全文获取类型
收费全文 | 193篇 |
免费 | 16篇 |
专业分类
林业 | 11篇 |
农学 | 2篇 |
50篇 | |
综合类 | 15篇 |
农作物 | 6篇 |
水产渔业 | 11篇 |
畜牧兽医 | 102篇 |
园艺 | 1篇 |
植物保护 | 11篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 15篇 |
2019年 | 13篇 |
2018年 | 6篇 |
2017年 | 10篇 |
2016年 | 8篇 |
2015年 | 6篇 |
2014年 | 11篇 |
2013年 | 18篇 |
2012年 | 22篇 |
2011年 | 20篇 |
2010年 | 12篇 |
2009年 | 6篇 |
2008年 | 10篇 |
2007年 | 14篇 |
2006年 | 4篇 |
2005年 | 4篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 8篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1995年 | 1篇 |
排序方式: 共有209条查询结果,搜索用时 15 毫秒
61.
Narita M Young AR Arakawa S Samarajiwa SA Nakashima T Yoshida S Hong S Berry LS Reichelt S Ferreira M Tavaré S Inoki K Shimizu S Narita M 《Science (New York, N.Y.)》2011,332(6032):966-970
Protein synthesis and autophagic degradation are regulated in an opposite manner by mammalian target of rapamycin (mTOR), whereas under certain conditions it would be beneficial if they occurred in unison to handle rapid protein turnover. We observed a distinct cellular compartment at the trans side of the Golgi apparatus, the TOR-autophagy spatial coupling compartment (TASCC), where (auto)lysosomes and mTOR accumulated during Ras-induced senescence. mTOR recruitment to the TASCC was amino acid- and Rag guanosine triphosphatase-dependent, and disruption of mTOR localization to the TASCC suppressed interleukin-6/8 synthesis. TASCC formation was observed during macrophage differentiation and in glomerular podocytes; both displayed increased protein secretion. The spatial coupling of cells' catabolic and anabolic machinery could augment their respective functions and facilitate the mass synthesis of secretory proteins. 相似文献
62.
Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
63.
Heiko Nathues Henrike Woeste Stefanie Doehring Anna S Fahrion Marcus G Doherr Elisabeth grosse Beilage 《Acta veterinaria Scandinavica》2013,55(1):30
Background
Mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia mainly occurring in fattening pigs. It is assumed that horizontal transmission of the pathogen during nursery and growing phase starts with few suckling pigs vertically infected by the sow. The aim of the present study was the exploration of the herd prevalence of M. hyopneumoniae infections in suckling pigs followed by an investigation of various herd specific factors for their potential of influencing the occurrence of this pathogen at the age of weaning.Results
In this cross-sectional study, 125 breeding herds were examined by taking nasal swabs from 20 suckling pigs in each herd. In total, 3.9% (98/2500) of all nasal swabs were tested positive for M. hyopneumoniae by real-time PCR. Piglets tested positive originated from 46 different herds resulting in an overall herd prevalence of 36.8% (46/125) for M. hyopneumoniae infection in pigs at the age of weaning. While the herds were epidemiologically characterized, the risk for demonstration of M. hyopneumoniae was significantly increased, when the number of purchased gilts per year was more than 120 (OR: 5.8), and when the number of farrowing pens per compartment was higher than 16 (OR: 3.3). In herds with a planned and segregated production, where groups of sows entered previously emptied farrowing units, the risk for demonstration of M. hyopneumoniae in piglets was higher in herds with two or four weeks between batches than in herds with one or three weeks between batches (OR: 2.7).Conclusions
In this cross-sectional study, several risk factors could be identified enhancing the probability of breeding herds to raise suckling pigs already infected with M. hyopneumoniae at the time of weaning. Interestingly, some factors (farrowing rhythm, gilt acclimatisation issues) were overlapping with those also influencing the seroprevalences among sows or the transmission of the pathogen between older age groups. Taking the multifactorial character of enzootic pneumonia into account, the results of this study substantiate that a comprehensive herd specific prevention programme is a prerequisite to reduce transmission of and disease caused by M. hyopneumoniae. 相似文献64.
