全文获取类型
收费全文 | 2927篇 |
免费 | 157篇 |
专业分类
林业 | 246篇 |
农学 | 113篇 |
基础科学 | 10篇 |
817篇 | |
综合类 | 142篇 |
农作物 | 179篇 |
水产渔业 | 247篇 |
畜牧兽医 | 964篇 |
园艺 | 56篇 |
植物保护 | 310篇 |
出版年
2024年 | 3篇 |
2023年 | 41篇 |
2022年 | 71篇 |
2021年 | 115篇 |
2020年 | 121篇 |
2019年 | 112篇 |
2018年 | 116篇 |
2017年 | 110篇 |
2016年 | 118篇 |
2015年 | 85篇 |
2014年 | 121篇 |
2013年 | 200篇 |
2012年 | 190篇 |
2011年 | 275篇 |
2010年 | 146篇 |
2009年 | 128篇 |
2008年 | 193篇 |
2007年 | 177篇 |
2006年 | 146篇 |
2005年 | 121篇 |
2004年 | 116篇 |
2003年 | 94篇 |
2002年 | 93篇 |
2001年 | 20篇 |
2000年 | 31篇 |
1999年 | 19篇 |
1998年 | 7篇 |
1997年 | 16篇 |
1996年 | 12篇 |
1995年 | 3篇 |
1994年 | 9篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1984年 | 6篇 |
1982年 | 2篇 |
1981年 | 7篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1973年 | 1篇 |
1971年 | 2篇 |
1968年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有3084条查询结果,搜索用时 11 毫秒
101.
Alberto Falco Mikolaj Adamek Patricia Pereiro David Hoole Jos Antonio Encinar Beatriz Novoa Ricardo Mallavia 《Marine drugs》2022,20(6)
The high proliferation of microorganisms in aquatic environments has allowed their coevolution for billions of years with other living beings that also inhabit these niches. Among the different existing types of interaction, the eternal competition for supremacy between the susceptible species and their pathogens has selected, as part of the effector division of the immune system of the former ones, a vast and varied arsenal of efficient antimicrobial molecules, which is highly amplified by the broad biodiversity radiated, above any others, at the marine habitats. At present, the great recent scientific and technological advances already allow the massive discovery and exploitation of these defense compounds for therapeutic purposes against infectious diseases of our interest. Among them, antimicrobial peptides and antimicrobial metabolites stand out because of the wide dimensions of their structural diversities, mechanisms of action, and target pathogen ranges. This revision work contextualizes the research in this field and serves as a presentation and scope identification of the Special Issue from Marine Drugs journal “The Immune System of Marine Organisms as Source for Drugs against Infectious Diseases”. 相似文献
102.
Simulating in situ ammonia volatilization losses in the North China Plain using a dynamic soil‐crop model 下载免费PDF全文
Anna Michalczyk Kurt Christian Kersebaum Lisa Heimann Marco Roelcke Qin‐Ping Sun Xin‐Ping Chen Fu‐Suo Zhang 《植物养料与土壤学杂志》2016,179(2):270-285
Ammonia (NH3) volatilization is an important N loss pathway in intensive agriculture of the North China Plain (NCP). Simulation models can help to assess complex N and water processes of agricultural soil–crop systems. Four variations (Var) of a sub‐module for the deterministic, process‐based HERMES model were implemented ranging from simple empirical functions (Var 3 and 4) to process‐oriented approaches (Var 1 and 2) including the main processes of NH3 volatilization, urea hydrolysis, nitrification from ammonium‐based N fertilizer, and changes in soil solution pH. Ammonia volatilization, plant growth, and changes in ammonium and nitrate pools in the soil over several winter wheat–summer maize double‐crop rotations at three locations in the NCP were simulated. Results were calibrated with two data sets (Dongbeiwang 1, Shunyi) and validated using two data sets (Dongbeiwang 2, Quzhou). They showed that the ammonia volatilization sub‐module of the HERMES model worked well under the climatic and soil conditions of N China. Although the simpler equations, Var 3 and 4, showed lower deviations to observed volatilization across all sites and treatments with a mean absolute error (MAE) of 1.8 and 1.4 in % of applied N, respectively, compared to process‐oriented approaches, Var 1 and 2, with a MAE of 2.2 and 1.9 in % of applied N, respectively. Environmental conditions were reflected better by the process‐oriented approaches. Generally, simulation results were satisfying but simulated changes in topsoil pH need further verification with measurements. 相似文献
103.
