Eosinophilic diseases in two Cavalier King Charles spaniels

This report describes the clinical presentation of two Cavalier King Charles spaniels with different eosinophilic diseases. The first case presented with dyspnoea and a non-productive cough, and investigations demonstrated eosinophilic bronchopneumonopathy. The second dog was referred for the investigation of haemorrhagic vomiting and diarrhoea and was eventually diagnosed with eosinophilic enteritis. Both dogs had concurrent eosinophilic stomatitis, and both responded completely to immunosuppressive glucocorticoid therapy. This report is the first to describe the concurrence of eosinophilic stomatitis and systemic eosinophilic disease in Cavalier King Charles spaniels, and suggest that this breed may be predisposed to eosinophilic syndromes.     

外来有害生物适生性研究方法及需要注意的若干问题

在分析利用生态位技术结合气候统计学方法及利用试验数据建立预测模型评估开展有害生物适生性研究的大量文献的基础上,本文详细讨论了在有害生物适生性研究中涉及的有关有害生物生物学资料的评估与分析、有害生物分布点的选择及预测结果的准确性等问题。认为有害生物的生物学特性及地理学的资料在研究有害生物适生性方面具有十分重要的作用。从统计学角度探讨了提高适生性预测结果正确性的途径。     

南方菜豆花叶病毒(SBMV)两典型株系特异cDNA和RNA探针的制备及应用

南方菜豆花叶病毒（ＳＢＭＶ）主要有２株系：菜豆株系（ＳＢＭＶ－Ｂ）和豇豆株系（ＳＢＭＶ－Ｃ）,血清学方法不能予以区分。根据已报道的核酸序列设计了２对特异引物,进行ＲＴ－ＰＣＲ扩增并克隆到载体Ｂｌｕｅｓｃｒｉｐｔ中,序列测定予以证实后利用随机引物法合成放射性ｃＤＮＡ探针,再进行体外转译合成地高辛ＵＴＰ标记的ＲＮＡ探针。２种探针均可分别特异地检测Ｎｏｒｔｈｅｒｎｂｌｏｔ膜上的ＳＢＭＶ－Ｂ和ＳＢＭＶ－Ｃ。ＲＮＡ探针检测ＳＢＭＶ－Ｂ和ＳＢＭＶ－Ｃ灵敏度分别为：０．１μｇ和０．０１μｇ。