Identification of Chlamydia gallinacea in a parrot and in free‐range chickens in Australia

Chlamydia gallinacea is a recently described bacterial species in a genus known to infect and cause disease in animals and humans. Our report describes the identification of C. gallinacea infection in free‐range laying chickens (Gallus gallus) in Australia, and the identification of C. gallinacea infection in a parrot, a wild Australian galah (Eolophus roseicapillus). There is currently little knowledge of the effects of C. gallinacea infection on avian hosts, but it has been linked to respiratory disease in humans and could potentially cause similar disease in other species. Our report highlights the need for further study and surveillance of Chlamydia species in both wild and domestic hosts in Australia.     

Qualitative and quantitative ultrasound attributes of maternal‐foetal structures in pregnant ewes

The aim of this study was to examine foetal organs and placental tissue to establish a correlation between the changes in the composition of these structures associated with their maturation and the ultrasonographic characteristics of the images. Twenty‐four pregnant ewes were included in the study. Ultrasonography assessments were performed in B‐mode, from the ninth gestational week until parturition. The lungs, liver and kidneys of foetuses and placentomes were located in transverse and longitudinal sections to evaluate the echogenicity (hypoechoic, isoechoic, hyperechoic or mixed) and echotexture (homogeneous and heterogeneous) of the tissues of interest. For quantitative evaluation of the ultrasonographic characteristics, it was performed a computerized image analysis using a commercial software (Image ProPlus^®). Mean numerical pixel values (NPVs), pixel heterogeneity (standard deviation of NPVs) and minimum and maximum pixel values were measured by selecting five circular regions of interest in each assessed tissue. All evaluated tissues presented significant variations in the NPVs, except for the liver. Pulmonary NPVmean, NPVmin and NPVmax decreased gradually through gestational weeks. The renal parameters gradually decreased with the advancement of the gestational weeks until the 17th week and later stabilized. The placentome NPVmean, NPVmin and NPVmax decreased gradually over the course of weeks. The hepatic tissue did not show echogenicity and echotexture variations and presented medium echogenicity and homogeneous echotexture throughout the experimental period. It was concluded that pixels numerical evaluation of maternal‐foetal tissues was applicable and allowed the identification of quantitative ultrasonographic characteristics showing changes in echogenicity related to gestational age.     

Differentiation between mated and virgin males inSpodoptera Litura

During mating, the male ofSpodoptera litura F. (Noctuidae: Lepidoptera) deposits a portion of the secretions from the primary simplex into the bursa of the female, after which the simplex becomes flaccid. Subsequently, the secretions in the primary simplex remain either colorless or light yellow. The newly mated males can not be differentiated from 0- or 1-day-old virgins, as the color of the simplex is quite similar in both cases. In such situations the width of the simplex can be used as a quantitative character to differentiate mated males from virgins. In laboratory studies, the width of the primary simplex was found to be less than 435 μm in 96.7% of mated males, whereas it was more than 451 μm in 94.65% of virgin males. The percentage falling in the range of 435-451 μm is only 3.31 and 5.35 in mated and virgin males, respectively. In the trapped males also, the percentage of males with this range is only 8.63.     

Toxoplasma gondii antibody in domestic cats in Melbourne

OBJECTIVES: To determine the prevalence of Toxoplasma gondii in a population of domestic cats in Melbourne. DESIGN: An ELISA assay was used to measure T gondii antibody titres in 103 cats from north-eastern Melbourne. Cats were obtained from outer suburban areas (less than 30 km from the Melbourne GPO) and from rural areas (more than 30 km from the Melbourne GPO). RESULTS: Thirty-nine percent of cats were positive for T gondii IgG. Older cats tended to have higher antibody titres. There was no significant difference in the T gondii antibody titres between males and females, or between cats living in urban areas and cats from rural areas. CONCLUSIONS: A significant proportion of cats from Melbourne have been exposed to Toxoplasma. This may have implications for the health of wildlife and humans.     

The relationship of training milestones with racing success in a population of Standardbred horses in New Zealand

AIM: To gather information on the repeatability of a faecal nematode egg count (FEC) reduction (FECR) test (FECRT), evaluating both different methods of calculating efficacy and variations within a method, in order to supply veterinarians and other advisors with sufficient information to apply some level of confidence around a diagnosis of anthelmintic resistance based on FECRT results.

METHODS: Two commercial sheep farms were selected on the basis of having previously recorded FECR <95% after treatment with ivermectin (Farm 1) or albendazole (Farm 2). On each farm at least 250 lambs, managed as a single mob, were individually ear-tagged and sampled for FEC. The resulting counts were used, 3—4 days later, to sort the lambs into 24 groups of 10. First, the animals were split into three groups of 80, having high, medium or low FEC. Second, within each of these groups the 80 animals were further divided into four replicate mobs of 20 (each with the same mean count). Third, each of these replicates was further split into two groups of 10: those that would be drenched and those that would remain as untreated controls. All animals were again faecal-sampled and those in the drenched groups were dosed, using a syringe, to their individual liveweight, with ivermectin (Farm 1) or albendazole (Farm 2). Ten days after treatment all animals were individually faecal- sampled again. FEC and larval cultures were undertaken for all 24 groups from both pre- and post-treatment samples. Ef- ficacy (FECR) of the undifferentiated FECRT was calculated using three different equations, and efficacy by genus was also calculated.