Márcia Regina Fagundes-Klen Márcia Teresinha Veit Carlos Eduardo Borba Rosangela Bergamasco Luiz Gustavo de Lima Vaz Edson Antonio da Silva 《Water, air, and soil pollution》2010,213(1-4):15-26
Copper biosorption onto chemically modified biomass of marine alga Sargassum filipendula was investigated in a batch reactor and a fixed bed column. Experiments were carried out in the batch reactor to obtain kinetic and equilibrium data and to assess the copper desorption efficiency of different eluent solutions. The pseudo-first-order, pseudo-second-order, and Langmuir kinetic models were used to correlate kinetic data. The experimental data fitted well to the pseudo first order and Langmuir kinetic models. Langmuir and Freundlich models were applied to describe the equilibrium data obtained at a fixed temperature of 30°C and at pH values of 3.0, 4.0, 5.0, and 6.0. The maximum capacities of copper biosorption onto the algal biomass were 1.43, 1.59, 2.40, and 2.36 mequiv./g at pH 3.0, 4.0, 5.0, and 6.0, respectively. The efficiencies of two eluent solutions (calcium chloride and hydrochloric acid) for copper removal from the biomass were evaluated at different concentrations (0.1, 0.2, 0.5, and 1.0 mol/L). The efficiencies of the calcium chloride solutions varied from 1% to 14%, while efficiencies varying from 95% to 99% were obtained when hydrochloric acid solutions were applied. Three adsorption/desorption cycles were carried out in a fixed bed column using 0.1 mol/L hydrochloric acid as eluent solution. The results showed that an increase in the number of cycles led to a reduction in the adsorption capacity of the alga. The desorbed copper fraction presented no significant variation, remaining around 63% in the three adsorption/desorption cycles. 相似文献
104.
López-López A Jiménez-Araujo A García-García P Garrido-Fernández A 《Journal of agricultural and food chemistry》2007,55(26):10803-10811
Table olives constitute an important part of the Mediterranean diet and the diet of many non-olive-producing countries. The aim of this work was to determine the fiber, sugar, and organic acid contents in Spanish commercial presentations of table olives and characterize them by means of a multivariate analysis. The selection of variables was carried out on the basis of a canonical analysis and their classification, according to processing styles and cultivars, through a linear discriminant analysis. Values of dietary fiber in table olives ranged from 2 to 5 g/100 g edible portion (e.p.). Some stuffing materials (almond, hot red pepper, and hazelnut) or the addition of capers produced a significant increase in the total dietary fiber in green olives. Glucose, fructose, and mannitol were usually found in the ranges of 0-55, 0-70, and 0-107 mg/100 g e.p., respectively. Succinic acid was detected only in green and directly brined olives (0-40 mg/100 g e.p.), while lactic and acetic acids were used within the ranges of 0-681 and 5-492.8 mg/100 g e.p., respectively. A multivariate analysis showed that fiber, mannitol, and succinic, lactic, and acetic acids can be used to discriminate between processing styles (95.5% correct assignations) and cultivars (61.20%). Current data can also be used in the evaluation of the dietary value of table olives. 相似文献
105.
Venturi L Rocculi P Cavani C Placucci G Dalla Rosa M Cremonini MA 《Journal of agricultural and food chemistry》2007,55(26):10572-10578
Hydration of freeze-dried chicken breast meat was followed in the water activity range of aw=0.12-0.99 by a multianalytical approach comprising of sorption isotherm, differential scanning calorimetry (DSC), and nuclear magnetic resonance (NMR). The amount of frozen water and the shape of the T2-relaxogram were evaluated at each water content by DSC and NMR, respectively. Data revealed an agreement between sorption isotherm and DSC experiments about the onset of bulk water (aw=0.83-0.86), and NMR detected mobile water starting at aw=0.75. The origin of the short-transverse relaxation time part of the meat NMR signal was also reinvestigated through deuteration experiments and proposed to arise from protons belonging to plasticized matrix structures. It is proved both by D2O experiments and by gravimetry that the extra protons not contributing to the water content in the NMR experiments are about 6.4% of the total proton NMR CPMG signal of meat. 相似文献
106.
Chiara De Lucchi Piergiorgio Stevanato Linda Hanson Mitch McGrath Lee Panella Marco De Biaggi Chiara Broccanello Marco Bertaggia Luca Sella Giuseppe Concheri 《Euphytica》2017,213(3):71
Fusarium spp. cause severe damage in many agricultural crops, including sugar beet, with Fusarium oxysporum historically being considered as the most damaging of all species. Sugar beet needs to be protected from this class of soil-borne pathogens in order to ensure an optimal sugar yield in the field. Genetic control of the disease is crucial in managing these pathogens. Identification of single nucleotide polymorphism (SNP) markers linked to resistance can be a powerful tool for the introgression of valuable genes needed to develop Fusarium-resistant varieties. A candidate gene approach was carried out to identify SNP markers linked to putative Fusarium resistance sources in sugar beet. Five resistant analogue genes (RGAs) were screened by means of high resolution melting (HRM) analysis in a set of sugar beet lines, considered as resistant and susceptible to Fusarium oxysporum. HRM polymorphisms were observed in 80% of amplicons. Two HRM polymorphisms were significantly associated with Fusarium resistance (P < 0.05). The amplicons that showed association were sequenced and two SNPs were identified. The association was further validated on 96 susceptible and 96 resistant plants using competitive allele-specific PCR (KASPar) technology. The selected SNPs could be used for marker-assisted breeding of Fusarium resistance in sugar beet. 相似文献
107.
