Cover Image,Volume 17, Issue 4

The cover image is based on the SHORT COMMUNICATION Clinical outcomes, ultrastructure and immunohistochemical features of canine high‐grade olfactory neuroblastoma by Molly E. Church et al., https://doi.org/10.1111/vco.12512 .

     

Effect of conservation agriculture on stratification of soil organic matter under cereal-based cropping systems

ABSTRACT

Degradation of soil quality caused by conventional tillage practices is a major concern for the sustainability of rice-wheat cropping systems in South Asian region. Therefore, suitable conservation agriculture (CA) practices are required. This study investigates the stratification and storage of soil organic carbon (SOC) and total nitrogen (TN) as affected by eight years of different CA practices in the North-West Indo-Gangetic Plains of India. There were four treatments: (1) conventionally tilled rice-wheat cropping system, (2) reduced-till CA-based rice-wheat-mungbean system, (3) no-till CA-based rice-wheat-mungbean system, and (4) no-till CA-based maize-wheat-mungbean system. The mean stratification ratio (SR) (i.e. a ratio of the concentrations of SOC and TN in the soil surface to those in a deeper layer) of SOC and TN for 0–5:5–10, 10–15, 15–20, 20–25 and 25–30 cm were found higher (> 2) under CA practices compared to intensive tillage-based conventional agricultural practice (< 2). No-till CA-based rice-wheat-mungbean system stored the highest amount of SOC (25.32 Mg ha^?1) whereas reduced till CA-based rice-wheat-mungbean system stored highest amount of TN (3.21 Mg ha^?1) at 0–30 cm soil depth. This study shows that CA stratifies SOC and TN and helps to enhance SOC sequestration and soil quality.     

Effect of dietary resveratrol in ameliorating aflatoxin B1‐induced changes in broiler birds

Consumption of aflatoxin B1 (AFB1) contaminated feed by poultry affects the health of broiler birds causing severe economic losses. The use of phytochemicals is a safe, effective, alternative and practical approach to combat the toxic effect of AF in broilers. Resveratrol, a polyphenol derived from red grapes, berries and peanuts, exerts anti‐inflammatory, antioxidant and immunomodulatory effects. Our study was aimed at evaluating the possible protective effects of resveratrol against the adverse effects of AFB1 in broiler birds. A feeding trial of 42 days of duration was undertaken in a completely randomized design with five dietary treatments: G1‐AFB1(1.0 ppm); G2‐CTR (basal diet alone); G3‐AFB1(1.0 ppm)+Resv 0.5%; G4‐AFB1(1.0 ppm)+Resv 1%; and G5‐Resv 1%. Gain in body weight (BWG) and feed intake (FI) was observed to be highest (p < 0.05) in the AFB1 birds followed by the control group. Feed conversion ratio was lowest in G2‐CTR birds and failed to record any significant variation (p > 0.05) between groups as well as within groups. Birds fed resveratrol at both 0.5% and 1.0% levels in combination with AFB1 as well as alone along with basal diet had lower BWG and FI between the fourth and fifth week and also at the fifth week (p < 0.05). No variation (p > 0.05) was obtained in the FCR of AFB1 and resveratrol group of broiler birds. AFB1 feeding significantly increased the activities of aspartate‐(AST) and alanine‐(ALT) amino transferase, superoxide dismutase (SOD) and catalase (CAT) activities (p < 0.05) but lowered glucose, cholesterol and triglyceride levels in serum. Supplementation of resveratrol helped in increasing the activities of the oxidative enzymes and in improving the plasma total antioxidant capacity (TAOC) and total protein (TP) significantly (p < 0.05) and protein values. The livers of AFB1 group showed degeneration of hepatocytes, bile duct hyperplasia and microgranuloma formation. In resveratrol supplemented birds, the severity and degree of the liver lesions was far less. Apoptotic proteins failed to show any variation in expression between AFB1, control and resveratrol group of birds. The inclusion of resveratrol in broiler diets enhanced antioxidant status of birds indicating the protective effect of resveratrol against AFB1‐induced toxicity. So, we advice use of resveratrol as a feed additive to control aflatoxicosis in poultry farms.     

