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Tomokazu OHTA Katsuo KOSHI Koichi USHIZAWA Misa HOSOE Toru TAKAHASHI Takahiro YAMAGUCHI Keiichiro KIZAKI Kazuyoshi HASHIZUME 《The Journal of reproduction and development》2013,59(6):507-511
T cells are the dominant lymphocytes in the endometrium and are considered to play a
crucial role in implantation and in the maintenance of gestation through cytokine
production and immune regulation. The mechanisms underlying immunoregulation at the
feto-maternal interface are still obscure for this complex system. Understanding the
role of T cells is a key factor in understanding the endometrial immune system. In
this study, the distribution of endometrial CD3+ T cells in bovines was
examined by immunohistochemical analysis. The estrous cycle and gestation was divided
into 4 stages, and the number of CD3+-positive T cells was counted in each
stage. CD3+ cells were found in the endometrium in significant numbers
throughout the estrous cycle and were mostly located in the subepithelial area. The
number of CD3+ cells significantly increased in the early and mid-luteal
phases but decreased after implantation with the progression of gestation. No T cells
were found in the placentome or specifically in the tissues near the fetus, including
the trophoblastic area. In addition, very few T cells were found in stromal regions
close to the myometrium of the endometrium. These findings suggest that
downregulation of bovine endometrial CD3+ T-cell functions is closely
related to the successful maintenance of gestation in a spatiotemporal manner. 相似文献
53.
Satoshi Shimamura Takeshi Nishimura Tomokazu Koshiba Ryo Yamamoto Susumu Hiraga Takuji Nakamura 《Plant Production Science》2016,19(1):154-160
In flooded hypocotyl of soybean (Glycine max), cell division in phellogen and the elongation of these cells are enhanced, and thereby a secondary aerenchyma with high porosity is produced. Auxin controls cell division and cell elongation in many plants, so we studied its role in secondary aerenchyma development. Soybean plants with fully expanded unifoliolate leaves were flooded for 6 d with solutions (100 μM each) of seven anti-auxins. TIBA, NPA, HFCA, 1-NOA, or CHPAA did not restrict the secondary aerenchyma formation, while MH moderately suppressed the aerenchyma development, and PCIB strongly inhibited the development of phellogen and secondary aerenchyma. However, the endogenous IAA concentrations in the flooded hypocotyls did not increase or decrease relative to the controls until 72 h, when a secondary aerenchyma was observed. From these results, it is unclear whether auxin plays an important role in the process of secondary aerenchyma formation under flooding. 相似文献
54.
Tatsuyuki YOSHIDA Taro SATO Koji CHIBA Itaru YOSHIMURA Masahiro OSADA Konosuke OKADA Hiroki FURUTA Akihiko OHTA Hitoshi USHIJIMA 《Animal Science Journal》2012,83(7):529-534
This study was designed to evaluate the effect of estrus synchronization treatments on recipient heifers for embryo transfer (ET). Holstein heifers were separated into the following three groups: (i) an administration of 50 µg GnRH (gonadotropin‐releasing hormone) analog was given to heifers at a random stage of the estrus cycle, followed 7 days later by two administrations of 7.5 mg prostaglandin F2 alfa analog (PG) as control; (ii) another administration of 100 µg GnRH was given to the control group at 48 h after the administration of PG as the second GnRH group; and (iii) an administration of 0.75 mg estradiol benzoate (E2) was given to the control group at 24 h after the administration of PG as the E2 group. Each method caused estrus synchronization. Fresh embryos were nonsurgically transferred into the suitable recipients that had a functional corpora lutea (CL) 7 days after estrus. The E2 group showed a significantly higher (P < 0.01) rate of estrus synchronization (98.9%) at 1–3 days after PG administration and the final pregnancy rate of the E2 group (50.6%) was also significantly higher than the other groups (37.1%, P < 0.05 and 30.9%, P < 0.01, respectively). These findings demonstrate that E2 administration 24 h after PG protocol is effective for estrus synchronization of Holstein heifers, thus improving the productivity of ET. 相似文献
55.
