椪柑优良新株系——东13选育报告

东13椪柑是1973年通过营养系选种,在广东省杨村华侨柑桔场选出的优良新株系。经过14年来系统的株系比较试验、中间试验及示范推广,证明该株系果大优质、果形高桩美观、早结丰产等性状稳定,综合性状优于目前生产上推广的品种(系)。该株系大面积出口货源基地已在广东省建立,省内外推广面积已超过8000亩。     

老鹰茶白粉病罹病植株的生理生化研究

供试的不同老鹰茶品种被病原菌侵染后，叶片中叶绿索含量、光合速率和蒸腾速率均随着感病程度的加剧而明显地下降，感病品种下降幅度大于抗性品种；而丙二醛含量随着感病程度的加剧而明显上升，感病品种增加的幅度比抗性品种大。老鹰茶叶片中叶绿素含量与其光合速率有显著的正相关关系。     

大白菜内因对小孢子胚胎发生能力的影响

以15个大白菜杂交种为试材,采用游离小孢子培养方法,研究基因型、花蕾长度对小孢子胚发生的影响。结果表明:不同试材的小孢子胚发生频率有明显差异,有11个品种诱导出胚状体,最高出胚率为每蕾54.17胚;诱导胚状体发生的最佳花蕾长度不同品种略有不同,多数品种在2.5～3.5mm时胚发生频率较高,其次是蕾长2.0～2.5mm时有较高发生频率。     

Real-time Infrared Image Acquisitionand Processing System Based on PCI Bus

In the system of infrared imaging acquisition, it is necessary to improve data transfer to satisfy the characteristics of high speed and real time. So,this paper introduces PCI bus and PCI inerface to imaging acquisition. The protocol of data transfer of PCI local bus and the structure of PCI9050 are studied in detail, and the IRFPA data acquisition system are described based on the protocol of data transfer of PCI local bus. The system adopts frame buffer to make slow transmission between peripheral circuit and PCI bus.CPLD is also used as a master of logic time control?     

北方寒地引种抗寒苜蓿品种试验

１９９１、１９９２年两年对１０个抗寒苜蓿品种进行了品种比较试验，鉴选出公农一号、草原一号、草原二号、甘农一号、图牧二号６个苜蓿良种。这些品种适应黑龙江垦区的生态条件，抗寒、抗病性强、长势好、产草量高、品质优良，可以在垦区三江平原推广种植。     

基于多层感知器神经网络对遥感融合图像和TM影像进行土地覆盖分类的研究

８０年代以来，人工神经元网络（ＡＮＮ）技术的应用不断向广度和深度发展．用不同空间分辨率的ＴＭ和ＩＲＳ遥感图像进行融合，综合了不同传感器数据所提供的信息，增强了图像的清晰度，改善了解译效果。用多层感知器神经网络对遥感融合图像进行分类，分类精度达９５％，比用多层感知器神经网络对ＴＭ图像进行分类（分类精度达７３％）效果要好．     

成年小鼠雄性生殖细胞的冷冻保存

在含10％小牛血清（NBS）的DMEM培养基中，分别各添加5％，10％，15％，20％和25％的二甲基亚砜（DMSO）,丙二醇（PG），乙二醇（EG）和甘油（G），对成年小鼠睾丸生殖细胞冷冻保存；复苏后台盼蓝染色测定细胞复苏率。结果显示，5％－25％DMSO冻存液组的细胞复苏率分别为88.5%,88.0%,65.6%及51.3%;5%-25%PG冻存液组的细胞复苏率分别为87.2%,86.4%,79.0%,73.4%及40.1%.;5%-25%EG冻存液组的细胞复苏率分别为6.6%,80.9%,60.8%,51.3%及30.0%;5%-25%G冻存液组的细胞复苏率分别为86．5％，86．3％，65．3％，36．0％及31．4％。其中各抗冻剂5％和10％组的细胞复苏率最高，与15％组相比均存在显著或极显著差异。4种抗冻剂的最高细胞复苏率之间无显著差异。DMSO，PG，EG和G分别冷冻保存成年小鼠睾丸生殖细胞对的最小损失率分别为4．8％，6．1％，6．7％，6．8％。结果表明，采用慢速冷冻时，DMSO，PG，EG及G均适宜用作成年小鼠睾丸生殖细胞的抗冷冻剂，最佳使用含量均为5％－10％。成年小鼠睾丸生殖细胞分别在含5％－10％的DMSO，PG，EG和G的DMEM（含10％NBS）冻存液中，2步慢速降温，液氮储存，37℃水浴复苏，是一种具有较高复苏率的冷冻保存方法。     

