牛腐蹄病坏死梭杆菌白细胞毒素重组亚单位疫苗诱导小鼠免疫保护效果的观察

以大肠杆菌表达系统表达并纯化的牛源坏死杆菌H05菌株5个截短的重组白细胞毒素蛋白PL1（1aa～174aa）、PL2（311aa～644aa）、1ktA3（1319aa～2026aa）、PL4（1960aa～2287aa）和PL5（3077aa～3241aa）为抗原分别免疫昆明系小白鼠,同时将灭活的天然坏死杆菌白细胞毒素和PBS作为免疫对照。ELISA结果显示,经4次免疫后5个重组蛋白和天然白细胞毒素均能介导小鼠产生抗白细胞毒素蛋白的特异性抗体,抗体效价分别为1：6400、1：12800、1：6400、1：12800、1：3200和1：800。免疫鼠活菌攻击观察结果和免疫鼠肝脏组织学变化结果一致证明,PL1、PL2、1ktA3、PL4、PL5重组蛋白和天然白细胞毒素对小鼠均具有保护作用,5个重组蛋白的免疫保护作用均优于天然白细胞毒素,在5个重组蛋白中PL1、1ktA3和PL4对鼠的免疫保护效果最好。     

猪瘟免疫猪接种PRRSV Nsp2Δ1882-2241弱毒疫苗后免疫应答及T细胞表型变化特征

选择17头28日龄的CSFV和PRRSV抗体均为阴性的仔猪,于试验的第1天和第14天分别对其进行猪瘟耐热保护剂活疫苗（兔源）和高致病性猪繁殖与呼吸综合征Nsp2A1882—2241弱毒疫苗免疫。在免疫后的第28、42天采集外周血液,分析特异性抗体表达量和外周血T淋巴细胞表型的变化,评估猪瘟免疫对猪繁殖与呼吸综合征免疫的影响。结果显示,在CSF免疫后第28、42天,CSFV高抗组中的CD4^＋、CD4^＋／CD8^＋、CD4^＋CD8^＋和CD4-CD8数目均比CSFV低抗组高,CD3^＋和CD8^＋细胞数量比CSFV低抗组低;PRRS高抗组中,CD4CD8-细胞含量高于PRRS低抗组;在CSF免疫后第28天,CSFV抗体产生较高（阳性比率为73．33％）,PRRSV抗体产生较低（阳性比率仅为6．67％）。在CSF免疫后的第42天,CSF高抗组中PRRSV抗体阳性比率较CSF低抗组高8．33％。结果表明,CSFV特异性抗体产生高时能增加PRRSV特异性细胞免疫应答,增加CD4^＋细胞、CD4^＋CD8^＋细胞数量,提高机体免疫水平。CD3＋和CD4CD8-细胞应答作用值得重视。     

不孕症奶牛抗卵巢抗体的检测及临床意义

为探讨奶牛不孕症与ＡＯＡｂ的关系,用放免法检测啊１４例原发性不孕、１０例继发性不孕奶牛血清ＡＯＡｂ,并与正常妊娠组对照。实验表明,ＡＯＡｂ阳性１０例,总阳性率为４１．６７％,ＡＯＡｂ为原发性不孕相关,与继发性不孕无明显关系。结果提示,ＡＯＡｂ的存在是奶牛原发性不孕的重要病因。     

几种环保型饲料添加剂对欧洲鳗鲡消化酶活性的影响

选取健康均匀的欧洲鳗苗1000尾,分为5组,每组2个重复,分别在基础料中添加4种环保型饲料添加剂,分析了4种环保型饲料添加剂对欧洲鳗鲡消化酶活性的影响。结果表明,1号饲料添加剂对于提高欧洲鳗鲡消化酶整体效果最好,对中草药原料的配伍进行选择优化,适当添加半胱胺,能显著提高欧洲鳗鲡幼鱼消化酶活性。     

Characterization of turkey coronavirus from turkey poults with acute enteritis.

