卷烟感官评价三点检验Excel插件的设计和应用

为提高卷烟感官评价三点检验的易用性和实用性,采用Excel VBA设计三点检验Excel插件程序,随机生成样品编码,快速识别评价样品,自动统计分析结果,实现了卷烟感官的高效评价。     

荷花青熟种子播种繁育技术

受时空等环境条件限制,获取荷花青熟种质资源,对其繁育技术进行研究,为种质收集及开发应用提供基础性技术支持.以当年采集的热带型荷花青熟种子为材料,通过增温、增光、控水、防病等措施,创建适宜的生长环境,观测青熟种子发芽及小苗生长性状指标,结果表明:荷花青熟种子播种,充足水分且水温在25℃,可以实现出芽,种子的成熟度在6成以上时,出芽率有增加趋势.出芽后,立叶前期,控制水温在20℃以上,光照时间15h以上,可成苗;立叶期,温度控制在15℃以上,光照8h以上,可保苗;此期小苗生长缓慢.     

基于GM(1,1)模型的食用菌价格预测

基于灰色系统理论,根据香菇、平菇、双孢蘑菇、金针菇和黑木耳五大食用菌品种的月度批发价格数据,建立GM(1,1)预测模型,并通过相对误差、关联度、后验差检验和小误差概率对模型的合理性和精度进行检验,最后应用模型预测了2017年全国食用菌价格。研究结果表明,灰色预测模型GM(1,1)适用于食用菌价格预测,具有较高的准确度;预测2017年全国香菇、金针菇、双孢蘑菇、平菇和黑木耳年均价格分别为12.70、6.99、10.95、6.61、9.78元/kg;预测结果显示,未来香菇、平菇和黑木耳价格将上涨,双孢蘑菇价格保持平稳,金针菇价格会小幅度下降。     

Infection of human enterovirus 71 in immunosuppressed rhesus monkeys

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.     

河南新县野生油茶群体表型变异特征研究

为揭示河南新县野生油茶群体的变异特征,以新县7个典型野生油茶生长区的群体为研究对象,测定了油茶叶、花、果相关的15个性状,采用变异系数、相关性分析、聚类分析和方差分析等方法,分析油茶表型性状的多样性及群体间和群体内的变异特征。结果表明,新县野生油茶叶、花、果形态在群体内及群体间的表型多样性均较为丰富,7个群体间叶、花、果形态差异均达到极显著水平。7个群体内15个性状中,鲜籽质量变异程度最高(49.79%),其次是鲜籽数、单果质量和果皮厚,变异程度最低的是叶长(10.67%);聚类分析在欧式平均距离在阈值15处,将新县野生油茶群体分为了两大类,在不同程度上反映了各群体的特征。     

施磷对苜蓿光合产物在根茎叶的分配及抗蓟马的影响

为了明确施磷后紫花苜蓿根、茎、叶中光合产物分配与苜蓿抗蓟马能力的关系,本试验以紫花苜感虫品种‘甘农3号’和抗虫品种‘甘农9号’为材料,以北方苜蓿蓟马类优势害虫牛角花齿蓟马(Odontothripsloti)为研究对象,设0mg(P_2O_5)·kg~(-1)(土)、27mg(P_2O_5)·kg~(-1)(土)、54mg(P_2O_5)·kg~(-1)(土)、81mg(P_2O_5)·kg~(-1)(土)和108mg(P_2O_5)·kg~(-1)(土)5个磷水平,分别记为P0、P1、P2、P3和P4,在苜蓿6叶期,按3头·株~(-1)接入牛角花齿蓟马,分别于苜蓿持续受害7 d、14 d和21 d时,评价苜蓿的受害指数,测量单株叶、茎、根生物量和根、茎、叶中的可溶性糖和淀粉含量。结果表明:随着施磷水平的升高,‘甘农3号’和‘甘农9号’苜蓿的受害指数降低,总体以P3水平下最低;受害7 d时,两个苜蓿品种受害指数均下降但不显著;受害14 d和21 d时受害指数下降显著(P0.05)。施磷后苜蓿根、茎和叶生物量均显著增加,在蓟马为害前期(7d)和中期(14d)苜蓿受害较轻时,生物量向叶中分配较多;在受到持续较重的为害后(21d),苜蓿的生物量更多向根系分配,相应分配到叶部的生物量有所下降,茎秆中的生物量分配比例变化不显著。相对于‘甘农3号’,各施磷水平下‘甘农9号’分配到叶中的生物量更多。施磷后苜蓿根、茎和叶中可溶性糖和淀粉含量显著增加,随着受害时间的持续,苜蓿根、茎和叶中的淀粉含量总体下降,而可溶性糖含量持续增加;在受害14 d和21 d时,‘甘农9号’的叶和根中的可溶性糖及淀粉含量明显高于‘甘农3号’。总之,施磷可有效增强苜蓿对蓟马的耐害性,在虫害压力适中时,施磷促进了苜蓿地上部分的补偿生长,虫害压力较大时,施磷保证根系的生长以维持其生存。随着受害时间的持续,苜蓿存贮型碳水化合物淀粉的含量趋向减少,根、茎和叶中的可溶性糖含量升高,使较多的资源用于苜蓿光合器官和贮藏器官的构建。P3水平[81 mg(P_2O_5)·kg~(-1)(土)]为本试验中苜蓿最佳施磷水平。     

