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51.
Shiro Itoi Koki Ikeguchi Masaki Kaneniwa Ryuji Kuwahara Ichiro Ohara Noriko Ishida Michiaki Yamashita Shugo Watabe 《Fisheries Science》2007,73(2):429-439
ABSTRACT: Fast skeletal muscles of Japanese flounder Paralichthys olivaceus and red sea bream Pagrus major were examined for quantitative and qualitative changes of mitochondrial ATP synthase (Fo F1 -ATPase) in association with rearing temperatures. The specific activities of Fo F1 -ATPase from Japanese flounder reared at 10°C, 15°C and 25°C for 4 weeks were determined to be 81 ± 11, 74 ± 13 and 83 ± 11 nmol/min·mg mitochondrial protein, respectively. The corresponding activity from red sea bream reared at 8°C for 5 weeks was determined to be 65 ± 9 nmol/min·mg mitochondrial protein, which was higher than 33 ± 9 nmol/min·mg mitochondrial protein in fish reared at 23°C. The contents of α- and β-F1 -ATPase in total mitochondrial proteins were not significantly different between fish reared at different temperatures for the two fish species. However, the contents of β-F1 -ATPase in the total fast skeletal muscle extracts, prepared from Japanese flounder reared at 10°C, were 2.1- and 2.9-fold higher than those for fish reared at 15°C and 25°C, respectively. The corresponding content from red seabream reared at 8°C was 2.2-fold higher than that for fish reared at 23°C. Therefore, the changes in Fo F1 -ATPase depending on rearing temperatures were species-specific. 相似文献
52.
Synthesis of isoacteoside, a dihydroxyphenylethyl glycoside 总被引:1,自引:0,他引:1
Toshinari Kawada Ryuji Asano Kozo Makino Tomoyasu Sakuno 《Journal of Wood Science》2002,48(6):512-515
The total chemical synthesis of isoacteoside (1), 2-(3,4-dihydroxyphenyl)ethyl 6-O-caffeoyl-3-O-(-l-rhamnopyranosyl)--d-glucopyranoside, is described. An acteoside acetate with benzyl groups at the catechols (3: 2-(3,4-dibenzyloxyphenyl)ethyl 2,6-di-O-acetyl-4-O-[3,4-bis(O-benzyl)caffeoyl]-3-O-(-l-rhamnopyranosyl)--d-glucopyranoside) was treated with a solution of methy-lamine in methanol (MeNH2 in MeOH) to perform both deacetylation and caffeoyl migration, affording an isoacteoside derivative with benzyl groups at the catechols4b: 2-(3,4-dibenzyloxyphenyl)ethyl 6-O-[3,4-bis(O-benzyl) caffeoyl] -3-O-(-l-rhamnopyranosyl)--d-glucopyranoside —in 34% yield. Debenzylation of4b was successfully accomplished by catalytic transfer hydrogenation using 1,4-cyclohexadiene to give the target compound isoacteoside (1) in 54% yield.1H and13C nuclear magnetic resonance spectral data of the synthesized isoacteoside (1) were identical with those of the natural isoacteoside isolated fromPaulownia tomentosa (Thumb.) Steud.Part of this research was presented at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001 相似文献
53.
Daiki KATO Mariko OISHI Koichi OHNO Ko NAKASHIMA Atsuhito WADA Tatsushi MORITA Soichi IMAI Masaya TSUBOI James K. CHAMBERS Kazuyuki UCHIDA Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1499-1502
Ollulanus tricuspis is a small nematode parasite of the
stomach, and its infection has been reported worldwide in cats but only one report in dogs
as post-mortem diagnosis. Two dogs, kept in the Tokyo area, were presented for chronic
vomiting. Chronic gastritis was diagnosed histologically, and many nematodes were detected
in endoscopically-biopsied gastric samples and in the mucus of vomitus in both dogs. The
parasites were small (<1 mm), and their morphological characteristics were consistent
with those previously reported for O. tricuspis. The symptoms in one dog
completely disappeared after anthelmintic therapy. To our knowledge, this is the first
report describing ante-mortem diagnosis of spontaneous gastric O.
tricuspis infection in dogs in which infectivity and pathogenicity of the
nematode are suggested. 相似文献
54.
