Management strategies for sustainable yield of potato crop under high temperature

High temperature has a deleterious effect in productivity of cool season crops like potato. This study was conducted to assess the efficacy of soil management practices to high-temperature tolerance in potato. Two popularly grown potato cultivars of northeast India, Kufri megha and Rangpuria, were sown under optimal and high-temperature conditions with soil application of inorganic nutrients (N, P, K, Ca, Zn), organic amendment, i.e. farm yard manure (FYM), and straw mulch. This integrative soil management practice showed significant positive influence on membrane integrity, chlorophyll content, total soluble sugars and superoxide dismutase activity of potato plant. Higher tuber bulking rate and tuber yield in both optimal and high-temperature situation was recorded under these soil applications. Kufri megha performed better with the application of Ca and Zn along with straw mulch, while Ca with farm yard manure substantially improved restore yield in Rangpuria. Application of CaSO₄ and ZnSO₄ (20 and 60 kg ha^?1, respectively) with FYM and straw mulch (10 and 6 t ha^?1, respectively) can mitigate the high-temperature stress in potato grown in acidic soil (deficient of Ca and Zn) of northeast India.     

Time-dependent changes in dough color in hexaploid wheat landraces differing in polyphenol oxidase activity.

Time-dependent changes in the color of noodle sheets (using 2% NaCl or 1% alkaline salts in the formulation) made from 43 Iranian hexaploid wheat landrace accessions were measured. Pekar slick tests in water and in alkaline conditions were also carried out. A wide variation in color characteristics of the landraces was found, with L values of salted noodle sheets at 2 h ranging from 80.9 to 89.2 and b values of alkaline noodle sheets at 2 h ranging from 19.1 to 27.4, showing potential application in noodle wheat improvement programs. For initial rapid screening of samples it was observed that a single reading of the dough sheet after 2 h was adequate. The dough sheets should be kept at 5 degrees C during storage, to prevent microbiological activity in the dough, which would give erroneous results. The Pekar slick test results were not highly correlated to color measurements on the dough, so this test is not recommended for screening for noodle color potential in landraces.     

Somatic embryogenesis of Limonium sinense: a study on the regeneration efficacy via direct and indirect approach followed by Agrobacterium-mediated genetic transformation

An efficient indirect somatic embryogenesis and Agrobacterium-mediated transformation protocol for Limonium sinense has been established, wherein neomycin phosphotransferase II (npt II) and β-glucuronidase (GUS) genes were used as selectable and screenable markers, respectively. The efficiency of plantlet regeneration from transformed tissue was compared between direct embryogenesis from leaf and indirect embryogenesis from callus. Embryogenic callus (EC) was initiated from leaf explants on MS medium supplemented with 6.7 μM 2,4-D and 2.22 μM BA. The somatic embryos were induced, matured, and germinated when ECs were transferred onto MS medium supplemented with 4.44 μM BA and 1.07 μM NAA. Agrobacterium tumefaciens strain LBA 4404 containing the vector pBI121 was used for the transformation. Transient GUS expression frequency was evaluated and putative transgenic plants were successfully grown on culture medium in presence of kanamycin (80–100 mg L^?1). PCR analysis of putative transgenic plants confirmed the presence of GUS and nptII genes. The transformation efficiency obtained through indirect embryogenesis from calluses (4%) was much higher than through direct embryogenesis from leaf explants (0.9%).