全文获取类型
收费全文 | 141篇 |
免费 | 10篇 |
专业分类
林业 | 10篇 |
农学 | 2篇 |
基础科学 | 2篇 |
23篇 | |
综合类 | 24篇 |
农作物 | 2篇 |
水产渔业 | 10篇 |
畜牧兽医 | 69篇 |
园艺 | 1篇 |
植物保护 | 8篇 |
出版年
2023年 | 1篇 |
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 4篇 |
2014年 | 5篇 |
2013年 | 4篇 |
2012年 | 7篇 |
2011年 | 9篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 8篇 |
2007年 | 14篇 |
2006年 | 5篇 |
2005年 | 7篇 |
2004年 | 7篇 |
2003年 | 16篇 |
2002年 | 7篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有151条查询结果,搜索用时 31 毫秒
11.
Yu SW Wang H Poitras MF Coombs C Bowers WJ Federoff HJ Poirier GG Dawson TM Dawson VL 《Science (New York, N.Y.)》2002,297(5579):259-263
Poly(ADP-ribose) polymerase-1 (PARP-1) protects the genome by functioning in the DNA damage surveillance network. PARP-1 is also a mediator of cell death after ischemia-reperfusion injury, glutamate excitotoxicity, and various inflammatory processes. We show that PARP-1 activation is required for translocation of apoptosis-inducing factor (AIF) from the mitochondria to the nucleus and that AIF is necessary for PARP-1-dependent cell death. N-methyl-N'-nitro-N-nitrosoguanidine, H2O2, and N-methyl-d-aspartate induce AIF translocation and cell death, which is prevented by PARP inhibitors or genetic knockout of PARP-1, but is caspase independent. Microinjection of an antibody to AIF protects against PARP-1-dependent cytotoxicity. These data support a model in which PARP-1 activation signals AIF release from mitochondria, resulting in a caspase-independent pathway of programmed cell death. 相似文献
12.
Hong Li Robert J. Lascano Jill Booker L. Ted Wilson Kevin F. Bronson 《Soil & Tillage Research》2001,58(3-4):245-258
Landscape variability associated with topographic features affects the spatial pattern of soil water and N redistribution, and thus N uptake and crop yield. A landscape-scale study was conducted in a center pivot irrigated field on the southern High Plains of Texas in 1999 to assess soil water, soil NO3-N, cotton (Gossypium hirsutum L.) lint yield, and N uptake variability in the landscape, and to determine the spatial correlation between these landscape variables using a state-space approach. The treatments were irrigation at 50 and 75% cotton potential evapotranspiration (ET). Neutron access tubes were placed at a 15-m interval along a 710 m (50% ET) and 820 m (75% ET) transect across the field. Soil NO3-N in early spring was autocorrelated at a distance varying between 60 and 80 m. Measured soil volumetric water content (WC), total N uptake, and lint yield were generally higher on lower landscape positions. Cotton lint yield was significantly correlated to soil WC (r=0.76), soil NO3-N (r=0.35), and site elevation (r=−0.54). Differences of site elevation between local neighboring points explained the soil water, NO3-N and lint yield variability at the micro-scale level in the landscape. Soil WC, cotton lint yield, N uptake, and clay content were crosscorrelated with site elevation across a lag distance of ±30–40 m. The state-space analysis showed that cotton lint yield was positively weighted on soil WC availability and negatively weighted on site elevation. Cotton lint yield state-space models give insights on the association of soil physical and chemical properties, lint yield, and landscape processes, and have the potential to improve water and N management at the landscape-scale. 相似文献
13.
Wind erosion has degraded over one-half billion hectares of land worldwide. 137Cesium (137Cs) has been used as a tracer to study long-term rates of soil redistribution by water and, to a lesser extent, by wind. Early studies assumed that the decline in 137Cs activity for a potentially eroded soil relative to that for an uneroded soil was linearly proportional to soil loss. More recently, models have emerged that consider the effects of soil cultivation and the particle surface area-dependent partitioning of 137Cs on soils. We investigated the partitioning of 137Cs in wind-eroded sediments and with soil surface samples sieved into contiguous ranges of particle sizes. We also compared the 137Cs activities and stratification of several adjacent soils with known wind erosion and deposition histories. Finally, we tested 137Cs-based soil loss models with measured data from sites with documented histories. 137Cs activities and mean particle diameters of aeolian samples agreed well with the 137Cs activities and respective mean diameters of the sieved surface soil samples. Good agreement between model estimations and measured data indicated that 137Cs models developed to estimate soil redistribution by water were also applicable to soil redistribution by wind provided that the models contained an appropriate particle size correction parameter. 相似文献
14.