Kenji TSUKANO Kazuyuki SUZUKI Toshio SHIMAMORI Ayano SATO Katsunori KUDO Ryuji ASANO Tadaharu AJITO Jeffrey LAKRITZ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):67-73
The aim of the present study was to investigate the relationships between serum
amino acid profiles in normal and calves with Mycoplasma
bronchopneumonia. Serum free amino acid concentrations in serum obtained from 34 calves
with or without Mycoplasma bronchopneumonia were determined by
high-performance liquid chromatography. The calves with Mycoplasma were
characterized by significantly lower total amino acid and total essential amino acid
concentrations and molar ratios of branched-chain amino acid (BCAA) to aromatic amino acid
(BCAA/AAA) and BCAA to tyrosine (BTR), and by a significantly higher molar ratio of serine
phosphorylation (SPR). The proposed diagnostic cutoffs for BCAA/AAA, BTR and SPR in serum
based on ROC analysis for detection of catabolic states associated with
Mycoplasma bronchopneumonia were set at <1.75, <2.86 and
>0.85, respectively. Our results suggest that determining the profiles of amino acids,
especially BTR and SPR, could provide useful diagnostic information in terms of predicting
protein catabolism in Mycoplasma bronchopneumonia. 相似文献
55.
Yousuke MURATA James Kenn CHAMBERS Kazuyuki UCHIDA Ko NAKASHIMA Yasuko HANAFUSA Mitsutaka IKEZAWA Takashi SUGITA Hiroyuki NAKAYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1285-1288
A 10-year-old castrated male mixed-breed dog exhibited vomiting, lethargy and anorexia.
An abdominal ultrasound examination revealed a focal dilation of the abdominal aorta. The
dog died 2 days after examination, and necropsy revealed a ruptured aneurysm at the
abdominal aorta between the kidneys. Histological examination revealed severe
granulomatous and necrotizing pan-arteritis with fungal organisms.
Graphium species was identified through DNA sequence analysis of the
PCR product from formalin-fixed paraffin-embedded samples. To our knowledge, this is the
first report of aortic aneurysm caused by Graphium species in a dog. 相似文献
56.
A total of 772 canine fecal specimens were collected in Tochigi in 1979, 1991 and 2001. The frequency of dogs that were kept indoor in 2002 (72.8%) was significantly higher than that for 1979 (21.2%, P < 0.001) and 1991 (40.6%, P < 0.01). The frequency of parasitized dogs in 2002 (18.8%) was significantly lower than that for 1979 (41.9%, P < 0.01). The findings of this study confirm the declining trend in Japan in the prevalence of helminth parasites in dogs, particularly Ancylostoma caninum and Trichuris vulpis. The reduction in the frequency of dogs with helminths may be mainly a result of the improvement in the environment and the routine use of filaricides. 相似文献
57.
Changes of leukocyte counts and expression of pro‐ and anti‐inflammatory cytokines in peripheral leukocytes in periparturient dairy cows with retained fetal membranes 下载免费PDF全文
Takashi Shimizu Ikumi Morino Ryuji Kitaoka Akio Miyamoto Chiho Kawashima Shingo Haneda Fumie Magata 《Animal Science Journal》2018,89(9):1371-1378
In dairy cows, retained fetal membranes (RFM) affect reproductive performance. The aim of this study was to examine the leukocyte counts and the gene expression of tumour necrosis factor α (TNFα), interleukin 1β (IL‐1β), IL‐8, and IL‐10 in polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) in cows with (n = 5) or without (n = 5) RFM during the peripartum period. The lymphocyte counts in RFM cows were higher than those in control cows throughout the experiment (p < .05). The expression of IL‐8 in PMNs of control cows was higher (p < .05) compared with that of RFM cows postpartum. In cows with RFM, IL‐1β expression was higher (p < .05) in PMNs at 6 weeks postpartum whereas the expression of IL‐1β was lower (p < .05) in PBMCs at 4 weeks postpartum. The expression of IL‐10 in PBMCs of control cows was higher (p < .05) than that of RFM cows at 2 weeks prepartum and 4 weeks postpartum. Taken together, our data indicate that changes of gene expression of pro‐ and anti‐inflammatory cytokines in RFM cows might be associated with the delayed placental separation and development of uterine inflammation in RFM cows. 相似文献
58.
