成纤维上皮细胞炎症反应模型的建立

利用培养金黄色葡萄球菌培养基过滤液建立成纤维上皮细胞炎症反应模型，用于观察和研究炎症反应过程中细胞形态的变化。将培养金黄色葡萄球菌培养基除菌过滤冻干，用细胞培养基稀释，按照一定蛋白含量加到上皮细胞的培养基中，制作细胞炎症反应模型。结果显示，成纤维上皮细胞发生炎症反应，高浓度细菌滤过液的细胞形态发生变化，细胞边缘模糊不清，细胞出现空泡，随着培养时间的延长低浓度的大量大型细胞开始增加；在添加金黄色葡萄球菌培养基滤过液培养24h后利用^3H—TdR方法测定细胞增殖率，细胞增殖差异是显著的。结果表明，建立细胞炎症反应模型是可行的，并为以后炎症反应机理研究和抗炎性反应药物研究奠定了基础。     

坳陷湖盆斜坡带曲流河砂体分布与隐蔽油藏预测——以陕北宁条梁-乔沟湾地区延安组延91油组为例

认识鄂尔多斯盆地陕北斜坡延安组砂体展布与隐蔽油藏分布是加快该区油气勘探与开发步伐面临的重要问题之一。以陕北斜坡宁条梁-乔沟湾地区延安组延91油组为例,利用岩芯、露头、测井、录井和分析化验资料,研究了斜坡带曲流河沉积微相展布与砂体分布规律,应用地质综合评价法,预测与评价了有利目标。结果表明,该区延91油组主要有曲流河河道、河漫滩和决口扇等3种沉积微相,发育多条主河道,呈北东-南西方向展布;砂体厚度4～20m,呈条带状分布,与沉积微相平面展布基本一致,在纵向上连续性好,而在横向上连续性相对较差,河道砂体孔隙较发育,渗透率较高;Ⅰ类目标2个,预测含油面积为22.91km2,地质储量为903×104t;Ⅱ类目标5个,预测含油面积为57.47km2,地质储量为2654×104t;Ⅲ类目标7个,预测含油面积为103.28km2,地质储量为3545×104t。砂体这种分布模式对预测隐蔽油藏分布有重要指导意义,下一步勘探重点应在曲流河河道砂体带上寻找隐蔽油藏。     

植物小热激蛋白及其生物学功能研究进展

植物中的小热激蛋白(sHSPs)含量丰富,种类繁多。除高温外,sHSPs还被其他多种胁迫诱导,亦能受植物正常发育过程调控。sHSPs由核基因编码,其典型特征是有1个保守的α-晶状体蛋白域结构。通常植物sHSPs具有分子伴侣功能,在不依赖ATP的情况下阻止变性蛋白的聚集,从而对植物抵抗、适应胁迫环境以及生长发育起着重要作用。文章对植物中sHSPs的种类、蛋白结构特征和生物学功能进行了综述。     

广州城郊环境梯度下马尾松针叶元素质量分数变化特征

城市化、工业化迅速发展及人类活动的加剧给城市及其周边地区森林生态系统带来前所未有的干扰和压力。植物冠层是植物与大气直接进行气体交换的界面,冠层叶组织化学分析已成为评价环境污染对森林树木健康的影响,指示环境污染的有效手段。文章以广州黄埔工业区（丹水坑风景区）、城郊接壤区（华南植物园）和近郊（帽峰山森林公园）这一环境梯度为对象,在各研究点上选定自然生长的、成熟健康的马尾松（Pinus massonianaL.）5株,分别采集树冠中部向阳枝条上的当年生（Current,C）和一年生（Current＋1,C＋1）针叶,然后将每组针叶区分为带叶鞘针叶、去叶鞘针叶和叶鞘3个组分进行样本制备,测定N、Al、Cu、Cd、Pb、Zn、Cr、Ni元素质量分数,旨在探讨利用马尾松针叶不同组分的元素质量分数用于指示城郊梯度环境质量变化的适宜性和可靠性。研究结果表明：①当年生带叶鞘针叶中Al质量分数显著高于当年生去叶鞘针叶（p〈0.05）,其余元素在当年生和一年生的去叶鞘针叶与带叶鞘的完整针叶间均无显著差异;②Ni在当年生针叶及其叶鞘间没有显著差异（p〈0.05）,其它被测定的金属元素无论在当年生还是一年生针叶中,均表现为叶鞘中的元素质量分数均显著高于去叶鞘针叶（p〈0.05）,体现了针叶与叶鞘在形态结构上的差异引起重金属积累的差异;而非金属元素N质量分数则针叶显著高于叶鞘;③一年生针叶中Ni质量分数显著低于当年生针叶,但Al、Pb、Zn质量分数显著高于当年生针叶,一年生叶鞘中所有金属元素的质量分数都显著高于当年生叶鞘,表现出随暴露时间和针叶寿命的延长而持续累积的特征;而营养元素N质量分数在当年生针叶和一年生针叶以及当年生叶鞘和一年生叶鞘间无显著差异;④城乡环境梯度下生长的马尾松为研究环境质量演变提供了一个天然的实验室,受人类活动干扰大的城区马尾松一年生针叶的Cd和N质量分数及其叶鞘中的Cu、Cr、Ni质量分数显著高于受人为干扰较少的郊区,表明植物组织中元素化学特征反映了城市发展过程中人类活动的足迹。在城市环境尤其是重金属污染的监测中,成熟的一年生叶鞘更具指示意义,效果更显著。     

