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排序方式: 共有609条查询结果,搜索用时 31 毫秒
81.
Nicolas Marron Cécilia Gana Dominique Gérant Pascale Maillard Pierrick Priault Daniel Epron 《植物养料与土壤学杂志》2018,181(2):296-304
Estimating symbiotic di‐nitrogen (N2) fixation is challenging, especially when working with woody N2 fixers in field trials. Fortunately, isotope methods based on 15N natural abundance or on 15N artificial enrichment (dilution method) make it possible to estimate the proportion of nitrogen derived from the atmosphere (Ndfa) in N2‐fixing species. These methods have been extensively used in the field for herbaceous species, much less for tree species such as alder and acacia, and rarely for black locust (Robinia pseudoacacia). The objectives of this study were to characterize the fixation potential of black locust in a plantation by using the two 15N isotope methods in the field, and to document values of isotope fractionation occurring during N2 fixation (the B value). B values were estimated both by growing trees on an N‐free medium in controlled conditions (Blab) and by making Ndfa calculated with the natural abundance method converge with Ndfa calculated with the 15N dilution method in the field (Bfield). The two methods gave consistent estimates of the B value. B values ranging between –1.4 and –3.2‰ were found, varying with the age of the plant material. Up to 76% of the N in the black locust trees came from the atmosphere, representing more than 45 kg N ha?1 over five years and confirming that black locust may be well adapted to N‐poor soils. 相似文献
82.
M. A. H. B. Bhuiyan T. Groom M. E. Nicolas P. W. J. Taylor 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(4):1041-1048
Pyrethrum seed has an important role in the transmission of Stagonosporopsis tanaceti, the cause of ray blight disease of pyrethrum. A TaqMan probe based polymerase chain reaction (PCR) assay was developed to quantify the level of S. tanaceti inocula in pyrethrum seed and seedlings. Primer pair (St_qF3, St_qR2) was designed based on the intergenic spacer (IGS) region of S. tanaceti, which produced a 125 bp amplicon specific to S. tanaceti. TaqMan PCR assay using St_qF3, St_qR2 and a probe St_qP was highly specific against the genomic DNA of S. tanaceti, but did not amplify DNA of 14 related Stagonosporopsis species or other foliar pathogens of pyrethrum. The sensitivity limit of this assay was measured using the cycle threshold (Ct) value which ranged from 17.59 for 10 nanograms (ng) to 36.34 for 100 femtograms (fg) genomic DNA of S. tanaceti. There was a significant negative correlation (r = ?0.999, P < 0.001) between the Ct value and the percent of S. tanaceti infected seed. In addition, this TaqMan PCR assay detected latent infection within seedlings. This assay could be applied to test commercial seed and seedlings before deciding on the appropriate management practices. 相似文献
83.
Nicolas Marinval Pierre Saboural Oualid Haddad Murielle Maire Kevin Bassand Frederic Geinguenaud Nadia Djaker Khadija Ben Akrout Marc Lamy de la Chapelle Romain Robert Olivier Oudar Erwan Guyot Christelle Laguillier-Morizot Angela Sutton Cedric Chauvierre Frederic Chaubet Nathalie Charnaux Hanna Hlawaty 《Marine drugs》2016,14(10)
Herein we investigate the structure/function relationships of fucoidans from Ascophyllum nodosum to analyze their pro-angiogenic effect and cellular uptake in native and glycosaminoglycan-free (GAG-free) human endothelial cells (HUVECs). Fucoidans are marine sulfated polysaccharides, which act as glycosaminoglycans mimetics. We hypothesized that the size and sulfation rate of fucoidans influence their ability to induce pro-angiogenic processes independently of GAGs. We collected two fractions of fucoidans, Low and Medium Molecular Weight Fucoidan (LMWF and MMWF, respectively) by size exclusion chromatography and characterized their composition (sulfate, fucose and uronic acid) by colorimetric measurement and Raman and FT-IR spectroscopy. The high affinities of fractionated fucoidans to heparin binding proteins were confirmed by Surface Plasmon Resonance. We evidenced that LMWF has a higher pro-angiogenic (2D-angiogenesis on Matrigel) and pro-migratory (Boyden chamber) potential on HUVECs, compared to MMWF. Interestingly, in a GAG-free HUVECs model, LMWF kept a pro-angiogenic potential. Finally, to evaluate the association of LMWF-induced biological effects and its cellular uptake, we analyzed by confocal microscopy the GAGs involvement in the internalization of a fluorescent LMWF. The fluorescent LMWF was mainly internalized through HUVEC clathrin-dependent endocytosis in which GAGs were partially involved. In conclusion, a better characterization of the relationships between the fucoidan structure and its pro-angiogenic potential in GAG-free endothelial cells was required to identify an adapted fucoidan to enhance vascular repair in ischemia. 相似文献
84.
