Compaction of forest soils with heavy logging machinery affects soil bacterial community structure

Soil compaction is widespread but tends to be most prevalent where heavy machinery is used in landfill sites, agriculture and forestry. Three forest sites strongly disturbed by heavy logging machinery were chosen to test the physical effects of different levels of compaction on soil bacterial community structure and soil functions. Community analysis comprised microbial biomass C and T-RFLP genetic profiling. Machine passes, irrespective of the compaction level, considerably modified soil structural characteristics at two soil depths (5–10 cm; 15–20 cm). Total porosity decreased up to 17% in the severe compaction. Reflected in this overall decline were large decreases in macroporosity (>50 μm). Reduction in macroporosity was associated with higher water retention and restricted gas exchange in compacted soils. The strongest effect was observed in the severely compacted wheel tracks where air and water conductivities were reduced permanently to 10% or even lower of the original conductivities of undisturbed soils. Very slow drainage in combination with a dramatically reduced gas permeability led to unfavorable soil conditions in severely disturbed traffic lanes reflecting the changes in the total bacterial community structures at both soil depths. Additionally, microbial biomass C tended to be lower in compacted soil. Our results indicate that the type of severe treatments imposed at these forest sites may have strong adverse effects on long-term soil sustainability.     

Radio detections during two state transitions of the intermediate-mass black hole HLX-1

Relativistic jets are streams of plasma moving at appreciable fractions of the speed of light. They have been observed from stellar-mass black holes (~3 to 20 solar masses, M(⊙)) as well as supermassive black holes (~10(6) to 10(9) M(⊙)) found in the centers of most galaxies. Jets should also be produced by intermediate-mass black holes (~10(2) to 10(5) M(⊙)), although evidence for this third class of black hole has, until recently, been weak. We report the detection of transient radio emission at the location of the intermediate-mass black hole candidate ESO 243-49 HLX-1, which is consistent with a discrete jet ejection event. These observations also allow us to refine the mass estimate of the black hole to be between ~9 × 10(3) M(⊙) and ~9 × 10(4) M(⊙).     

Do changes in carbon allocation account for the growth response to potassium and sodium applications in tropical Eucalyptus plantations?

Understanding the underlying mechanisms that account for the impact of potassium (K) fertilization and its replacement by sodium (Na) on tree growth is key to improving the management of forest plantations that are expanding over weathered tropical soils with low amounts of exchangeable bases. A complete randomized block design was planted with Eucalyptus grandis (W. Hill ex Maiden) to quantify growth, carbon uptake and carbon partitioning using a carbon budget approach. A combination of approaches including the establishment of allometric relationships over the whole rotation and measurements of soil CO(2) efflux and aboveground litterfall at the end of the rotation were used to estimate aboveground net production (ANPP), total belowground carbon flux and gross primary production (GPP). The stable carbon isotope (δ(13)C) of stem wood α-cellulose produced every year was used as a proxy for stomatal limitation of photosynthesis. Potassium fertilization increased GPP and decreased the fraction of carbon allocated belowground. Aboveground net production was strongly enhanced, and because leaf lifespan increased, leaf biomass was enhanced without any change in leaf production, and wood production (P(W)) was dramatically increased. Sodium application decreased the fraction of carbon allocated belowground in a similar way, and enhanced GPP, ANPP and P(W), but to a lesser extent compared with K fertilization. Neither K nor Na affected δ(13)C of stem wood α-cellulose, suggesting that water-use efficiency was the same among the treatments and that the inferred increase in leaf photosynthesis was not only related to a higher stomatal conductance. We concluded that the response to K fertilization and Na addition on P(W) resulted from drastic changes in carbon allocation.     

Babesia divergens: cloning of a Ran binding protein 1 homologue

Babesia divergens is an Apicomplexa transmitted to bovines by its acarian vector, the tick I. ricinus. Babesia divergens merozoites have an intraerythrocytic development in the blood of infected mammals. The nucleocytoplasmic transport system in this parasite is not yet characterized and no protein involvement in such transport has been described. In this report, we describe the cloning of a protein that shares important homologies with Ran binding protein 1. This protein in Eukaryote belongs to the nucleocytoplasmic transport system.     

