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881.
Genomic evaluation using SNP‐ and haplotype‐based genomic relationship matrices in a closed line of Duroc pigs 下载免费PDF全文
Yoshinobu Uemoto Shuji Sato Takashi Kikuchi Sachiko Egawa Kimiko Kohira Hironori Sakuma Satoshi Miyashita Shinji Arata Takatoshi Kojima Keiichi Suzuki 《Animal Science Journal》2017,88(10):1465-1474
A simulation analysis and real phenotype analysis were performed to evaluate the impact of three different relationship matrices on heritability estimation and prediction accuracy in a closed‐line breeding of Duroc pigs. The numerator relationship matrix (NRM), single nucleotide polymorphism (SNP)‐based genomic relationship matrix (GRM) (GS), and haplotype‐based GRM (GH) were applied in this study. We used PorcineSNP60 genotype array data (38 114 SNPs) of 831 Duroc pigs with four selection traits. In both heritability estimation and prediction accuracy, the accuracy depended on the number of animals with records. For heritability estimation, a large difference in the results among three relationship matrices was not shown, but the trend of the estimated heritabilities between GRMs, that is GS < GH, was shown in this population. For the accuracy of prediction values in test animals, the accuracies of prediction values obtained by two GRMs were higher than that by the NRM in this population. The accuracies obtained by GRMs using animals with no records were lower than that by the NRM using animals with their performance records, but were close to that by the NRM using animals with full‐sib testing records. 相似文献
882.
Changes in the morphology and functions of vacuoles provide useful information about the mechanism of cell death. In the present study, we monitored the morphology and contents of vacuoles during the death of ray parenchyma cells in the conifer Cryptomeria japonica. In differentiating xylem, ray parenchyma cells had large central vacuoles. In sapwood, vacuoles in ray parenchyma cells contained proteins, an indication that one of the main functions of these vacuoles might be protein storage. A dramatic decrease in the protein content of some vacuoles was detected in the intermediate wood before the initiation of vacuole rupture. Although vacuole rupture was detected from the intermediate wood to the outermost heartwood, some vacuoles were obviously enlarged in the inner intermediate wood. Condensed nuclei were first observed after the rupture of these large vacuoles in ray parenchyma cells. It seems plausible that the autolysis of the contents of ray parenchyma cells might be caused by the rupture of the enlarged vacuoles in the inner intermediate wood. 相似文献
883.
Yoshihiko Hirayama Shunsuke Asano Ken’o Okayama Satoshi T. Ohki Motoaki Tojo 《Journal of General Plant Pathology》2018,84(1):12-19
Colletotrichum fructicola is a major causal agent among anthracnose pathogens of strawberry in Nara, Japan. We hypothesized that a wide range of weeds growing in and around strawberry fields are inoculum sources of the disease and investigated their potential as hosts of C. fructicola. We also examined the influence of herbicide treatment on C. fructicola sporulation on weeds. The fungus was detected on 31 of 541 (5.7%) leaves sampled from 13 weed species from 2005 to 2008. The fungus was most frequently isolated from leaves of Amaranthus blitum with an isolation frequency of 17.9%; inoculation of A. blitum with the pathogen caused brown leaf spots. Other weeds such as Digitaria ciliaris, Galinsoga ciliata, Solidago altissima, Erigeron annuus, and Sonchus oleraceus were found to harbor the fungus at lower rates (4.3–8.1%) without symptoms. C. fructicola formed acervuli on leaves of A. blitum, D. ciliaris, and S. oleraceus after plants were killed by a herbicide (glyphosate). These results demonstrated that infected weeds associated with strawberry cultivation are potential inoculum sources of C. fructicola, especially after herbicide treatment. 相似文献
884.
Satoshi Suyama Kazuhiro Oshima Masayasu Nakagami Atsushi Kawabata 《Fisheries Science》2011,77(2):223-233
We evaluated the seasonal changes in otolith and somatic growth of age-0 Pacific saury Cololabis saira in 223 fish collected between June and November 2002. We calculated the age in days of each individual by measuring otolith
growth increments under a scanning electron microscope. The age was correlated with body length and otolith radius. We also
observed seasonal changes in the rate of increase in body length and otolith radius and in the pattern of otolith growth.
Until August, both body length and otolith radius increased with age. Thereafter, the otolith radius continued to increase,
whereas the rate of somatic growth decreased. As a result, the ratio of otolith radius to body length increased. After August,
the percentage of otoliths with unreadable increments on their edge increased due to the formation of hyaline zones. Otoliths
grew both radially and in thickness until July, but gradually stopped growing in thickness after August. Beginning in October,
more than 80% of otoliths only grew radially. After August, the otolith not only continued growing but the morphological growth
pattern also changed. 相似文献
885.
