Risk factors for Thoroughbred racehorse fatality in jump starts in Victoria, Australia (1989-2004)

REASONS FOR PERFORMING STUDY: The risk of fatality is greater in jump than in flat racing in Victoria, Australia. This is the first study to identify risk factors specific to jump starts in Victoria. OBJECTIVE: To identify risk factors for fatality of Thoroughbred racehorses in jump starts on all racecourses in Victoria, Australia between 1989 and 2004. METHODS: Fatalities comprised all horses that died during or immediately after a jump (hurdle or steeplechase) race or official jump trial and all horses that were subjected to euthanasia within 24 h of an event in which an injury was sustained. The retrospective study involved 191 case starts and 2324 control starts. Univariable and multivariable backward stepwise logistic regression was used to identify risk factors for fatality at any one start. A multiple level model was used with racecourse included as a random effect. RESULTS: In the final multivariable model, the duration of the racing career of the horse, the number of flat, hurdle and steeple starts accumulated in the 60 days prior to the case or control start, the number of flat and jump starts accumulated over the racing career, if the horse had had a start between 1 and 14 days prior to the case or control start, the type of jump race (hurdle or steeple), the calendar year of the start and the location of the racecourse were associated with fatality. CONCLUSIONS: The findings highlight the need to investigate further the differences between hurdle and steeplechase events and the adverse effect of prolonged prior flat racing careers on the risk of fatality in jump starts. POTENTIAL RELEVANCE: This is the first study to examine risk factors for fatality in jump starts in Victoria. The results should shape the development of interventions to reduce the risk in jump starts in the future.     

Myxoma virus in the European rabbit: interactions between the virus and its susceptible host

Myxoma virus (MV) is a poxvirus that evolved in Sylvilagus lagomorphs, and is the causative agent of myxomatosis in European rabbits (Oryctolagus cuniculus). This virus is not a natural pathogen of O. cuniculus, yet is able to subvert the host rabbit immune system defenses and cause a highly lethal systemic infection. The interaction of MV proteins and the rabbit immune system has been an ideal model to help elucidate host/poxvirus interactions, and has led to a greater understanding of how other poxvirus pathogens are able to cause disease in their respective hosts. This review will examine how MV causes myxomatosis, by examining a selection of the identified immunomodulatory proteins that this virus expresses to subvert the immune and inflammatory pathways of infected rabbit hosts.     

Optimising cutting method and timing for the control of Abutilon theophrasti seed production

Abutilon theophrasti (velvetleaf) is a widespread and problematic annual weed. Greenhouse and field experiments were conducted to investigate the effects of different cutting methods on the viability of A. theophrasti seeds. Three cutting methods were assessed: (1) Entire plant cut and dried (EPD)—plants were cut at soil level and dried with capsules attached on the greenhouse bench or soil surface for 4 weeks; (2) capsules detached and dried (CD)—capsules were removed from plants and dried for 4 weeks; and (3) capsules detached and tested while fresh (CF)—a control treatment. Before drying, the developmental stage (stage one, dark green; stage two, light green; stage three, yellowish-green; or stage four, black with the slightly open capsule) and age (days after flowering, DAF) of each capsule was recorded. Seed viability was measured immediately in the CF treatment and after the 4-week drying period in the EPD and CD treatments. No seeds in the EPD and CD treatments were viable when harvested at the first developmental stage (1–8 DAF) in either experiment, but 100% of seeds in the CF treatment in the field were viable when harvested at 8 DAF. In both greenhouse and field experiments, seeds attained full viability at earlier harvest ages in CF than in EPD or CD treatments, suggesting that seeds might become viable relatively early in development but lose viability if allowed to dry. These findings could be applied to optimise late-season mechanical control of A. theophrasti.     

Water Pricing and Sustainability of Self-Governing Irrigation Schemes

The sustainability of self-governingirrigation schemes is currently underpressure in many countries as publicfinancial support is decreasing.Furthermore, growing global concernregarding water scarcity means thatefficient water use is essential.Theoretically the choice and implementationof a water-pricing system should play acentral role in achieving this objective,both by recovering water costs and byencouraging farmers to adopt more efficientbehaviour. In the Senegal River Delta newlyestablished water users associations (WUAs)have chosen low water charges, which areaffordable for the majority of farmers butwhich underestimate long-term maintenancecosts. Combined with the difficulty ofcollecting and managing farmers' fees, thischoice has drawn them into a vicious circleleading to scheme deterioration and poorwater service. New alternatives have beendiscussed with them using a simulation toolthat takes into account both the watercosts and the farmers' incomes. Comparedwith the present fixed water charge basedonly on cultivated land, a two-part optionbased both on equipped and irrigated areasensures the recovery of fixed expenses suchas maintenance, while encouraging farmersto intensify their farming systems byadopting double cropping. Neverthelesschoosing a relevant water charge does notensure by itself the sustainability of anirrigation scheme. Combined supportproviding WUAS and farmers with efficientadvice regarding management andorganisational skills should alsocontribute significantly to achieve thisprime objective.     