微信公共领域里的失范行为及其法治规范建设

从哈贝马斯公共领域理论出发，提出了微信群与微信公众号已经初步形成了公共领域这一观点，并主要介绍了围绕微信公共领域的社交功能、自媒体传播功能和商业贸易平台功能而产生的失范行为。依法规范微信公共领域，可以从完善依法管理微信公共领域、依法运营微信公共领域、依法使用微信公共领域三个有机联系层面的法治规范展开。     

Correlation between Mnk2 protein expression and prognosis of patients with esophageal squamous cell carcinoma

AIM: To investigate the protein expression of mitogen-activated protein kinase-interacting kinase-2 (Mnk2) and its prognostic effect in the patients with resected esophageal squamous cell carcinoma (ESCC). METHODS: A total of 86 informative patients with surgically resected ESCC and 54 normal esophageal tissues were enrolled. Western blot and immunohistochemistry (IHC) were utilized to assess the protein expression of Mnk2, and its correlation with prognosis was statistically analyzed by the methods of Kaplan-Meier curve and Cox proportional hazard mode. RESULTS: The protein expression of Mnk2 was elevated in most of tumor tissues compared with the adjacent tissues. Clinicopathologic analysis showed that Mnk2 expression was significantly correlated with the TNM stage (P<0.05). Both disease-free survival (DFS) and overall survival (OS) of Mnk2 over-expression patients were shorter than those in Mnk2 negative expression group. Multivariate analysis confirmed that Mnk2 expression, as an independent and significant factor for both DFS and OS, predicted a poor prognosis of the patients with resected ESCC (P<0.05). CONCLUSION: The expression of Mnk2 was significantly related to the TNM stages, and might be a novel predictor for prognosis in ESCC.     

转Bt基因抗虫棉对棉铃虫的抗性分析

用常规棉和不同品种的转Bt基因抗虫棉分别饲喂棉铃虫(Helicoverpa armigera),对棉铃虫幼虫生长发育过程中各生物学指标的变化进行室内观察分析。结果表明,不同品种的转Bt基因抗虫棉之间对棉铃虫幼虫体重和死亡率的影响程度由大到小依次为JK-1、DB、D16、新陆早36,另外,取食转Bt基因抗虫棉叶片之后棉铃虫幼虫的化蛹率比取食常规棉叶片的棉铃虫幼虫的化蛹率减少12.5%~30.0%,蛹重减轻15.6%~41.9%,羽化率降低5.5%~80.0%。     

Research progress on autophagy in treatment of glioma

Autophagy is a transport pathway from the cytoplasm to the lysosome, which is a major intracellular degradation/recycling system ubiquitous in eukaryotic cells. Autophagy regulation has achieved some gratifying results in the treatment of glioma. It is currently an exciting field of clinical development. In chemotherapy or radiotherapy, autophagy-related drugs are currently used in vitro and in vivo for treating tumors with significant effects. Autophagy inducers and inhibitors may potentially block tumor formation and enhance the anti-cancer immune response. A more comprehensive understanding of the role of autophagy in different stages of glioma development may guide the development of new therapeutic strategies.