Attempts were made to obtain high-resolution images of an unbeaten bleached softwood kraft pulp fiber surface in water by
applying contact mode atomic force microscopy. However, clear topographic images could not be obtained. In order to investigate
the possibility of deformation of a pulp fiber surface during scanning, force curve measurements were applied to pulp fiber
surfaces. It was found that a pulp fiber in water had a more deformable surface than an air-dried pulp fiber in air. Moreover,
the spring constant of it was estimated to be close to that of a cantilever applied for imaging. Therefore, the images of
a pulp fiber surface in water were thought to be significantly affected by deformation, which was considered to be an important
cause of the unclear images.
Parts of this article was presented at the 53rd Annual Meeting of the Japan Wood Research Society, Fukuoka, Japan, March 2003,
the 54th Annual Meeting of the Japan Wood Research Society, Hokkaido, Japan, August 2004, the 12th International Symposium
on Wood and Pulping Chemistry, Madison, USA, June 2003, and the 13th International Symposium on Wood, Fiber and Pulping Chemistry,
Oakland, New Zealand, May 2005 相似文献
56.
Shoma MIKAWA Yasuhiro OHTA Noriyuki KAJI Md Shafiqul ISLAM Takahisa MURATA Hiroshi OZAKI Masatoshi HORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1443-1449
Endotoxin causes gastrointestinal motility disorder. Aim of this study is to clarify
inhibitory mechanisms of lipopolysaccharide (LPS) on smooth muscle contraction in rat
ileum. Ileal tissues were isolated from control rat or from LPS-induced peritonitis model
rat. Treatment with LPS inhibited carbachol (CCh)-mediated contraction in a time-dependent
manner. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) genes were
also upregulated, but iNOS expression was preceded by a rising of COX-2. All subtypes of
prostaglandin E2 (PGE2) receptors (EP1-EP4) were expressed in ileum,
and PGE2 and selective EP2 or EP4 agonist inhibited CCh-mediated contraction.
Selective iNOS inhibitor did not reverse LPS-induced inhibition of contraction by CCh at 1
and 2 hr, but reduced the inhibitory action at 4 hr after the LPS treatment. COX-2
inhibitor reversed the inhibitory action by LPS in all exposure time. Finally, in ileal
tissues isolated from peritonitis model rat, iNOS expression was upregulated only at 4 hr
after LPS administration, resulting in enhanced inhibitory action of LPS against
CCh-induced contraction. In conclusion, LPS induces COX-2 to produce PGE2,
which initially activates EP2 and/or EP4 on smooth muscle cells to inhibit the
contractility in early phase of LPS exposure. Moreover, in late phase of LPS treatment,
iNOS is expressed to produce NO, which in turn inhibited the contraction by CCh. The
inhibitory cascade is similar in the ileum isolated from peritonitis model rat, indicating
time-dependent changes of inhibitory action by LPS on intestinal motility in
peritonitis. 相似文献
57.
Takashi KOZASA Yuri ABE Kazuya MITSUHASHI Tomokazu TAMURA Hiroshi AOKI Masatoshi ISHIMARU Shigeyuki NAKAMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518
The Exaltation of Newcastle disease virus (END) phenomenon is induced by the
inhibition of type I interferon in pestivirus-infected cells in vitro,
via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the
property of the viral autoprotease protein Npro. Reportedly, the amino acid
residues in the zinc-binding TRASH motif of Npro determine the difference in
characteristics between END-phenomenon-positive (END+) and
END-phenomenon-negative (END−) classical swine fever viruses (CSFVs). However,
the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not
been elucidated from the genomic differences between END+ and END−
viruses using reverse genetics till date. In the present study, comparison of complete
genome sequences of a pair of END+ and END− viruses isolated from
the same virus stock revealed that there were only four amino acid substitutions (D136G,
I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with
and without mutations at these positions were generated using reverse genetics. The END
assay, measurements of induced type I interferon and IRF-3 detection in cells infected
with these viruses revealed that the aspartic acid at position 136 in the zinc-binding
TRASH motif of Npro was required to inhibit the production of type I interferon
via the degradation of cellular IRF-3, consistently with CSFV. 相似文献
58.