甜笋竹引种栽培丰产技术研究

甜竹笋味美甘甜，营养丰富，是上乘的森林蔬菜，市场前景广阔。1993年开始规模化引种栽培与丰产技术研究，结果表明：①甜笋竹适合赣南生长和种植；②掌握了甜竹笋的丰产技术；③种植3a后每hm^2产鲜笋17250kg以上。     

年产1.5万m~3中纤板生产线的扩产改造

简要介绍了年产 1.5万 m3中纤板生产线改造前的情况 ,重点论述了关键设备和工艺的改造过程 ,以及改造后的情况。结果表明 :改造后生产线的生产能力可以达到 3万 m3/ a的预期目标     

Oxidative stress-activated JNK-MAPK signaling pathway is involved in fluctuant high glucose-induced injury of hepatocytes

AIM: To explore the mechanisms of fluctuant high blood glucose-induced apoptosis of hepatocytes. METHODS: SD rats were randomly divided into normal control group (N), stable high blood glucose group (S), fluctuant high blood glucose group (F) and insulin group (I). Diabetic rats were induced by intraperitoneal injection of streptozotocin (65 mg/kg), and the fluctuant high blood glucose animal model was induced by intraperitoneal injection of ordinary insulin and glucose at different time points every day. The blood glucose fluctuation patterns of the animals in F group within 12 weeks were similar every day and no significant difference of the HbA1c concentration was observed compared with S group, indicating that the fluctuant hyperglycemia was successfully established in F group. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the content of malondialdehyde (MDA) and nitric oxide (NO) in the homogenate of the liver tissues were detected by colorimetry. The mRNA and protein levels of JNK, p-JNK, Bax and Bcl-2 were examined by RT-PCR and Western blot. RESULTS: After 12 weeks, the increases in the intakes of food and water, the urine output, and the abnormal liver function were observed in S group, I group and F group. Compared with N group, the MDA level was increased, the content of NO and the activity of SOD and GSH-Px were decreased, and up-regulation of JNK mRNA and p-JNK and Bax proteins, and down-regulation of Bcl-2 were also found in S group, I group and F group. The above effects were more obviously showed in F group. CONCLUSION: Oxidative stress activates JNK-MAPK signaling pathway, which is involved in fluctuant high glucose-induced apoptosis of hepatocytes.     

Cx43 is involved in electrical remodeling of atrial myocytes through regulating L-type calcium current

AIM: To investigate whether the association of connexin 43(Cx43) and L-type calcium channel involved in the pathogenesis of atrial fibrillation (AF). METHODS: The biochemical assays and whole-cell patch-clamp technique were used to study the expression of Cx43 in human atrial tissue. The co-localization of Cx43 and L -type calcium channel, and the regulation of L-type calcium current in atrial myocytes were investigated. RESULTS: The expression of Cx43 at mRNA and protein levels was decreased in human atrial tissues of AF patients. In cultured atrium-derived myocytes (HL-1 cells), knockdown of Cx43 significantly inhibited the mRNA expression of L-type calcium channel α1c subunit, as well as L-type calcium current. Co-localization of Cx43 with L-type calcium channel α1c subunit in mouse atrial myocytes was observed. CONCLUSION: The decrease in Cx43 is involved in the pathogenesis of AF, probably through reducing the L-type calcium current in atrial myoctyes by co-localization with L-type calcium channel, thus representing the potential pathogenesis in atrial fibrillation.     

冬小麦杂种F_1及其亲本光合特性的研究初报

研究了 10个冬小麦 (Triticum aestivum L.)杂交组合的子一代 (F1)及其亲本的光合特性。结果表明 ,在这 10个杂交组合中 ,虽然只各有 4个杂交组合的 F1的 PS 总的光化学量子产额 (Yield)和光化学荧光猝灭系数 (q P)有超亲现象 ,但绝大多数组合的 F1光合功能都与母本十分相近 ,正反交组合的结果进一步证明这种相关性。可见 ,母系遗传对于 F1光合功能的优劣有着十分重要的作用。根据所得的实验结果 ,认为在配置冬小麦杂交组合时 ,选择具有优良光合功能的品种作母本 ,可为进一步筛选光合功能好的稳定品种创造条件 ,是加速小麦育种进程的重要途径之一     

小麦与不同作物间作模式对麦蚜及主要捕食性天敌群落的影响

应用群落生态学结构变化特征的研究方法,研究了小麦与油菜、荷兰豆间作和单作麦田3种栽培模式条件下对麦田麦蚜及主要天敌群落的影响。结果表明,捕食性天敌的丰盛度和昆虫群落的稳定性,麦套荷兰豆最高,其次为麦套油菜田、单作麦田最低;麦套荷兰豆田麦蚜亚群落稳定性较好,单作麦田捕食性天敌亚群落的稳定性较好。麦蚜亚群落主要特征值分析表明,多样性指数和均匀度为:麦套荷兰豆>单作麦田>麦套油菜,优势度和优势集中性指数为:麦套油菜>单作麦田>麦套荷兰豆;主要捕食性天敌亚群落的多样性指数和均匀度为:单作麦田>麦套油菜>麦套荷兰豆,优势度和优势集中性指数为:麦套荷兰豆田>麦套油菜田>单作麦田。