柳叶栎不同种源生长变异研究

从柳叶栎原产地美国宾夕法尼亚、田纳西、阿肯色和路易斯安娜等4个州引进9个柳叶栎种源,于1999年分别在江苏东海县、宜兴市等地进行造林试验,对试验林进行了8 a观测,调查了造林当年成活率和3 a时保存率,测定了树高、地径、幅冠等生长指标。结果表明,9个不同种源间生长有显著差异,其树高、地径和冠幅的遗传变异系数分别为8.26%~11.42%,14.14%~18.9%和5.9%~13.6%。1~8 a时,柳叶栎种源的树高和地径年际间相关显著,具有早期选择潜力。按综合指数评定法,初选出9818号和9817号2个综合性状表现好的优良种源,其树高和地径比参试种源平均值增加10.6%和13.9%。     

套袋黄冠梨果面褐斑病发生的气象因子分析

本文根据近些年来套袋黄冠梨果面褐斑病发生情况，结合相关气象因子资料，综合分析得出，套袋黄冠梨果面褐斑病的发生与高湿低温有密切关系，降雨是诱导发病的关键气象因子：     

粘粒含量——夏季风的良好替代指标

通过对位于东亚季风区尾闾部位的沙沟剖面、东部半湿润区王官剖面、东部湿润区扣马剖面的气候代用指标的分析发现 ,黄土地层中 <5 μm的粘粒含量指标对各剖面中黄土-古土壤序列指示得比较清晰 ,可以较好地反映东亚夏季风的变化。对黄土地层中粘粒含量的测试及其环境意义分析以及与经典的东亚夏季风的替代指标———磁化率、红度指标 (a )的对比分析发现 ,粘粒含量指标的意义比较明确 ,适应范围更广 ,灵敏性也较高 ,是一比较可靠的、新的东亚夏季风的良好替代指标。     

厚层基材喷播技术在北方半干旱区岩石边坡植被恢复中的应用——以京承高速公路(三期)植被恢复工程为例

以京承高速公路(三期)岩石边坡植被恢复工程为例,研究厚层基材喷播技术的施工工艺及植被恢复效果,从抗旱乡土植物的筛选与配置、技术适宜性评价以及养护措施效果方面,综合分析归纳出厚层基材喷播技术,在北方半干旱地区岩石边坡植被恢复中的应用效果及关键技术,对于完善我国裸露边坡植被恢复理论体系,指导裸露坡面工程绿化,都具有非常重要价值。     

绿色建筑

绿色建筑（Sustainable or green building）是建筑设计和施工的最新的,并将持久地影响本星球未来的趋势。绿色建筑的概念,包括促进节能、环境友好和可持续发展的建筑设计、施工和维修。绿色建筑选用适当的地点,利用绿色建材和最佳照明,并安装节能的设备。现代产品的复杂性使衡量其可持续性相当困难。     

类猪圆环病毒因子P1 VP2蛋白二级结构与B细胞表位预测

旨为预测类猪圆环病毒因子P1 VP2蛋白的B细胞表位.采用生物信息软件和互联网服务器,预测VP2的二级结构,分析VP2表面特性(亲水性、可塑性、可及性和抗原性),综合预测VP2的B细胞抗原表位.结果表明,P1 VP2蛋白肽链的6-18和80-92区段为预测的B细胞表位优势区.多参数方案综合预测P1 VP2蛋白的B细胞抗原表位,为进一步鉴定表位及设计疫苗奠定了基础.     

植物无糖组培快繁装置及其环境控制系统的研制

针对常规植物组培存在的问题,从植物无糖组培实用化角度出发,研制了带有新型CO2施放装置的180 L植物无糖组培容器及其环境控制系统,采用小流量控制、三通阀调节和PWM控制方式,实现了对CO2浓度的精确控制,控制精度达到±50 μmol·mol^-1;采用穴盘覆膜与气体循环吸附相结合的方式实现了对容器内相对湿度的自动控制,控制精度达到±22%.通过圆叶海棠的无糖组培的试验,结果表明,该系统对组培苗的生长环境和生理品质的提高具有显著的促进作用.     

Prokaryotic Expression of Brucella BAB Gene and Establishment of iELISA

In this study,the genome of Brucella Rev.1 strain was used as a template to amplify the BAB gene sequence,clone it into the prokaryotic expression vector pET-30a(+),obtain the recombinant plasmid pET-30a-BAB,and perform prokaryotic expression on the recombinant plasmid.The indirect ELISA method was constructed by using the expression products detected by Western blotting.The results showed that the BAB gene was successfully cloned and expressed in this experiment,and the purified expression product was analyzed by SDS-PAGE.This study obtained a relatively pure recombinant BAB protein;Western blotting test showed that the expressed protein could react specifically with Brucella sheep positive serum and had good reactogenicity;Using the recombinant BAB protein as the coating antigen,an indirect ELISA method for detecting BAB antibodies was established and optimized.The best determined coating conditions were as follows BAB protein coating amount was 0.25 μg/mL,serum dilution was 1:400;blocking solution was 3% pig-derived gelatin;secondary antibody dilution was 1:6 000;color development time was 10 min.The established method was used to detect 40 clinical sheep serums,and the cut-off value was calculated to be 0.607.That was,when the serum tested had P/N ≥ 1.5 and D_{450 nm} ≥ 0.607,it was judged as positive,when D_{450 nm} ≤ 0.561,it was judged as negative,and when 0.607<D_{450 nm}<0.561,it was judged as a suspect value,and retest was required.Compared with the Huhong plate test and the test tube agglutination test,the positive coincidence rate was 100%,the negative coincidence rate was 71.88%,and the total coincidence rate was 77.5%.