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Pugliese Massimo Monchiero Matteo Gullino Maria Lodovica Garibaldi Angelo 《植物病害和植物保护杂志》2018,125(5):477-482
Journal of Plant Diseases and Protection - Erysiphe necator is favourably influenced by the increase in temperatures and remains the main pathogen threatening grape in Northern Italy. In the... 相似文献
203.
Cabrera-Chávez F Iametti S Miriani M de la Barca AM Mamone G Bonomi F 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):24-30
Maize is used as an alternative to wheat to elaborate foodstuffs for celiac patients in a gluten-free diet. However, some
maize prolamins (zeins) contain amino acid sequences that resemble the wheat gluten immunodominant peptides and their integrity
after gastrointestinal proteolysis is unknown. In this study, the celiac IgA-immunoreactivity to zeins from raw or nixtamalized
grains, before and after peptic/tryptic digestion was evaluated and their possible immunogenicity was investigated by in silico methods. IgA from some celiac patients with HLA-DQ2 or DQ8 haplotypes recognized two alpha-zeins even after peptic/tryptic
proteolysis. However, digestion affected zeins after denaturation, reduction, and alkylation, used for identification of prolamins
as alpha-zein A20 and A30 by MS/MS sequencing. An in silico analysis indicated that other zeins contain similar sequences, or sequences that may bind even better to the HLA-DQ2/DQ8
molecules compared to the already identified ones. Results concur to indicate that relative abundance of these zeins, along
with factors affecting their resistance to proteolysis, may be of paramount clinical relevance, and the use of maize in the
formulation and preparation of gluten-free foods must be reevaluated in some cases of celiac disease. 相似文献