家禽的免疫抑制及其解决方案

1免疫抑制的发生 　　疾病从病鸡传染给健康鸡可以通过多种途径进行,如:鼻、眼的分泌物,羽毛囊细胞,粪便,精液等.并且传染通常在病鸡出现症状之前就已经发生了.而通过疫苗的免疫接种,可以保护家禽的免疫系统、呼吸系统、消化系统和生殖系统免受感染,甚至可以通过蛋内接种或者1日龄免疫接种对强毒的早期感染进行及早预防.但是免疫抑制对疫苗的免疫接种效果有很大的破坏作用,使疫苗的保效果降低,甚至造成免疫失败.……     

Effects of suture tension during unilateral cricoarytenoid lateralization on canine laryngeal resistance in vitro

Objective— To evaluate the effect of abduction suture tension for unilateral arytenoid lateralization on laryngeal resistance. Study Design— Experimental study. Animals— Canine cadaver larynges (n=16). Methods— Laryngeal resistance was calculated in all specimens with the epiglottis in open and closed positions. Left cricoarytenoid lateralization was performed under low or high suture tension, and laryngeal resistance was re‐calculated. The effects of suture tension on laryngeal resistance were evaluated by repeated measures ANOVA. Results— Cricoarytenoid lateralization under low or high suture tension significantly reduced laryngeal resistance with the epiglottis in an open or closed position. There was no difference in laryngeal resistance with an open epiglottis between the low‐tension (1.00±0.0001 cm H₂O/L/s) and high‐tension (1.10±0.35 cm H₂O/L/s) groups (P=.33). The low‐tension group (22.80±14.20 cm H₂O/L/s) had significantly greater laryngeal resistance than the high‐tension group (8.45±4.00 cm H₂O/L/s) with a closed epiglottis (P=.016). There was no difference in laryngeal resistance with a closed epiglottis for the low‐tension group before (34.30±36.50 cm H₂O/L/s) and after (22.80±14.20 cm H₂O/L/s; P=.42) arytenoid lateralization. Conclusions— Cricoarytenoid lateralization under low suture tension significantly reduced laryngeal resistance with an open epiglottis, but resulted in a significantly greater resistance with a closed epiglottis than cricoarytenoid lateralization under high suture tension. Clinical Relevance— Clinically, use of a low‐tension suture for cricoarytenoid lateralization may provide an adequate decrease in open‐epiglottis laryngeal resistance to alleviate clinical signs, while maintaining enough closed‐epiglottis laryngeal resistance to reduce the risk of postoperative aspiration pneumonia.     

Inverse models to analyze the spatiotemporal variations of chemical weathering fluxes in a granito-gneissic watershed: Mule Hole, South India

Water-rock reactions are driven by the influx of water, which are out of equilibrium with the mineral assemblage in the rock. Here a mass balance approach is adopted to quantify these reactions. Based on field experiments carried out in a granito-gneissic small experimental watershed (SEW), Mule Hole SEW (~ 4.5 km²), quartz, oligoclase, sericite, epidote and chlorite are identified as the basic primary minerals while kaolinite, goethite and smectite are identified as the secondary minerals. Observed groundwater chemistry is used to determine the weathering rates, in terms of ‘Mass Transfer Coefficients’ (MTCs), of both primary and secondary minerals.Weathering rates for primary and secondary minerals are quantified in two steps. In the first step, top red soil is analyzed considering precipitation chemistry as initial phase and water chemistry of seepage flow as final phase. In the second step, minerals present in the saprolite layer are analyzed considering groundwater chemistry as the output phase. Weathering rates thus obtained are converted into weathering fluxes (Q_weathering) using the recharge quantity.Spatial variability in the mineralogy observed among the thirteen wells of Mule Hole SEW is observed to be reflected in the MTC results and thus in the weathering fluxes. Weathering rates of the minerals in this silicate system varied from few 10 μmol/L (in case of biotite) to 1000 s of micromoles per liter (calcite). Similarly, fluxes of biotite are observed to be least (7 ± 5 mol/ha/yr) while those of calcite are highest (1265 ± 791 mol/ha/yr). Further, the fluxes determined annually for all the minerals are observed to be within the bandwidth of the standard deviation of these fluxes. Variations in these annual fluxes are indicating the variations in the precipitation. Hence, the standard deviation indicated the temporal variations in the fluxes, which might be due to the variations in the annual rainfall. Thus, the methodology adopted defines an inverse way of determining weathering fluxes, which mainly contribute to the groundwater concentration.     

