排序方式: 共有64条查询结果,搜索用时 15 毫秒
21.
Dahan M Lévi S Luccardini C Rostaing P Riveau B Triller A 《Science (New York, N.Y.)》2003,302(5644):442-445
Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors. 相似文献
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Krzysztof Rakus Ping Ouyang Maxime Boutier Maygane Ronsmans Anca Reschner Catherine Vancsok Joanna Jazowiecka-Rakus Alain Vanderplasschen 《Veterinary research》2013,44(1):85
Cyprinid herpesvirus 3 (CyHV-3), a member of the family Alloherpesviridae is the causative agent of a lethal, highly contagious and notifiable disease in common and koi carp. The economic importance of common and koi carp industries together with the rapid spread of CyHV-3 worldwide, explain why this virus became soon after its isolation in the 1990s a subject of applied research. In addition to its economic importance, an increasing number of fundamental studies demonstrated that CyHV-3 is an original and interesting subject for fundamental research. In this review, we summarized recent advances in CyHV-3 research with a special interest for studies related to host-virus interactions. 相似文献
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Angaman DM Petrizzo R Hernández-Gras F Romero-Segura C Pateraki I Busquets M Boronat A 《Plant methods》2012,8(1):1
Background
Carotenoids are the most widespread group of pigments found in nature. In addition to their role in the physiology of the plant, carotenoids also have nutritional relevance as their incorporation in the human diet provides health benefits. In non-photosynthetic tissues, carotenoids are synthesized and stored in specialized plastids called chromoplasts. At present very little is known about the origin of the metabolic precursors and cofactors required to sustain the high rate of carotenoid biosynthesis in these plastids. Recent proteomic data have revealed a number of biochemical and metabolic processes potentially operating in fruit chromoplasts. However, considering that chloroplast to chromoplast differentiation is a very rapid process during fruit ripening, there is the possibility that some of the proteins identified in the proteomic analysis could represent remnants no longer having a functional role in chromoplasts. Therefore, experimental validation is necessary to prove whether these predicted processes are actually operative in chromoplasts. 相似文献25.
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Maxime Olmos Flix Massiot‐Granier Etienne Prvost Grald Chaput Ian R Bradbury Marie Nevoux Etienne Rivot 《Fish and Fisheries》2019,20(2):322-342
A hierarchical Bayesian life cycle model is presented that considers spatial covariation of marine life history traits of Atlantic salmon (Salmo salar) populations in the North Atlantic. The model is based on a collective analysis of the dynamics of 13 stock units (SUs) from two continental stock groups (CSGs) in North America and Southern Europe in a single hierarchical model over the period 1971–2014. The model sets up a new assessment framework for Atlantic salmon stocks. It also provides a framework to investigate the drivers of changes in Atlantic salmon population dynamics including disentangling the effects of fisheries from those of environmental factors in a hierarchy of spatial scales. It is used to test the hypothesis of a strong spatial synchrony in marine life history dynamics of Atlantic salmon populations. The trends in two key parameters associated with the early marine phase of the life cycle are estimated: (i) the marine survival during the first summer–autumn spent at sea and (ii) the proportion of fish maturing after the first winter at sea. The results provide evidence of a decline in the marine survival together with an increase in the proportion of fish that mature after the first winter at sea, common to all SUs. Our results show an increased coherence in the covariations of trends in these two marine life history traits related to geographic proximity of SUs which support the hypothesis of a coherent response of geographically proximate Atlantic salmon populations that likely share similar migration routes. 相似文献
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Jessica Girona Josée-Anne Lévesque Maxime Paré Mireille Bellemare 《International Journal of Fruit Science》2019,19(2):165-178
Wind exposure may reduce the snowpack and increase frost damage to lowbush blueberry (Vaccinium angustifolium Ait.). Farmers in the Saguenay-Lac-Saint-Jean region (Québec) have therefore installed natural windbreaks to maintain a deep snow cover. We verified the efficiency of 39 windbreaks distributed over two farms. Over two consecutive years, snow depth was measured along transects established perpendicular to these windbreaks. In the first summer, frost damage and yields were assessed at each point where snow depth had been measured. We found large fluctuations in snow depth along most transects, with deeper snow occurring within 20 m on either side of windbreaks. Relative snow depth distribution was correlated between years on 19 transects. On one farm, one third of total transect length was below the recognized critical depth of 30 cm. On this farm, the proportion of damaged stems, the mean length of stem damage and the damaged-to-total stem length decreased exponentially with increasing snow depth. Likewise, the number of fruits per stem decreased exponentially with the length of stem damage and increased linearly with snow depth. Given current trends in climate change, our results serve as a timely call for improving winter windbreak design in the Saguenay-Lac-Saint-Jean region. 相似文献
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Fournier G Boutier M Stalin Raj V Mast J Parmentier E Vanderwalle P Peeters D Lieffrig F Farnir F Gillet L Vanderplasschen A 《Veterinary research》2012,43(1):6
ABSTRACT: Cyprinid herpesvirus 3 (CyHV-3), also known as Koi herpesvirus, is the etiological agent of a mortal disease in common and koi carp. Recently, we investigated the entry of CyHV-3 in carp using bioluminescence imaging and a CyHV-3 recombinant strain expressing luciferase (LUC). We demonstrated that the skin is the major portal of entry after inoculation of carp by immersion in water containing CyHV-3. While this model of infection mimics some natural conditions in which infection takes place, other epidemiological conditions could favour entry of virus through the digestive tract. Here, we investigated whether ingestion of infectious materials mediates CyHV-3 entry through the digestive tract. Carp were fed with materials contaminated with the CyHV-3 LUC recombinant (oral contamination) or immersed in water containing the virus (contamination by immersion). Bioluminescence imaging analyses performed at different times post-infection led to the following observations: (i) the pharyngeal periodontal mucosa is the major portal of entry after oral contamination, while the skin is the major portal of entry after contamination by immersion. (ii) Both modes of inoculation led to the spreading of the infection to the various organs tested. However, the timing and the sequence in which some of the organs turned positive were different between the two modes of inoculation. Finally, we compared the disease induced by the two inoculation modes. They led to comparable clinical signs and mortality rate. The results of the present study suggest that, based on epidemiological conditions, CyHV-3 can enter carp either by skin or periodontal pharyngeal mucosal infection. 相似文献
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Alain Doucet Martin Williams Mylene C Gagnon Maxime Sasseville Marc Beauregard 《Journal of agricultural and food chemistry》2002,50(1):92-98
Protein design is currently used for the creation of new proteins with desirable traits. In this laboratory the focus has been on the synthesis of proteins with high essential amino acid content having potential applications in animal nutrition. One of the limitations faced in this endeavor is achieving stable proteins despite a highly biased amino acid content. Reported here are the synthesis and characterization of two disulfide-bridged mutants derived from the MB-1 designer protein. Both mutants outperformed their parent protein MB-1 with their bridge formed, as shown by circular dichroism, size exclusion chromatography, thermal denaturation, and proteolytic degradation experiments. When the disulfide bridges were cleaved, the mutants' behavior changed: the mutants significantly unfolded, suggesting that the introduction of Cys residues was deleterious to MB-1-folding. In an attempt to compensate for the mutations used, a Tyr62-Trp mutation was performed, leading to an increase in bulk and hydrophobicity in the core. The Trp-containing disulfide-bridged mutants did not behave as well as the original MB-1Trp, suggesting that position 62 might not be adequate for a compensatory mutation. 相似文献