Pauline Kamermans Ainhoa Blanco Sandra Joaquim Domitília Matias Thorolf Magnesen Jean Louis Nicolas Bruno Petton Rene Robert 《Aquaculture International》2016,24(3):827-842
In order to increase production of bivalves in hatcheries and nurseries, the development of new technology and its integration into commercial bivalve hatcheries is important. Recirculation aquaculture systems (RASs) have several advantages: high densities of the species can be cultured resulting in a cost-effective production system; optimal temperature maximizes production and allows rapid turnover of the product; stable water quality improves growth rate and minimizes stress and potential loss by diseases. Pilot RAS systems were developed for seed rearing of oysters (Crassostrea gigas), scallops (Pecten maximus), mussels (Mytilus edulis) and clams (Ruditapes decussatus). Optimal feed addition and waste matrix were determined. Based on this, system flow rates were designed. Seed growth in the pilot RAS systems was compared at different renewal rates and with growth in flow-through systems (FTS). All four species can be reared in RAS and showed similar growth in RAS and in FTS or in RAS with a higher renewal rate. RAS can keep O2, nitrogen and pH within the desired range. Temperature was generally higher in RAS than in FTS, probably due to heat induced by the pump circulating the water. The supply of sufficient amount of food in combination with a desire to reduce the renewal rate calls for use of concentrated feed in RAS. 相似文献
85.
Intraguild predation of Geocoris punctipes on Eretmocerus eremicus and its influence on the control of the whitefly Trialeurodes vaporariorum 下载免费PDF全文
86.
87.
<正>Manipulating the gastrointestinal microbial ecosystem to enhance animal performance and reproductive responses has been one of the main goals of animal science researchers and veterinarians.Recent restrictions to the use of antimicrobials as growth promoters led researchers to seek alternative practices that can show promise both from the standpoint of efficacy as well as from the practical and economic aspects. 相似文献
88.
Oualid Haddad Erwan Guyot Nicolas Marinval Fabien Chevalier Lo?c Maillard Latifa Gadi Christelle Laguillier-Morizot Olivier Oudar Angela Sutton Nathalie Charnaux Hanna Hlawaty 《Marine drugs》2015,13(11):6588-6608
Induction of angiogenesis is a potential treatment for chronic ischemia. Low molecular weight fucoidan (LMWF), the sulfated polysaccharide from brown seaweeds, has been shown to promote revascularization in a rat limb ischemia, increasing angiogenesis in vivo. We investigated the potential role of two heparan sulfate (HS) metabolism enzymes, exostosin-2 (EXT2) and heparanase (HPSE), and of two HS-membrane proteoglycans, syndecan-1 and -4 (SDC-1 and SDC-4), in LMWF induced angiogenesis. Our results showed that LMWF increases human vascular endothelial cell (HUVEC) migration and angiogenesis in vitro. We report that the expression and activity of the HS-degrading HPSE was increased after LMWF treatment. The phenotypic tests of LMWF-treated and EXT2- or HPSE-siRNA-transfected cells indicated that EXT2 or HPSE expression significantly affect the proangiogenic potential of LMWF. In addition, LMWF increased SDC-1, but decreased SDC-4 expressions. The effect of LMWF depends on SDC-4 expression. Silencing EXT2 or HPSE leads to an increased expression of SDC-4, providing the evidence that EXT2 and HPSE regulate the SDC-4 expression. Altogether, these data indicate that EXT2, HPSE, and SDC-4 are involved in the proangiogenic effects of LMWF, suggesting that the HS metabolism changes linked to LMWF-induced angiogenesis offer the opportunity for new therapeutic strategies of ischemic diseases. 相似文献
89.
