布鲁氏菌OMP16对RAW264.7细胞凋亡与免疫活性的影响

旨在研究布鲁氏菌外膜蛋白OMP16对小鼠巨噬细胞RAW264.7细胞凋亡和免疫活性的影响及其机制探讨。以OMP16处理RAW264.7细胞为模型,通过MTT以及Hoechst法检测OMP16对细胞活力的影响,通过Western blot检测Caspase-3、GRP78、CHOP的表达情况,流式细胞术检测OMP16对细胞凋亡的影响以及RT-PCR检测免疫因子IL-1β、IL-6和TNF-α的变化。结果表明,OMP16能够影响RAW264.7细胞的活力,且有浓度依赖性;添加OMP16后能够显著增加凋亡标志因子Caspase-3的表达（P<0.001）,并促进RAW264.7细胞的凋亡;作用24、36 h之后,显著引起内质网应激标志蛋白GRP78、CHOP的表达;并且能显著上调IL-1β、IL-6、TNF-α等炎性因子的mRNA表达水平。布鲁氏菌OMP16能够诱导RAW264.7凋亡,OMP16显著引起内质网应激CHOP通路的激活。     

沙棘资源开发利用应注意的问题

文章介绍了沙棘资源概况以及全国沙棘资源开发利用的现状,阐述了沙棘开发利用中应注意的问题。     

中/高密度纤维板加工工艺及新技术开发--加拿大国家林产工业技术研究院MDF/HDF实验中心侧访

加拿大森林资源丰富,林产工业是其重要产业之一,2004年其MDF产年量达157万m3,约占北美地区MDF年总产量的30%,并且近年来一直保持着稳步的发展态势.加拿大国家林产工业技术研究院(Forintek)是该国唯一的国家林产工业研究机构,对加拿大林产工业的发展起到举足轻重的作用.Forintek总部位于温哥华(Vancouver)市,并在魁北克(Quebec)省Sainte-Foy市设有一个研究分院,在渥太华(Ottawa)等其他主要省/市还设有多个办事处,便于与工厂加强联系.为支持、适应北美地区MDF/HDF的发展,Forintek在Quebec研究分院专门成立了一个MDF/HDF实验中心.获悉2005年秋,我国人造板行业的知名专家--东北林业大学陆仁书教授应邀前往Quebec分院访问.笔者慕名就该院MDF/HDF实验中心的运作及研究状况采访了陆教授,并作此篇报道,以飨读者.     

桂花品种切花瓶插衰老生理研究

研究了不同桂花品种切花在开花和衰老过程中花朵的保护酶活性、丙二醛(MDA)、细胞质膜透性及可溶性蛋白质等的变化规律.结果表明:3个桂花品种花朵的可溶性蛋白质的含量均呈下降趋势,细胞质膜透性和丙二醛含量随花朵的衰老而增大;超氧化物歧化酶(SOD)活性前期上升后期下降,呈现出明显的峰值;过氧化物酶(POD)表现为上升趋势.综合比较各项指标测定结果,3个品种的瓶插寿命长短为:'晚籽银'桂＞'多芽金'桂＞'四季桂'.保鲜液对延长桂花花期有一定的效果.     

广汉市行道树树种的选择与配置模式

本文调查了四川省广汉市行道树的现状,该市行道树主要以黄葛树(Ficus lacor)和垂柳(Salix babylonica)为主,种类单调,道路绿化覆盖率极低。因此,在对城市绿地系统进行规划时,提出了行道树种的选择原则,并规划了桢楠(Phoebe zhennan)、香樟(Cinnamomum camphora)、广玉兰(Magnolia grandiflora)、阴香(Cinnamomum burman-ni)、银木(Cinnamomum septentrionale)等8种为基调树种,雪松(Cedrus deodara)、栾树(Koelreuteria paniculata)、鹅掌楸(Liriodendron chinense)、蓝花楹(Jacaranda acutifolia)、峨眉含笑(Magnolia wilsonii)、银杏(Ginkgo biloba)、金丝垂柳(Salixsp.)等19种为骨干树种。根据行道树种的主导功能目标,提出了行道树的配置模式,如观赏型、环保型和文化休闲型等,以体现城市区域特色。     

湖南黄丰桥林场杉木树高曲线模拟研制

树高曲线在森林经营和收获调整中具有重要的作用。用湖南省黄丰桥林场40块杉木样地资料,对10个非线性树高曲线模型进行了拟合和检验。结果表明:Kozak函数是模拟树高曲线的理想模型。经检验,建立的杉木人工林树高预测方程可在研究区内应用。     

水蛭素的全基因合成及克隆

报道了水蛭素全基因的合成及克隆。根据已知水蛭素氨基酸序列及酵母菌体内蛋白质表达特性,设计了水蛭素的全基因序列,采用化学合成及酶学相结合的方法,合成了该基因片段,并逐段克隆至载体ｐＢＳ上,得到全长水蛭素基因。序列测定表明,克隆后ＤＮＡ顺序同原设计序列。     

Research of Application About Environment Dredging on River and Reservoir with Water Jet Airlift

Being established in the thought of environment dredging, the crafts flow of water jet airlift dredging and treating of surplus water as well as circle using of mud polluted have been analysed following the status in quo of pollution of the fiver and reservoir. Definitely, it has been pointed out that the total of mud will be reduced with the technique of dehydration so as to reduce the volume of mud stack. At the same ring the surplus water. The mud which had been treated can be time, it will be reduced for pollution secondly by treaused as soil of fiver or gardens or road for the recycle. Then, the parameter and efficiency of production in HuaXi river valley integration treating project have been expatiated particularly in environment dredging. It indicates the new way for environment dredging in river and reservoir at the mountainous area.     

Implications of Serial Analysis of Gene Expression for Cancer Research

Serial analysis of gene expression (SAGE) is a powerful technique designed to analyze the information of gene expression. It can not only immediately analyze thousands of genes in detail, but also find new genes. Therefore SAGE is an effective tool of qualitative and quantitative research for gene expression. Recently the SAGE method is widespread applied in cancer research, understanding pathways involved in tumorigenesis and identifying novel diagnostic and therapeutic targets. It is implied that SAGE in implications for cancer research, diagnostic and targeting process will have a great impact on cancer research.     

The Influence of World Trade Organization Agreement on Government Procurement Public Project Purchasing by Invitation to Bid

Based on the presentation of the general principles, the scope of application and the purchasing modes of the World Trade Organization Agreement on Government Procurement, in this paper, its influence on the public project purchasing by invitation to bid after China's entrance into Government Procurement Agreement is analyzed. Some feasible proposals for the development of domestic public project purchasing by invitation to bid are presented.