不同相思树种根瘤菌理化特征研究

通过牛肉膏蛋白胨试验、柠檬酸盐利用试验、碳源利用试验、3-酮基乳糖试验、B.T. B反应试验及明胶水解试验等角度分析漳州中西国有林场6种相思树种根瘤茵生理及生化特征.研究结果表明:从生理特征上看.在牛肉膏蛋白胨试验中所有供试菌株都生长不良;所有供试菌株都能利用柠檬酸盐;灰木相思的碳源利用能力最强,而厚荚相思与马占相思碳源利用能力比较弱;马占相思及灰木相思可较充分利用木糖,卷荚相思、纹荚相思、薄荚相思可较充分利用半乳糖;从生化特性上看,除马占相思根瘤茵在YMA培养基上产碱外,其余相思树种菌株均产酸;卷荚相思及纹荚相思根瘤茵能产生蛋白分解酶,而其余相思树种根瘤茵则不能产生蛋白分解酶.     

镉胁迫对青菜幼苗某些生理特性以及基因组多态性的影响

以青菜品种"苏州青"为试材,采用生理生化方法和随机扩增多态性DNA(RAPD)技术,研究了镉(Cd)胁迫对青菜幼苗相关生理特性以及基因组多态性的影响。结果表明:不同浓度(1、5、10、20、40 mg·L-1)的Cd处理20 d后,青菜幼苗的鲜重、根伸长受到显著抑制,叶片的叶绿素以及蛋白质含量下降,丙二醛含量和细胞膜透性增加。选用8条寡核苷酸引物(10 bp)对青菜幼苗基因组DNA进行RAPD扩增,对照组的RAPD图谱中可分辨出55条谱带,Cd胁迫后的RAPD图谱发生改变,呈现谱带的增加、缺失和荧光强度的改变,并且与Cd浓度剂量-效应相关。随着Cd浓度的增加,幼苗叶片基因组的模板稳定性逐步下降,并且RAPD多态性与青菜幼苗的生理指标具有显著的相关性。以上结果表明,利用RAPD技术获得的DNA多态性变化谱带可作为检测Cd对青菜毒性效应的生物标记物。     

Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant

Cytosolic calcium oscillations control signaling in animal cells, whereas in plants their importance remains largely unknown. In wild-type Arabidopsis guard cells abscisic acid, oxidative stress, cold, and external calcium elicited cytosolic calcium oscillations of differing amplitudes and frequencies and induced stomatal closure. In guard cells of the V-ATPase mutant det3, external calcium and oxidative stress elicited prolonged calcium increases, which did not oscillate, and stomatal closure was abolished. Conversely, cold and abscisic acid elicited calcium oscillations in det3, and stomatal closure occurred normally. Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure.     

基于神经网络的电控发动机怠速不稳定的故障识别方法

以电控发动机怠速不稳定这种十分常见的故障为研究对象。系统地介绍了应用神经网络进行故障原因识别的方法。详细地分析了不同网络结构的训练效率和以不同样本形式训练的网络对相同故障症兆的故障原因的识别能力。     

Observation of polymer conformation hysteresis in extensional flow

Highly extensible Escherichia coli DNA molecules in planar extensional flow were visualized in dilute solution by fluorescence microscopy. For a narrow range of flow strengths, the molecules were found in either a coiled or highly extended conformation, depending on the deformation history of the polymer. This conformation hysteresis persists for many polymer relaxation times and is due to conformation-dependent hydrodynamic forces. Polymer conformational free-energy landscapes were calculated from computer simulations and show two free-energy minima for flow strengths near the coil-stretch transition. Hysteresis cycles may directly influence bulk-solution stresses and the development of stress-strain relations for dilute polymer flows.     

野柿A砧与几个甜柿品种嫁接早期亲和力观察

用3个不同甜柿（Diospyros kaki)品种与野柿（Diospyros kaki var.silvestris)A砧进行嫁接亲和力对比试验。结果表明：禅寺丸麻城甜柿与野柿A砧亲和性良好，定植二年生野柿A砧后嫁接建园，接后第1年始花，第2年结果，前川次郎与野柿A砧嫁接存在明显不亲和性，少量植株嫁接当年接口呈现大、小脚现象、次年枝条生长细弱，第3年植株萎缩。     

Dispersed polaron simulations of electron transfer in photosynthetic reaction centers

A microscopic method for simulating quantum mechanical, nuclear tunneling effects in biological electron transfer reactions is presented and applied to several electron transfer steps in photosynthetic bacterial reaction centers. In this "dispersed polaron" method the fluctuations of the protein and the electron carriers are projected as effective normal modes onto an appropriate reaction coordinate and used to evaluate the quantum mechanical rate constant. The simulations, based on the crystallographic structure of the reaction center from Rhodopseudomonas viridis, focus on electron transfer from a bacteriopheophytin to a quinone and the subsequent back-reaction. The rates of both of these reactions are almost independent of temperature or even increase with decreasing temperature. The simulations reproduce this unusual temperature dependence in a qualitative way, without the use of adjustable parameters for the protein's Franck-Condon factors. The observed dependence of the back-reaction on the free energy of the reaction also is reproduced, including the special behavior in the "inverted region."     

农业增长方式转变与农业投资问题研究

论述了农业投资在推动农业增长方式的转变中的特殊地位和作用，以及农业投资规模，数量，结构和方向等对农业持续稳定增长的影响，并在此基础提出应确立有效的农业投资机制和正确的农业投资方向，对投资机制的确立和投资方向的调整提出了操作性较强的对策措施。     

Spherical bilayer vesicles of fullerene-based surfactants in water: a laser light scattering study

The low solubility of fullerenes in aqueous solution limits their applications in biology. By appropriate substitution, the fullerenes can be transformed into stabilized anions that are water soluble and can form large aggregated structures. A laser light scattering study of the association behavior of the potassium salt of pentaphenyl fullerene (Ph5C60K) in water revealed that the hydrocarbon anions Ph5C60- associate into bilayers, forming stable spherical vesicles with an average hydrodynamic radius and a radius of gyration of about 17 nanometers at a very low critical aggregation concentration of less than 10(-7) moles per liter. The average aggregation number of associated particles in these large spherical vesicles is about 1.2 x 10(4).     

云杉小黑天牛幼虫空间分布型的研究

1988年在大兴安岭火烧迹地上,调查了云杉小黑天牛(Monochamus sutor L)幼虫的空间分布。采用扩散系数(C),Iwao的m—m回归分析如Taylor幂的法则等7种方法,测定云杉小黑天牛幼虫的空间分布型呈聚集分布,分布的基本成分是个体群。扩散蔓延规律为聚集型扩散。其聚集程度随火烧程度、有虫株率及虫口密度大于5～7个侵入孔/1000 cm~2时种群趋于随机或均匀分布。聚集原因分析表明,平均1000 cm~2树干面积上2.13头幼虫是该虫种群聚集机制发生变化的临界值。