Alleviating cancer drug toxicity by inhibiting a bacterial enzyme

The dose-limiting side effect of the common colon cancer chemotherapeutic CPT-11 is severe diarrhea caused by symbiotic bacterial β-glucuronidases that reactivate the drug in the gut. We sought to target these enzymes without killing the commensal bacteria essential for human health. Potent bacterial β-glucuronidase inhibitors were identified by high-throughput screening and shown to have no effect on the orthologous mammalian enzyme. Crystal structures established that selectivity was based on a loop unique to bacterial β-glucuronidases. Inhibitors were highly effective against the enzyme target in living aerobic and anaerobic bacteria, but did not kill the bacteria or harm mammalian cells. Finally, oral administration of an inhibitor protected mice from CPT-11-induced toxicity. Thus, drugs may be designed to inhibit undesirable enzyme activities in essential microbial symbiotes to enhance chemotherapeutic efficacy.     

Three co-located resistance genes confer resistance to leaf rust and stripe rust in wheat variety Borlaug 100

Leaf rust(LR) and stripe rust(YR) are important diseases in wheat producing areas worldwide and cause severe yield losses under favorable environmental conditions when susceptible varieties are grown. We determined the genetic basis of resistance to LR and YR in variety Borlaug 100 by developing and phenotyping a population of 198 F6 recombinant inbred lines derived from a cross with the susceptible parent Apav#1. LR and YR phenotyping were conducted for 4 and 3 seasons, respectively, at CIMMYT research stations in Mexico under artificial epidemics. Mendelian segregation analyses indicated that 3–5 LR and 2 YR genes conferred resistance in Borlaug 100. Lr46/Yr29(1 BL), Yr17(2 AS) and Yr30(3 BS) were present in the resistant parent and segregated in the RIL population based on characterization by molecular markers linked to these genes. When present alone, Lr46/Yr29 caused average 13% and 16% reductions in LR and YR severities, respectively, in RILs. Similarly, Yr17 and Yr30 reduced YR severities by 57% and 11%, respectively. The Yr30 and the Yr17 translocation were also associated with 27% and 14% reductions, respectively, in LR severity, indicating that the 3 BS and 2 AS chromosomal regions likely carry new slow rusting LR resistance genes, temporarily designated as Lr B1 and Lr B2, respectively. Additive effects of Yr30*Yr17, Yr29*Yr17 and Yr29*Yr30 on YR and LR were significant and reduced YR severities by 56%,55%, and 45%, respectively, and LR severities by 34%, 40%, and 45%, respectively. Furthermore, interaction between the three genes was also significant, with mean reductions of 70% for YR and 54% for LR severities. Borlaug 100, or any one of the 21 lines with variable agronomic traits but carrying all three colocated resistance loci, can be used as resistance sources in wheat breeding programs.     

The management of bovine fractures: a review of 59 cases

Current trends in bovine fracture repair are reviewed with reference to 59 cases. Thirty-one cases were managed surgically, 14 by external fixation, and six by stall confinement. Eight animals were slaughtered without treatment being attempted. A successful outcome was achieved in 90 per cent of the surgically treated cases, compared with 57 per cent for those treated by external fixation methods.     

Induction of triploidy and tetraploidy in stinging catfish, Heteropneustes fossilis (Bloch), using heat shock

Induction of triploidy and tetraploidy was attempted in Heteropneustes fossilis using heat shock. The optimal age of zygote, temperature level and duration of thermal shock required for effective induction of triploidy and tetraploidy was investigated in a series of experiments. A maximum of 82±7% triploids (3n=87) were obtained when fertilized eggs (2.5‐min old) were heat shocked at 40°C for 4‐min duration. A maximum of 40±8% tetraploids (4n=116) was obtained when the fertilized eggs (30‐min old) were heat shocked at 40°C for 4‐min duration. The triploid and tetraploid red blood cells (RBCs) nucleus volumes were 1.4 and 2.1 times greater, respectively, than that of the diploid RBC nucleus.