Effects of Co‐stocking Smallmouth Buffalo,Ictiobus bubalus,with Channel Catfish,Ictalurus punctatus

Proliferative gill disease (PGD) in catfish is caused by the myxozoan Henneguya ictaluri. The complex life cycle requires Dero digitata as the oligochaete host. Efforts to control PGD by eradicating D. digitata have been unsuccessful. Smallmouth buffalo, Ictiobus bubalus, (SMB) are opportunistic bottom feeders and a putative option for controlling D. digitata. In 2011, 15 ponds (0.4 ha) were stocked with 5000 channel catfish, Ictalurus punctatus; 7 of these 15 ponds were also stocked with 300 SMB fingerlings. There were no differences in benthic invertebrate numbers or water quality variables between ponds with or without SMB. At harvest, there were no differences in percent survival, total weight, or catfish feed conversion ratio. In the second year, 18 ponds (0.4 ha) were stocked with 6000 channel catfish. Half the ponds were also stocked with 300 SMB. Sentinel fish were used to estimate disease severity, and pond water was collected for molecular estimation of H. ictaluri actinospore concentrations. Similar to the first year, there were no differences between treatments in any variable tested, including PGD severity in sentinel fish and parasite concentrations in pond water. Under these study conditions, presence of SMB did not have a measureable effect on PGD incidence, parasite density, or overall catfish production.     

Identification of genetic regions associated with black point in barley

Black point (BP) can cause severe losses to the barley industry through downgrading and discounting of malting barley. The genetic improvement in BP resistance of barley is complex, requiring reliable screening tools, an understanding of genotype by environment interactions and an understanding of the biochemical mechanisms of melanisation involved in BP development. Thus the application of molecular markers for resistance to BP may be a useful tool for plant breeders. We have investigated the genetic regions associated with BP resistance in the barley F₂ population, Valier/Binalong. Quantitative trait loci (QTLs) contributed by the resistant parent Valier, were detected on chromosomes 2HS, 2HC, 3HL, 4HL and a QTL contributed by the susceptible parent, Binalong was detected on 5HL. Three of the four QTLs were detected in two distinctly different environments. The differences observed in BP resistance between these two environments and the implications for accelerated screening are discussed. Identified SSR markers in these regions may be useful for selecting black point resistance in related breeding materials.     

From shallow to deep waters: habitats used by larval lampreys (genus Petromyzon and Lampetra) over a western European basin

Abstract –  Habitat requirements of lamprey ammocoetes (Petromyzon marinus and Lampetra genus) were investigated, for the first time, from shallow to deep waters, at different spatial scales across the Gironde‐Dordogne continuum, thanks to a water suction dredge. Fish‐habitat relationships were assessed through two complementary statistical analyses: habitat‐use curves and habitat suitability models using the Boosted Regression Trees (BRT) technique. Analyses were performed on a small‐size data set that was characterised by the low prevalence of lamprey. The sea lamprey larvae occurred in deeper areas than their Lampetra genus counterparts. ‘Pools’ of 2 m’ depth and more were optimal habitats for the former species. Among the environmental variables retained to model lamprey occurrences, the mesohabitat (a categorical variable) was demonstrated to be highly influential, in terms of fine grain‐size substratum and vegetation cover. These preliminary results suggest that monitoring using the water suction dredge method may contribute to sea lamprey conservation.     

Application of reverse-phase h.p.l.c. for the determination of partition coefficients

Reverse-phase high performance liquid chromatography (h.p.l.c.), using a C₁₈ analytical column, has been applied to the determination of partition coefficients for a range of agrochemicals and industrial chemicals. Using a correlation plot of the logarithm of the capacity factor (k) with the logarithm of the n-octanol/water partition coefficient (P_ow), partition coefficients were predicted with a 95% tolerance interval of ± log 0.80 of the literature ‘shake flask’ value for compounds of random structure over the log P_ow range 0–6. Individual regression lines were fitted for compounds of comparable size and functional grouping, which reduced any bias and thereby enabled more accurate predictions to be made. The reverse-phase h.p.l.c. method has a number of advantages over the traditional ‘shake-flask’ method. Quantitative methods are not required or do not have to be developed and only the determination of the retention time is necessary. Quick and precise determinations of retention times are facilitated by h.p.l.c. and further improvement can be obtained by automation of solvent mixing, solute injection and data processing. H.p.l.c. was used to generate partition coefficient data for highly hydrophobic materials and, because of its resolving power, data for mixtures and solvent fractions. Dual detection, using u.v. and r.i. in series, was necessary for some compounds, particularly unknown mixtures and impure compounds. Calculations of log P_ow based on the fragment-addition method using the structural data file, MACCS, was of considerable value in confirming experimentally derived values. In certain cases, calculated log P_ow values were considered more trustworthy than experimental values.