Influence of maternal immunity on vaccine efficacy and susceptibility of one day old chicks against Egyptian highly pathogenic avian influenza H5N1

In Egypt, continuous circulation of highly pathogenic avian influenza (HPAI) H5N1 viruses of clade 2.2.1 in vaccinated commercial poultry challenges strenuous control efforts. Here, vaccine-derived maternal AIV H5 specific immunity in one-day old chicks was investigated as a factor of vaccine failure in long-term blanket vaccination campaigns in broiler chickens. H5 seropositive one-day old chicks were derived from breeders repeatedly immunized with a commercial inactivated vaccine based on the Potsdam/H5N2 strain. When challenged using the antigenically related HPAIV strain Italy/98 (H5N2) clinical protection was achieved until at least 10 days post-hatch although virus replication was not fully suppressed. No protection at all was observed against the Egyptian HPAIV strain EGYvar/H5N1 representing a vaccine escape lineage. Other groups of chicks with maternal immunity were vaccinated once at 3 or 14 days of age using either the Potsdam/H5N2 vaccine or a vaccine based on EGYvar/H5N1. At day 35 of age these chicks were challenged with the Egyptian HPAIV strain EGYcls/H5N1 which co-circulates with EGYvar/H5N1 but does not represent an antigenic drift variant. The Potsdam/H5N2 vaccinated groups were not protected against EGYcls/H5N1 infection while, in contrast, the EGYvar/H5N1 vaccinated chicks withstand challenge with EGYvar/H5N1 infection. In addition, the results showed that maternal antibodies could interfere with the immune response when a homologous vaccine strain was used.     

Temporal changes of soil physical and hydraulic properties in strawberry fields

Even over short time intervals, soil properties are subject to variation, especially in managed soils. The objective of this study was to assess the temporal changes of soil physical and hydraulic properties in strawberry fields cultivated under surface drip fertigation in Turuçu, Brazil. Intact core samples were collected from the near surface soil layer of seedbeds to determine the total porosity (TP), macroporosity (MA), matrix porosity, bulk density (BD), available water capacity (AWC), field capacity, wilting point and Dexter’s S index. Aggregate samples were collected from the arable layer to determine the aggregate size distribution and aggregate mean weight diameter. All samples were collected from 15 strawberry fields and at four different times during the 2007–2008 strawberry growing cycle. Although soil pore‐solid relations are expected to adjust soon after seedbed construction, their variation was only evident after >13 weeks. Even though values of TP and MA decreased with time, and those of BD increased near the end of the growing cycle, all the soils maintained their capacity to support root activity as indicated by critical values of Dexter’s index (S > 0.03). The amount of relatively large aggregates (9.51–2.00 mm) and AWC increased towards the end of the strawberry cultivation cycle. With changes in soil structure improving soil physical quality, strawberry development benefitted. We showed that if farmers gradually increase the amount of water through fertigation to a maximum value occurring at the end of crop cycle instead of applying water at a constant rate, water and energy use efficiency in agriculture would improve.     

Significance of the fructose/glucose ratio in screening potato breeding lines with processing potential

Low sugar accumulation during low temperature holding is a most sought after characteristic in a potato cultivar utilized for processing. Although this property is largely inherited, the lack of a reliable biochemical test capable of screening large numbers of new breeding lines with low sugar accumulating ability has hampered present selection procedures of plant breeders. This study examined the concentration of individual sugar components and the ratios of sugar components from 80 to 110 day-old plants of three commercial potato cultivars, White Rose, Russet Burbank and Norchip, each with different inherent capacity to accumulate sugars under low temperature holding. By determining the fructose and glucose concentration of potatoes from 110-day old plants and calculating the fructose/ glucose ratio, it was possible at harvest to separate cultivars that accumulate high concentrations of sugars during cold storage from those that accumulate low concentration of sugars     

A Coralline Algal-Associated Bacterium,Pseudoalteromonas Strain J010, Yields Five New Korormicins and a Bromopyrrole

The ethanol extract of Pseudoalteromonas strain J010, isolated from the surface of the crustose coralline alga Neogoniolithon fosliei, yielded thirteen natural products. These included a new bromopyrrole, 4′-((3,4,5-tribromo-1H-pyrrol-2-yl)methyl)phenol (1) and five new korormicins G–K (2–6). Also isolated was the known inducer of coral larval metamorphosis, tetrabromopyrrole (TBP), five known korormicins (A–E, previously named 1, 1a–c and 3) and bromoalterochromide A (BAC-A). Structures of the new compounds were elucidated through interpretation of spectra obtained after extensive NMR and MS investigations and comparison with literature values. The antibacterial, antifungal and antiprotozoal potential of 1–6, TBP and BAC-A was assessed. Compounds 1–6 showed antibacterial activity while BAC-A exhibited antiprotozoal properties against Tetrahymena pyriformis. TBP was found to have broad-spectrum activity against all bacteria, the protozoan and the fungus Candida albicans.     

Potato composition: III. Tissue selection and its effects on total nitrogen,free amino acid nitrogen and enzyme activity (polyphenolase,monophenolase, peroxidase and catalase)

Russet Burbank, Norchip, Kennebec, White Rose, Red La Soda and Lenape tubers were sampled at harvest, after storage for 2 and 4 mo at 7 C, and after 3 wk at 20 C following each storage treatment. Freeze-dried tissue from the bud-end, stem-end and core was analyzed for total, and free amino acid nitrogen, and for monophenolase, polyphenolase, peroxidase, and catalase activities. Significant differences among cultivars and among different parts of the tuber were found for all nitrogen fractions and all enzyme activities. Storage treatments significantly changed the total and free amino acid nitrogen contents and polyphenolase, peroxidase, and catalase activities. When compared for total nitrogen content and catalase activity, differences among different parts of the tuber which were statistically significant were similar in all cultivars. However, when compared for free amino acid content, and monophenolase, polyphenolase and peroxidase activities, differences among different parts of the tuber which were statistically significant were dependent upon which cultivar was examined. The differences among cultivars which were statistically significant for all nitrogen fractions and all enzyme activities, except peroxidase, were not significantly changed by storage treatment. The differences among different parts of the tuber which were statistically significant for quantity of both nitrogen fractions and for all enzyme activities were not significantly changed by storage.     

Bestimmungsschlüssel für Eier und Eigelege von Kriebelmückenarten (Dipt., Simuliidae) unter besonderer Berücksichtigung mammalophiler Schad- und Plageerreger

Biting species of Simuliidae are a pest to men and cattle. Black flies oviposite either directly into running water or on floating vegetation, but up to now oviposition is observed only in few species. In this paper the first key for determinating eggs and egg-masses of 14 Middle-European black fly species is given, especially for pest species (Boophthora erythrocephala, Odagmia ornata, Simulium morsitans, Wilhelmia equina, W. lineata). In addition, some details are reported about the egg-biology of included species.