Radial growth and characterization of juvenile and adult wood in plantation grown okoumé (Aucoumea klaineana Pierre) from Gabon

The fast-growing hardwood, okoumé (Aucoumea klaineana Pierre), is a major forest species in Gabon and is used principally for making plywood, but research into the growth and quality of this wood is scanty. Trees from natural forests are favoured for production, yet little information exists on wood characteristics from plantation trees. Therefore, we carried out a dendrochronological study along with measurements of wood longitudinal modulus of elasticity (E _L ), density (D _w ), dimensional stability parameters (longitudinal, radial and tangential shrinkage and fibre saturation point) and fibre cell morphology to determine if these properties were related to age in trees from two plantations. We then used segmented regression analysis to define the limit (breakpoint) between juvenile (JW) and adult wood (AW). Using monthly precipitation data, we were able to determine that one growth ring is formed per year, composed of a large light coloured ring formed during the long rainy season and a thick, dark band formed during the major dry season. However, thinner bands, analogous to false rings, may also form during the short dry and short rainy seasons. Ring width decreases from the pith to the bark, and the breakpoint between JW and AW was at 19 years old when trees from both plantations were pooled together. No differences in D _w or radial and tangential shrinkage occurred with cambial age. E _L increased significantly up to the cambial age of 12–14 years, after which the increase with age was only slight and no breakpoint between JW and AW was found. With regard to mean longitudinal shrinkage, AW was found to form after the age of 13 years but fibre cell length was significantly longer after the age of 14.5 and 20 years, depending on the plantation of origin. Therefore, the boundary between JW and AW in plantation grown okoumé occurs between the ages of 13 and 20 years, depending on the characteristic examined.     

Consumption of a functional fermented milk containing collagen hydrolysate improves the concentration of collagen-specific amino acids in plasma

Clinical studies have shown that collagen hydrolysate (CH) may be able to protect joints from damage, strengthen joints, and reduce pain from conditions like osteoarthritis. CH is a collection of amino acids and bioactive peptides, which allows for easy absorption into the blood stream and distribution in tissues. However, although various matrices have been studied, the absorption of specific amino acids from CH added to a fresh fermented milk product (FMP) was not studied. The primary objective of the present study was to compare the plasma concentrations of four representative amino acids from the CH (glycine, proline, hydroxyproline, and hydroxylysine) contained in a single administration of a FMP with that of a single administration of an equal amount of neat hydrolyzed collagen. These four amino acids were chosen because they have already been used as markers of CH absorption rate and bioavailability. This was a single-center, randomized open, and crossover study with two periods, which was performed in 15 healthy male subjects. The subjects received randomly and in fasted state a single dose of product 1 (10 g of CH in 100 mL of FMP) and product 2 (10 g of CH dissolved in 100 mL of water) separated by at least 5 days. After administration, the subjects were assessed for plasma concentrations of amino acids and for urine concentrations of hydroxyproline. After FMP administration, mean values of the maximal concentration (Cmax) of the four amino acids were greater than after ingredient administration (p < 0.05). This effect was related to an increased Cmax of proline (p < 0.05). In conclusion, because of their physicochemical characteristics, the fermentation process, and the great homogeneity of the preparation, this milk product improves the plasma concentration of amino acids from CH, that is, proline. The present study suggests an interesting role for FMP containing CH to improve the plasmatic availability of collagen-specific amino acids. Hence, this FMP product could be of potential interest in the management of joint diseases.