RESULTS: Calculated efficacies differed between equations, and the equation which did not utilise an untreated control yielded significantly lower efficacy estimates on both farms. Faecal cultures varied considerably in the proportions of parasite genera recovered. In general, this did not differ between FEC groups, except on Farm 1 where Haemonchus spp were more common and Cooperia spp less common in high-FEC samples. Estimated efficacies against individual genera varied considerably or very little, depending on the level of resistance. On both farms, differing proportions of tests against some genera passed or failed FECRTs based on a threshold pass mark of ≥95% FECR.

CONCLUSION: There was considerable variability in the outcomes of FECRTs and in larval culture results. Caution is warranted in interpreting the results of FECRTs when efficacy values fall into the 90—95% range. Further, the possibility of a test returning a false-negative result is raised, indicating that even an efficacy estimated ≥95% may not guarantee the absence of resistant parasites.     

Nutritional evaluation of wheat straw treated with Crinipellis sp. in Sahiwal calves

Wheat straw was subjected to solid-state fermentation (SSF) with lignolytic white-rot fungus (WRF) Crinipellis sp. for 5 days to improve the nutritive value and digestibility. The fungal treatment caused a significant (P?<?0.05) decrease in cell wall constituents viz., neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose, lignin, and cellulose to the extent of 10.4, 11.2, 8.7, 8.7, and 12.1 %, respectively, with increase (P?<?0.05) in crude protein (CP) (51.6%) and ash (25.8%) contents in fungal treated wheat straw (FT-WS) than untreated wheat straw (UT-WS). Further, in vitro gas production, in vitro true dry matter digestibility and in vitro true organic matter digestibility at 48 h, metabolizable energy (ME) content, microbial biomass production, and short-chain fatty acids synthesis were significantly (P?<?0.05) higher in FT-WS. In vivo feeding trial in 10 Sahiwal calves (8–12 months) comprised of (1) control group (T1) fed with ad libitum chopped UT-WS and (2) treatment group (T2) offered with ad libitum chopped FT-WS, in addition to supplementation of groundnut cake and green berseem (Trifolium alexandrium) forage to both groups. Digestibility of nutrients for dry matter (DM), organic matter, CP, NDF, ADF, hemicellulose, cellulose, and total carbohydrates were significantly (P?<?0.05) higher in T2 compared to T1. Moreover, daily DM (P?<?0.05), digestible crude protein (P?<?0.01), and ME intakes were also higher (P?<?0.05) in group T2 with higher (P?<?0.05) nitrogen (N) retention, which resulted in significantly (P?<?0.05) higher average daily gain in body weight (135 vs. 102 g/day). It was concluded that SSF with WRF Crinipellis sp. holds potential in upgrading the nutritional worth of wheat straw for feeding growing calves.     

Subsurface drainage to combat waterlogging and salinity in irrigated lands in India: Lessons learned in farmers’ fields

The introduction of irrigated agriculture in the arid and semi-arid regions of India has resulted in the development of the twin problem of waterlogging and soil salinization. It is estimated that nearly 8.4 million ha is affected by soil salinity and alkalinity, of which about 5.5 million ha is also waterlogged. Subsurface drainage is an effective tool to combat this twin problem of waterlogging and salinity and thus to protect capital investment in irrigated agriculture and increase its sustainability. In India, however, subsurface drainage has not been implemented on a large scale, in spite of numerous research activities that proved its potential. To develop strategies to implement subsurface drainage, applied research studies were set-up in five different agro-climatic sub-regions of India. Subsurface drainage systems, consisting of open and pipe drains with drain spacing varying between 45 and 150 m and drain depth between 0.90 and 1.20 m, were installed in farmers’ fields. The agro-climatic and soil conditions determine the most appropriate combination of drain depth and spacing, but the drain depths are considerably shallower than the 1.75 m traditionally recommended for the prevailing conditions in India. Crop yields in the drained fields increased significantly, e.g. rice with 69%, cotton with 64%, sugarcane with 54% and wheat with 136%. These increases were obtained because water table and soil salinity levels were, respectively, 25% and 50% lower than in the non-drained fields. An economic analysis shows that the subsurface drainage systems are highly cost-effective: cost-benefit ratios range from 1.2 to 3.2, internal rates of return from 20 to 58%, and the pay-back periods from 3 to 9 years. Despite these positive results, major challenges remain to introduce subsurface drainage at a larger scale. First of all, farmers, although they clearly see the benefits of drainage, are too poor to pay the full cost of drainage. Next, water users’ organisations, not only for drainage but also for irrigation, are not well established. Subsurface drainage in irrigated areas is a collective activity, thus appropriate institutional arrangements for farmers’ participation and organisation are needed. Thus, to assure that drainage gets the attention it deserves, policies have to be reformulated.