Henrique de Souza Luche José Antonio Gonzalez da Silva Rafael Nornberg Emilio Ghisleni Arenhardt Vanderlei da Rosa Caetano Luciano Carlos da Maia Antonio Costa de Oliveira 《Journal of Crop Science and Biotechnology》2017,20(2):73-80
In wheat, the increase of yield and stability associated traits can be achieved by combining parents containing the stay-green trait and favorable alleles for grain yield. The aim of this work was to analyze the genetic dissimilarity between wheat lines from stay-green and synchronized maturation groups and elite cultivars. Moreover, to propose promising combinations seeking the selection of high-grain yield and high bread-making quality genotypes containing stay-green trait. The experiment was conducted in a randomized block design with three replications in 2003, 2004, and 2005, using sister-lines with the presence and absence of stay-green trait and elite cultivars. Genetic variability exists among wheat strains from the synchronized stay-green maturation group and elite cultivars. Genotypes of maturation group stay-green obtained an average performance superior to the synchronized group. Crosses between stay-green lines and the CEP 29 and BRS 177 cultivars are promising in the selection of genotypes carrying the stay-green trait with high yield and bread-making quality. 相似文献
108.
Characterization of dinucleotide and trinucleotide EST-derived microsatellites in the wheat genome 总被引:6,自引:0,他引:6
Over the past decade microsatellites or simple sequence repeats (SSRs) have attracted a considerable amount of attention from
researchers. The aim of the present paper was to analyse expressed sequence tag-derived SSR (EST-SSR) marker variability in
wheat and to investigate the relationships between the number and type of repeat units and the level of microsatellite polymorphism.
Two hundred and forty-one new EST-SSR markers available in a public database () were characterized in eight durum wheat cultivars (Svevo, Ciccio, Primadur, Duilio, Meridiano, Claudio, Latino, Messapia),
two accessions of Triticum turgidum var. dicoccoides (MG4343, MG29896), one accession of T. turgidum var. dicoccum (MG5323) and in the common wheat cv. Chinese Spring. Of these, 201 primer pairs (83.4%) amplified PCR products successfully,
while the remaining 40 (16.6%) failed to amplify any product. Of the EST-SSRs analysed, 45.2% of the primer pairs amplified
one or two PCR products. Multiple discrete PCR products were observed among both di- and trinucleotide EST-SSR markers (31.2
and 40.5%, respectively). Markers based on dinucleotide microsatellites were more polymorphic than those based on trinucleotide
SSRs in the 12 wheat genotypes tested (68.9 and 52.7%, respectively). An average of 2.5 alleles for dinucleotide and 2.0 alleles
for trinucleotide SSRs was observed. The data reported in the present work indicate the presence of a significant relationship
between motif sequence types and polymorphism. The primer set based on the AG repeat motif showed the lowest percentage of
polymorphism (55.0%), while the primer set based on the AC repeat motif showed t he highest percentage (85.0%). Among trinucleotide
SSRs, the AGG microsatellite markers showed the highest percentage of polymorphism (70.0%), and the ACG motif the lowest value
(25.0%). The characterization of these new EST-SSR markers and the results of our studyon the effect of repeat number and
type of motifs could have important applications in the genetic analysis of agronomically important traits, quantitative trait
locus discovery and marker-assisted selection. 相似文献
109.
110.
Jorge Durán Alexandra Rodríguez José María Fernández-Palacios Antonio Gallardo 《Biology and Fertility of Soils》2009,45(7):781-788
The concern that climate change may increase fire frequency and intensity has recently heightened the interest in the effects
of wildfires on ecosystem functioning. Although short-term fire effects on forest soils are well known, less information can
be found on the long-term effects of wildfires on soil fertility. Our objective was to study the 17-year effect of wildfires
on forest net mineralization rates and extractable inorganic nitrogen (N) and phosphorus (P) concentrations. We hypothesize
that (1) burned forest stands should exhibit lower net mineralization rates than unburned ones; (2) these differences would
be greatest during the growing season; (3) differences between soil variables might also be observed among plots from different
years since the last fire; and (4) due to fire-resistant geochemical processes controlling P availability, this nutrient should
recover faster than N. We used a wildfire chronosequence of natural and unmanaged Pinus canariensis forests in La Palma Island (Canary Islands). Soil samples were collected during winter and spring at 22 burned and unburned
plots. We found significantly higher values for net N mineralization and extractable N pools in unburned plots. These differences
were higher for the winter sampling date than for the spring sampling date. Unlike extractable N and N mineralization rates,
extractable P levels of burned plots exhibited a gradual recovery over time after an initial decrease. These results demonstrate
that P. canariensis forest soils showed low resilience after wildfires, especially for N, and that this disturbance might induce long-term changes
in ecosystem functioning. 相似文献