Stefanie Anne van Bergeijk José Antonio Paullada Salmerón Ana Isabel López Pérez José Moreno José Pedro Cañavate Mercedes García‐González 《Aquaculture Research》2013,44(11):1724-1730
Rotifers, used in aquaculture as feed for early larval stages, are poor in carotenoids compared with copepods, the natural most common prey item of marine fish larvae. A minimal sufficient level of carotenoids in fish larvae may be essential for a correct development. The freshwater microalga Muriellopsis sp. has been identified as a potential source of the carotenoid lutein with possible application in aquaculture. Lutein is a potent antioxidant, with potentially beneficial effects as a carotenoid for fish larvae. This study describes the lutein enrichment of the rotifer Brachionus sp. when incubated with homogeneously dispersed suspensions of freeze‐dried, lutein‐rich Muriellopsis sp. in seawater. A short enrichment time (2 or 2.5 h) resulted in a higher lutein content of the rotifers than a long enrichment time (13.5 or 24 h). Although the concentration of lutein in enriched rotifers, 0.06–0.16 mg (g dry weight)?1 was low compared with its content in Muriellopsis sp. [about 3 mg (g dry weight)?1], it was in the range reported for natural copepod populations. Therefore, we conclude that freeze‐dried biomass of Muriellopsis sp. can be used to enrich rotifers with lutein, providing an adequate level of this carotenoid for marine fish larvae. 相似文献
65.
66.
Ohlerth S Becker-Birck M Augsburger H Jud R Makara M Braun U 《Research in veterinary science》2012,93(1):7-12
There is increasing evidence that activation of innate immunity, in animals and man, by live vaccines, sub-unit vaccines or synthetic or non-synthetic stimulants can induce a profound and rapidly induced resistance to pathogens, including infectious agents that are unrelated to the stimulating antigen or agent. We review the evidence for this phenomenon and present the proposition that this approach might be used to stimulate immunity during the life of the animal when susceptibility to infection is high and when normal vaccination procedures may be inappropriate. 相似文献
67.
Journal of Plant Diseases and Protection - In the Lower Elbe region of Northern Germany, low levels of fungicide resistance were recorded among Botrytis isolates obtained from blossom-end rot... 相似文献
68.
69.
Christina-Luise Ross Verena Wilken Stefanie Krück Kerstin Nielsen Karen Sensel-Gunke Frank Ellmer 《Archives of Agronomy and Soil Science》2017,63(14):1939-1950
Fermentation in combination with subsequent composting of biowaste is a preferred method for municipalities to recycle organic byproducts and transform them into useful end products for soil amelioration or plant fertilization. These compost products, especially if obtained from household wastes, can be a source of hazardous components, e.g. heavy metals, pathogens, synthetic chemicals or toxic organic compounds. An avoidance test with two earthworm species (Eisenia fetida and Aporrectodea caliginosa) was conducted to assess the impact of differently processed biowaste based digestate products on soil invertebrates. Body weight changes were recorded, as well as differences in effects on adult versus juvenile earthworms.
While E. fetida showed no avoidance towards the digestate products (negative avoidance, meaning that the tested products were preferred, of 80 to 100 %), A. caliginosa rejected the crude biowaste digestate compost in higher concentrations (avoidance of 45 %), but not the agglomerated or pelletized variants. A clear weight gain of up to 25 % was observed only for individuals of E. fetida. The developmental stages of the worms were not crucial for the outcome of the avoidance test.
Based on the results of this study the application of biowaste products on arable land cannot be recommended without constraints. 相似文献
70.
Stefanie Reim Frank Lochschmidt Anke Proft Heike Wolf Heino Wolf 《Genetic Resources and Crop Evolution》2017,64(5):1075-1085
The present study investigated genetic diversity, structure and hybridization in a collection of the endangered wild pear species Pyrus pyraster (L.) Burgd. A total of 278 putative ‘true type’ P. pyraster trees originating from seven populations in the federal state of Saxony in Germany were analyzed along with 35 pear cultivars commonly cultivated in Saxony. The genetic analysis was performed using nine nuclear microsatellite markers (ncSSR) and two paternally inherited chloroplast marker (cpDNA) amplifying in the intergenic spacer region trnQ–rps16 and the intron region rps16. On basis of the ncSSR dataset after STRUCTURE analysis 80 % of the wild pear individuals were assigned as ‘true type’ P. pyraster genotypes. The cpDNA analysis showed shared haplotypes in P. pyraster and P. communis but with an unequal frequency in both species. The analysis of molecular variance resulted in a moderate (ncSSR) and great (cpDNA) variation among ‘true type’ P. pyraster and the pear cultivars. The genetic diversity in the ‘true type’ P. pyraster populations was still high and the genetic structure between the populations low (ncSSR and cpDNA) indicating a genetic exchange between the populations by pollen and seeds. The clear discrimination between the P. pyraster and P. communis confirms our expectation of the existence of ‘true type’ P. pyraster individuals in the study area. The existing genetic integrity and the high genetic diversity argue for the implementation of preservation measures in P. pyraster. 相似文献