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31.
This study evaluates the influence of temperature manipulation on circulating levels of sex steroids and gonadal maturation in F1 generation Senegalese sole Solea senegalensis breeders kept under either continuous light (LL) or natural photoperiod (NP). Under LL, progressive water temperature reduction during early winter [treatment MT (modified temperature)] significantly elevated [compared with exposure to LL and constant temperature (CT)] plasma steroid levels in females (January–February) and males (January) and proportion of females initiating maturation (January), suggesting a modulatory role of decreasing temperature in the early phases of sole gonadal recrudescence. Under NP, treatment MT did not stimulate female gonadal maturation despite the significantly higher plasma testosterone levels (February) compared with exposure to NP and CT, which may indicate that females at early‐intermediate phases of ovarian development, the predominant stages of maturation at early winter, were less responsive to the treatment. Conversely, treatment MT significantly enhanced male androgen production from January onwards, together with an increase in proportions of running individuals (March), indicating that males were also responsive to this treatment at mid‐late phases of testicular development. Our data further support the strong influence of water temperature on the overall control of gonadal maturation in Senegalese sole. 相似文献
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Mizuki TOMIHARI Akira NISHIHARA Terumasa SHIMADA Masashi YANAGAWA Masafumi MIYOSHI Kazurou MIYAHARA Akihiro OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1227-1233
Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity,
and research shows that total intravenous anesthesia (TIVA) with propofol has a greater
immuno-protective effect than inhalational anesthesia in human medicine. However, in
veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on
propofol and isoflurane, investigating clinical blood hematology and immunological
profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy
adult beagles were included in this study, randomly assigned to the propofol anesthesia
group (group P: n=6) or the isoflurane anesthesia group (group I: n=6). In both groups,
the number of lymphocytes in peripheral blood decreased after 2 hr of anesthesia (2 hr),
but group P showed significantly less decrease than group I. For T-lymphocyte subsets
examined by flowcytometry, the ratio of CD3+, CD4+ and CD8+ lymphocytes in the peripheral
blood mononuclear cell (PBMC) of group P at 2 hr also exhibited a high level compared to
group I. Moreover, for mRNA expression of cytokines measured by real-time PCR, the IL2
(pro-inflammatory cytokine) of group P showed no decrease like group I. The IL10
(anti-inflammatory cytokine) of group P also showed no increase like group I, while both
cytokines maintained nearly the same level until 2 hr. These results suggest that,
compared to propofol, isoflurane had more strongly immuno-suppression caused by
anesthesia, and propofol itself might have some immuno-protective effects. Thus, TIVA with
propofol might benefit immunological support in the perioperative period of dogs. 相似文献
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Mizuki KURAMOCHI Takeshi IZAWA Mayuka HORI Kayo KUSUDA Junichiro SHIMIZU Toshie ISERI Hideo AKIYOSHI Fumihito OHASHI Mitsuru KUWAMURA Jyoji YAMATE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):893-895
A 19-year-old female Bengal tiger (Panthera tigris tigris) was presented
with hind limb weakness, ataxia and respiratory distress. Computed tomography revealed a
mass between the left side of the T7 vertebra and the base of the left 7th rib. The tiger
then died, and necropsy was performed. Grossly, the vertebral mass was 6 × 5.7 × 3 cm, and
invaded the adjacent vertebral bone and compressed the T7 spinal cord. Histologically, the
mass was composed of large, clear, vacuolated and polygonal cells with osteochondral
matrix. Cellular and nuclear atypia were moderate. The vacuolated cells stained positively
for cytokeratin and vimentin and negatively for S-100. Based on these findings, the
present case was diagnosed as a vertebral chordoma; the first report in a tiger. 相似文献
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Ohnishi M Sawada T Hirose K Sato R Hayashimoto M Hata E Yonezawa C Kato H 《Veterinary microbiology》2011,154(1-2):202-207