Bacterial translocation: clinical implications and prevention. 总被引:1,自引:0,他引:1
Douglass K Macintire Ted L Bellhorn 《Veterinary Clinics of North America: Small Animal Practice》2002,32(5):1165-1178
The occurrence of BT has been well documented in experimental animal models of hemorrhagic shock, trauma, severe burns, cirrhosis, pancreatitis, and bacterial overgrowth. Translocation of viable bacteria and endotoxins into mesenteric lymph nodes and other gut-associated lymphatic tissue is thought to activate a complex interplay of mediators that initiates the SIRS. Multiple humoral and cellular systems cause synthesis, expression, and release of inflammatory mediators, such as toxic oxygen radicals, proteolytic enzymes, adherence molecules, and various cytokines. A massive sustained proinflammatory response can ultimately result in irreversible multiple organ dysfunction. Because BT is associated with splanchnic hypoperfusion, the cornerstone of therapy involves rapid resuscitation and restoration of tissue perfusion. If a septic focus can be identified, it should be removed. Gut protectants, promotility agents, antioxidants, and immune-enhancing diets have shown promise in improving length of survival in these critically ill patients. 相似文献
15.
Ted C Schroeder Joseph Kovanda 《Veterinary Clinics of North America: Food Animal Practice》2003,19(2):397-417, vii
With their growth, it is important to consider how alliances will impact the beef industry in the future. Alliances have the potential to make sweeping changes to cattle production, live and feeder cattle marketing, food safety protocols, use of government grades and standards, ownership structure, supply chain management, wholesale and retail product marketing, risk management, and many other industry activities. In an effort to address these issues, this article addresses the following questions: What is an alliance? What has motivated their proliferation? What have we learned from alliances? What aspects of alliances affect their likelihood of success or failure? What is the future of alliances? Are they a fad or a long-term evolving industry structural change? 相似文献
16.
乳腺功能基因组学研究需要收集一些合适的cDNA序列来对各种亚细胞不同发育和运作阶段的基因表达形式进行评估。为了更好的捕捉基因表达的范围,我们用混合的牛乳腺细胞建立了1个标准化的cDNA文库,产生了23202个EST,存放的基因库中。这些在牛基因索引中带有序列的EST集形成当前23883个试验性分析序列中的5751个。5751个中的87%仅含有1-3个乳腺来源EST。相反,18%的乳腺EST有12个基因与TC序列相似,这些结果说明标准化文库仅有部分作用,因为EST中,能在泌乳过程中大量转录的基因的减少可能是由于合并引起的。为了更好的评价乳腺文库中的新内容,并把它们增加到现存的注释中(包括牛序列元件、基因一致性序列和人类基因组序列),我们对人和鼠基因诱变和在TOGA的基因非线性化进行测试,并把其结果增加到现有BtGI注释中。35000多个牛元件明显超过了人类基因组序列,而且一些与人类表达序列相关的排列(3%)的位置是独立的。由于3445TC序列与任何数据无明显相似性,代表这些元件中的23种乳腺源cDNA克隆被进一步进行分析用于表达和新异。其只驻有一个克隆符合标准,这相相应基因是一个分化的异端或表达对牛来说是特异的。结果说明,用作标志哺乳动物的转座轴和鉴定这些基因的牛序列表达数据仅针对反刍动物。 相似文献
17.
18.
Szatmári V Rothuizen J van den Ingh TS van Sluijs F Voorhout G 《Journal of the American Veterinary Medical Association》2004,224(5):717-727
OBJECTIVE: To determine ultrasonographic abnormalities in dogs with hyperammonemia. DESIGN: Retrospective study. ANIMALS: 90 client-owned dogs with hyperammonemia. PROCEDURE: Ultrasonography of the abdominal vessels and organs was performed in a systematic way. Dogs in which the ultrasonographic diagnosis was a congenital portosystemic shunt were included only if they underwent laparotomy or necropsy. Dogs in which the abdominal vasculature appeared normal and dogs in which the ultrasonographic diagnosis was acquired portosystemic shunts and portal hypertension were included only if liver biopsy specimens were submitted for histologic examination. RESULTS: Ultrasonography excluded portosystemic shunting in 11 dogs. Acquired portosystemic shunts were found in 17 dogs, of which 3 had arterioportal fistulae and 14 had other hepatic abnormalities. Congenital portosystemic shunts were found in 61 dogs, of which 19 had intrahepatic shunts and 42 had extrahepatic shunts. Intrahepatic shunts originated from the left portal branch in 14 dogs and the right portal branch in 5. Extrahepatic shunts originated from the splenic vein, the right gastric vein, or both and entered the caudal vena cava or the thorax. Ultrasonography revealed splenic-caval shunts in 24 dogs, right gastric-caval shunts in 9 dogs, splenic-azygos shunts in 8 dogs, and a right gastric-azygos shunt in 1 dog. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that ultrasonography is a reliable diagnostic method to noninvasively characterize the underlying disease in dogs with hyperammonemia. A dilated left testicular or ovarian vein was a reliable indicator of acquired portosystemic shunts. 相似文献
19.
20.