全文获取类型
收费全文 | 19812篇 |
免费 | 1303篇 |
国内免费 | 2106篇 |
专业分类
林业 | 1389篇 |
农学 | 1211篇 |
基础科学 | 926篇 |
2255篇 | |
综合类 | 8970篇 |
农作物 | 1360篇 |
水产渔业 | 898篇 |
畜牧兽医 | 3866篇 |
园艺 | 1391篇 |
植物保护 | 955篇 |
出版年
2024年 | 127篇 |
2023年 | 384篇 |
2022年 | 940篇 |
2021年 | 987篇 |
2020年 | 913篇 |
2019年 | 858篇 |
2018年 | 626篇 |
2017年 | 999篇 |
2016年 | 675篇 |
2015年 | 1006篇 |
2014年 | 1067篇 |
2013年 | 1308篇 |
2012年 | 1707篇 |
2011年 | 1710篇 |
2010年 | 1709篇 |
2009年 | 1445篇 |
2008年 | 1485篇 |
2007年 | 1289篇 |
2006年 | 990篇 |
2005年 | 862篇 |
2004年 | 525篇 |
2003年 | 387篇 |
2002年 | 305篇 |
2001年 | 299篇 |
2000年 | 268篇 |
1999年 | 96篇 |
1998年 | 25篇 |
1997年 | 24篇 |
1996年 | 25篇 |
1995年 | 11篇 |
1994年 | 11篇 |
1993年 | 21篇 |
1992年 | 21篇 |
1991年 | 17篇 |
1990年 | 13篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 14篇 |
1986年 | 7篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1978年 | 3篇 |
1974年 | 3篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1964年 | 2篇 |
1963年 | 2篇 |
1962年 | 4篇 |
1956年 | 8篇 |
1955年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
42.
ITS—RFLP分析进行昆虫病原线虫斯氏属和异小杆属的分类鉴定 总被引:2,自引:1,他引:2
本研究通过PCR扩增和六种限制性内切酶(AluⅠ,HinfⅠ,MboⅠ,RsaⅠ,HaeⅢ和PvuⅡ)酶切,对国内害虫防治上常用的几种昆虫病原线虫,包括斯氏属S.car-pocapsae,S.feltiae和S.glaseri以及异小杆属H.bacteriophora,H.zealandica,H.indicus和H.megidis等8个品系rDNA-ITS进行分析。建立起可以区分各线虫种的标准RFLP图谱。该方法快速简便,稳定可靠,需要的样品量少。可以用于新鲜的,或冻存的样品,甚至分析单条的线虫,不仅可进行昆虫病原线虫的快速分类鉴定。而且进一步可以应用于线虫田间释放的辅助监测。实际田间感染率的测定和线虫毒力的比较。 相似文献
43.
对山西菜田捕食性食蚜蝇优势种及对菜蚜的控制效果进行了研究,菜田常见捕食性食蚜蝇有黑带食蚜蝇,大灰食蚜蝇,斜斑鼓额食蚜蝇,野食蚜蝇,四条小食蚜蝇,短翅细腹食蚜蝇6种,以黑带食蚜蝇种群数量最大,平均占全年发生总量的36.3%,通过对食蚜蝇产卵量,卵化率,幼虫期捕食量和羽化率测试,筛选出的黑带食蚜蝇,大灰食蚜蝇,斜斑鼓额食蚜蝇幼虫对菜蚜均有较强的捕食能力,其中黑带食蚜蝇为山西菜田菜蚜捕食性食蚜蝇优势种,用黑带食蚜蝇三龄幼虫对菜蚜进行防治试验,结果以1:180防效最佳,72h后控制效果达93.5%。 相似文献
44.
45.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P<0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P<0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P<0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P<0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P<0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation. 相似文献
46.
AIM: To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes(SCDC) of mouse. METHODS: Embryonic stem cells of D3 line(ES-D3) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D3 cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC. RESULTS: The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC. CONCLUSION: The functional expression of ion channels occurred during ES-D3 cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC. 相似文献
47.
48.
49.
D. Boscia H. Zeramdini M. Cambra O. Potere M.T. Gorris A. Myrta B. Di Terlizzi V. Savino 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(5):477-480
A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date. 相似文献
50.