The presence of metallo-β-lactamase (MBL)-producing and multidrug-resistant Pseudomonas aeruginosa (MDRP) strains among bovine isolates of Gram-negative bacilli, and O-serotypes of bovine Serratia marcescens and P. aeruginosa isolates have been reported rarely. The aims of this study were to (1) elucidate antimicrobial susceptibilities and O-serotypes of P. aeruginosa and S. marcescens isolates from bovine mastitis and the presence of MBL-producers and MDRP strains among them and (2) evaluate their relationships to human isolates. We investigated the MICs of 24 antimicrobials and O-serotypes for 116 P. aeruginosa and 55 S. marcescens isolates in Japan, primarily in 2006. A total of 171 isolates exhibited high antimicrobial susceptibilities with the exception of a partial drug. P. aeruginosa isolates exhibited high susceptibilities of ≥ 95.7% to ciprofloxacin, imipenem, meropenem, piperacillin, ceftazidime, cefepime, cefoperazone/sulbactam, amikacin, tobramycin, and gentamicin; however, they exhibited a susceptibility of only 69.8% to aztreonam. They exhibited substantial resistances to ceftriaxone, enrofloxacin, cefotaxime, and moxalactam. S. marcescens isolates exhibited high susceptibilities of ≥ 90.9% to kanamycin, ceftiofur, sulfamethoxazole-trimethoprim, and the 15 aforementioned drugs, but exhibited resistance to minocycline. Neither MBL-producers nor MDRP strains were detected among the 171 strains. The dominant serotypes of P. aeruginosa isolates were OG, OA, OB, OI, OF, OE, and OK; those of S. marcescens isolates were O6 and O5. Every S. marcescens isolate was pigmented. These findings suggest that bovine P. aeruginosa and S. marcescens isolates differ from human isolates from both antibiogram and phenotypic perspectives, and could help to evaluate differences in bacteriological characteristics between bovine and human isolates. 相似文献
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This study examined the diversity of siderophore‐producing bacteria in the intestinal tracts of coastal fish in Japan and screened candidate bacterial strains for probiotic use in aquaculture. Of the 2637 bacteria isolated from the 27 fish specimens (13 species) and six environmental samples collected in this study, 266 isolates exhibited the ability to produce siderophores. Siderophore producers were detected in the intestines of 18 of the fish specimens caught (68%) at densities of 2.3 × 104–2.3 × 108 CFU g?1, in all three seawater samples at 2.0 × 102–1.3 × 103 CFU mL?1 and in all three sand samples at 2.6 × 101–2.8 × 104 CFU g?1. These findings suggest that siderophore‐producing bacteria are widely distributed in the intestinal tracts of coastal fish and their environments. Analysis of 16S rRNA gene sequences revealed that the siderophore producers belonged to 38 species, of which Vibrio splendidus, Vibrio ichthyoenteri and Vibrio crassostreae accounted for 32.7%, 19.5% and 11.3% of the 266 isolates, respectively, suggesting that these bacteria are indigenous to the intestinal tract of coastal fish. Six bacterial species, Enterovibri norvegicus, Photobacterium leiognathi, Photobacterium phosphoreum, Photobacterium rosenbergii, V. crassostrea and Vibrio scophthalmi were identified as possible candidates for use as probionts in fish aquaculture. 相似文献
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Mito Kokawa Kaori FujitaJunichi Sugiyama Mizuki TsutaMario Shibata Tetsuya ArakiHiroshi Nabetani 《Journal of Cereal Science》2012,55(1):15-21
A method combining fluorescence fingerprint (FF) measurement with an imaging technique was developed to visualize the distributions of gluten and starch in dough. The fluorescence images of dough at the under-, optimum- and over-mixing stages were acquired in multiple excitation and emission wavelengths constructing the FF. This data containing the FF data of each pixel in the sample image was colored according to the similarity of the FF of each pixel and the FF of gluten or starch. Furthermore, the areas corresponding to air bubbles in the dough were masked in black. The resulting image, referred to as the FF pseudocolor image, showed the distributions of gluten and starch in the dough with the incorporated bubbles. Quantitative parameters concerning gluten and starch distributions and bubble area were extracted from the dough images at each mixing stage, showing the homogenization of gluten and starch and the incorporation of bubbles with mixing. This imaging method is suggested to have an advantage over conventional imaging methods since there is no need to preprocess the sample, and any constituent in the sample can be visualized as long as there is information about the FF of the pure target constituent. 相似文献