Satoshi KAMOTO Masahiro SHINADA Daiki KATO Masaya TSUBOI Sho YOSHIMOTO Ryohei YOSHITAKE Shotaro ETO Namiko IKEDA Yosuke TAKAHASHI Yuko HASHIMOTO James CHAMBERS Kazuyuki UCHIDA Shinji YAMADA Mika K. KANEKO Ryohei NISHIMURA Yukinari KATO Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1795
Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology. 相似文献
886.
Murata K Nii R Sasaki E Ishikawa S Sato Y Sawabe K Tsuda Y Matsumoto R Suda A Ueda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(2):203-205
An adult male white eared-pheasant (Crossoptilon crossoptilon) at a Japanese zoo exhibited lethargy and emaciation. Microscopic examination of a blood smear revealed a haemosporidian parasitic infection. Based on the morphological characteristics and molecular analysis of the parasite, it was identified as Plasmodium (Bennettinia) juxtanucleare. This is the first report of P. juxtanucleare infection in bird species belonging to the genus Crossoptilon. Caution against avian malaria infection is required for the conservation of endangered bird species in zoos. 相似文献
887.
Fisheries Science - Identification of the population structure of harvested species is the first step in the management of wild fisheries. Although the marbled rockfish Sebastiscus marmoratus is a... 相似文献
888.
Shigenobu Namiki Hiroshige Tanaka Satoshi Katayama Osamu Funaki Ichiro Aoki Yoshioki Oozeki 《Fisheries Science》2010,76(6):951-959
In order to validate daily increment formation in otoliths of immature and adult Japanese anchovy Engraulis japonicus, three rearing experiments using chemical marking of otoliths were conducted on adult anchovy in summer 2004 and immature
anchovy in summer 2005 and in winter 2006. In the two experiments conducted in summer, the number of otolith microincrements
between alizarin complexone (ALC) marks showed that microincrements were formed daily. In the summer 2005 experiment, immature
anchovy under conditions of reduced daily food rations also showed daily microincrement formation. Average increment width
was 0.9 μm in adults and 1.8–3.1 μm in immature anchovy. In contrast, no clear increments were observed between ALC marks
on the otoliths from the experiment in winter 2006, and scanning electron microscope (SEM) observations failed to confirm
clear increment formation. We consider that low water temperatures (<13–14°C) restricted otolith growth and lowered the contrast
between the discontinuous and the incremental zones of the otolith increments. For age estimation of Japanese anchovy, clear
increments wider than about 1 μm in the otolith can be regarded as daily increments. However, daily age estimation of immature
and adult anchovy that experience low water temperatures in winter may be difficult due to the obscurity of the increments. 相似文献
889.
Okamoto Mineo Yap Minlee Roeroe Andreus Kakaskasen Nojima Satoshi Oyamada Kumi Fujiwara Shuichi Iwata Itaru 《Fisheries Science》2010,76(2):343-353
The growth of Acropora in Sekisei Lagoon was investigated in situ using ceramic coral settlement devices (CSDs) and marine blocks (MBs) with small
holes on their surfaces that were deployed prior to a mass spawning event. Acropora that had settled in 10-mm holes on the upper surface of the MBs were found to have grown inwards after 348 days post-mass
spawning. After 733 days, the colonies had grown outwards, encrusting the blocks and attaining a maximum diameter of 21.5 mm
(3.7 mm). CSDs that had been stacked above each other on frames were separated and observed in situ on 405 and 764 days after
mass spawning. After 405 days, the maximum diameter of the encrusting Acropora was 7.6 mm (±2.4 mm), which increased to 19.4 mm (±5.5 mm) after 764 days, with 30% of colonies extending short branches.
The length of the branches of Acropora grown on the CSDs fixed to the MBs 3 months after the mass spawning event exceeded the size of the encrusting portion of
the colony 629 days after spawning. The mortality of colonies between 1 and 2 years was slight, with the temperatures conducive
to coral bleaching that occurred during the study also having only a slight effect. 相似文献
890.
Shiro JIMI Arman SAPAROV Seiko KOIZUMI Motoyasu MIYAZAKI Satoshi TAKAGI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1933
Hypertrophic scars found on the human body rarely develop in experimental animals, possibly due to their looser skin structure. This makes it difficult to understand the genesis of scar lesions. Therefore, appropriate animal models are urgently needed. In this study, we established a novel experimental model of a scar-forming wound by resecting a small portion of the abdominal muscle wall on the lower center of the abdomen in C57BL/6N mice, which are exposed to contractive forces by the surrounding muscle tissue. As a low-tension control, a back skin excision model was used with a splint fixed onto the excised skin edge, and granulation tissue formed on the muscle fascia supported by the back skeleton. One week after the resection, initial healing reactions, such as fibroblast proliferation, occurred in both models. However, after 21 days, lesions with collagen-rich granulation tissues, which were also accompanied by multiple nodular/spherical-like structures, developed only in the abdominal wall model. These lesions were analogous to scar lesions in humans. Therefore, the animal model developed in this study is unique in that fibrous scar tissues form under physiological conditions without using any artificial factors and is valuable for studying the pathogenesis and preclinical treatment of scar lesions. 相似文献