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41.
42.
Charmantier A McCleery RH Cole LR Perrins C Kruuk LE Sheldon BC 《Science (New York, N.Y.)》2008,320(5877):800-803
Rapid climate change has been implicated as a cause of evolution in poorly adapted populations. However, phenotypic plasticity provides the potential for organisms to respond rapidly and effectively to environmental change. Using a 47-year population study of the great tit (Parus major) in the United Kingdom, we show that individual adjustment of behavior in response to the environment has enabled the population to track a rapidly changing environment very closely. Individuals were markedly invariant in their response to environmental variation, suggesting that the current response may be fixed in this population. Phenotypic plasticity can thus play a central role in tracking environmental change; understanding the limits of plasticity is an important goal for future research. 相似文献
43.
Identification of the cystic fibrosis gene: chromosome walking and jumping 总被引:253,自引:0,他引:253
J M Rommens M C Iannuzzi B Kerem M L Drumm G Melmer M Dean R Rozmahel J L Cole D Kennedy N Hidaka 《Science (New York, N.Y.)》1989,245(4922):1059-1065
An understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA. 相似文献
44.
Construction of a general human chromosome jumping library, with application to cystic fibrosis 总被引:22,自引:0,他引:22
F S Collins M L Drumm J L Cole W K Lockwood G F Vande Woude M C Iannuzzi 《Science (New York, N.Y.)》1987,235(4792):1046-1049
In many genetic disorders, the responsible gene and its protein product are unknown. The technique known as "reverse genetics," in which chromosomal map positions and genetically linked DNA markers are used to identify and clone such genes, is complicated by the fact that the molecular distances from the closest DNA markers to the gene itself are often too large to traverse by standard cloning techniques. To address this situation, a general human chromosome jumping library was constructed that allows the cloning of DNA sequences approximately 100 kilobases away from any starting point in genomic DNA. As an illustration of its usefulness, this library was searched for a jumping clone, starting at the met oncogene, which is a marker tightly linked to the cystic fibrosis gene that is located on human chromosome 7. Mapping of the new genomic fragment by pulsed field gel electrophoresis confirmed that it resides on chromosome 7 within 240 kilobases downstream of the met gene. The use of chromosome jumping should now be applicable to any genetic locus for which a closely linked DNA marker is available. 相似文献
45.
Radiation carcinogenesis: the sequence of events 总被引:4,自引:0,他引:4
46.
Focal antibody production by transferred spleen cells in irradiated mice 总被引:18,自引:0,他引:18
Lethally irradiated mice were injected with small numbers of normal spleen cells and then immunized with sheep erythrocytes. Antibody activity was found in their spleens in localized areas whose number corresponded to the number of spleen cells injected. When sheep and pig erythrocytes were injected together, antibody against each was found in separate areas. Each area may consist of the progeny of a single precursor cell, restricted to forming a single antibody. 相似文献
47.
Sex chromosomes in lizards 总被引:2,自引:0,他引:2
Karyotypes of many species of the genus Sceloporus support the generalization that there are no morphologically recognizable sex chromosomes in lizards; however, there is a marked sexual dimorphism in the karyotypes of Sceloporus jarrovi and Sceloporus poinsetti. During meiosis in males, whose diploid number of chromosomes is 31, preferential segregation of chromosomes from a trivalent results in heterogamety. 相似文献
48.
Diphtheria toxin subunit active in vitro 总被引:7,自引:0,他引:7
Exposure of diphtheria toxin to dithiothreitol (and similar thiols) resulted in a subunit which was active in catalyzing the adenosine diphosphateribosylation of mammalian aminoacyl-transferase II in the presence of nicotinamide adenine dinucleotide. At the same time there was a marked increase in total ADP-ribosylation activity. A molecule which was apparently identical to the derived subunit in size and activity was detected in partially purified preparations of toxin. 相似文献
49.
Cole AD 《Science (New York, N.Y.)》1893,21(540):316-317
50.