Hot water immersion lowers survival,shell growth rate and lysosomal membrane stability of oysters Crassostrea virginica (Gmelin)

This study compares the effect of two anti‐fouling treatments, hot water immersion (15 s at 60°C) and air drying (72 h) on the physiological status of the Eastern oyster Crassostrea virginica. The negative impact of hot water immersion was greater than that of air drying, but varied depending on the initial size of the oysters (40 vs. 60 mm shell height) and the time of the year (June vs. August). Groups treated with hot water exhibited a higher proportion of haemocytes with destabilized lysosomal membranes (HDLM; 47.5 ± 3.1%) than those exposed to air drying (37.5 ± 2.9%). This suggests that the oyster immunocompetency may be lowered by hot water immersion. Overall, the large oysters had lower HDLM values (32.9 ± 3.5%) than the small individuals in June (45.7 ± 2.8%) but similar values in August (46.6 ± 3.5%). Small oysters subjected to hot water immersion in June exhibited a 50% reduction in shell growth and a 50% mortality rate after one month. Our results indicate that air drying is more suitable than hot water immersion as an anti‐fouling treatment for <45 mm oysters.     

Characterization of some Middle European soil textures by gamma‐spectrometry

Information about the variability of different soil attributes within a field is essential for sustainable land management and precision agriculture. Mobile proximal gamma‐ray spectrometry can map soil characteristics of vast areas at different scales rapidly and cost‐effectively. This study aims at investigating reliability and capability of mobile‐gamma‐spectrometry (radiometrics) data to map typical soils of Middle Europe. In this paper, we investigate relationships between the radioelement concentrations (K, U, Th, and dose rate) and soil parameters (texture, CEC, pH, and organic‐C content) at four different field sites and soil textures. The data reliability is confirmed at the survey start. Mobile data have an excellent linear correlation (nearly 1:1) with the stationary readings (of identical devices, acquisition setups, and soil conditions) but moderate correlation with laboratory data (of different devices, setups, and sample conditions). Dried lab samples have systematically higher radioelement concentrations than the field soils (normally wet). Consequently, the mobile‐gamma‐spectrometric data is sufficiently accurate for soil mapping, and its calibration by laboratory data is less useful due to the varying environmental conditions. Single absolute radioelement concentrations show only moderate correlations with the different soil parameters, particularly clay content and CEC. This may be related to varying environmental conditions (soil moisture, soil structure, vegetation, land use, etc.) between the study sites. Investigations of the ratios of radioelement concentrations yield a clear improvement of their correlations to soil parameters, especially for sand and clay contents, CEC, and organic C. Additionally, multiple‐linear‐regression models were established using the element concentrations of potassium and thorium to predict silt content and pH. The results of the highly correlated models were confirmed by comparing with clay and silt content and pH value, respectively, to six additional independent field samples. Briefly, applications of gamma‐ray data for soil mapping offers the possibility of the development of quantitative relationships regarding soil parameters like sand and clay contents, CEC, and organic C. Classification of soil textures by gamma‐ray data seems to be promising, though a broader database of soils is needed for further research. We recommend gamma‐ray mapping as a complementary or even an alternative to common mapping techniques.     

Pathogenicity and infectivity of Phytophthora ramorum vary depending on host species,infected plant part,inoculum potential,pathogen genotype,and temperature

A total of 25 ornamental plant species representing 10 families were inoculated using three genotypes, each representing one of the genetic lineages NA1, NA2, and EU1 of the pathogen Phytophthora ramorum. Leaves were inoculated using suspensions with two zoospore concentrations and exposure at three temperatures, while stems were inoculated using agar plugs colonized by mycelia. Susceptibility was determined by measuring either the success of pathogen reisolation or lesion length caused by the pathogen. Infectivity was determined by counting sporangia in washes of inoculated leaves or stems. Results from all three pathogen genotypes combined were used to rank each of the 25 plant species for susceptibility and infectivity, while pooled results per genotype from all 25 hosts combined were employed for a preliminary comparison of pathogenicity and infectivity among genotypes. Statistical analyses showed that leaf results were affected by the concentration of zoospores, temperature, plant host, pathogen genotype, and by the interaction between host and pathogen genotype. Stem results were mostly affected by host and by the interaction between host and pathogen genotype. Hosts ranked differently when looking at the various parameters, and differences in rankings were also significant when comparing stem and leaf results. Differences were identified among the 25 hosts and the three pathogen genotypes for all parameters: results can be used for decision-making regarding regulations or selection of plants to be grown where infestations by P. ramorum are an issue.     