Takashi Kitagawa Takashi Nakagawa Ryuji Kimura Hiroshi Niino Shingo Kimura 《Fisheries Science》2011,77(2):217-222
Arabesque greenling Pleurogrammus azonus form schools of 30,000–60,000 individuals to feed on mesozooplankton near the sea surface. The fish need to be close to the
surface where mesozooplankton production occurs, but the food is not sufficiently dense there for the fish to grow optimally
while avoiding predation by sea birds. Arabesque greenling use a unique method to optimize their feeding conditions while
avoiding staying on the sea surface. When the school swims upward, water is pushed downward. Upward swimming of a school of
30,000 individuals generates a downward stream of about 0.8–1.1 m/s, resulting in a convergent flow near the surface. This
convergence concentrates the mesozooplankton on the sea surface and transports them into deeper layers with a strong vortex
about 3.0 m in diameter and 10–20 m long. Thus, schooling of this fish induces vortex flows that provide a rich feeding environment. 相似文献
59.
Masafumi SHIMIZU Yoshiko NAKAGAWA Yukio SATO Tamotsu FURUMAI Yasuhiro IGARASHI Hiroyasu ONAKA Ryuji YOSHIDA Hitoshi KUNOH 《Journal of General Plant Pathology》2000,66(4):360-366
To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of rhododendron, young plants of
rhododendron were surface-sterilized for use as an isolation source. Nine, six and two isolates, with distinguishing characteristics
based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves, respectively, suggesting that
various species of actinomycetes grow in the respective organs of this plant as symbionts or parasites. On an agar medium,
only isolate R-5 commonly formed a clear growth-inhibition zone against two major fungal pathogens of rhododendron, Phytophthora cinnamomi and Pestalotiopsis sydowiana, indicating that this isolate can produce antifungal material(s). Acetone extracts of a liquid culture of R-5 had a broad antimicrobial
spectrum against Gram-positive bacteria, yeast and filamentous fungi. Isolate R-5 was identified as a Streptomyces sp. based on morphological, physiological and chemotaxonomical characteristics. The present results indicate that isolate
R-5 is a suitable candidate for the biocontrol of diseases of rhododendron.
Received 25 March 2000/ Accepted in revised form 18 May 2000 相似文献
60.
大豆有效营养面积研究进展 总被引:1,自引:0,他引:1
2001~2004年,运用作物有效营养面积理论,对北方垄作区大豆垄幅的合理性问题进行了试验研究。研究表明大豆主产区黑龙江省中部、南部地区大豆有效营养面积为2210~4500 cm2,有效株行距为47.0~67.1 cm。其中中部地区的有效营养面积在2210~4025 cm2,即有效株行距为47.0~63.0 cm,南部地区的有效营养面积在3838~4500 cm2,即有效株行距为61.9~67.1 cm。南部地区品种有效营养面积较大,中部地区品种有效营养面积较小。据此阐明目前生产上广泛采用67~70 cm的垄作,已超过了大豆的有效株行距,造成了土壤资源等的浪费。尤其是中、北部垄作区采用55 cm左右的垄距种植大豆,将会更有利于大豆群体产量的提高。该理论为目前黑龙江省北部地区栽培面积逐渐增大的大豆窄行垄栽培提供了有力的理论根据。 相似文献