Preparation of recombinant PTD-HSP27 and verification of its ability to penetrate the cell membrane of human lens epithelial cells and rabbit cornea

AIM: To construct the prokaryotic expression system containing protein transduction domain (PTD) with heat shock protein 27 (HSP27) in order to prepare and purify the recombinant protein, and to verify whether the recombinant protein PTD-HSP27 has the ability to penetrate the human lens epithelial cell (HLEC) membrane and the rabbit cornea. METHODS: The plasmid pKYB-PTD-HSPB1-6His was constructed by the technique of overlap extension PCR. The plasmid was transformed and PTD-HSP27 was purified through nickel affinity chromatography column and identified by Western blotting. PTD-HSP27-6His was labeled with the fluorescein isothiocyanate (FITC). The penetrating ability of PTD-HSP27 into HLECs and rabbit cornea was tested. RESULTS: The recombinant PTD-HSP27 plasmid was successfully cloned and effectively expressed. The correctness of the recombinant protein PTD-HSP27 was demonstrated. Fluorescence microscopic examination showed that PTD-HSP27-FITC was internalized by HLECs. Fluorescent labeled PTD-HSP27 was then observed in the rabbit aqueous humor. CONCLUSION: The recombined gene PTD-HSPB1 was constructed by overlap extension PCR technique and the PTD-HSP27 fusion protein was prepared and purified by nickel affinity chromatography column. Using the technique of PTD-fusion protein, HSP27 was transduced into HLECs and passed through the cornea.     

NaCl和NaHCO_3胁迫对黑豆种子萌发的影响

以黑豆种子为试材,采用单盐胁迫的方法,对种子发芽率、发芽势、相对发芽率、活力指数、复萌率指标进行了测定分析;通过研究不同浓度NaCl和NaHCO3溶液胁迫对黑豆种子萌发的影响,探讨黑豆的耐盐碱能力。结果表明:黑豆的发芽率、发芽势、相对发芽率、发芽指数、活力指数、苗鲜重和胚根长随NaCl和NaHCO3溶液胁迫增加总体上呈下降趋势;苗鲜重和胚根长随着溶液胁迫的增长而下降,呈现显著的负相关关系,相对盐害率随溶液胁迫的增加呈现正相关关系;NaCl胁迫下黑豆种子萌发的耐盐浓度为18.253‰,半数抑制浓度为20.264‰,极限浓度为22.274‰;NaHCO3胁迫下黑豆萌发的耐盐浓度为18.316‰,半数抑制浓度为21.270‰,极限浓度为24.224‰;蒸馏水复萌试验表明,经NaCl胁迫后黑豆种子的复萌率均达到80%以上,黑豆种子经NaHCO3胁迫后复萌率均为0%;综上黑豆具有一定的抗耐盐碱能力。     

硼对小麦生殖器官形态及解剖结构的影响

用溶液培养方法研究硼对小麦生殖器官形态及解剖结构的影响. 结果表明: (1)缺硼严重影响小麦穗的分化, 在一定施硼范围内, 总小穗数和有效小穗数随硼施用量增加而增多; (2)缺硼严重影响小麦雄蕊发育. 无硼(B0处理)条件下, 少数雄蕊不发育, 多数虽发育, 但体积极小, 造孢细胞有丝分裂阶段出现异常, 细胞内有多核现象, 减数分裂