Christelle Simon-Colin Yannick Gueguen Evelyne Bachere Achraf Kouzayha Denis Saulnier Nicolas Gayet Jean Guezennec 《Marine drugs》2015,13(6):3732-3744
Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with two marine bacterial exopolymers as alternative treatment agents. In field studies, the combination treatment resulted in a significant reduction in graft failures vs. untreated controls. The combination of tachyplesin (73 mg/L) with two bacterial exopolysaccharides (0.5% w/w) acting as filming agents, reduces graft-associated bacterial contamination. The survival data were similar to that reported for antibiotic treatments. These data suggest that non-antibiotic treatments of pearl oysters may provide an effective means of improving oyster survival following grafting procedures. 相似文献
90.
Eva van Gorsel Nicolas Delpierre Ray Leuning Andy Black J. William Munger Steven Wofsy Marc Aubinet Christian Feigenwinter Jason Beringer Damien Bonal Baozhang Chen Jiquan Chen Robert Clement Kenneth J. Davis Ankur R. Desai Danilo Dragoni Sophia Etzold Thomas Grünwald Lianhong Gu Bernhard Heinesch Lucy R. Hutyra Wilma W.P. Jans Werner Kutsch B.E. Law Monique Y. Leclerc Ivan Mammarella Leonardo Montagnani Asko Noormets Corinna Rebmann Sonia Wharton 《Agricultural and Forest Meteorology》2009,149(11):1919
Micrometeorological measurements of nighttime ecosystem respiration can be systematically biased when stable atmospheric conditions lead to drainage flows associated with decoupling of air flow above and within plant canopies. The associated horizontal and vertical advective fluxes cannot be measured using instrumentation on the single towers typically used at micrometeorological sites. A common approach to minimize bias is to use a threshold in friction velocity, u*, to exclude periods when advection is assumed to be important, but this is problematic in situations when in-canopy flows are decoupled from the flow above. Using data from 25 flux stations in a wide variety of forest ecosystems globally, we examine the generality of a novel approach to estimating nocturnal respiration developed by van Gorsel et al. (van Gorsel, E., Leuning, R., Cleugh, H.A., Keith, H., Suni, T., 2007. Nocturnal carbon efflux: reconciliation of eddy covariance and chamber measurements using an alternative to the u*-threshold filtering technique. Tellus 59B, 397–403, Tellus, 59B, 307-403). The approach is based on the assumption that advection is small relative to the vertical turbulent flux (FC) and change in storage (FS) of CO2 in the few hours after sundown. The sum of FC and FS reach a maximum during this period which is used to derive a temperature response function for ecosystem respiration. Measured hourly soil temperatures are then used with this function to estimate respiration RRmax. The new approach yielded excellent agreement with (1) independent measurements using respiration chambers, (2) with estimates using ecosystem light-response curves of Fc + Fs extrapolated to zero light, RLRC, and (3) with a detailed process-based forest ecosystem model, Rcast. At most sites respiration rates estimated using the u*-filter, Rust, were smaller than RRmax and RLRC. Agreement of our approach with independent measurements indicates that RRmax provides an excellent estimate of nighttime ecosystem respiration. 相似文献