The present study was to characterize turkey coronavirus associated with turkey poult enteritis and mortality. Intestinal contents or intestines from affected turkey poults and inoculated turkey embryos contained coronaviruses as revealed by electron microscopy or were positive for turkey coronavirus by immunofluorescent antibody assay. Sucrose density gradient ultracentrifugation of the virus-containing intestinal homogenate yielded two opalescent bands corresponding to the buoyant densities of 1.14-1.15 and 1.18-1.20 g/ml, respectively. Coronaviral particles from intestinal contents or the sucrose density gradient preparation were mainly spherical in shape and had envelope and central depression. They were surrounded by a fringe of regularly spaced petal-shaped projections attached to the particles by a short stalk. Purified viruses hemagglutinated rabbit erythrocytes with a titer of 16. Major protein bands of purified viruses analyzed by SDS-PAGE were located at 200, 100-110, 50-60, and 30-35 kDa. The patterns of protein bands were consistent with those of Minnesota or Quebec turkey coronavirus isolates. A 568 bp nucleotide fragment of turkey coronavirus spike protein gene was amplified from RNA of inoculated turkey embryo intestine or purified virus. Sequence analysis of the 568 bp PCR product revealed high degree of identity with the corresponding spike protein gene sequence of human and bovine coronaviruses. The results indicated that turkey coronavirus was associated with turkey poults with acute enteritis.     

畜禽热应激的营养防制措施探讨

<正>夏季高温造成畜禽热应激给畜牧业生产带来巨大损失,降低环境温度是缓解热应激最有效的方法,但在实际生产中因不可行或成本太高而难以应用。因     

Electroacupuncture-induced neural activation detected by use of manganese-enhanced functional magnetic resonance imaging in rabbits

OBJECTIVE: To investigate the effects of acupuncture on neural activity detected by use of manganese-enhanced functional magnetic resonance imaging (fMRI) and elucidate the relationship between somatic acupoint stimulation and brain activation. ANIMALS: 40 New Zealand White rabbits. PROCEDURE: Manganese-enhanced fMRI was performed in anesthetized rabbits manipulated with electroacupuncture (EA) on Zusanli (ST-36) and Yanglingquan (GB-34) acupoints. Image acquisition was performed on a 1.5T superconductive clinical scanner with a circular polarized extremity coil. T1-weighted images were acquired sequentially as follows: baseline, after mannitol injection, after manganese infusion, and 5 and 20 minutes after initiation of EA. RESULTS: Changes in focal neural activity were detected by use of manganese-enhanced fMRI. Stimulation on Zusanli (ST-36) for 5 minutes resulted in activation of the hippocampus, whereas stimulation on Yanglingquan (GB-34) resulted in activation of the hypothalamus, insula, and motor cortex. Activation became less specific after 20 minutes of EA. Furthermore, stimulation on ipsilateral acupoints led to bilateral brain activation. CONCLUSIONS AND CLINICAL RELEVANCE: Each acupoint has a corresponding cerebral linkage, and stimulation on these points resulted in time-dependent neural activation. Understanding the linkage between peripheral acupoint stimulation and central neural pathways may provide a useful guide for clinical applications of acupuncture.     

“赛豹”追踪侦破女尸案

2010年以来,云南红河州个旧市一些不法分子大肆对锡矿石进行盗抢,给国家造成重大经济损失。为此,个旧市委、市政府决定对矿山进行一次专项整治,制止大规模集体盗抢违法行为的发生,个旧市公安局于3月12日抽调了4头警犬进驻治安较为复杂的1800矿区参与治安专项整治,掀开了矿山整治的序幕。目前,1800矿区的盗抢案件大幅度下降,警犬在专项整治中发挥了重要作用,收到了良好成效。     

辐射诱变育种在牧草和草坪草中的应用

对辐射诱变育种的概念、主要类型及特点、诱变材料的选择、诱变的程序及其在牧草、草坪草方面的应用研究情况进行综合叙述.