An object-oriented Bayesian network modeling the causes of leg disorders in finisher herds

The implementation of an effective control strategy against disease in a finisher herd requires knowledge regarding the disease level in the herd. A Bayesian network was constructed that can estimate risk indexes for three cause-categories of leg disorders in a finisher herd. The cause-categories of leg disorders were divided into infectious causes (arthritis caused by infectious pathogens), physical causes (e.g. fracture and claw lesions), and inherited causes (osteochondrosis). Information about the herd (e.g. the herd size, floor type and number of suppliers) and information about individual pigs (e.g. results from diagnostic tests) were used to estimate the most likely cause of leg disorders at herd level. As information to the model originated from two different levels, we used an object-oriented structure in order to ease the specification of the Bayesian network. Hence, a Herd class and a Pig class comprised the basic components of the object-oriented structure. The causal structure of the model was based on evidence from published literature. The conditional probabilities used in the model were elicited from experts within the field and from the published literature. To illustrate the behaviour of the model, we investigated the value of different levels of evidence in two fictitious herds with different herd characteristics related to the risk of leg disorders (e.g. purchase policy, production type and the stocking density in pens). The model enabled us to demonstrate the value of performing systematic collection of additional information (i.e. clinical, pathological and bacteriological examination) when identifying causes of leg disorders at herd level.     

Netrins promote developmental and therapeutic angiogenesis

Axonal guidance and vascular patterning share several guidance cues, including proteins in the netrin family. We demonstrate that netrins stimulate proliferation, migration, and tube formation of human endothelial cells in vitro and that this stimulation is independent of known netrin receptors. Suppression of netrin1a messenger RNA in zebrafish inhibits vascular sprouting, implying a proangiogenic role for netrins during vertebrate development. We also show that netrins accelerate neovascularization in an in vivo model of ischemia and that they reverse neuropathy and vasculopathy in a diabetic murine model. We propose that the attractive vascular and neural guidance functions of netrins offer a unique therapeutic potential.     

Chemical and biological assessment of a chinese herbal decoction containing Radix Astragali and Radix Angelicae Sinensis: Determination of drug ratio in having optimized properties

Danggui Buxue Tang (DBT), a Chinese medicinal decoction that is commonly used as a dietary supplement in treating woman with menopausal irregularity, contains two herbs: Radix Astragali (Huangqi) and Radix Angelicae Sinensis (Danggui). The ratio of Radix Astragali and Radix Angelicae Sinensis used in DBT should be 5:1 as described in China in 1247 A.D.; however, the rationale of this formula has not been given. Here, the chemical and biological properties of DBT, prepared from different ratios of the drugs, were determined. Significantly, higher amounts of Radix Astragali-derived astragaloside IV, calycosin, and formononetin and Radix Angelicae Sinensis-derived ferulic acid were found in DBT with Radix Astragali and Radix Angelicae Sinensis in a 5:1 ratio. By using the biological effects of DBT in stimulating osteoblast proliferation, estrogen promoter activation, and anti-platelet aggregation activity, the drug ratio of 5:1 produced the best effects. In addition, the use of ethanol-treated Radix Angelicae Sinensis enhanced the efficacy of DBT, and the treatment further increased the solubilities of chemical constituents. By analyzing the correlation of chemical and biological results, several chemicals showed positive correlation with DBT-induced bioactivities. The current results support the ancient formulation of DBT, and the identified chemicals could serve as markers for quality control of DBT.     

Iron supplement use among Danish pregnant women

OBJECTIVES: To evaluate compliance with the national recommendation on supplemental iron to all pregnant women in Denmark and to explore differences between compliers and non-compliers with respect to dietary habits and other lifestyle factors. DESIGN: Intake of supplemental iron from pure iron supplements and from multivitamin and mineral preparations was estimated in mid-pregnancy. SETTING: Nationwide cohort study, the Danish National Birth Cohort (DNBC), comprising more than 100,000 women recruited in early pregnancy. SUBJECTS: Information on diet and dietary supplements was available for 54,371 women. Of these, information on lifestyle factors was available for 50,902 women. RESULTS: A high compliance with the recommendation was found, as approximately 77% of the women reported use of iron supplements during pregnancy. However, many of the compliers did not obtain the recommended doses of iron, which can partly be explained by the lack of iron preparations of appropriate doses available on the Danish market. Compliance with the recommendation was associated with age above 20 years, primiparity, body mass index<30 kg m- 2, non-smoking and long education. No major differences were seen in dietary intake between compliers and non-compliers.ConclusionOverall, a high compliance rate was found among participants of the DNBC but a clarification on daily dose is needed, and more concern should be paid to vulnerable groups such as young, smoking women and women with no or short education.     

Glacial survival of boreal trees in northern Scandinavia

It is commonly believed that trees were absent in Scandinavia during the last glaciation and first recolonized the Scandinavian Peninsula with the retreat of its ice sheet some 9000 years ago. Here, we show the presence of a rare mitochondrial DNA haplotype of spruce that appears unique to Scandinavia and with its highest frequency to the west-an area believed to sustain ice-free refugia during most of the last ice age. We further show the survival of DNA from this haplotype in lake sediments and pollen of Tr?ndelag in central Norway dating back ~10,300 years and chloroplast DNA of pine and spruce in lake sediments adjacent to the ice-free And?ya refugium in northwestern Norway as early as ~22,000 and 17,700 years ago, respectively. Our findings imply that conifer trees survived in ice-free refugia of Scandinavia during the last glaciation, challenging current views on survival and spread of trees as a response to climate changes.