Rommaneeya LEELA-ARPORN Hiroshi OHTA Genya SHIMBO Noboru SASAKI Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1459
The size of canine focal liver lesions (FLLs) is known to be one of the predicting criteria for malignancy. However, there are discrepancies for the measurement of maximum lesion size, resulting in contradicting results among studies and incidences of false positive outcomes. Thus far, the morphometric changes of FLLs for distinguishing malignancy from benignancy remains undocumented. This study aimed to investigate morphometric characteristics of FLLs using computed tomography (CT). CT images of 40 dogs with histopathological confirmation of 49 liver lesions, including 39 hepatocellular carcinomas and 10 nodular hyperplasias were retrospectively reviewed. The morphometric parameters including size (long and short axis diameters measured on transverse image), shape (measured by long to short axis (L/S) ratio), volume, and surface appearance of a liver lesion were evaluated using univariate and stepwise multivariate analyses, respectively. The results of univariate analysis showed that long and short axis diameters, L/S ratio, volume, and surface appearance of a lesion were significantly different between hepatocellular carcinomas and nodular hyperplasias. Multivariate analysis revealed that short axis diameter (>3.30 cm; odds ratio (OR): 36.1, 95% confidence interval (CI): 3.36–387.05, P=0.0031) and L/S ratio (>1.23; OR: 18.1, 95% CI: 1.61–205.12, P=0.0191) were independent predictors of malignancy, with the area under the curve of 0.9154. These results suggest that the combination of short axis diameter and L/S ratio is a promising tool for predicting liver malignancy with outstanding discriminating ability. 相似文献
59.
Saiko Sugawara Toshihiko Ito Sho Sato Mari Yokoo Yuki Mori Kano Kasuga Ikuo Kojima Tomokazu Fukuda Ken‐ichi Yamanaka Miki Sakatani Masashi Takahashi Masayuki Kobayashi 《Animal Science Journal》2013,84(3):275-280
Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the proper development of bovine embryos. We recently determined the FGF4 gene sequence in eight cattle derived from three breeds and revealed a common nucleotide sequence of the structural gene encoding FGF4, which leads to the deletion and mutation of amino acid sequences in the mature FGF4 (Pro32‐Leu206) compared with the sequence previously reported. In the present study, HisbFGF4, a 6× histidine‐tagged bovine FGF4 (Pro32‐Leu206), was produced in Escherichia coli based on the validated nucleotide sequence and purified by heparin column chromatography. In primary bovine fibroblasts, HisbFGF4 showed significant mitogenic activity, whereas, intriguingly, the activity of a commercially available recombinant human FGF4 (Gly25‐Leu206) produced in E. coli was weaker than that of HisbFGF4. In conclusion, the present study provides a simple method for the production of a bioactive bovine FGF4 derivative in E. coli utilizing its structural gene elucidated by us. 相似文献
60.
Yuhua Bai Ming Zhao Liming Bai Ryo Hasegawa Jun-ichi Sakai Toshiaki Hasegawa Tomokazu Mitsui Hirotsugu Ogura Takao Kataoka Katsutoshi Hirose Masayoshi Ando 《Journal of Wood Science》2011,57(1):56-65
Sixteen cardenolide triglycosides (1?C16) were isolated from stems, twigs, and leaves of Nerium oleander. Among them, 3??-O-(4-O-gentiobiosyl-d-diginosyl)-7??,8-epoxy-14-hydroxy-5??,14??-card-20(22)-enolide, named cardenolide B-3 (16), was isolated from natural sources for the first time. The in vitro anti-inflammatory activities of compounds 1?C16 were examined on the basis of inhibitory activity against the induction of intercellular adhesion molecule-1 (ICAM-1). Compounds 1?C5 were active at an IC50 value of less than 7 ??M. The cytotoxic activity of isolated compounds was evaluated against three human cell lines: normal human fibroblast cells (W-38), malignant tumor cells induced from WI-38 (VA-13), and human liver tumor cells (HepG2). Compounds 1?C5 were active toward WI-38 cells; compounds 1, 3, and 5 were active toward VA-13 cells; and compounds 1?C5 were active toward HepG2 cells at IC50 values of less than 10 ??M. The multidrug-resistant (MDR) cancer-reversal activity of compounds 1?C16 was evaluated on the basis of the amount of calcein accumulated in MDR human ovarian cancer 2780AD cells in the presence of each compound. Compounds 13 and 14 showed significant effects on calcein accumulation. 相似文献