET-1. If the luteal tissue is responsive to the available ET-1 during the early phase of the ovarian cycle, an additional role for ET-1 should be considered beyond mediating the luteolytic actions of PGF2alpha. Agents blocking the actions of ET-1 might be the best approach to interfere with the luteal ET system and test its physiological role(s) in vivo. 相似文献
1. Two candidate genes, namely, Gonadotropin releasing hormone I (GnRHI) and Gonadotropin releasing hormone II (GnRHII) play pivotal roles in ovulation and egg production in chicken. The objective of this study was to explore polymorphism in these genes and to estimate the effects of polymorphism of these two genes on egg production and egg quality traits in White Leghorn laying hens.
2. Single strand conformation polymorphism followed by sequencing was performed to detect polymorphism in these genes.
3. The coding regions of the GnRHI and GnRHII genes were found to be polymorphic. In the GnRH1 gene, 12 haplotypes were determined, of which the h1 haplotype was predominant and the h5, h9 and h11 haplotypes were the least frequent ones. In the GnRHII gene, eight haplotypes were found, of which the h1 haplotype was the most frequent and the h6 was the least frequent haplotype in the White Leghorn population.
4. The haplogroups of GnRHI had a significant effect on body weight and egg production up to 64 weeks of age, yolk content, Haugh units and egg shell parameters. The h1h2 haplogroup of the GnRHI gene showed the highest egg production, with 211.0 ± 24.3 eggs up to 64 weeks of age, while the highest yolk content and Haugh unit was found in h3h10 haplogrouped birds. The haplogroups of GnRHII had a significant effect on age at sexual maturity (ASM) where the shortest ASM was found in the h1h4 birds (147.3 ± 5.9 d) and the longest ASM was observed in the h1h3 birds (160.6 ± 23.4 d).
5. It was concluded that GnRHI and GnRHII genes are polymorphic and have a significant effect on body weight, egg production and egg quality traits in White Leghorn laying hens. 相似文献